30 results about "Sonoporation" patented technology

The present invention is directed to devices and methods that apply ultrasonic energy for the purpose of inducing transfection and cell transformation. A sonoporation system in accordance with embodiments of the present invention includes an ultrasonic electrical energy generator connected to an ultrasonic transducer producing stress waves. The ultrasonic transducer is connected to a fluid containment tank configured to accept at least a portion of the ultrasonic transducer whereby the ultrasonic stress waves may be delivered into the fluid medium. A cell holder is configured to hold one or more cells desirable for transfection. A hydrophone may be electrically connected to an acoustic stress wave intensity detection circuit. A motion control system having an arm configured to receive one or both of the cell holder and the hydrophone is configured to provide motion of one or both of the cell holder and the hydrophone within the fluid medium.

The invention relates generally to systems and devices for material delivery, energy delivery, and / or monitoring electrophysiological activity, and method of use thereof. In particular, the present invention provides devices and systems, and methods of use thereof, configured to deliver therapeutic compositions, to provide electroporation and / or sonoporation to increase therapeutic efficiency, and to monitor electrophysiological activity, for example, before and after treatment.

Medical apparatus and processes controllably generate medically useful micro or nano bubbles of medically desirable and controllably selectable size, size distribution, homogeneity and concentration (and / or other key bubble parameters) for patient infusion, and / or which may incorporate therapeutic or other agents for patient infusion and / or may be combined with therapeutic or other agents prior to infusion into patients. The bubble generation apparatus and processes controllably permit the adjustment and selection of key bubble parameters through bubble generation actuation and orientation techniques and through selection of bubble fluid compositions in order to facilitate medical research and / or to optimize treatment for imaging, therapy, sonoporation, inertial and non-inertial cavitation, and acoustic activation, among other medical uses. Disposable cartridges containing such bubbles are provided and may include means for patient infusion.

The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.

A method, using high intensity ultrasound, which may also be combined with a chemotherapy agent, that can result the direct destruction of tumor cells and in the reduction or elimination of local reoccurrence of cancer after removal of cancerous tissue, such as a surgical breast lumpectomy or surgical excision of a brain tumor. The method comprises either (1) the treatment of the tumor directly with High Intensity Focused Ultrasound, or (2) the treatment of the margins of the tissue surrounding the surgical cavity or void with ablative continuous wave high intensity ultrasound and a combination of a locally delivered chemotherapy agent and high intensity ultrasound, termed sonoporation. The present invention permits both the direct destruction (ablation) of tumor tissue as well as the destruction of tissue around the surgical margin and may include the enhanced local cellular uptake of locally injected chemotherapeutic drugs, all of which can be accomplished by a therapeutic ultrasound device used during surgery.

An apparatus for performing in-vivo sonoporation of a skin area and transdermal and / or intradermal delivery of a drug solution includes a container having an end adjacent the skin area and containing the drug solution. The container further includes an ultrasound horn having a tip submerged in the drug solution for applying ultrasound radiation to the drug solution. The ultrasound radiation has a frequency in the range of 15 KHz and 1 MHz and is applied at an intensity, for a period of time and at a distance from said skin area effective to generate cavitation bubbles. The cavitation bubbles collapse and transfer their energy into the skin area, thus causing the formation of pores in the skin area. The ultrasound radiation intensity and distance from the skin area are also effective in generating ultrasonic jets, which ultrasonic jets then drive the drug solution through the end adjacent the skin area and the formed pores into the skin.

The invention relates to an ultrasonic treatment device and a method mediated by microbubbles for opening prostate barrier. The ultrasonic treatment device comprises an ultrasonic treatment unit and microbubbles, and the ultrasonic treatment device is characterized in that direct-current high voltage is used to generate high pulse, a high pulse switch functional tube is used as a control unit, the direct-current high voltage is supplied to an ultrasonic power oscillator by means of high pulse, and pulse signals are sent out. The treatment unit is capable of transmitting an intermittent pulse type ultrasonic energy form low in average sound intensity and high in peak sound pressure. In coordination with intravenous microbubble injection, microbubble ultrasonic cavitation in blood circulation is induced under pulse type ultrasonic transmission, the prostate barrier is opened noninvasively under microbubble sonoporation, drug penetration into prostate tissue cells is promoted, a novel way of noninvasive prostate drug treatment is developed, drug effect is given to fullest play, drug application time is shortened, and treatment cost is saved.

