Method for treating phenol-containing wastewater using wood chip immobilized Alcaligenes faecalis

A technology of Alcaligenes faecalis and biochemical treatment is applied in the field of microbial catalytic degradation of phenol-containing wastewater, which can solve the problems of low microbial concentration, easy loss of bacterial cells and slow degradation speed, and achieves high microbial concentration, strong tolerance and degradation. fast effect

Inactive Publication Date: 2009-01-21
XIANGTAN UNIV
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  • Summary
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AI Technical Summary

Problems solved by technology

However, there are disadvantages such as easy loss of bacteria, low microbial concentration, slow degradation rate, secondary pollution caused by a large amount of sludge, and large floor area.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Put 5.0kg of 40-mesh sawdust into 15% sodium sulfite solution, adjust the pH to 4.0, soak at 60°C for 48h, and rinse with water. Neutralize with 20% sodium hydroxide solution, wash with water, and dry.

[0017] Introduce Alcaligenes faecalis JH 1013 into a 100L container for routine culture, soak the sawdust in the bacterial solution, the temperature of the bacterial solution is 30°C, pH 7.0, suffocate and fix Alcaligenes faecalis for 48 hours, filter the bacterial solution, and obtain Wood chips immobilize Alcaligenes faecalis.

[0018] Fill the sawdust-fixed Alcaligenes faecalis into the cylindrical reactor in the tower biofilter, inject fresh culture solution, activate the sawdust-fixed Alcaligenes faecalis for 18 hours, and wash away the residual culture solution. It is passed into a chemical plant containing phenol wastewater (the average COD value of the water inlet is 4000mg·L -1 , the phenol concentration at the water inlet is 990mg·L -1 ), the wastewater tem...

Embodiment 2

[0020] Put 5.0kg of 80-mesh sawdust into 20% sodium sulfite solution, adjust the pH to 4.0, soak at 60°C for 48 hours, rinse with water, neutralize with 15% sodium hydroxide solution, wash with water, and dry.

[0021] Introduce Alcaligenes faecalis JH 1013 into a 100L container for routine culture, soak the sawdust in the bacterial solution, the temperature of the bacterial solution is 35°C, pH 7.5, and fix Alcaligenes faecalis for 72 hours, filter the bacterial solution, and obtain Wood chips immobilize Alcaligenes faecalis.

[0022] Fill the immobilized Alcaligenes faecalis into the cylindrical reactor in the tower biofilter, inject fresh culture solution, activate the sawdust immobilized Alcaligenes faecalis for 48 hours, and wash away the residual culture solution. It is passed into a coking plant containing phenol wastewater (the average COD of the water inlet is 5000mg·L -1 , the phenol concentration at the water inlet is 1500mg·L -1 ), the wastewater temperature is 3...

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Abstract

The invention discloses a method for treating phenol-containing waste water by sawdust immobilized alcaligenes faecalis. The method has the advantages that (1) the immobilized alcaligenes faecalis is easy to culture and has the advantages of simple immobilization process, excellent catalytic degradation effect, resistance to high-concentration phenol-containing waste water with the concentration of phenol up to 3000mg.milligram<-1>, strong phenol decomposition capability and repeated use; (2) the carrier of the immobilized alcaligenes faecalis is sawdust, and modified utilization of the sawdust is realized by a chemical method, so the method improves the treatment efficiency for the phenol-containing waste water by means of microbial degradation, and has a broad development prospect; (3) the immobilized alcaligenes faecalis prepared through taking sawdust as the carrier has excellent elasticity, strong resistance to temperature and pH value, stable degradation property and long half life period; and (4) when used for treating the phenol-containing waste water, the sawdust immobilized alcaligenes faecalis has the advantages of simple process, low running cost, moderate conditions, environmental protection, uneasy loss of thalli, high concentration of microorganism inside the carrier, quick degradation speed, high degradation efficiency and no secondary pollution, and can realize continuous industrial degradation of the phenol-containing waste water at the same time.

Description

technical field [0001] The invention relates to a method for catalytically degrading phenol-containing wastewater by using microorganisms. Background technique [0002] Phenol-containing wastewater is a source of water pollution with a wide range of sources, a large quantity, high concentration, and great environmental hazards. It is also one of the key difficulties in water pollution control in my country. Biological degradation of phenol-containing wastewater has the advantages of simple process, mild conditions, environmental friendliness, low treatment cost, and less secondary pollution. Generally 5-500mg·L -1 The phenol-containing wastewater is suitable for biological treatment, but the treatment effect for higher levels of phenol-containing wastewater is not ideal [Sun Min et al., Chinese Journal of Medical Engineering. 2004, 12(3): 93-94; Zhang Jie et al., Sichuan Environment. 2006, 25(1): 8-10]. Free microorganisms can degrade high-concentration phenol-containing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N11/00
Inventor 曾虹燕姜和夏葵廖凯波黄炎王亚举
Owner XIANGTAN UNIV
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