52 results about "Mice brain" patented technology

The invention discloses application of eugenin in preparation of a medicine for treating cerebral arterial thrombosis, and belongs to the technical field of medicines. An in-vivo middle cerebral artery occlusion model is adopted, after reperfusion is conducted for 24 h, the cerebral infarction volume and movement coordination function defect of a mouse are detected to evaluate the treatment effect of eugenin, and activation of microglial cells and survival of neuronal cells are observed through immunofluorescence to clarify the action way of eugenin. Pharmacological activity shows that eugenin can obviously reduce the cerebral infarction volume of a model mouse, improve pathological damage of cells and promote recovery of a motion coordination function, and the action way of eugenin is to promote survival of neuronal cells and inhibit activation of microglial cells. Research results indicate that eugenin has the effect of resisting cerebral arterial thrombosis and can be used as a candidate medicine for developing the cerebral arterial thrombosis resisting medicine.

The invention discloses a method for detecting mouse brain nucleus activation based on manganese-enhanced magnetic resonance imaging, and belongs to the field of image processing, and the method comprises the steps: converting an acquired mouse head manganese-enhanced magnetic resonance image into an NIFTI format, and enabling the direction and voxel size of the image to be consistent with a standard mouse brain map template; performing offset field correction on the image; loading a PLKA-nnUNet model, carrying out image segmentation on the image, outputting a binary brain mask, and extracting an individual mouse brain image from the image after bias field correction; carrying out image registration and intensity normalization; for each registered individual mouse brain image, calculating relaxation rate mean values of four hippocampal subregions of the mouse brain R1 image, wherein the relaxation rate mean values are used for quantitatively comparing mouse brain activation conditions; and carrying out voxel-level statistical test on the registered and normalized individual mouse brain images to identify brain regions with intensity differences among different experimental conditions. According to the method, the dependence on professional operation is reduced.

The application discloses a photosensitizer targeting beta amyloid, and a preparation method and application thereof. The photosensitizer is a photosensitizer with A-D-A configuration, and a structural formula thereof is shown as formula I. The photosensitizer synthesized in the application has a longer emission wavelength, and after being combined with beta amyloid, the fluorescence signal is obviously enhanced, and can effectively generate singlet oxygen, that is, the photosensitizer can target mark A beta protein, and is used for fluorescence imaging of the A beta protein; and after the photosensitizer synthesized in the application is combined with beta amyloid in the brain of an Alzheimer's disease (AD) model mouse, the cognitive ability of the AD mouse can be effectively improved, A beta-mediated neurotoxicity can be reduced, and clearance of A beta plaques can be promoted, that is, the photosensitizer can be used for early diagnosis and treatment of Alzheimer's disease.

The invention relates to the technical field of biomedical engineering, in particular to an invasive mouse electroencephalogram data acquisition and analysis system and method. The system comprises a motion structure assembly used for providing a motion carrier for a mouse and collecting motion parameters; the control assembly is used for adjusting motor output according to the target parameter, generating a synchronous time label instruction, recording the actual motion parameter in real time and storing the actual motion parameter in association with the time label; and the data acquisition and analysis component is used for acquiring original electroencephalogram data, synchronously storing a time label, aligning an electroencephalogram sampling time sequence with a motion control time label sequence, extracting electroencephalogram pulse position information, calculating a neuron discharge frequency and establishing a correlation between the discharge frequency and a motion parameter. According to the technical scheme, accurate and synchronous acquisition, automatic alignment of time stamps and multi-dimensional correlation analysis of mouse motion states and electroencephalogram signals can be realized.

The invention belongs to the technical field of biology, and particularly relates to a construction method of a respiratory syncytial virus infected mouse model. Disodium clodronate liposome with the concentration of 4ng / mL is injected into the lateral ventricle of the mouse through stereotactic positioning of the brain. 48h after injection, mouse brain tissues are taken to detect the activation state of microglial cells through immunofluorescence and WB, after it is confirmed that the microglial cells are inhibited, RSV (virus titer: 2.8 * 10 < 6 > PFU) nasal drop infection experiments are carried out on the mouse, and after the 5th day after virus infection, the mouse is infected by the RSV (virus titer: 2.8 * 10 < 6 > PFU) nasal drop infection experiments. Likewise, mouse brain tissue is taken for virus load detection, and neuropathological changes of the mouse brain tissue are observed through HE staining, immunofluorescence and TUNEL staining, so that the method can be used for constructing a research model of RSV-related nervous system diseases.

This invention discloses a method for preparing NK cells, the NK cells obtained thereby, and their applications. The method includes: sorting NK cells from peripheral blood of an allogeneic donor; culturing them in a serum-free medium containing CD3 monoclonal antibody, CD16 antibody, and IL-2, without the need for a feeder layer; preferably, IL-21 is added for amplification on days 3-5 of culture, and the cells are cultured for 20-40 days. This method yields non-genetically modified NK cells, among which CD56... bright The NK cells comprise >90% of the cell line and highly express a variety of activating and functional molecules. These NK cells effectively clear Aβ oligomers and senescent cells, and alleviate neuroinflammation. In treatment, intracranial injection achieves equivalent or better efficacy with only about 1 / 10th the dose administered intravenously, significantly clearing Aβ deposits in the brains of AD model mice and improving pathology.

