The invention discloses a lomefloxacin hydrochloride injection and a preparation method thereof. The lomefloxacin hydrochloride injection is prepared from lomefloxacin hydrochloride, salt-forming agents, cosolvents, stabilizing agents, adsorbing agents and water for injection according to a specific proportion. The preparation method includes 1), weighing all the raw materials according to the specific proportion, and preparing a specific concentration of salt-forming agent solution from the salt-forming agents by the water for injection; 2), adding the lomefloxacin hydrochloride into the residual water for injection, then adding the salt-forming agent solution, continuously stirring till the lomefloxacin hydrochloride dissolves completely, adding the cosolvents and the stabilizing agents, uniformly stirring to reach a pH (potential of hydrogen) value of 10.2-10.4, adding the adsorbing agents, standing for 28-32 minutes after uniform stirring, filtering, filling, filling nitrogen, sealing and sterilizing at the temperature of 113-117 DEG C for 25-35 minutes. The lomefloxacin hydrochloride injection has the advantages of convenience and rapidity in administration, high stability, small preparation volume, convenience in transporting and low cost. The preparation method is simple in technological process, high in operability and applicable to large-batch industrial production.