98results about How to "Increase growth activity" patented technology

The invention belongs to the field of biological materials, and relates to a micro-patterning nano-fiber material composited with medicine-carrying MOF and a preparation method and application thereof. According to the material, hydrophilic composite electrostatic spinning nano fiber arranged in a staggered mode is adopted as a skeleton, the micro-patterning structure is arranged in order, and anopen three-dimensional through porous structure is distributed; the hydrophilic composite electrostatic spinning nano fiber is a mixture of a supporting biological compatible material, a hydrophilic biological compatible material and repair active molecule loaded metal organic frame nano-particles. The preparation method comprises the steps that organic solves of the metal organic frame nano-particles with uniformly dispersed loaded repair active molecules, the supporting biological active material and the hydrophilic biological active material are adopted as spinning solutions, and electro spinning is performed for preparation. According to the material, on the basis of the slow-release medicine carrier activity and biological tissue activity, the activity of cells in the engine body environment and micro-patterning nano-fiber material is collaborated and promoted, tissue repair and wound healing are facilitated, and the material is used for preparing a wound healing material.

Disclosed is a method for extracting dihydro quercetin from larch. The invention is an improved production method. Due to different production materials and extraction processes, the dihydro quercetin products in U.S. and European markets are very expensive, at a price of 200-1000 euros per gram. Such a high market price economically limits the wide range of practicability of dihydro quercetin products. During the pretreatment of raw materials, 50 kilograms of larch wood chips and 500-800 liters of 50% ethanol solution are added into a reactor to get fully stirred and mixed; then the mixture is extracted and filtered for the second extraction; the filtrates are combined and adsorbed; the organic solvents are collected and dried by a vacuum evaporator through organic phase vacuum distillation; according to a weight proportion of 10:1, the residues are dissolved with hot water, decolorized with activated carbon and then crystallized at a temperature of 3-5 DEG C to precipitate the dihydro quercetin product which is then crushed after vacuum drying to finished product. The invention is used to extract dihydro quercetin from larch.

The present invention discloses an antibody drug conjugate of a CLDN 18.2-resistant antibody. The structure of the antibody drug conjugate (ADC) is shown as Ab-[(L2)n-L1-D]y in a formula I, wherein Dis a small-molecule cytotoxic drug, L1 and L2 are connected to the drug and the antibody separately, and n is 0 or 1; y represents the average number of D which is coupled to Ab, 0 < y <= 10, and Ab is an antibody which can be specifically bound to human CLDN 18.2, and includes a light-chain variable region (VL) and/or a heavy-chain variable region (VH); and the CLDN 18.2-resistant antibody correspondingly contains at least one specific complementary determining region (CDR) sequence or a mutant sequence of the specific complementary determining region (CDR) sequence in the light-chain variable region (VL) and/or a heavy-chain variable region (VH), and binding of the antibody to CLDN 18.2 is maintained or improved through the mutation. The invention also discloses a ADC-containing pharmaceutical composition and a preparation method and application of ADC. The antibody drug conjugate of the antibody has a large security window and low toxic and side effects, and provides more specific,more effective and better treatment option for tumor patients.

The invention belongs to the technical field of biomedicines, and in particular relates to application of seaweed polysaccharides. The seaweed polysaccharides can be taken as preparations for preparing anti-viral or immune medicines. Cell models and in-vivo animal experiments show that the seaweed polysaccharides can be used for significantly enhancing the animal immunity, and can also be used for promoting the generation of cell factors, the typing of T lymphocytes and the proliferation of mouse spleen cells so as to ensure that cellular immune reaction can be activated. The functional seaweed polysaccharides disclosed by the invention are natural polysaccharides which are extracted from seaweeds such as large seaweeds and kelps, gulfweeds, grateloupia sparsa, eucheuma muricatum, ulva pertusa kjellm, enteromorpha, gracilaria, ascophyllum nodosum and scytosiphon lomentaria, and can also be low-molecular-weight seaweed polysaccharides or seaweed oligosaccharides which are prepared by degrading the seaweed polysaccharides by virtue of different preparation methods. According to the application disclosed by the invention, a polysaccharide extract of single seaweed or a polysaccharide extract mixture of a variety of seaweeds can be taken as a novel anti-viral and immune enhancing agent to be applied to feeds of livestock, poultry, fish, shrimps and shellfish, and has wide application prospects.