This invention relates to animal models and their applications, specifically to a method for constructing a spontaneous mouse cerebral edema model and its application. This invention utilizes PEN2... fl / + Mice and PEN2 fl / + Mice were mated to obtain PEN2. fl / fl Homozygous mice; then the obtained PEN2 fl / fl Mice and hGFAP Cre / + Mice were mated to obtain PEN2. fl / + hGFAP Cre / + Mice; the final PEN2 obtained fl / + hGFAP Cre / + Mice and PEN2 fl / fl Mice were mated to obtain PEN2. fl / fl hGFAP Cre / + The mouse model is a spontaneous mouse cerebral edema model. In this model, PEN2 protein levels are significantly downregulated, and the mouse exhibits a severe cerebral edema phenotype. This model can be used to establish a cerebral edema drug screening platform. It can also be used for research on cerebral edema-related diseases, such as the pathogenesis of cerebral edema caused by infection, glioma, and traumatic brain injury, as well as for the development of technologies and products for the diagnosis and treatment of related diseases.

The application discloses a kind of mouse brain ischemic lesion automatic quantification method and system based on deep learning, belong to medical image processing and artificial intelligence technical field.The method includes: standardization pretreatment is carried out to T1WI, T2WI, DTI and ASL and other multi-modal MRI data, and enhanced feature tensor is constructed;Lesion intelligent preliminary segmentation is carried out using BA-U-Net network guided by physical prior, and biophysical consistency loss is introduced;Boundary refining is carried out to segmentation result using graph convolution network;Based on LDDMM registration and neural ordinary differential equation model, dynamic evolution modeling is carried out to multi-time point lesion, and severity score is calculated;Finally, comprehensive pathophysiological feature vector is constructed, XGBoost model is used for prediction, and interactive report containing 3D visual model is generated by SHAP explanation.The system includes corresponding functional module.The application realizes high-precision, reproducible automatic quantification to fuzzy lesion, improves the robustness of pathological pattern recognition, and provides dynamic biomarker for drug evaluation.

The invention discloses a fluorescent / electrochemical dual-mode probe for detecting hydrogen peroxide (H2O2) as well as a preparation method and application of the fluorescent / electrochemical dual-mode probe, and belongs to the technical field of organic probe molecular analysis and detection. The probe takes resorufin (Re) as a fluorescence and electrochemical double-signal report unit and pentafluorobenzenesulfonyl (PS) as an H2O2 response unit, and is named as Re-PS. The preparation process comprises the following steps: under the conditions of nitrogen protection and ice bath, resorufin and pentafluorobenzene sulfonyl chloride react in an anhydrous dichloromethane solvent under the catalysis of triethylamine, and a target product is obtained through rotary evaporation concentration and silica gel column chromatography purification. The probe has both open-type fluorescence detection and ratio-type electrochemical detection functions, and is used for quantitative analysis of H2O2 in Alzheimer's disease (AD) model mouse brain microdialysate. Compared with the existing detection technology, the probe provided by the invention has high stability (37 DEG C, pH 7.4), and the hydrolysis rate lt in 15 min; the method has the advantages of high sensitivity (5%), high selectivity (no obvious response to other active oxygen and biomolecules) and accurate detection (dual-mode signal cross validation and recovery rate of 96.0%-103.0%), provides a reliable tool for accurate detection of H2O2 in biological samples, and has important application value in neurodegenerative disease mechanism research and related diagnosis and treatment evaluation.

This invention proposes a novel fixation device for mouse brain imaging, comprising a first fixation component, an adjustment platform connected to the bottom of the first fixation component, a mounting base plate connected to the bottom of the adjustment platform, and a second fixation component mounted on the mounting base plate, with one end of the second fixation component close to the first fixation component. The first fixation component includes an L-shaped plate mounted on the top of the adjustment platform. Two supporting cylinders are symmetrically arranged on the L-shaped plate near the second fixation component. The top of each supporting cylinder is movably connected to a first connecting plate. A brain fixation plate is connected between the two first connecting plates and fixed to the mouse's head. The brain fixation plate has positioning holes for locating the viewing window area for mouse brain imaging. This device reduces stress response in mice during experiments, improves experimental reliability and repeatability, ensures absolute stability of mice in an awake state, and effectively improves the accuracy of experimental data.

The invention discloses an application of circWHSC1 as a biomarker in preparation of a product for diagnosing or treating ineffective recanalization after ischemic stroke. According to the present invention, the circRNA sequencing results show that the expression of the circWHSC1 (including hsacirc0001388 and nucirc0001336) in the ineffective recanalization patient body is significantly reduced, and the circWHSC1 is verified in the mouse brain tissue, the plasma and the thrombus model of the tMCAO model; functional studies show that the circWHSC1 can significantly reduce the cerebral infarction volume of the mouse. The circWHSC1 can be used for early diagnosis, risk assessment, severity judgment, curative effect monitoring and prognosis judgment of invalid recanalization, and can be used as a drug target for screening candidate drugs for preventing or treating invalid recanalization. The invention provides a molecular diagnosis marker and a therapeutic target for invalid recanalization, and has important clinical value.