The invention provides a sound production device, a display system and a sound production method of the sound production device, belongs to the technical field of display, and can solve the problems that the sound production orientation effect of the existing sound production device is poor and the sound production orientation cannot be automatically changed along with the change of a target direction. The sound production device comprises a plurality of ultrasonic transducers, the ultrasonic transducers can form at least one sound production unit, and each sound production unit comprises twoultrasonic transducers; One ultrasonic transducer in each sound production unit is used for transmitting first ultrasonic waves, and the other ultrasonic transducer in each sound production unit is used for transmitting second ultrasonic waves; The sound production control unit is configured to determine a target direction and output a direction instruction to the motion unit according to the target direction; And the motion unit is configured to adjust the sound production direction of the ultrasonic transducers according to the direction instruction, so that the two ultrasonic transducers ofeach sound production unit generate sound towards the target direction and then generate difference frequency sound waves in an audible frequency range in the target area.

Ultrasound mediated delivery (USMD), real-time quantitative feedback derived (S264) therefrom, and proceeding by the system based on the feedback all are, in some embodiments, operable automatically and without need for user intervention. USMD may occur in a clinical setting accompanied by assays (S276) or real-time feedback, or by means of a wearable device that, based on feedback, regulates USMD in real time. Optionally, the user is provided an indication (S281) as to progress or success, of a treatment. Electrodes (128) may be attached across tissue in which transient pores are produced via sonoporation in the USMD procedure, and in vivo measurement is taken of an electrical parameter responsive to permeability. Therapeutic agent (S202) may be administered after particles activated for sonoporation are cleared from the circulation, to avoid, when it might exist, adverse interaction between the particles and agent.

A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Systems and methods for treating tissue by directing light pulses using bubbles generating in tissue using previously transmitted light pulses are disclosed. Systems and methods for treating tissue using a lens array comprising a pitch or separation distance sized to overlap sonoporation induced shockwaves are also disclosed. In one embodiment, the shockwaves are generated in response to incident light pulses directed through adjacent lenses in the array. Systems and methods can improve porosity of the cellular membrane. Systems and methods for creating channels in tissue by using stacked pulses are also disclosed.

The invention discloses a cluster acoustic wave precipitation enhancement device, comprising an acoustic wave sound generator (5), an acoustic wave transducer (4), a cylindrical box cover (2), a matrix type energy-gathering trumpet (3), and an energy-gathering cylindrical acoustic wave cover (1), wherein the acoustic wave sound generator (5) generates certain high-frequency electric energy supplied to the acoustic wave transducer (4), then the electric energy is transmitted to the energy-gathering cylindrical acoustic wave cover (1) by using the directional matrix type energy-gathering trumpet (3), and a nonlinear acoustic effect of air is generated in the energy-gathering cylindrical acoustic wave cover (1), so that controllable artificial precipitation is achieved. The cluster acoustic wave precipitation enhancement device disclosed by the invention is environmentally friendly and free from pollution free, strong in directivity, small in airspace restriction, and high in preparatory work and operation efficiencies.

The invention relates to an agitating type bioreactor for culturing microorganisms by sound waves. The bioreactor comprises a tank and an agitating device; the inner wall of the tank is provided with a sound generating mechanism; the sound generating mechanism comprises 4 signal generating units and 8 groups of sound generating rods arranged on the inner wall of the tank; each group of sound generating rods comprises an ultrasound generating rod and an audible sound generating rod; the 8 groups of sound generating rods are arranged in suitable positions on the inner wall of the tank; each signal generating unit is connected with two groups of sound generating rods through cables; the signal generating units generate high-frequency signals through MAX038-function sound generators, and the high-frequency signals drive the sound generating rods to generate sound after being amplified; the 8 groups of sound generating rods are arranged in different positions on the inner wall of the tank and can generate audible sound waves and ultrasound waves simultaneously, and the sound waves coordinate mutually to stimulate and culture the microorganisms in the tank so as to achieve an unexpected expect and greatly improve the microorganism fermentation efficiency. The bioreactor provided by the invention has good industrial application prospects.

System for inducing sonoporation of a drug into cancer cells in a tumor and method thereof, the system comprising a generator configured to provide electrical energy at an ultrasound frequency; an ultrasound probe electrically connected to the generator and configured to convert the electrical energy into low intensity pulsed ultrasonic waves defined by operation parameters, said operation parameters comprising the frequency, the duty cycle, the operation time of the ultrasonic waves; an input device enabling an operator to enter configuration data comprising: type of tumor, type of drug, localization of secondary tumor, anthropometric measurements and grade of tumor, and a processor configured to determine the values of the operation parameters on the basis of the entered configuration data and control the generator and the ultrasound probe to operate according to said determined values, wherein the value of the frequency is determined on the basis of the type of tumor, the localization of the tumor, the grade of tumor and the anthropometric measurements, the value of the duty cycle is determined on the basis of the drug, the type of tumor and the grade of the tumor, and the value of said operation time being determined on the basis of at least the type of tumor and the type of drug.

