Novel fixing device for mouse brain imaging

Abstract

The invention provides a novel fixing device for mouse brain imaging. The novel fixing device comprises a first fixing assembly, the bottom of the first fixing assembly is connected with an adjusting platform, the bottom of the adjusting platform is connected with a mounting bottom plate, a second fixing assembly is arranged on the mounting bottom plate, and one end of the second fixing assembly is close to the first fixing assembly; the first fixing assembly comprises an inverted-T-shaped plate, the inverted-T-shaped plate is installed at the top of the adjusting platform, two supporting cylinders are symmetrically arranged at the positions, close to the second fixing assembly, of the inverted-T-shaped plate, the top of each supporting cylinder is movably connected with a first connecting plate, a brain fixing piece is connected between the two first connecting plates, and the brain fixing piece is fixed to the head of a mouse. A positioning hole is formed in the brain fixing piece and used for positioning a window area for mouse brain imaging. According to the device, the stress reaction of the mouse in the experiment process is relieved, the reliability and repeatability of the experiment are improved, the absolute stability of the mouse in a waking state is ensured, and the accuracy of experiment data is effectively improved.

Description

Technical Field

[0001] The invention belongs to the technical field of experimental instruments, and in particular to a novel fixing device for mouse brain imaging. Background Art

[0002] The development and progress of imaging technology has provided favorable technical conditions for people to conduct brain science research. Since the brain structure of mice is highly similar to that of humans, the research on the brain of mice can be extended to the research on human brain science. In previous studies, most of them were brain imaging of mice under anesthesia, and few brain imaging studies were conducted on awake mice. Brain imaging of awake mice is of great significance to brain science research. It can help study the working mechanism of the brain and neural activity, reveal the connection and interaction between different brain regions, and help diagnose and evaluate diseases. The main reason for hindering research is that the skull of the mouse brain is small and it is difficult to fix the head and body. Existing fixing device structures, such as brackets, clamps, connecting rods, slide rails, etc., generally use high-precision mechanical parts and materials, and in order to ensure tight installation, precision processing is required, resulting in high manufacturing costs of the device; and existing mouse fixing devices use slide rails, spiral adjustment rods and connecting rods to adjust the operating space, and the adjustment space is limited. The limited adjustment space leads to poor adaptability and cannot ensure the fixation of mice of different sizes. The fixation effect is limited, which leads to the inability to ensure the stability of the mouse body and brain imaging area in the awake state, so that the collected data is affected by motion artifacts, hindering brain science research. Summary of the invention

[0003] In order to solve the problem that the fixing bracket used in the existing experiments cannot stably fix the head of the mouse in the awake state, the present invention provides a new fixing device for mouse brain imaging.

[0004] To achieve the above object, the present invention provides the following technical solutions: The present invention proposes a novel fixing device for mouse brain imaging, comprising a first fixing component for fixing the mouse head, the bottom of the first fixing component is connected to an adjustment platform for adjusting the tilt angle and position, the bottom of the adjustment platform is connected to a mounting base plate, a second fixing component for fixing the mouse body is arranged on one side of the adjustment platform on the mounting base plate, and one end of the second fixing component is close to the first fixing component; The first fixing component includes an L-shaped plate, which is installed on the top of the adjustment platform. Two supporting cylinders are symmetrically arranged on the L-shaped plate near the second fixing component. The top of each supporting cylinder is movably connected with a first connecting plate. A brain fixing plate is connected between the two first connecting plates. The brain fixing plate is fixed to the mouse head. A positioning hole is set on the brain fixing plate, and the positioning hole is used to locate the window area for imaging the mouse brain.

[0005] Preferably, a second connecting groove is provided on the brain fixing plate near its end, and a first adjusting groove is provided on the first connecting plate at a position corresponding to the end of the brain fixing plate, and the first adjusting groove and the second connecting groove are connected by a connecting screw.

[0006] Preferably, a second adjustment groove is provided on the end of the first connecting plate away from the first adjustment groove, a first fixing screw is installed in the second adjustment groove, and the bottom end of the first fixing screw is fixed to the top of the supporting cylinder.

