The invention relates to a complete pellet feed for tree shrews and a preparation method thereof. The complete pellet feed comprises the following raw material components in percentage by weight: 38 to 40 percent of corn, 17 percent of barley, 10 percent of rice, 17 percent of bean pulp, 8 percent of fish meal, 2 percent of alfalfa meal, and 1.2 percent of egg powder which are taken as main raw materials, and 1 percent of sucrose, 0.5 percent of fructose, 1.5 percent of beef tallow, 1 to 1.5 percent of lard, 0 to 1.5 percent of whole milk powder, and 0.8 percent of amino acid, vitamin and trace element which are taken as additives. The raw materials are prepared into the pellet feed which has the diameter of 0.5cm and the length of 0.5 to 1.2cm. The pellet feed of the invention has overall and balanced nutrition, so that the stress reaction of the tree shrews caused by feed factor is avoided. The feeding is convenient, the requirements on the normal growing development of the tree shrews under laboratory conditions can be met, and the normal oestrus, mating, impregnation and lactation of the tree shrews all the year round are ensured.