The invention relates to a decalcified dental matrix of a mammal and a preparation method thereof. The raw material is from permanent teeth of the mammal. The preparation method comprises the following steps: (1) collecting permanent teeth of the mammal for later use; (2) soaking the permanent teeth in a 50% 84 disinfectant for sterilizing, wherein the time for sterilizing is 45 minutes and the temperature is 35-40DEG C; (3) removing useless parts of the teeth; (4) smashing tooth tissues into particles; (5) sterilizing the smashed tooth tissue particles, wherein the time for sterilizing is 45 minutes and the temperature is 35-40DEG C; (6) putting the treated tooth tissue particles into 0.65-0.72 equivalent weight of hydrochloric acid liquid for soaking for 10-16 hours at 34-38DEG C to finish decalcification; and (7) drying at low temperature, soaking in hydrogen peroxide for 30 minutes, so as to obtain the decalcified dental matrix of the mammal. The decalcified dental matrix of the mammal has better inductive bone regenerative action, and does not carry any pathogenic factor after being prepared into final products.

The invention discloses culture media for edible mycorrhizal fungi and symbiotic seedlingsand and a synchronous culture method by utilizing the culture media. The culture media for the edible mycorrhizal fungi and the symbiotic seedlings comprise an edible mycorrhizal fungus strain mycelium culture medium and an edible mycorrhizal fungus symbiosis seedling synthesis culture medium, and the ediblemycorrhizal fungus strain mycelium culture medium includes a solid culture medium used for expanding reproduction of the mycelium and a liquid culture medium used for culturing mycelium which is usedfor inoculation of the mycorrhizal symbiotic seedlings. The synchronous culture method for implementing edible mycorrhizal fungus symbiotic seedling synthesis by utilizing the above culture media comprises the following steps: (1) a cultivation method for the edible mycorrhizal fungus strain mycelium, (2) a cultivation method for the edible mycorrhizal fungus symbiotic seedlings. According to theinvention, cultivation of the edible mycorrhizal fungus mycelium is divided into two stages including solid culture and liquid culture, liquid fungus strains are adopted for inoculation, and meanwhilethe edible mycorrhizal fungus mycelium and symbiotic plants in the mycorrhizal seedling synthesis culture medium grow together, so that the infection ability of the mycelium is increased by 30% or more, the success rate of the inoculation is increased by 20%-30%, and the survival rates of symbiotic plant seedlings and the mycorrhizal fungus mycelium are both increased by 20% or more.

Disclosed is a preparation method for extracting dihydro quercetin from larch. The invention is an improved production method. Due to different production materials and extraction processes, the dihydro quercetin products in U.S. and European markets are very expensive, at a price of 200-1000 euros per gram. Such a high market price economically limits the wide range of practicability of dihydro quercetin products. During the pretreatment of raw materials, 50 kilograms of larch wood chips and 500-800 liters of 50% ethanol solution are added into an extractor to get fully stirred and mixed; then the mixture is extracted and filtered for the second extraction; the filtrates are combined and adsorbed; the organic solvents are collected and dried by a vacuum evaporator through organic phase vacuum distillation; according to a weight proportion of 10:1, the residues are dissolved with hot water, decolorized with activated carbon and then crystallized at a temperature of 3-5 DEG C to precipitate the dihydro quercetin product which is then crushed after vacuum drying to finished product. The invention is used to extract dihydro quercetin from larch.

The invention relates to a benzimidazole zinc complex and a preparation method thereof. A chemical name of the benzimidazole zinc complex is dichloro-2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole zinc complex. The preparation method includes following steps: mixing 2-(carboxymethyl)benzoic acid with o-phenylenediamine, placing the mixture in phosphoric acid, performing a heating process at 180 DEG C for 3 hours to obtain a purple-green reactant, cooling the reactant to room temperature, adding distilled water, performing a neutralization reaction to obtain a yellow-green solid, performing a washing process through distilled water for three times, carrying out a filtering process, carrying out a recrystallization process through alcohol to obtain a yellow-green solid 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole, namely a ligand, dissolving ZnCl2 and the ligand 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole in a proper amount of a DMF solvent to obtain a mixture, stirring the mixture uniformly at room temperature, performing a volatilization process for seven days to obtain a slight yellow-green block-shaped complex monocrystal. The complex employs biological non-toxic element zinc as a metal ion, can be used for preparing antitumor drugs, can inhibit generation of esophagus cancer cells, and is free from remaining in human body and causing poisoning in human body due to metal accumulation. The complex is simple in the preparation method, is high in yield, is low in cost and is suitable for an industrial production.