[0007] Preferably, a third adjustment groove is provided on the end of the L-shaped plate close to the second fixing component, a second fixing screw is connected in the third adjustment groove, and the top end of the second fixing screw is fixed to the top of the supporting cylinder.

[0008] Preferably, the adjustment platform comprises a mounting platform, the mounting platform is fixedly mounted on the mounting base plate, a first adjustment platform is mounted on the top of the mounting platform, a first angle adjustment member is arranged between the mounting platform and the first adjustment platform, a first adjustment knob is connected to the first angle adjustment member, and the first adjustment knob is rotatably mounted on the mounting platform; A third adjustment platform is installed on the top of the first adjustment platform, a second angle adjustment member is arranged between the third adjustment platform and the first adjustment platform, a second adjustment knob is connected to the second angle adjustment member, and the second adjustment knob is rotatably installed on the first adjustment platform; A second adjustment platform is installed on the top of the third adjustment platform, a height adjustment member is arranged between the second adjustment platform and the third adjustment platform, a third adjustment knob is connected to the height adjustment member, and the third adjustment knob is installed on the third adjustment platform.

[0009] Preferably, the second fixing assembly comprises a support plate for placing the mouse body, a plurality of groups of fixing components are installed on the upper end of the support plate, each group of fixing components comprises two connecting platforms, the mouse body is located between the two connecting platforms in each group, wherein one connecting platform in each group of fixing components is provided with a slide groove, one end of a sliding pressing plate is connected to the other connecting platform, the other end of the sliding pressing plate passes through the slide groove and is fixed by the fixing rod, so that the sliding pressing plate fixes the mouse body; A first adjustment component is connected to the side end surface of the support plate opposite to the connecting platform, and the adjustment component is installed on the installation base plate.

[0010] Preferably, the support plate is symmetrically provided with sliding grooves, the connecting platform is installed in the sliding grooves, the connecting platform is provided with a fifth fixing hole, a fixing rod is installed in the fifth fixing hole, and one end of the fixing rod is closely attached to the mounting base plate; A third fixing member is arranged on the support plate near the end of the brain fixing plate, and the third fixing member includes a first fixing groove and a second fixing groove. The first fixing groove is used to insert the lower jaw of the mouse after the mouse's mouth is opened, and the second fixing groove is used to clamp the mouse's incisors.

[0011] Preferably, the first adjustment assembly includes an L-shaped bracket, the L-shaped bracket is installed on the mounting base, one side of the L-shaped bracket is connected to a connecting piece, the connecting piece is connected to a second connecting plate, the second connecting plate is connected to a third connecting plate, and the third connecting plate is fixed to the side end surface of the support plate.

[0012] Preferably, a plurality of adjustment holes are provided on the mounting base plate, and the L-shaped bracket is connected to the corresponding adjustment holes via a threaded rod.

[0013] Preferably, a heating pad is provided on the support plate.

[0014] Compared with the prior art, the present invention has the following beneficial technical effects: The present invention provides a novel fixing device for imaging the mouse brain. In the fixing device, a stable supporting foundation is provided by a first fixing component of the L-shaped plate, and the supporting cylinder and the first connecting plate thereon are combined so that the brain fixing plate can flexibly adapt to the shapes and sizes of different mouse heads, thereby ensuring the stability and accuracy of the mouse head during the imaging process. At the same time, the positioning holes arranged on the brain fixing plate accurately locate the window area for imaging the mouse brain, thereby ensuring that the imaging window is in the best position, providing great flexibility for the experiment, greatly improving the resolution and clarity of the imaging, and helping researchers to observe and analyze the structure and function of the mouse brain more accurately. The adjusting platform allows the entire device to be used in a variety of ways. The tilt angle and position can be fine-tuned according to experimental needs during the process, which not only helps to optimize the imaging angle and improve the imaging quality, but also provides researchers with more experimental flexibility and facilitates multi-angle and multi-level brain imaging research. The second fixation component effectively fixes the mouse's body, avoiding interference caused by the movement of the mouse's body during the imaging process, ensuring the stability of the imaging, helping to reduce the stress response of the mouse during the experiment, improving the reliability and repeatability of the experiment, and ensuring the absolute stability of the mouse in the awake state, effectively improving the accuracy of the experimental data, and reducing the impact of external factors on the experimental results, allowing more complex brain function and blood flow dynamics and other related brain science research.

