42results about "Agression altered materials" patented technology

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

The invention relates to an ultrasonic response bionic piezoelectric nano-drug for treating epilepsy as well as a preparation method and application of the ultrasonic response bionic piezoelectric nano-drug. The ultrasonic response bionic piezoelectric nano-drug comprises hafnium-based piezoelectric nano-particles UIO-66-NH2 etched by sulfuric acid, an anti-epileptic drug, a microglial cell membrane and transferrin receptor targeting peptide, wherein the anti-epileptic drug is loaded in hafnium-based piezoelectric nanoparticles etched by sulfuric acid to form a drug loading core; the microcolloid cell membrane coats the outer surface of the drug loading core; the transferrin receptor targeting peptide is connected to a microglial cell membrane. The ultrasonic response bionic piezoelectric nano-drug is high in brain entering efficiency, stable in drug release amount and clear in preparation method path, is suitable for targeted delivery of various anti-epileptic drugs and treatment and research of nervous system diseases such as epilepsy, can adopt an intravenous injection administration mode and non-invasive administration, can avoid injury caused by an operation, and has a good application prospect. The electrical stimulation is generated in vivo without implanting and taking out the electrode, so that secondary injury and infection are avoided.

Methods for enhancing intracellular uptake of active agents by inducing temporary pore formation in a cell membrane are described. The methods generally comprise introducing nanoparticles to a cell in vivo, ex vivo, or in cell culture to magnetic nanoparticles that are taken up into the cell interior. The methods further comprise introducing active agents to the cell. A magnetic field is applied to the cell for targeted excitation of the internalized magnetic nanoparticles to induce temporary pore formation in the cell membrane, such that the active agent is taken up in an increased amount and / or at an increased rate by the cell.

The invention belongs to the technical field of medicines, and relates to injectable piezoelectric hydrogel for a spinal cord injury part as well as a preparation method and application of the injectable piezoelectric hydrogel. According to the hydrogel, methacrylated gelatin is used as a carrier, and a piezoelectric material MOF (at) PDA (at) M0, namely MP (at) M, of an enveloping porous structure is loaded in the hydrogel. The hydrogel can achieve dual functions under the ultrasonic action: (1) MP (at) M has piezoelectricity, ultrasonic mechanical energy is converted into electrical stimulation, calcium ion inward flow of astrocytes is triggered, mitochondria is promoted to be transferred from the astrocytes to neurons, and the mitochondrial function of the neurons is recovered; (2) the CD73 enzyme carried by the microglial cell membrane coated on the surface of the MP (at) M can degrade pro-inflammatory ATP (adenosine triphosphate) at the damaged part to generate adenosine, so that astrocytes are promoted to release lactic acid, and rapid energy support is provided for neurons. Mitochondrial transfer and energy supply are synchronously promoted through ultrasonic stimulation, neuronal plasticity is remodeled, spinal cord axon regeneration is promoted, and a new strategy is provided for spinal cord injury treatment.

The invention discloses a preparation method of a piezoelectric MOF (Metal Organic Framework) sound-sensitive agent with an ultrasonic response ROS / CO synergistic antibacterial function. The preparation method comprises the following steps: synthesizing a catechol modified ruthenium carbonyl carbon monoxide donor through a coupling reaction of 3, 4-dihydroxyl-1-phenylalanine and carbonyl ruthenium chloride; the method comprises the following steps: mixing an L-carnosine aqueous solution and a zinc ion aqueous solution in a solvent, and self-assembling the two solutions by a hydrothermal method to obtain a piezoelectric metal organic framework (PMOF); and modifying the carbon monoxide donor on the surface of the PMOF through a complexation reaction between zinc ions on the surface of the PMOF and phenolic hydroxyl groups of the carbon monoxide donor to obtain the piezoelectric MOF sound-sensitive agent with ultrasonic response ROS / CO synergistic antibiosis. The invention also discloses the piezoelectric MOF sonosensitizer with ultrasonic response ROS / CO synergistic antibacterial effect obtained by the preparation method and application of the piezoelectric MOF sonosensitizer in preparation of sonosensitizer hydrophobic drugs and / or antibacterial agents. The sonosensitizer provided by the invention has excellent biocompatibility, can controllably start release of active oxygen and carbon monoxide through ultrasound to achieve synergistic antibiosis, enhances the antibacterial ability, and has excellent biological membrane penetrating ability.

