26results about "Agression altered materials" patented technology

The invention belongs to the technical field of medicines, and relates to injectable piezoelectric hydrogel for a spinal cord injury part as well as a preparation method and application of the injectable piezoelectric hydrogel. According to the hydrogel, methacrylated gelatin is used as a carrier, and a piezoelectric material MOF (at) PDA (at) M0, namely MP (at) M, of an enveloping porous structure is loaded in the hydrogel. The hydrogel can achieve dual functions under the ultrasonic action: (1) MP (at) M has piezoelectricity, ultrasonic mechanical energy is converted into electrical stimulation, calcium ion inward flow of astrocytes is triggered, mitochondria is promoted to be transferred from the astrocytes to neurons, and the mitochondrial function of the neurons is recovered; (2) the CD73 enzyme carried by the microglial cell membrane coated on the surface of the MP (at) M can degrade pro-inflammatory ATP (adenosine triphosphate) at the damaged part to generate adenosine, so that astrocytes are promoted to release lactic acid, and rapid energy support is provided for neurons. Mitochondrial transfer and energy supply are synchronously promoted through ultrasonic stimulation, neuronal plasticity is remodeled, spinal cord axon regeneration is promoted, and a new strategy is provided for spinal cord injury treatment.

The present disclosure includes a system for treating cancer, the system comprising: an extracorporeal circulation device that is for collecting and returning the blood of a subject, and that includes a blood circuit and a blood pump; and a light irradiation device that is for irradiating the blood circuit with light. A photosensitive substance is administered to the subject before blood collection or is added, before light irradiation, to blood collected from the subject. The blood that has been collected from the subject and has been irradiated with light is returned to the subject. The irradiation energy density of the light is 2.5-300 J / cm2.

The invention discloses a zinc oxide nanosheet and a preparation method and application thereof. The zinc oxide nanosheet is of an ultrathin sheet structure with the thickness of 3-6 nm and has piezoelectric catalytic activity. The preparation method comprises the following steps: dissolving zinc salt and hexamethylenetetramine in a high-volume-fraction ethanol water solution, and stirring at 55-65 DEG C for reaction. The nanosheet is applied to preparation of drugs for destroying bacterial biofilms or treating implant-related infections, and needs to be combined with ultrasonic irradiation. Under the ultrasonic action, the nanosheets generate a piezoelectric catalytic effect, lactic acid in a biological membrane microenvironment can be degraded, immunosuppression is relieved, and macrophages are promoted to be polarized to a pro-inflammatory phenotype; meanwhile, an ultrasonic cavitation effect is combined, so that a biological membrane can be synergistically and efficiently removed and bacteria can be killed.

The invention belongs to the technical field of biomedical materials, and particularly relates to ultrasonic-excited piezoelectric delivery exosome electrostatic spinning and a preparation method thereof.The piezoelectric delivery exosome electrostatic spinning comprises piezoelectric electrostatic spinning prepared by embedding barium titanate nanoparticles into polylactic acid, the surface of the piezoelectric electrostatic spinning is modified by chitosan and then loaded with exosome, and the surface of the piezoelectric electrostatic spinning is loaded with the exosome. The preparation method comprises the following steps: preparation of piezoelectric electrostatic spinning: dissolving polylactic acid in hexafluoroisopropanol, adding barium titanate nanoparticles, and carrying out ultrasonic dispersion to form a uniform precursor solution; piezoelectric electrostatic spinning containing barium titanate is prepared through high-voltage electrostatic spinning; piezoelectric electrostatic spinning surface modification: immersing the piezoelectric electrostatic spinning into a chitosan solution for incubation, and modifying the piezoelectric electrostatic spinning to form piezoelectric electrostatic spinning with positive electricity on the surface; the modified piezoelectric electrostatic spinning is soaked in an exosome suspension to be incubated, and piezoelectric exosome delivery electrostatic spinning is prepared and has the self-energy-supply function, the space-time response function and the biological delivery function, the defects of traditional biological medicine materials are hopefully overcome, and the piezoelectric exosome delivery electrostatic spinning can be applied to the field of biological medicine materials. The clinical transformation application of the intelligent biological material in the chronic wound repair of diabetes mellitus is promoted.