[0015] Furthermore, the combination of the third adjustment groove and the second fixing screw provided on the L-shaped plate in the fixing device provides another flexible adjustment method for fixing the support cylinder, thereby enhancing the stability of the support cylinder so that it can better bear the weight of the mouse head, and by adjusting the position of the second fixing screw in the third adjustment groove, the height and inclination angle of the support cylinder can be further fine-tuned, thereby meeting the precise requirements for the fixed position of the mouse head under different experimental conditions, which helps to improve the accuracy and reliability of imaging experiments.

[0016] Furthermore, the adjustment platform in the fixing device can adjust the tilt angle and position to meet the needs of accurately adjusting the position and angle of the mouse in the experiment, and adapt to the needs of other experimental equipment, so that it can ensure that specific brain areas are within the observable range, improve experimental efficiency, and perform accurate observation or imaging. Moreover, through reasonable fixation and adjustment, the motion artifacts of awake mice during the experiment can be effectively reduced, ensuring that the images and data collected in the awake state are accurate, and helping to reduce the stress response of mice, improve their comfort, and help improve the reliability of experimental data.

[0017] Furthermore, in the fixation device, the second connecting groove on the brain fixation plate is connected to the first adjustment groove on the first connecting plate through a connecting screw, so that the brain fixation plate can be flexibly adjusted as needed to adapt to the head sizes and shapes of different mice. The combination of the second adjustment groove on the first connecting plate and the first fixing screw provides an additional fixing point for the brain fixation plate, enhances the stability and reliability of the fixation, thereby ensuring the absolute stillness of the mouse head during imaging and improving the imaging quality.

[0018] Furthermore, the third fixing member arranged on the support plate in the fixing device, the third fixing member includes a first fixing groove and a second fixing groove, which provide an additional fixing method for the mouse's head. The first fixing groove can accommodate the open lower jaw of the mouse, and the second fixing groove is used to clamp the mouse's incisors, effectively preventing the mouse's head from moving during imaging, further improving the stability and clarity of imaging, and ensuring the accuracy and reliability of experimental data.

[0019] Furthermore, the fixation device provides a stable support for the fixation of the mouse body through the combination of the L-shaped bracket, the connecting piece, the second connecting plate and the third connecting plate, and realizes the flexible adjustment of the position of the support plate, thereby enhancing the overall stability of the device, allowing researchers to easily adjust the position of the mouse body as needed, thereby optimizing the imaging effect and improving the accuracy and reliability of the experiment.

[0020] Furthermore, in the fixing device, by installing multiple adjustment holes set on the base plate and connecting the L-shaped bracket and the adjustment holes through threaded rods, the position of the second fixing component can be flexibly adjusted, thereby meeting the precise control of the fixed position of the mouse body under different experimental requirements, enhancing the versatility and flexibility of the device, providing researchers with more experimental options and operational convenience, and helping to improve the accuracy and efficiency of the experiment.