The present disclosure includes a system for treating cancer, the system comprising: an extracorporeal circulation device that is for collecting and returning the blood of a subject, and that includes a blood circuit and a blood pump; and a light irradiation device that is for irradiating the blood circuit with light. A photosensitive substance is administered to the subject before blood collection or is added, before light irradiation, to blood collected from the subject. The blood that has been collected from the subject and has been irradiated with light is returned to the subject. The irradiation energy density of the light is 2.5-300 J / cm2.

The invention relates to the technical field of biology, in particular to acoustoelectric Ba0. 85Ca0. 15Ti0. 9Yb0. 1O3 nanoparticles capable of triggering post-translational modification as well as a preparation method and application of the acoustoelectric Ba0. 85Ca0. 15Ti0. 9Yb0.1 O3 nanoparticles. Stirring and mixing the titanium tetrachloride solution and the mixed reagent solution to obtain a stirred mixed solution; mixing the stirred mixed solution with sodium hydroxide and polyvinylpyrrolidone, and performing heat treatment to obtain a heat-treated substance; and carrying out solid-liquid separation on the heat-treated substance to obtain a solid, washing the solid to be neutral, and drying. The nanoparticles provided by the invention consume H < + > near mitochondria under ultrasonic stimulation, so that mitochondrial membrane potential depolarization is caused. After mitochondrial membrane potential depolarization, E3 ubiquitin ligase Parkin is transferred from cytoplasm to a mitochondrial outer membrane, and is massively gathered and accumulated on the mitochondrial outer membrane, so that ubiquitination modification and cascade degradation of mitochondrial outer membrane anti-apoptosis protein MCL-1 are caused, and cell apoptosis is promoted.

The invention discloses a zinc oxide nanosheet and a preparation method and application thereof. The zinc oxide nanosheet is of an ultrathin sheet structure with the thickness of 3-6 nm and has piezoelectric catalytic activity. The preparation method comprises the following steps: dissolving zinc salt and hexamethylenetetramine in a high-volume-fraction ethanol water solution, and stirring at 55-65 DEG C for reaction. The nanosheet is applied to preparation of drugs for destroying bacterial biofilms or treating implant-related infections, and needs to be combined with ultrasonic irradiation. Under the ultrasonic action, the nanosheets generate a piezoelectric catalytic effect, lactic acid in a biological membrane microenvironment can be degraded, immunosuppression is relieved, and macrophages are promoted to be polarized to a pro-inflammatory phenotype; meanwhile, an ultrasonic cavitation effect is combined, so that a biological membrane can be synergistically and efficiently removed and bacteria can be killed.

The invention belongs to the technical field of biomedical materials, and particularly relates to ultrasonic-excited piezoelectric delivery exosome electrostatic spinning and a preparation method thereof.The piezoelectric delivery exosome electrostatic spinning comprises piezoelectric electrostatic spinning prepared by embedding barium titanate nanoparticles into polylactic acid, the surface of the piezoelectric electrostatic spinning is modified by chitosan and then loaded with exosome, and the surface of the piezoelectric electrostatic spinning is loaded with the exosome. The preparation method comprises the following steps: preparation of piezoelectric electrostatic spinning: dissolving polylactic acid in hexafluoroisopropanol, adding barium titanate nanoparticles, and carrying out ultrasonic dispersion to form a uniform precursor solution; piezoelectric electrostatic spinning containing barium titanate is prepared through high-voltage electrostatic spinning; piezoelectric electrostatic spinning surface modification: immersing the piezoelectric electrostatic spinning into a chitosan solution for incubation, and modifying the piezoelectric electrostatic spinning to form piezoelectric electrostatic spinning with positive electricity on the surface; the modified piezoelectric electrostatic spinning is soaked in an exosome suspension to be incubated, and piezoelectric exosome delivery electrostatic spinning is prepared and has the self-energy-supply function, the space-time response function and the biological delivery function, the defects of traditional biological medicine materials are hopefully overcome, and the piezoelectric exosome delivery electrostatic spinning can be applied to the field of biological medicine materials. The clinical transformation application of the intelligent biological material in the chronic wound repair of diabetes mellitus is promoted.