The invention relates to a cleaning machine which comprises a water tank body with a washing cavity, the water tank body is made of plastic, the cleaning machine further comprises a metal part, the metal part is arranged on the inner wall face of the upper portion of the water tank body in the circumferential direction, and reinforcing ribs are arranged on the outer circumferential wall of the water tank body. The water tank body is made of plastic, the plasticity is high, the depth of the tank body can be increased conveniently according to needs, and the production cost is greatly reduced; the metal part and the reinforcing ribs can prevent the water tank body from being heated or cooled to deform. Meanwhile, the metal part can further resonate with an ultrasonic sound production deviceat the bottom of the water tank cleaning machine, and the ultrasonic cleaning effect is improved. According to the ultrasonic water tank, the reinforcing ribs are arranged on the outer wall face of the water tank body, on one hand, the reinforcing ribs can prevent the water tank body from being heated or cooled to deform, and on the other hand, when the ultrasonic sound production device is started by the water tank body, the reinforcing ribs on the lower portion of the water tank body facilitate transmission of ultrasonic waves and improve the ultrasonic cleaning effect.

The invention discloses a high-speed homogenizer with a sound wave coordinated dispersion and temperature control structure. The high-speed homogenizer comprises an ultrasonic sound production mechanism, a high-speed homogenizing mechanism, a low-temperature constant-temperature mechanism and a control panel, the ultrasonic sound production mechanism is installed in the upper end of the low-temperature constant-temperature mechanism, one end of the high-speed homogenizing mechanism is installed at the upper end of the low-temperature constant-temperature mechanism, the other end of the high-speed homogenizing mechanism is inserted into the ultrasonic sound production mechanism, and the control panel is installed at the upper end of the low-temperature constant-temperature mechanism and electrically connected with the ultrasonic sound production mechanism, the high-speed homogenizing mechanism and the low-temperature constant-temperature mechanism. The high-speed homogenizer has the beneficial effects that the rotation of the high-speed homogenizing mechanism is matched with the staged starting and stopping of the driving piece, the vibration rod can be in a vibration state all the time, the vibration frequency of the vibration shell is larger than that of liquid without the vibration rod, so that the sizes of bubbles in water are reduced, the effect of eliminating the bubbles by ultrasonic waves generated by the ultrasonic sound production mechanism is improved, and component stability is facilitated.

The invention relates to a cleaning machine which comprises a water tank body with a washing cavity, at least the position, making contact with an installation plane, of an opening of the water tank body is made of metal, the position, below the opening, of the water tank body is made of plastic, and reinforcing ribs are arranged on the peripheral wall of the water tank body. The water tank body is made of plastic, the plasticity is high, the depth of the tank body can be increased conveniently according to needs, and meanwhile the production cost is greatly reduced; at least the position, making contact with the mounting plane, of the opening part of the water tank body is made of metal, the problem that the plastic water tank body is small in bearing force is solved, and the mounting stability of the water tank body is improved; according to the ultrasonic water tank, the reinforcing ribs are arranged on the outer wall face of the water tank body, on one hand, the reinforcing ribs can prevent the water tank body from being heated or cooled to deform, and when the ultrasonic sound production device is started by the water tank body, the reinforcing ribs on the lower portion of thewater tank body facilitate transmission of ultrasonic waves, and the ultrasonic cleaning effect is improved.

The invention discloses an ultrasonic sound generator, a display and electronic equipment, the ultrasonic sound generator comprises a vibrating body and a sound production driver, the sound production driver comprises a conductive circuit and a magnetic circuit unit, and the magnetic circuit unit comprises a plurality of magnetic steels which are distributed at certain intervals and arranged in multiple rows. Magnetic poles of two corresponding ends of two adjacent pieces of magnetic steel are opposite, the conductive circuit is attached to the vibrating body, a vibrating space is arranged between the vibrating body and the magnetic circuit unit, the conductive circuit is wired in a gap formed between the magnetic steel, and the wiring mode of the conductive circuit in the gap enables the conductive circuit to pass through the vibrating space after an audio modulated ultrasonic signal is loaded on the conductive circuit. And the sounding driver is driven to generate vibration perpendicular to the plane where the vibrator is located, so that the vibrator is driven to vibrate and sound. According to the invention, by adjusting the wiring structure of the conductive circuit, the size of the gap between the magnetic steels, the driving frequency and the like, segmentation vibration can be reduced while high directivity of screen sound production is realized.