[0021] Furthermore, in the fixing device, a heating pad is arranged on the support plate to ensure that the mice maintain normal vital signs during the experiment, provide a comfortable imaging environment for the mice, and reduce imaging interference caused by temperature discomfort. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the structure of a novel fixation device for mouse brain imaging proposed by the present invention; Figure 2 This is a schematic diagram of the connection between the first fixing component and the moving platform in a novel fixing device for mouse brain imaging proposed by the present invention; Figure 3 This is a schematic diagram of the structure of a brain fixation plate in a novel fixation device for mouse brain imaging proposed by the present invention; Figure 4 Schematic diagram of the structure of the L-shaped plate in a novel fixing device for mouse brain imaging proposed by the present invention; Figure 5 Schematic diagram of the structure of the moving platform in a novel fixing device for mouse brain imaging proposed by the present invention; Figure 6 Schematic diagram of the structure of the heating pad in a novel fixing device for mouse brain imaging proposed by the present invention; Figure 7 Schematic diagram of the structure of the second fixing component in a novel fixing device for mouse brain imaging proposed by the present invention; Figure 8 Schematic diagram of the connection between the fixing table and the sliding pressing piece in a novel fixing device for mouse brain imaging proposed by the present invention; Fig. 9 Schematic diagram of the structure of the first adjusting component in a novel fixing device for mouse brain imaging proposed by the present invention; Fig.10 Schematic diagram of the connection between the height adjusting member, the second adjusting table and the third adjusting table in a novel fixing device for mouse brain imaging proposed by the present invention; Fig.11 Schematic diagram of the connection between the height adjusting member, the second adjusting table and the third adjusting table in a novel fixing device for mouse brain imaging proposed by the present invention; Fig.12 Schematic diagram of the internal structure of the moving platform in a novel fixing device for mouse brain imaging proposed by the present invention; In the drawings: 1. First fixing component; 10. L-shaped plate; 101. First connection groove; 102. First connection hole; 11. Support cylinder; 12. First connection plate; 13. Brain fixing piece; 130. Positioning hole; 131. Second connection groove; 2. Adjusting platform; 20. Installation platform; 21. First adjusting table; 22. Second adjusting table; 23. First adjusting knob; 24. Second adjusting knob; 25. Third adjusting knob; 26. Third adjusting table; 27. Height adjusting member; 271. Guide groove; 272. Rotating plate; 273. Fixing frame; 274. Connecting rod; 28. Lead screw; 29. Teeth; 3. Installation bottom plate; 4. First adjusting component; 40. L-shaped bracket; 41. Connecting piece; 42. Second connection plate; 5. Heating pad; 6. Second fixing component; 61. Third connection plate; 62. Support plate; 63. Sliding groove; 64. Connection table; 641. Fifth fixing hole; 642. Chute; 65. Sliding pressing piece; 66. Fixed rod; 7. Third fixing component; 70. First fixing groove; 71. Second fixing groove; 8. Adjusting hole. Detailed implementation manners

[0023] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0024] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0025] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0026] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0027] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0028] The embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0029] See also Figure 1 to Figure 10 The present invention proposes a novel fixing device for mouse brain imaging, which includes a first fixing component 1 for fixing the mouse head, the bottom of the first fixing component 1 is connected to an adjustment platform 2 for adjusting the tilt angle and position, the bottom of the adjustment platform 2 is connected to a mounting base plate 3, and a second fixing component 6 for fixing the mouse body is arranged on one side of the adjustment platform 2 on the mounting base plate 3, and one end of the second fixing component 6 is close to the first fixing component 1; in the present device, the first fixing component 1 is used to achieve efficient and stable fixation of the mouse head, prevent the mouse from moving during brain imaging, and improve the accuracy and reliability of imaging.

[0030] See also Figure 1~Figure 4 The first fixing assembly 1 includes a L-shaped plate 10, which is installed on the top of the adjustment platform 2. Four first connecting holes 102 are provided on the L-shaped plate 10, and each first connecting hole 102 is used to install a screw. The L-shaped plate 10 is stably fixed on the top of the adjustment platform 2 by the screw. Two first connecting grooves 101 are symmetrically provided on the upper end surface of the L-shaped plate 10 near the second fixing assembly 6, and each first connecting groove 101 passes through the bottom end surface of the L-shaped plate 10. A second fixing screw is installed inside, and the top end of the second fixing screw is fixedly connected to a supporting cylinder 11. The bottom end surface of the supporting cylinder 11 fits with the upper end surface of the L-shaped plate 10. The second fixing screw and the supporting cylinder 11 are connected by threads, and then the supporting cylinder 11 can be moved down the L-shaped plate 10 along the direction of the third adjusting groove by adjusting the second fixing screw. When the supporting cylinder 11 is damaged, it can be replaced by disassembling the second fixing screw, thereby increasing the practicality of the device.