The invention discloses efficient tumor sonodynamic therapy nanoparticles based on carbon-doped nano-zinc oxide as well as a preparation method and application of the efficient tumor sonodynamic therapy nanoparticles. Belongs to the field of biomedicine. On the basis of MOF-5, carbon-doped hollow-hole-shaped nano-particles C-ZnO are formed after calcination in a nitrogen atmosphere, then chloroplatinic acid hexahydrate and sodium borohydride are added into a C-ZnO nano-particle solution, so that Pt nano-particles are loaded on the surface of C-ZnO, in addition, AB is loaded in a CZP hollow structure, HA-AA modification is carried out on the surface of the CZP hollow structure, and the CZP hollow structure loaded with the AB is obtained. Therefore, the effects of hydrogen treatment, consumption of excessive glutathione in a biological microenvironment and targeting are achieved, and the effect of treating cancers is achieved from multiple aspects. The nano particles are uniform in size, the average particle size is about 200 nm, good biocompatibility is achieved, and the material prepared through the method has excellent ROS generation capacity, so that the material has excellent sonodynamic therapy potential and has wide application prospects in the field of tumor therapy.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

Provided herein are compositions including biocompatible magnetizeable nanoparticles. The nanoparticles have a diameter (average diameter) from about 10 to about 300 nanometers and are biocompatible and magnetic. The nanoparticles may be a disc formed from iron oxide. The disc may be conjugated to a target-binding moiety capable of binding a target. The target may be cancer cells, pathogens, fat cells, or atherosclerotic plaques.

The invention provides a CO intelligent release platform based on a flexible implantable circuit and NFC induction. A forming method of the CO intelligent release platform comprises the following steps of constructing an NFC-LED module, forming a PDMS substrate, preparing a photosensitive composite layer, integrating devices and wirelessly activating a system. According to the CO intelligent release platform based on the flexible implantable circuit and NFC induction, a controllable CO release system based on a (carbon nitride / gold) composite nano-catalyst is constructed, and multiple mechanisms such as wireless NFC activation, blue light driving, nano-material directional reaction, release rate accurate regulation and control, targeted therapy and the like are combined; the defects of poor drug release accuracy, strong activation mode trauma, non-adjustable release rate, indefinite treatment window, high system energy consumption and the like in the prior art are overcome.

This invention related to manufactured microbubbles, as well as methods of using manufactured microbubbles, for example, in medicinal applications. The invention pertains to the physical structure and materials of the microbubbles, as well as to methods for manufacturing microbubbles, methods for targeting microbubbles for specific medicinal applications, and methods for delivering microbubbles in medical treatment.

Methods and systems for improving or enabling delivery of antibodies, immunoglobulin receptors, or immunotherapy agents across the Blood-Brain Barrier (BBB) are disclosed. Exosomes are derived from stem cells, immune cell progenitors, or immune cells, and are loaded with an antibody, immunoglobulin receptor, or antibody fragment. Focused energy, preferably low-intensity focused ultrasound, is applied to a region of the BBB of a patient, and loaded exosomes are administered to the patient. Exosomes target the region for delivery of the loaded antibody, receptor, or fragment, either by traversing the BBB at the region or releasing the loaded antibody to neural tissue at the region.

Method for the uptake / internalization of magnetic nanoparticles (MNPs) by immune cells, comprising the steps of preparing a mixture of a given amount of immune cells with a given amount of MNPs, and then exposing that mixture to an alternating magnetic field, at a given frequency and a given magnetic field strength, in order to heat that mixture to a temperature between 38° and 43° C, for a given time.

The application relates to the technical field of composite piezoelectric materials, and particularly discloses a composite nano piezoelectric material loaded with maslinic acid, a preparation method and application thereof, which comprises the following steps: adding barium titanate nano powder and maslinic acid into a solvent in sequence, performing ultrasonic treatment, then stirring and treating at 50-70 DEG C for 1.5-3h to obtain a mixture; adding PVDF powder into the mixture, stirring and treating at 50-70 DEG C for 4-6h to obtain a final mixed solution; meanwhile, washing and drying a glass slide to obtain a treated glass slide substrate; coating the mixed solution on the glass slide to form a film, drying to obtain a precursor film; and performing polarization treatment on the film to obtain the composite nano piezoelectric material loaded with maslinic acid. By introducing the barium titanate and the maslinic acid, the application effectively induces the PVDF to form a high-content polar beta crystal phase, thereby significantly improving the piezoelectric performance of the PVDF, and meanwhile, the flexibility of the PVDF film is also enhanced, the wearable patch substrate is suitable, and the application exhibits good drug controlled release potential.

The invention belongs to the technical field of biological pharmacy, and particularly relates to RVG29 modified dual-response drug-loaded nanoparticles as well as a preparation method and application thereof. According to the RVG29 modified dual-response drug-loading nano-particles, an organic metal framework with a piezoelectric property is taken as a carrier, eslicarbazepine acetate is loaded on the organic metal framework with the piezoelectric property, and the surface of the eslicarbazepine acetate is coated with a lipid layer to obtain the nano-particles; the maleimide group on the lipid layer and the sulfydryl on the targeting RVG29 polypeptide are subjected to a Michael addition reaction, and the targeting RVG29 polypeptide is covalently coupled to the surface of the nanoparticle, so that the RVG29 modified dual-response drug-loaded nanoparticle is obtained. The RVG29 modified dual-response drug-loaded nanoparticles are a safe and efficient anti-epilepsy strategy, and a non-invasive method not only provides a new thought for epilepsy treatment, but also provides an important reference for developing therapy of other nervous system diseases based on electrical stimulation.