The invention discloses a mutagenic screening method of a DHA-producing strain crypthecodinium. cohnii. According to the invention, a protoplast of a DHA-producing strain crypthecodinium. cohnii ATCC30556 is prepared; single or composite mutagenesis of ultraviolet + LiCl and diethyl sulfate is carried out upon the protoplast; a basic thought of metabolism regulation and fermentation cultivation is adopted, and oriented screening is carried out by using malonic acid and/or a high-glucose resistance plate; and preliminary screening is carried out by using a solid cultivation medium of red tetrazoline (TTC), such that screening efficiency can be effectively improved, screening links can be reduced, and work load can be reduced. The strain obtained from the preliminary screening step is processed through shake-flask secondary screening and hereditary stability verification. As a result, the growth activity of the strain after mutagenesis is improved. Compared to an initial strain, the DHA biosynthesis amount of an YVD-4 mutagenic strain is improved by 4.7 times, and reaches 45.3g/L. Therefore, the method has an excellent prospect of industrialized production.

The invention belongs to the technical field of bio-fertilizer and saline-alkali soil improvement. The invention discloses a bio-fertilizer for improving moderate-severe saline-alkali soil as well asa preparation method and application thereof. According to the invention, the bio-fertilizer is prepared by mainly adding mainly a compound microbial agent of a specific salt-tolerant strain, a mixedorganic fertilizer, wormcast, a water-retaining agent, a nitrogen fertilizer and a calcium fertilizer; the problems of low survival efficiency, short action time and low crop growth of strains in saline-alkali soil with the salt content of 0.6% or above are solved, and the salt content of moderate-severe saline-alkali soil is reduced, organic matters are increased and crop growth is improved. Theproduct is mainly salt-tolerant nutritional strains and metabolites thereof, has the characteristics of strong strain activity, long lasting action time, soil fertility improvement, soil aggregate structure increase and crop growth promotion, and improves the salt conversion and fertility improvement in the soil through the strain propagation effect, thereby finally realizing the crop yield increase.

The invention relates to the technical field of gastrodia elata planting, in particular to a gastrodia elata planting method. Through treatment of the steps (1), (2), (3) and (4), nutrition supply in the gastrodia elata growth process is sufficient, the contact rate of protocorms and armillaria mellea is high, the growth vigor of the protocorms is high, few gastrodia elata capsules are generated, and the yield of finished gastrodia elata is high.

The invention relates to the technical field of vegetable planting, in particular to a germination accelerating seedling culture method of tomatoes. The method comprises the following steps: soaking mature seeds which are full in size in sweet potato juice, scattering the seeds onto wet gauze, covering the seeds with fine river sand, and accelerating the germination, wherein various active ingredients in the sweet potato juice can effectively activate the activity of the tomato seeds and also have a sterilizing effect, the seed germination rate can be effectively increased to 95 percent, the growth activity of the tomato seedling is increased from the aspect of the seeds, and the growth effect of the tomatoes is improved; and the seedling is cultured after the germination acceleration, byadopting special nutritional soil and a seedling culture environment, the tomato seedling begins to grow from a same growth point, the growth advantages of the tomato seedling can be effectively improved, the growth is homogenous, the seedling survival rate is 98 percent, the transplanting survival rate of the tomato seedling is 99 percent, the growth period is averagely shortened by 2.5 weeks, the tomatoes can be available on market in advance, the market demand can be satisfied, and the economic income of the growers can be increased.

The invention discloses a vegetable planting method, and relates to the field of agricultural planting. The method comprises the steps that a seedling raising matrix is spread in a seedling raising plate; vegetable seeds are uniformly broadcast in the seedling raising matrix, and the seedling raising plate in which the vegetable seeds are broadcast is placed in a greenhouse with sufficient sunlight, wherein the seed sowing line spacing is 40 cm, the seed sowing depth is 4 cm, and the seeds are covered with a little amount of straw; on the first day that the vegetable seeds are broadcast, water is sprayed once every three hours, and fertilizer is applied once every day; on the second day to the tenth day, water is sprayed once every six days, and fertilizer is applied once every day; on the eleventh day to the thirty-fifth day, water is sprayed once every five days, and fertilizer is applied once every day; after thirty five days, vegetables are preliminarily cultivated, and then field planting is conducted.

The invention relates to a low-temperature high-efficiency artificial moss wetland system, which comprises a bed body, a substrate layer and moss plants, wherein a perforated water distribution pipe is arranged on one side of the bed body and is connected with a water inlet pipe, a perforated collecting pipe is arranged on the other side of the bed body and is connected with a water outlet pipe, a length-width ratio of the bed body is 2:1 to 10:1, and the height is between 30 and 40 centimeters; the substrate layer is positioned in the bed body and consists of a bottom layer, a middle layer and an upper layer, the thickness of the bottom layer is between 4 and 5 centimeters, the thickness of the middle layer is between 10 and 20 centimeters, the middle layer is formed by combining soil and fine sand, and a mixing ratio is 2:1-3:1; the thickness of the upper layer is between 10 and 20 centimeters, the upper layer is formed by combining antarctic moss wetland surface layer underlying soil and local soil, and a complex ratio is 1:1-1:2; the moss plants are positioned above the substrate layer, the height of the moss plants is between 7 and 10 centimeters, the planting density is between 1*10<5> and 4*10<5> plants/m<2>, and the moss plants are selected from Bryum muehlenbeckii; and a baffle which is parallel or perpendicular to the water inflow direction can be arranged inside the bed body. The low-temperature high-efficiency artificial moss wetland system can be used for the treatment of domestic sewage in low-temperature areas, and has the excellent treatment effect on main pollutants such as ammonia nitrogen, organic matters, total phosphorus, suspended matters and the like under the low-temperature condition of below 10 DEG C.