[0031] See also Figure 1~Figure 4A first connecting plate 12 is connected to the top end of each supporting cylinder 11, and a second adjusting groove is provided on the first connecting plate 12 at the position corresponding to the end of the supporting cylinder 11, and a first fixing screw is installed in the second adjusting groove, and the bottom end of the first fixing screw is fixed to the top end surface of the supporting cylinder 11, and the first fixing screw is connected to the supporting cylinder 11 through a threaded connection, which is convenient for the disassembly between the first connecting plate 12 and the supporting cylinder 11, and then convenient for the assembly of the device, and a first adjusting groove is provided on the end of the first connecting plate 12 away from the second adjusting groove, and the first adjusting groove is connected to the second connecting groove 131 through a threaded rod, and a first fixing screw is provided near the end of the brain fixing plate 13 A second connecting groove 131 is provided, and a threaded rod is installed and fixed between the second connecting groove 131 and the corresponding first adjusting groove, so that a brain fixing plate 13 is connected between the two first connecting plates 12. The brain fixing plate 13 is fixed on the mouse head by a sufficient mixture of dental cement and glue. A positioning hole 130 is provided on the brain fixing plate 13. The positioning hole 130 is used to locate the window area for imaging the mouse brain. The positioning hole 130 is set to an inverted cone shape. The positioning hole 130 can avoid shadows in the imaging area due to the thickness of the fixing plate, thereby affecting experimental observation; in the process of fixing the mouse head, the positioning hole 130 on the brain fixing plate 13 is aligned with the mouse brain area that needs to be observed.

[0032] See also Figure 1 , Figure 2 , Figure 5 , Fig.10 , Fig.11 and Fig.12 The adjustment platform 2 includes a mounting platform 20, the mounting platform 20 is fixedly mounted on the mounting base plate 3, the top end of the mounting platform 20 is connected to a first adjustment platform 21, a first angle adjustment member is provided between the mounting platform 20 and the first adjustment platform 21, a first adjustment knob 23 is connected to the angle adjustment member, and the first adjustment knob 23 is rotatably mounted on the mounting platform 20; A third adjustment platform 26 is installed on the top of the first adjustment platform 21, a second angle adjustment member is installed between the first adjustment platform 21 and the third adjustment platform 26, a second adjustment knob 24 is connected to the second angle adjustment member, and the second adjustment knob 24 is rotatably installed on the first adjustment platform 21; The second angle adjusting member and the first angle adjusting member both include a lead screw 28. A lead screw 28 is installed in both the installation platform 20 and the first adjusting table 21. One end of the lead screw 28 located in the installation platform 20 is connected to the first adjusting knob 23, and one end of the lead screw 28 located in the first adjusting table 21 is connected to the second adjusting knob 24. Tooth teeth 29 are provided at the positions corresponding to the lead screw 28 on the bottom end face of the first adjusting table 21, and tooth teeth 29 are also provided at the positions corresponding to the lead screw 28 on the bottom of the third adjusting table 26. The lead screw 28 meshes with the corresponding tooth teeth 29. First arc-shaped grooves are provided on both sides of the lead screw 28 on the installation platform 20. First arc-shaped platforms are provided at the positions corresponding to the first arc-shaped grooves on the bottom end face of the first adjusting table 21. The first arc-shaped platforms are movably installed in the first arc-shaped grooves. By rotating the lead screw 28 and through the cooperation of the tooth teeth 29 and the first arc-shaped platforms and the first arc-shaped grooves, the first adjusting table 21 rotates on the installation platform 20; second arc-shaped grooves are provided on both sides of the lead screw 28 on the first adjusting table 21. Second arc-shaped platforms are provided at the positions corresponding to the second arc-shaped grooves on the bottom end face of the third adjusting table 26. The second arc-shaped platforms are movably installed in the second arc-shaped grooves. By rotating the lead screw 28 and through the cooperation of the tooth teeth 29 and the second arc-shaped platforms and the second arc-shaped grooves, the first adjusting table 21 rotates on the third adjusting table 26.