The invention discloses efficient tumor sonodynamic therapy nanoparticles based on carbon-doped nano-zinc oxide as well as a preparation method and application of the efficient tumor sonodynamic therapy nanoparticles. Belongs to the field of biomedicine. On the basis of MOF-5, carbon-doped hollow-hole-shaped nano-particles C-ZnO are formed after calcination in a nitrogen atmosphere, then chloroplatinic acid hexahydrate and sodium borohydride are added into a C-ZnO nano-particle solution, so that Pt nano-particles are loaded on the surface of C-ZnO, in addition, AB is loaded in a CZP hollow structure, HA-AA modification is carried out on the surface of the CZP hollow structure, and the CZP hollow structure loaded with the AB is obtained. Therefore, the effects of hydrogen treatment, consumption of excessive glutathione in a biological microenvironment and targeting are achieved, and the effect of treating cancers is achieved from multiple aspects. The nano particles are uniform in size, the average particle size is about 200 nm, good biocompatibility is achieved, and the material prepared through the method has excellent ROS generation capacity, so that the material has excellent sonodynamic therapy potential and has wide application prospects in the field of tumor therapy.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

Provided herein are compositions including biocompatible magnetizeable nanoparticles. The nanoparticles have a diameter (average diameter) from about 10 to about 300 nanometers and are biocompatible and magnetic. The nanoparticles may be a disc formed from iron oxide. The disc may be conjugated to a target-binding moiety capable of binding a target. The target may be cancer cells, pathogens, fat cells, or atherosclerotic plaques.

The invention provides a CO intelligent release platform based on a flexible implantable circuit and NFC induction. A forming method of the CO intelligent release platform comprises the following steps of constructing an NFC-LED module, forming a PDMS substrate, preparing a photosensitive composite layer, integrating devices and wirelessly activating a system. According to the CO intelligent release platform based on the flexible implantable circuit and NFC induction, a controllable CO release system based on a (carbon nitride / gold) composite nano-catalyst is constructed, and multiple mechanisms such as wireless NFC activation, blue light driving, nano-material directional reaction, release rate accurate regulation and control, targeted therapy and the like are combined; the defects of poor drug release accuracy, strong activation mode trauma, non-adjustable release rate, indefinite treatment window, high system energy consumption and the like in the prior art are overcome.

The application relates to the technical field of composite piezoelectric materials, and particularly discloses a composite nano piezoelectric material loaded with maslinic acid, a preparation method and application thereof, which comprises the following steps: adding barium titanate nano powder and maslinic acid into a solvent in sequence, performing ultrasonic treatment, then stirring and treating at 50-70 DEG C for 1.5-3h to obtain a mixture; adding PVDF powder into the mixture, stirring and treating at 50-70 DEG C for 4-6h to obtain a final mixed solution; meanwhile, washing and drying a glass slide to obtain a treated glass slide substrate; coating the mixed solution on the glass slide to form a film, drying to obtain a precursor film; and performing polarization treatment on the film to obtain the composite nano piezoelectric material loaded with maslinic acid. By introducing the barium titanate and the maslinic acid, the application effectively induces the PVDF to form a high-content polar beta crystal phase, thereby significantly improving the piezoelectric performance of the PVDF, and meanwhile, the flexibility of the PVDF film is also enhanced, the wearable patch substrate is suitable, and the application exhibits good drug controlled release potential.