To provide a method for improving therapeutic properties of stem cells.SOLUTION: In one aspect, a method of preconditioning stem cells comprising exposing stem cells to low dose radiation (LDR) is provided. In another aspect, a population of preconditioned stem cells is provided, where the population of five preconditioned stem cells is obtained by exposing stem cells to LDR. Uses of the preconditioned stem cells are also provided. In other aspects, the stem cells are muscle stem cells.SELECTED DRAWING: None

The application provides a bismuth-doped sodium niobate / oxygen vacancy ceria heterojunction and a preparation method and application thereof. The bismuth-doped sodium niobate / oxygen vacancy ceria heterojunction comprises the following raw material components in moles: 3-5 moles of bismuth-doped sodium niobate; and 1 mole of oxygen vacancy ceria. The bismuth-doped sodium niobate / oxygen vacancy ceria heterojunction has strong piezoelectric properties and acoustic power properties, and has enzyme-like activity. Under the driving of ultrasonic waves, the piezoelectric effect of the material not only promotes the proliferation of osteoblasts, but also enhances the acoustic power and enzyme-like activity of the material, generates various ROS, cooperatively kills bacteria and removes biofilms, and has potential application prospects in the treatment of infected bone tissues.

The invention relates to the field of medicine, in particular to a preparation method of energy-saving thermal therapy massage cream based on sea salt addition, which comprises the following steps: step S1, adding a heat storage mixture into a reaction kettle, heating to 40-60 DEG C, adding emulsifiers cetearyl alcohol ether-2 and cetearyl alcohol ether-21, stirring for 8-12 minutes at the rotating speed of 200-400 rpm to obtain an oil phase, and filtering to obtain a filtrate; by constructing a triple synergistic energy circulation system and combining a porous carrier staged controlled release technology and a low-temperature interface stabilization technology, self-supply and precise regulation and control of thermal therapy energy are achieved, dependence on an external heat source is reduced, the technical bottlenecks that a traditional thermal therapy product is large in temperature fluctuation, shallow in action level and poor in storage stability are broken through, and the thermal therapy effect is good. The continuous thermal therapy effect of deep tissues is realized while the mildness and comfort are ensured, and the product is suitable for the fields of exercise rehabilitation physical therapy and family health care, and has a wide market prospect.

Methods and systems for improving or enabling delivery of antibodies, immunoglobulin receptors, or immunotherapy agents across the Blood-Brain Barrier (BBB) are disclosed. Exosomes are derived from stem cells, immune cell progenitors, or immune cells, and are loaded with an antibody, immunoglobulin receptor, or antibody fragment. Focused energy, preferably low-intensity focused ultrasound, is applied to a region of the BBB of a patient, and loaded exosomes are administered to the patient. Exosomes target the region for delivery of the loaded antibody, receptor, or fragment, either by traversing the BBB at the region or releasing the loaded antibody to neural tissue at the region.

This invention relates to the field of peptide drugs, specifically to the field of ultrasound physical field-mediated regulation of biological signals and bone tissue repair technology, and more specifically to a piezoelectric hydrogel loaded with responsive peptides and its application. The application is based on a biological signal regulation method of ultrasound-induced responsive peptides LLPS and its application in promoting bone differentiation. It achieves spatiotemporal specific activation of biological signal pathways through precise regulation mediated by physical fields.

Disclosed herein is a method of promoting drug uptake via TRPC1 channels using a pulsed electromagnetic field (PEMF). Also disclosed herein are methods of treating cancer using PEMF adjuvant therapy and methods of selecting cancer patients for PEMF adjuvant therapy by detecting TRPC1 levels in a cancer sample.

The invention discloses a FeMOF / PtCu heterojunction sound-sensitive agent as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The sound-sensitive agent is endowed with piezoelectric property by constructing a heterojunction structure between FeMOF and PtCu, so that the sound-sensitive agent not only can efficiently catalyze hydrogen peroxide to generate active oxygen under the ultrasonic action, but also can generate oxygen through Pt catalysis, and meanwhile, cells are synergistically induced to generate ferroptosis and copper death through exogenous release of Fe < 2 + > and Cu < 2 + >; the preparation method comprises the steps of hydrothermal synthesis of FeMOF, thermal decomposition preparation of PtCu and compounding of FeMOF and PtCu through surface modification, and the prepared heterojunction sound-sensitive agent has good sonodynamic performance and metal ion release characteristics, shows remarkable active oxygen generation capacity and cytotoxicity to 4T1 cells under in-vitro conditions, and can be used for preparing the heterojunction sound-sensitive agent. And a novel multifunctional nano material for reference is provided for the field of sonodynamic therapy.