[0033] A second adjusting table 22 is installed on the top of the third adjusting table 26. A height adjusting member 27 is installed between the second adjusting table 22 and the third adjusting table 26. A third adjusting knob 25 is connected to the height adjusting member 27. The third adjusting knob 25 is rotatably installed on the first adjusting table 21. The top of the second adjusting table 22 is connected to an L-shaped plate 10. By rotating the third adjusting knob 25, the height adjusting member 27 works to adjust the distance between the second adjusting table 22 and the third adjusting table 26, thereby raising the second adjusting table 22 to a certain height; the height adjusting member 27 includes a fixed frame 273. An installation cavity is provided on the upper end face of the third adjusting table 26. The fixed frame 273 is installed on the inner wall of the installation cavity. One end of a connecting rod 274 is vertically movably inserted through the fixed frame 273. The other end of the connecting rod 274 is fixed to the bottom end face of the second adjusting table 22. The connecting rod 274 is L-shaped. A connecting column is provided at one end of the connecting rod 274 close to the fixed frame 273. The connecting column is movably installed in a guide groove 271. The guide groove 271 is provided on a rotating plate 272. The rotating plate 272 is fixedly installed on the inner end head of the third adjusting knob 25. By rotating the third adjusting knob 25, the rotating plate 272 rotates, causing the connecting column to move in the guide groove 271, thereby causing the connecting rod 274 to vertically move in the installation cavity and raising the second adjusting table 22. An expansion rod is provided on the top end face of the third adjusting table 26 at the side of the installation cavity. One end of the expansion rod is fixed to the top end face of the third adjusting table 26, and the other end of the expansion rod is fixed to the bottom end of the second adjusting table 22.

[0034] See also Figure 1 , Figure 6 , Figure 7 , Figure 8 and Fig. 9 The second fixing component 6 includes a support plate 62 for placing the mouse body, and a plurality of fixing components are installed on the upper end surface of the support plate 62. The plurality of fixing components are arranged side by side, and each fixing component includes two connecting platforms 64. The upper end surface of the support plate 62 is symmetrically provided with a sliding groove 63, and the connecting platform 64 is installed in the sliding groove 63. The connecting platform 64 is provided with a fifth fixing hole 641, and a fixing rod 66 is installed in the fifth fixing hole 641. One end of the fixing rod 66 is close to the installation base plate 3. In addition, the mouse body is strengthened and fixed by wrapping a restraint belt around the sliding groove 63. After the connecting platform 64 is adjusted in the sliding groove 63 according to the body position of the mouse, the connecting platform 64 is fixed by the fixing rod 66; When fixing the mouse body, the mouse body is located between the two connecting platforms 64 in each group, wherein a connecting platform 64 in each group of fixing parts is provided with a slide groove 642, and one end of a sliding pressure plate 65 is connected to the other connecting platform 64, and the other end of the sliding pressure plate 65 passes through the slide groove 642 and is fixed by a fixing rod 66, and a plurality of threaded holes are evenly spaced on the sliding pressure plate 65 near the slide groove 642, one of the threaded holes is connected to one end of the fixing rod 66, so that the sliding pressure plate 65 fixes the mouse body; by adjusting the sliding pressure plate 65, the device can be suitable for fixing mice of different sizes, thereby achieving comfortable and firm fixation of the mouse body, while ensuring the comfort and safety of the mouse during the imaging process.

[0035] See also Figure 1 and Figure 7 A third fixing component 7 is provided on the support plate 62 near the end of the brain fixing plate 13. The third fixing component 7 includes a first fixing groove 70 and a second fixing groove 71. The first fixing groove 70 is used to insert the lower jaw of the mouse after the mouse's mouth is opened, and the second fixing groove 71 is used to clamp the mouse's incisors. Together with the first fixing component 1, the mouse's head can be fixed more stably, providing accuracy and reliability of mouse brain imaging and preventing the mouse from struggling to get rid of the brain fixing plate 13.