The application provides a bismuth-doped sodium niobate / oxygen vacancy ceria heterojunction and a preparation method and application thereof. The bismuth-doped sodium niobate / oxygen vacancy ceria heterojunction comprises the following raw material components in moles: 3-5 moles of bismuth-doped sodium niobate; and 1 mole of oxygen vacancy ceria. The bismuth-doped sodium niobate / oxygen vacancy ceria heterojunction has strong piezoelectric properties and acoustic power properties, and has enzyme-like activity. Under the driving of ultrasonic waves, the piezoelectric effect of the material not only promotes the proliferation of osteoblasts, but also enhances the acoustic power and enzyme-like activity of the material, generates various ROS, cooperatively kills bacteria and removes biofilms, and has potential application prospects in the treatment of infected bone tissues.

Methods and systems for improving or enabling delivery of antibodies, immunoglobulin receptors, or immunotherapy agents across the Blood-Brain Barrier (BBB) are disclosed. Exosomes are derived from stem cells, immune cell progenitors, or immune cells, and are loaded with an antibody, immunoglobulin receptor, or antibody fragment. Focused energy, preferably low-intensity focused ultrasound, is applied to a region of the BBB of a patient, and loaded exosomes are administered to the patient. Exosomes target the region for delivery of the loaded antibody, receptor, or fragment, either by traversing the BBB at the region or releasing the loaded antibody to neural tissue at the region.

This invention relates to the field of peptide drugs, specifically to the field of ultrasound physical field-mediated regulation of biological signals and bone tissue repair technology, and more specifically to a piezoelectric hydrogel loaded with responsive peptides and its application. The application is based on a biological signal regulation method of ultrasound-induced responsive peptides LLPS and its application in promoting bone differentiation. It achieves spatiotemporal specific activation of biological signal pathways through precise regulation mediated by physical fields.

Disclosed herein is a method of promoting drug uptake via TRPC1 channels using a pulsed electromagnetic field (PEMF). Also disclosed herein are methods of treating cancer using PEMF adjuvant therapy and methods of selecting cancer patients for PEMF adjuvant therapy by detecting TRPC1 levels in a cancer sample.

The invention discloses a FeMOF / PtCu heterojunction sound-sensitive agent as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The sound-sensitive agent is endowed with piezoelectric property by constructing a heterojunction structure between FeMOF and PtCu, so that the sound-sensitive agent not only can efficiently catalyze hydrogen peroxide to generate active oxygen under the ultrasonic action, but also can generate oxygen through Pt catalysis, and meanwhile, cells are synergistically induced to generate ferroptosis and copper death through exogenous release of Fe < 2 + > and Cu < 2 + >; the preparation method comprises the steps of hydrothermal synthesis of FeMOF, thermal decomposition preparation of PtCu and compounding of FeMOF and PtCu through surface modification, and the prepared heterojunction sound-sensitive agent has good sonodynamic performance and metal ion release characteristics, shows remarkable active oxygen generation capacity and cytotoxicity to 4T1 cells under in-vitro conditions, and can be used for preparing the heterojunction sound-sensitive agent. And a novel multifunctional nano material for reference is provided for the field of sonodynamic therapy.

This invention relates to the field of biomedical technology, and more particularly to the application of low-intensity pulsed ultrasound in promoting the uptake of exosomes carrying chemotherapeutic drugs by tumor cells. The invention uses an intensity of 200 mW / cm². 2 ~350mW / cm 2 Low-intensity pulsed ultrasound, without damaging exosomes carrying chemotherapeutic drugs, promotes the uptake efficiency of these drugs by tumor cells, thereby enhancing the therapeutic effect of chemotherapeutic drugs on tumor cells. This invention, through cell and animal experiments, demonstrates that low-intensity pulsed ultrasound combined with exosomes carrying chemotherapeutic drugs can enhance the therapeutic effect of chemotherapeutic drugs on tumor cells, providing new theoretical and experimental basis for tumor treatment and showing promising application prospects.