The invention provides an inner ear drug delivery method in a magnetic control targeting cochlea, and relates to the technical field of magnetic material application, and the method comprises the following steps: preparing a novel magnetic material carrier; the method comprises the following steps: after anesthetizing a rat, identifying occipital bone and positioning semicircular canals of the rat under an operating microscope, performing minimally invasive drilling by adopting a 1mL injector, and injecting a novel magnetic material carrier loaded with a target inner ear medicine into the semicircular canals of the rat; when the rat semicircular canal administration meets a preset time threshold value, the rat is placed in a 9.4 T magnetic resonance magnetic field, the magnetic field direction is from the bottom of the cochlea to the top of the cochlea, the target inner ear medicine is driven to move in the cochlea lymph, and target area release is executed. According to the invention, the technical problem that the medicine is difficult to uniformly and accurately distribute in the inner ear due to the fact that the medicine can only passively move along with cochlea lymph fluid and cannot be administrated to a specific part in the cochlea in a traditional inner ear local administration method is solved.

This invention relates to the field of biomedical technology, and more particularly to the application of low-intensity pulsed ultrasound in promoting the uptake of exosomes carrying chemotherapeutic drugs by tumor cells. The invention uses an intensity of 200 mW / cm². 2 ~350mW / cm 2 Low-intensity pulsed ultrasound, without damaging exosomes carrying chemotherapeutic drugs, promotes the uptake efficiency of these drugs by tumor cells, thereby enhancing the therapeutic effect of chemotherapeutic drugs on tumor cells. This invention, through cell and animal experiments, demonstrates that low-intensity pulsed ultrasound combined with exosomes carrying chemotherapeutic drugs can enhance the therapeutic effect of chemotherapeutic drugs on tumor cells, providing new theoretical and experimental basis for tumor treatment and showing promising application prospects.

The invention provides a targeted analgesic microsphere based on a piezoelectric material as well as a preparation method and application of the targeted analgesic microsphere. The method comprises the following steps: grafting rabies virus glycoprotein 29 peptide onto barium titanate particles, preparing a BaTiO3-RVG solution, then putting composite hydrogel prepared from GelMA and HAMA into the BaTiO3-RVG solution, and carrying out vacuum freeze-drying, so as to obtain the analgesic microspheres. The obtained analgesic microspheres can significantly reduce sodium ion inflow in DRG cells and expression of Nav1.7 channels and pain-related molecules, show a good analgesic effect, can inhibit pain signal conduction by regulating and controlling voltage-gated sodium channels, and provide a new thought for osteoarthritis pain treatment.

The invention relates to the field of polypeptide medicines, in particular to the technical field of regulation and control of biological signals and bone tissue repair by an ultrasonic physical field, and particularly relates to piezoelectric hydrogel loaded with response polypeptide and application thereof, and the application is a biological signal regulation and control method based on ultrasonic induced response polypeptide LLPS and application of promoting bone differentiation. Through precise regulation and control mediated by a physical field, space-time specific activation of a biological signal channel is realized.

The application relates to the technical field of biology, and particularly relates to a sound electricity Ba that triggers post-translational modification 0.85 Ca 0.15 Ti 0.9 Yb 0.1 O3 nanoparticles, a preparation method and application thereof. A titanium tetrachloride solution and a mixed reagent solution are stirred and mixed to obtain a stirring mixed solution; the stirring mixed solution is mixed with sodium hydroxide and polyvinylpyrrolidone and then subjected to heat treatment to obtain a heat-treated product; the heat-treated product is subjected to solid-liquid separation to obtain a solid, and the solid is washed to be neutral and dried. + The nanoparticles provided by the application consume H + around mitochondria under ultrasonic stimulation, leading to depolarization of a mitochondrial membrane potential. After the mitochondrial membrane potential is depolarized, E3 ubiquitin ligase Parkin is transferred from a cytoplasm to a mitochondrial outer membrane, and is massively aggregated and accumulated on the mitochondrial outer membrane, leading to ubiquitination modification and cascade degradation of an anti-apoptotic protein MCL-1 of the mitochondrial outer membrane, so that cell apoptosis is promoted.