[0036] See also Figure 1 , Figure 7 , Figure 8 and Fig. 9A first adjusting component 4 is connected to the side end surface of the support plate 62 relative to the connecting platform 64, and the first adjusting component 4 is installed on the mounting base plate 3. The first adjusting component 4 includes an L-shaped bracket 40, and a plurality of adjusting holes 8 are provided on the mounting base plate 3. The plurality of adjusting holes 8 are symmetrically arranged about the central axis of the mounting base plate 3. A first fixing hole is provided on the L-shaped bracket 40, and a threaded rod is installed in the first fixing hole on the L-shaped bracket 40, and the threaded rod is connected with the corresponding adjusting hole 8 to fix the L-shaped bracket 40 to the mounting base plate 3. The first fixing hole on the L-shaped bracket 40 is respectively connected with different adjusting holes 8 through threaded rods, so that the position of the support plate 62 can be adjusted. After the first adjusting component 4 fixes the mouse head, the position of the support plate 62 is adjusted through the adjusting hole 8, so that the mouse body is stably fixed on the support plate 62. A second A fixing hole, a connecting piece 41 is installed in the second fixing hole, a second connecting plate 42 is connected to the connecting piece 41, a third fixing hole is provided on the second connecting plate 42, the connecting piece 41 connects the second connecting plate 42 to the side end face of the L-shaped bracket 40 through the third fixing hole and the second fixing hole, another connecting piece 41 is also installed on the third fixing hole, a third connecting plate 61 is connected to the connecting piece 41, a fourth fixing hole is provided on the third connecting plate 61, the connecting piece 41 connects the third connecting plate 61 to the side end face of the second connecting plate 42 through the third fixing hole and the fourth fixing hole, the upper end of the third connecting plate 61 is fixed to the side end face of the support plate 62, the first adjusting component 4 enables the support plate 62 to be adjusted in angle on the mounting base plate 3, so that the mouse can be comfortably fixed on the support plate 62, the resistance of the mouse is reduced, and the stability of the molding is improved; See also Figure 1 , Figure 6 and Figure 7 A heating pad 5 is provided on the support plate 62 to ensure that the mice maintain normal vital signs during the experiment, provide a comfortable imaging environment for the mice, and reduce imaging interference caused by temperature discomfort.

[0037] The working method of the device is as follows: according to the experimental requirements, the mice are anesthetized to different degrees or in different ways, the anesthetized mice are placed on the heating pad 5 with the power turned on through the connecting platform 64, after the mouse's mouth is opened, the first fixing groove 70 is used to pass the mouse's lower jaw, and the second fixing groove 71 is used to clamp the mouse's incisors to achieve preliminary fixation of the mouse's head, the sliding pressing plate 65 on the connecting platform 64 is adjusted to be placed in a suitable position, the sliding pressing plate 65 is fixed to the mouse body through the fixing rod 66, and the positioning hole 130 of the brain fixing plate 13 is fixed to the mouse brain observation imaging area through dental cement, and according to the experimental needs, the X, Y, and Z direction positions of the adjustment platform 2 are adjusted by turning the first adjustment knob 23, the second adjustment knob 24, and the third adjustment knob 25, and the second fixing component 6 is adjusted to adjust the relative position of the L-shaped plate 10 and the supporting cylinder 11, so that the brain fixing plate 13 can be reasonably fixed on the first connecting plate 12 through the positioning hole 130, thereby completing the fixation of the mouse.

[0038] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0039] In addition, it should be understood that although this specification is described in accordance with the implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation modes that can be understood by those skilled in the art. The above content is only to illustrate the technical idea of ​​the present invention, and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution according to the technical idea proposed by the present invention shall fall within the protection scope of the claims of the present invention.

Claims

1. A novel fixation device for mouse brain imaging, characterized in that: The invention comprises a first fixing component (1) for fixing the head of a mouse, the bottom of the first fixing component (1) is connected to an adjustment platform (2) for adjusting the tilt angle and position, the bottom of the adjustment platform (2) is connected to a mounting base plate (3), a second fixing component (6) for fixing the body of the mouse is arranged on one side of the adjustment platform (2) on the mounting base plate (3), and one end of the second fixing component (6) is close to the first fixing component (1); The first fixing component (1) comprises a L-shaped plate (10), wherein the L-shaped plate (10) is mounted on the top of the adjusting platform (2), wherein two supporting cylinders (11) are symmetrically arranged on the L-shaped plate (10) near the second fixing component (6), wherein the top of each supporting cylinder (11) is movably connected to a first connecting plate (12), and a brain fixing plate (13) is connected between the two first connecting plates (12), wherein the brain fixing plate (13) is fixed to the head of the mouse, and wherein a positioning hole (130) is arranged on the brain fixing plate (13), wherein the positioning hole (130) is used to position a window area for imaging the mouse brain.

2. A novel fixation device for mouse brain imaging according to claim 1, characterized in that: A second connecting groove (131) is provided on the brain fixing plate (13) at a position close to the end thereof, and a first adjusting groove is provided on the first connecting plate (12) at a position corresponding to the end of the brain fixing plate (13), and the first adjusting groove and the second connecting groove (131) are connected via a connecting screw.