The invention relates to the field of polypeptide medicines, in particular to the technical field of regulation and control of biological signals and bone tissue repair by an ultrasonic physical field, and particularly relates to piezoelectric hydrogel loaded with response polypeptide and application thereof, and the application is a biological signal regulation and control method based on ultrasonic induced response polypeptide LLPS and application of promoting bone differentiation. Through precise regulation and control mediated by a physical field, space-time specific activation of a biological signal channel is realized.

The application relates to the technical field of biology, and particularly relates to a sound electricity Ba that triggers post-translational modification 0.85 Ca 0.15 Ti 0.9 Yb 0.1 O3 nanoparticles, a preparation method and application thereof. A titanium tetrachloride solution and a mixed reagent solution are stirred and mixed to obtain a stirring mixed solution; the stirring mixed solution is mixed with sodium hydroxide and polyvinylpyrrolidone and then subjected to heat treatment to obtain a heat-treated product; the heat-treated product is subjected to solid-liquid separation to obtain a solid, and the solid is washed to be neutral and dried. + The nanoparticles provided by the application consume H + around mitochondria under ultrasonic stimulation, leading to depolarization of a mitochondrial membrane potential. After the mitochondrial membrane potential is depolarized, E3 ubiquitin ligase Parkin is transferred from a cytoplasm to a mitochondrial outer membrane, and is massively aggregated and accumulated on the mitochondrial outer membrane, leading to ubiquitination modification and cascade degradation of an anti-apoptotic protein MCL-1 of the mitochondrial outer membrane, so that cell apoptosis is promoted.

A skin treatment system that uses ultrasound devices, and more particularly, handheld devices with an ultrasound transducer used in combination with a continuous flow of serum carrying acoustically responsive media wherein acoustic energy transmitted from the transducer causes dimensional changes in the acoustically responsive media to create acoustic pressure peaks capable of loosening paracellular junctions in the skin to enhance penetration of serum into the skin.

The invention discloses an ultrasonic piezoelectric composite material and a preparation method thereof. The ultrasonic piezoelectric composite material comprises a first thin film layer and a second thin film layer which are laminated, the first thin film layer and the second thin film layer are made of a polymer doped with barium titanate; the first thin film layer is a disordered fiber spinning film, and the second thin film layer is an ordered fiber spinning film; a magnesium-containing compound is loaded on the surface of the first thin film layer and / or the second thin film layer, or a magnesium oxide layer is deposited on the surface of the first thin film layer and / or the second thin film layer. The composite material can generate a bio-electricity signal by applying non-interventional ultrasonic induction simulation, and meanwhile, release of a magnesium-containing compound or a magnesium oxide layer loaded on the surface of the piezoelectric material is regulated and controlled through ultrasonic, so that tissue repair is promoted.

In one aspect, a method of preconditioning stem cells comprising exposing stem cells to low dose radiation (LDR) is provided. In another aspect, a population of preconditioned stem cells is provided, wherein the population of 5 preconditioned stem cells is obtained by exposing stem cells to LDR. Uses of the preconditioned stem cells are also provided. In other aspects, the stem cells are muscle stem cells.

The invention provides an internal friction network piezoelectric hydrogel microsphere as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the preparation method, supramolecular engineering and a microfluidic strategy are utilized, and a hydrogel microsphere system of an internal friction network is constructed through cooperative assembly of piezoelectric bismuth ferrite nanoparticles (BFs) and slip ring functionalized methacrylated hyaluronic acid (HAMA), so that physiological electroadaptation in degeneration tissues is realized. The BFs convert mechanical stimuli into electrical signals, while a stress-dependent internal friction network modulates energy dissipation. Under low stress, the slip ring moves to generate low friction, so that electric signal generation is enhanced; under high stress, the main chain is straightened, so that friction is increased, excessive signals are inhibited, and physiological electric adaptation is recovered. The microspheres can generate a stable electric field under dynamic loading, mitochondrial autophagy is promoted by activating a PINK1 / Parkin pathway, stable mitochondrial membrane potential is maintained, and nucleus pulposus cell apoptosis is reduced by 75%.