The invention relates to a crucian breeding quality improvement method. The crucian breeding quality improvement method comprises the following steps: 1, selecting parents; 2, constructing a pool; 3, cultivating the parents; 4, injecting hormone; 5, artificially fertilizing; and 6, hatching fertilized eggs. By improving the crucian growing environment and the manual injection and fertilization fishing ways, the method can improve the anti-stress capability of crucian and reduce the stimulation to the crucian and is beneficial to growth and breeding of the parents, the yield of the bred crucian is increased by 10-15%, and the survival rate of the fish fry is increased by 8-12%.

The invention discloses a grape grafting technology, and relates to the technical field of grape cultivation, and solves a problem that existing grape hard branch grafting is low in survival rate. Thetechnology comprises steps: (1) preparing stocks and scions; (2) preparing grafting; (3) grafting; (4) performing later management. Compared with conventional hard branch grafting, grape green branches are used to graft in the method, green branches have better growth activity than hard branches, and have higher survival rate and growth velocity, so that breeding speed of grapes is greatly improved.

The invention provides a method for quickly planting bamboo shoots. The method mainly comprises the steps that material selection and treatment, management of a cultivation bed at the early stage, cultivation, management of the cultivation bed at the later stage, planting at the forest and maintenance and caring at the later stage; according to the material selection and treatment, high-quality branch strips of the bamboo shoots are selected, cleaned with clear water three times firstly, soaked in warm water with the temperature of 18-23 DEG C for 20-30 minutes and then soaked in disinfectionliquid for 25-29 seconds; finally the branch strips are placed and soaked in chemical liquid for 10-15 hours and drained off for use. The method for quickly planting the bamboo shoots has the advantages that scientific planting is achieved, the bamboo shoots with good growth vigor and few germs can be quickly cultivated, the cultivation period is shortened, and the economic benefit is increased.

A plant growth regulator composition is disclosed. The composition includes an active component A that is sodium tungstate and an active component B that is 5-aminolevulinic acid in a weight ratio of1:(0.5-2.8). A preparation including the composition is also provided in which the total weight percentage of the active component A is 0.1-50%, and in which the total weight percentage of the activecomponent B is 0.1-50%. The dosage form of the preparation is any one of an aqueous agent, soluble powder, soluble particles, powder, tablets and wettable powder. The composition and the preparation can be used for plant photosynthesis and respiration, can break seed dormancy and can improve fruit yield and quality.

The invention discloses a two-stage short-cut nitrification and anaerobic ammonia oxidation coupled endogenous denitrification deep denitrification device and a method thereof. The device comprises araw water tank, a PN reactor, an intermediate water tank and an SAED reactor, urban sewage raw water is stored in the raw water tank, the PN reactor is connected with the raw water tank through a PN water inlet pump, the PN reactor is connected with the middle water tank through a PN water outlet valve; the municipal sewage raw water in the raw water tank is further connected to the SAED reactor through a SAED water inlet pump, the SAED reactor is connected with the middle water tank through the short-cut nitrification liquid inlet pump, and the municipal sewage raw water and short-cut nitrification liquid in the SAED reactor are subjected to deep denitrification and then discharged through a water outlet valve of the SAED reactor. The anaerobic ammonia oxidation coupling endogenous denitrification is taken as a main denitrification way, and the method has the advantages of no need of adding an external carbon source, economy, high efficiency, stable operation and the like, and is suitable for the technical fields of construction of small and medium-sized urban sewage treatment plants, upgrading and reconstruction of constructed projects and the like.

The invention discloses a cress planting method, and relates to the field of agricultural planting. The method comprises the steps that seeding substrates are laid in a seeding plate; cress seeds are evenly sown on the seeding substrates, and the seeding plate sown with the cress seeds is placed in a greenhouse with full sunshine; in the first day after the cress seeds are sown, water is sprayed for 60 seconds every two hours; in the second day to the tenth day, the water is sprayed for 60 seconds every six hours; in the 11th day to the 35th day, nutrient solutions are sprayed for 60 seconds every five hours, cress is preliminarily bred after 35 days, and then field planting is performed to increase the survival rate.