3. A novel fixation device for mouse brain imaging according to claim 2, characterized in that: A second adjustment slot is provided on the end of the first connecting plate (12) away from the first adjustment slot, a first fixing screw is installed in the second adjustment slot, and the bottom end of the first fixing screw is fixed to the top of the supporting column (11).

4. A novel fixation device for mouse brain imaging according to claim 1, characterized in that: A third adjustment groove is provided on the end of the L-shaped plate (10) close to the second fixing component (6), a second fixing screw is connected in the third adjustment groove, and the top end of the second fixing screw is fixed to the top of the supporting column (11).

5. A novel fixation device for mouse brain imaging according to claim 1, characterized in that: The adjustment platform (2) comprises a mounting platform (20), the mounting platform (20) being fixedly mounted on the mounting base plate (3), a first adjustment platform (21) being mounted on the top of the mounting platform (20), a first angle adjustment member being arranged between the mounting platform (20) and the first adjustment platform (21), a first adjustment knob (23) being connected to the first angle adjustment member, and the first adjustment knob (23) being rotatably mounted on the mounting platform (20); A third adjustment platform (26) is installed on the top of the first adjustment platform (21); a second angle adjustment member is provided between the third adjustment platform (26) and the first adjustment platform (21); a second adjustment knob (24) is connected to the second angle adjustment member; and the second adjustment knob (24) is rotatably mounted on the first adjustment platform (21); A second adjustment platform (22) is installed on the top of the third adjustment platform (26); a height adjustment member (27) is provided between the second adjustment platform (22) and the third adjustment platform (26); a third adjustment knob (25) is connected to the height adjustment member (27); and the third adjustment knob (25) is installed on the third adjustment platform (26).

6. A novel fixation device for mouse brain imaging according to claim 1, characterized in that: The second fixing component (6) comprises a support plate (61) for placing the mouse body, and a plurality of groups of fixing components are installed on the upper end of the support plate (61), each group of fixing components comprises two connecting platforms (64), and the mouse body is located between the two connecting platforms (64) in each group, wherein one connecting platform (64) in each group of fixing components is provided with a slide groove (642), and another connecting platform (64) is connected to one end of a sliding pressing plate (65), and the other end of the sliding pressing plate (65) passes through the slide groove (642) and is fixed by the fixing rod (66), so that the sliding pressing plate (65) fixes the mouse body; A first adjustment component (4) is connected to the side end surface of the support plate (61) opposite to the connection platform (64), and the adjustment component (4) is mounted on the mounting base plate (3).

7. A novel fixation device for mouse brain imaging according to claim 6, characterized in that: The support plate (61) is symmetrically provided with sliding grooves (63), the connecting platform (64) is installed in the sliding grooves (63), the connecting platform (64) is provided with a fifth fixing hole (641), a fixing rod is installed in the fifth fixing hole (641), and one end of the fixing rod is closely attached to the mounting base plate (3); A third fixing member (7) is provided on the support plate (61) at a position close to the end of the brain fixing plate (13), and the third fixing member (7) comprises a first fixing groove (70) and a second fixing groove (71), wherein the first fixing groove (70) is used to insert the lower jaw of the mouse after the mouse's mouth is opened, and the second fixing groove (71) is used to clamp the mouse's incisors.

8. A novel fixation device for mouse brain imaging according to claim 6, characterized in that: The first adjustment assembly (4) comprises an L-shaped bracket (40), the L-shaped bracket (40) being mounted on the mounting base plate (3), a connecting piece (41) being connected to one side of the L-shaped bracket (40), a second connecting plate (41) being connected to the connecting piece (41), a third connecting plate (61) being connected to the second connecting plate (41), and the third connecting plate (61) being fixed to a side end surface of the support plate (61).

9. A novel fixation device for mouse brain imaging according to claim 8, characterized in that: A plurality of adjustment holes (8) are provided on the installation base plate (3), and the L-shaped bracket (40) is connected to the corresponding adjustment holes (8) via threaded rods.

10. A novel fixation device for mouse brain imaging according to claim 6, characterized in that: A heating pad (5) is provided on the support plate (62).

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