Provided herein are compositions including biocompatible magnetizeable nanoparticles. The nanoparticles have a diameter (average diameter) from about 10 to about 300 nanometers and are biocompatible and magnetic. The nanoparticles may be a disc formed from iron oxide. The disc may be comjugated to a target-binding moiety capable of binding a target. The target may be cancer cells, pathogens, fat cells, or atherosclerotic plaques.

The invention discloses a barium titanate composite nanomaterial for ultrasonic piezoelectric catalysis and treatment of drug-resistant recurrent glioma, and belongs to the field of glioma treatment. The preparation method comprises the following steps: firstly, preparing BaTiO3 nano-particles, then sequentially wrapping the surfaces of the BaTiO3 nano-particles with polydopamine (PDA), and loading peroxymonosulfate to obtain the composite piezoelectric material. The obtained composite piezoelectric material can regulate and control the internal and external electronic structures of the piezoelectric material at the same time to enhance activation of peroxymonosulfate and generate. SO4, so that synergistic ultrasonic treatment of glioblastoma is realized; besides, in a slightly acidic tumor microenvironment, imine (-NH-) protonation in PDA enhances adsorption of peroxymonosulfate through hydrogen-bond interaction, further promotes generation of. SO4 <->, finally causes mitochondrial dysfunction, effectively and synergistically enhances the chemotherapy effect of temozolomide on glioblastoma, and overcomes the drug resistance of glioblastoma.

The invention discloses a photo-activated myocardial targeting nano capsule for heart failure, and belongs to the technical field of biological medicines and nano materials. The nanometer capsule-like body comprises an inner core which is a nanometer capsule-like body unit, is obtained by nanocrystallization of a plant capsule-like body film and retains photoreaction activity; the shell is a bionic coating layer derived from a myocardial cell membrane. The invention also provides a preparation method of the nano capsule-like body and application of the nano capsule-like body in preparation of medicines for treating pressure load related cardiomyopathy (such as heart failure, cardiac hypertrophy and fibrosis). According to the nanometer cystoid, myocardial targeted enrichment is achieved through myocardial cell membrane coating, ATP is controllably generated under specific red light irradiation, myocardial cell energy metabolism defects are repaired, the mitochondrial function is improved, and therefore myocardial hypertrophy and fibrosis are effectively inhibited on cell and animal models, and the heart function is improved. The invention provides a novel energy metabolism repairing strategy which is triggered on demand and controllable in dosage for heart failure treatment.

The invention belongs to the technical field of biomedical materials, and particularly relates to a light / dark dual-mode intelligent antibacterial nano platform and a preparation method and application thereof. Aiming at the defect that photocatalytic antibiosis must depend on a light source to play a role, the invention constructs a light / dark dual-mode antibacterial nano platform which can generate free radicals and hydrogen peroxide under the irradiation of a xenon lamp and can efficiently kill pathogenic bacteria; and in the dark without a light source, the breathing of bacteria can be interfered through the action between the material and the bacteria, so that a certain sterilization effect is achieved, and the intelligent antibacterial nano material platform has great application potential. The preparation method of the novel therapeutic antibacterial material is mature and efficient, mild and controllable in condition and low in cost, and has high application value in the treatment aspect of overcoming bacterial drug resistance.

The invention relates to an IRE electrical sensitization nanometer material capable of regulating and controlling a tissue dielectric constant, a manganese carbonate nanometer material is manganese carbonate nanometer particles, the manganese carbonate nanometer particles are of a rhombohedral structure, and the average particle size is about 100-200 nm. According to the invention, the IRE ablation efficiency and tumor selectivity are obviously improved, and the residue and recurrence risk is reduced; economically, expensive equipment and complex processes are not needed, and the cost is controllable; in society, prognosis of patients is improved, life quality is improved, and safe and efficient treatment of solid tumors such as liver cancer is promoted.