Application of carbon quantum dot-graphite phase like carbon nitride photocatalytic material to preparation of medicines capable of killing bacteria and promoting skin cicatrice healing

A graphite phase carbon nitride and phase carbon nitride light technology, applied in the field of photocatalytic materials, can solve the problems of low energy consumption, production of carcinogenic by-products, low efficiency, etc., and achieves strong photocatalytic activity, obvious bactericidal effect, and considerable The effect of applying value

Active Publication Date: 2019-04-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because it can avoid some disadvantages, such as low energy consumption, low efficiency and generation of carcinogenic by-products, etc.

Method used

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  • Application of carbon quantum dot-graphite phase like carbon nitride photocatalytic material to preparation of medicines capable of killing bacteria and promoting skin cicatrice healing
  • Application of carbon quantum dot-graphite phase like carbon nitride photocatalytic material to preparation of medicines capable of killing bacteria and promoting skin cicatrice healing
  • Application of carbon quantum dot-graphite phase like carbon nitride photocatalytic material to preparation of medicines capable of killing bacteria and promoting skin cicatrice healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1CQD s / g -C 3 N 4 preparation of

[0047] 1. Graphite Carbon Nitride (Graphite Carbon Nitride, g-C 3 N 4 )Synthesis

[0048] Put urea (30g) in a ceramic crucible with a lid, and calcined at 550°C for 2 hours at a heating rate of 4°C / min to obtain a light yellow powder, namely g-C 3 N 4 .

[0049] 2. Synthesis of Carbon Quantum Dots (CQDs)

[0050] Dissolve 3g of citric acid and 1mL of ethylenediamine in 30mL of deionized water to obtain a mixed solution I; then transfer the mixed solution I to a 50mL hydrothermal synthesis reactor and heat at 200°C for 5 hours; after the reaction, the reaction The device was naturally cooled to room temperature, and dried at 80° C. for 24 hours to obtain a dark brown powder, which was carbon quantum dots.

[0051] 3. Carbon quantum dots-graphite-like carbon nitride (CQDs / g-C 3 N 4 )Synthesis

[0052] Mix 6mg CQDs with 30mL water to obtain mixed solution II, then add 200mg g-C 3 N 4 Dispersed in mixed solution II; ...

Embodiment 2

[0062] Embodiment 2 carbon quantum dots-like graphitic phase carbon nitride (CQDs / g-C 3 N 4 ) in killing Staphylococcus aureus

[0063] 1. Combine CQDs / g-C 3 N 4 Interact with SA to detect the survival of SA. Samples were taken every 20 minutes, and the number of SA survivors was calculated by standard plate count method.

[0064] S. aureus was cultured in tryptic soy broth (TSB) medium and then resuspended in phosphate buffered saline (PBS, 0.01 M, pH 7.4) to construct the final S. aureus suspension (10 7 CFU mL -1 ). For each sterilization experiment, 50 mL of Staphylococcus aureus suspension and 50 mg of photocatalyst powder were mixed in a beaker. The bacteria and photocatalyst were uniformly mixed on a magnetic stirrer at room temperature while irradiating with a 300W xenon lamp equipped with a UV cut filter (λ-2 . As the reaction proceeds, the mixture is carefully pipetted at predetermined intervals and the bacterial concentration is determined by plate counting....

Embodiment 3

[0071] Embodiment 3 carbon quantum dots-like graphitic phase carbon nitride (CQDs / g-C 3 N 4 ) in the repair of Staphylococcus aureus infected skin

[0072] 1. Divide 6-8 week old C57BL / 6 female mice into untreated group, CQDs / g-C 3 N 4 group and g-C 3 N 4 Group.

[0073] First, a 1.5% amobarbital sodium solution was prepared, and mice were anesthetized by intraperitoneal injection in an amount of 120 μl / 20 g to construct a SA skin infection model.

[0074] Then, 80mm of skin was made on the back skin of mice in each group. 2 area of ​​sterile wound, place 1 x 10 7 CFU of Staphylococcus aureus smeared on the window, followed by 100mg of CQDs / g-C on the back 3 N 4 or g-C 3 N 4 , irradiated for 2 hours under light conditions.

[0075] The area and bacterial load of skin scars in each group were observed.

[0076] It was found that: with g-C 3 N 4 Group comparison, CQDs / g-C 3 N 4 Skin scars healed faster in mice in the group (see Figure 6 ).

[0077] 2. Combine...

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Abstract

The invention discloses an application of a carbon quantum dot-graphite phase like carbon nitride photocatalytic material to preparation of medicines capable of killing bacteria and promoting skin cicatrice healing. A preparation method of the material comprises the following steps of placing urea at the temperature of 500-600 DEG C for calcination so as to obtain graphite phase carbon nitride powder; enabling citric acid and ethylenediamine to dissolve in water, performing a hydrothermal synthesis reaction at 180-200 DEG C, performing cooling, and performing drying so as to obtain carbon quantum dot powder; and mixing the carbon quantum dot powder with water, adding the graphite phase carbon nitride powder to mixed liquor, performing stirring and macerating at the temperature of 15-60 DEGC for 12-24 hours, and performing drying so as to obtain the catalysis material. According to the application disclosed by the invention, an in vitro staphylococcus aureus infection model and an in vitro staphylococcus aureus infection model are constructed, the inventor finds that the carbon quantum dot-graphite phase like carbon nitride photocatalytic material prepared by a specific method hasmore photocatalytic reaction active sites, is higher in photocatalytic activity and is obvious in the effect of killing staphylococcus aureus.

Description

technical field [0001] The invention relates to the technical field of photocatalytic materials, in particular to the application of carbon quantum dot-like graphite phase carbon nitride photocatalytic materials in the preparation of medicines for sterilization and skin scar healing. Background technique [0002] Staphylococcus aureus (abbreviated as Staphylococcus aureus, SA) is an important Gram-positive pathogen that can secrete a variety of invasive substances, including virulence factors and invasive proteins. SA can cause infections in skin and soft tissues, lungs, bones and joints, and blood, and the resulting infectious diseases include pneumonia, sepsis, endocarditis, skin and soft tissue infections, and even toxic shock syndrome. SA can produce resistance to multiple antibiotics through multiple drug resistance mechanisms such as gene mutation and horizontal transfer of self-resistant genes. At present, multidrug-resistant SA has become a clinically important path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61P31/04A61P17/02
CPCA61K41/0023A61P17/02A61P31/04
Inventor 徐峰夏靖燕
Owner ZHEJIANG UNIV
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