The invention discloses an ultrasonic sound generator, a display and an electronic device, the ultrasonic sound generator comprises a vibrating body and a sound production driver, the sound production driver comprises a plurality of vibrating units arranged on the vibrating body, the plurality of vibrating units are arranged on the vibrating body in an array, an air gap is arranged between each vibrating unit and the vibrating body, and the air gap is arranged between each vibrating unit and the vibrating body. The distance between every two adjacent vibration units ranges from 2 mm to 100 mm, and the size of each vibration unit ranges from 1 mm to 100 mm. The sound production driver loads an ultrasonic signal, generates vibration perpendicular to the direction of the vibration body, and drives the vibrator to vibrate and produce sound. By adjusting the arrangement mode of the vibration units of the sound production driver, the size of the vibration units, the distance between the vibration units, the frequency of the ultrasonic signal and the like, sound distortion caused by segmentation vibration is avoided, the overall vibration amplitude of the sound production device can be reduced, the reliability of the whole sound production device is improved, and the sound production device is not prone to being broken or stripped.

The invention relates generally to systems and devices for material delivery, energy delivery, and / or monitoring electrophysiological activity, and method of use thereof. In particular, the present invention provides devices and systems, and methods of use thereof, configured to deliver therapeutic compositions, to provide electroporation and / or sonoporation to increase therapeutic efficiency, and to monitor electrophysiological activity, for example, before and after treatment.

The invention discloses a method for measuring flue gas temperature based on sound waves. The method comprises the following steps: S1, symmetrically mounting a pair of sound wave sounding devices on two side walls of a hearth to be measured on the same plane; s2, the embedded control and detection module controls an electromagnetic valve to be opened; s3, transmitting the sound wave signal to an embedded control and detection module; and S4, the embedded control and detection module transmits the collected signal data to the TSN gateway, and then transmits the signal data to the data processing and analysis platform through the OPCUA network for calculation, and the formula is as follows: the flow velocity of the flue gas is obtained through the propagation velocity difference of sound waves in two directions of the same path. And the three-dimensional flow field of the flue gas space is calculated by using the sound wave velocity difference in two directions of multiple paths, so that the system can continuously operate in a severe environment for a long time, and the reliability of the temperature measurement system is ensured.

The invention belongs to the technical field of pipeline detection, and discloses a real-time tracking and positioning system and method for a detector in a pipeline. The system comprises an inner detector, an infrasonic wave sounder, an infrasonic wave sounding controller, a signal collection device, and a monitoring terminal. The infrasonic wave sounder and the infrasonic wave sounding controller are both arranged in the inner detector, and the infrasonic wave sounding controller is in communication connection with the monitoring terminal and controls the infrasonic wave sounder to emit infrasonic waves. The signal collection device is in signal connection with the infrasonic wave sounder and the monitoring terminal and is used for collecting infrasonic wave signals sent by the infrasonic wave sounder and uploading the infrasonic wave signals to the monitoring terminal. According to the real-time tracking and positioning system and method for the detector in the pipeline, the real-time operation state and the specific position of the inner detector can be obtained for real-time positioning and tracking, and monitoring personnel can take measures in time according to the operation state and the specific position information of the inner detector, so that safety accidents are avoided.

The invention discloses double-combination magnetic resonance purification equipment, and belongs to the technical field of sewage treatment equipment. The equipment comprises a work box, a magnetic plate device, a polar plate device, an ultrasonic sound production device, a filtering device, a gas collection device and a flow control device, wherein the work box is placed in sewage, the magneticplate device is arranged on the work box, and the polar plate device is arranged on the side wall of the work box; the ultrasonic sound production device is mounted on the magnetic plate device, the filtering device is mounted in the work box, the gas collection device is mounted at the top of the work box, and the flow control device is mounted on the side wall of the work box; the magnetic platedevice promotes electrolytic efficiency, the polar plate device performs electrolytic purification on substances in sewage, the ultrasonic sound production device thoroughly crushes the substances inthe electrolytic sewage, the filtering device filters the substances subjected to electrolytic purification, the gas collection device filters gas, and the flow control device controls outflow of purified water.