A co-culture model of brain glioma organoids and mouse brain slices, characterized in that the co-culture model is prepared by the following method: (1) taking mouse brain slices, culturing to obtain cultured mouse brain slices; (2) injecting brain glioma organoids into the cultured mouse brain slices obtained in step (1) to obtain brain slices injected with brain glioma organoids; (3) culturing the brain slices injected with brain glioma organoids obtained in step (2) to obtain the co-culture model. The co-culture model of the application can be used to evaluate the invasion phenotype of glioma organoids in vitro and to test and screen brain glioma drugs.

The invention relates to an in vitro method of generating a murine cerebral organoid cell culture model, comprising cultivating murine neural stem cells obtained from an adult mouse until neurospheres have formed, and subsequently differentiating said neurospheres, thereby generating a murine cerebral organoid cell culture model. The invention further relates to an in vitro murine cerebral organoid cell culture model comprising mature astrocytes, oligodendrocytes and / or neurons produced according to the method of the invention and its use for drug or toxicity screenings or for studying neurological diseases. In other aspects the invention further relates to kits, cell culture media and supplement compositions comprising components to be used in the context of the present invention.

The application provides an animal brain metabolite identification method based on mass spectrometry imaging technology and application, and establishes a mouse brain tissue slice endogenous metabolite DESI-MSI analysis method based on mass spectrometry imaging technology, which comprises the following steps: after preparing metabolite control sample imaging samples and brain tissue slice imaging samples, multiple animal brain tissues are attached to the same glass slide, mass spectrometry imaging condition optimization is carried out by using the control sample imaging samples, and mass spectrometry imaging analysis is carried out by using the brain tissue slice imaging samples; the image of the biological sample is divided into multiple structure regions in combination with tissue morphological characteristics; for the structure regions, multivariate statistical analysis is carried out to obtain different metabolites in different structure regions; metabolite identification is carried out based on parent ions and characteristic daughter ion profiles; and the method is used for disease mechanism research and drug intervention effect evaluation on diseases.

The application discloses application of an S1PR1 selective agonist SAR247799 in preparation of a drug for treating neuromyelitis optica spectrum disorders. The SAR247799 can up-regulate S1PR1 expression of astrocytes, activate an S1PR1 signal path of the astrocytes, inhibit AQP4-IgG and complement-mediated damage of the astrocytes, reduce loss of AQP4, GFAP and ALDH1L1 in a lesion area of brain tissue of a neuromyelitis optica spectrum disorder model mouse, and relieve pathological damage of the neuromyelitis optica spectrum disorder model. In-vivo and in-vitro experiments prove that the SAR247799 can effectively inhibit AQP4-IgG and complement-dependent cytotoxicity effects and promote survival of the astrocytes. The application provides a novel, efficient and specific targeted drug for clinical treatment of the neuromyelitis optica spectrum disorder.

This invention belongs to the field of organic probe molecular analysis and detection technology, and discloses a fluorescence / electrochemical dual-mode probe for detecting copper ions (Cu2+), its preparation method, and its application. The probe's molecular structure is as follows: Under argon protection and an ice-salt bath, halogenated hydroxyl chloride and pyridinecarboxyl chloride hydrochloride undergo an esterification reaction in anhydrous dichloromethane solvent catalyzed by triethylamine. The target product is obtained after extraction, drying, vacuum concentration, and column chromatography purification. This probe combines "on-screen" fluorescence detection with ratiometric electrochemical detection, enabling highly sensitive and selective quantitative analysis of free Cu2+ in complex biological samples such as dialysis fluid from Alzheimer's disease (AD) model mice. Compared to existing detection technologies, this probe has advantages such as high selectivity, low detection limit, good stability, and accurate dual-signal cross-validation results, providing a reliable tool for the accurate detection of Cu2+ in biological samples.

The invention relates to a near-infrared band ratio type rare earth fluorescent nanoprobe as well as a preparation method and application thereof. The ratio-type rare earth fluorescent probe is a nanocrystal with a core-shell structure, an inner core layer of the ratio-type rare earth fluorescent probe is a light-emitting layer which absorbs laser radiation and emits a near-infrared fluorescent wave band, a middle shell layer of the ratio-type rare earth fluorescent probe is a light-emitting shell layer which absorbs laser radiation and emits another near-infrared fluorescent wave band, and an outer shell layer of the ratio-type rare earth fluorescent probe is an inert shell layer. Wherein the inner core layer takes Tm < 3 + > as a light-emitting center, and the light-emitting shell layer takes Er < 3 + > as a light-emitting center. Through difference response of two fluorescence signal wave bands to water absorption, an index relation between ratio-type fluorescence signal intensity and tissue penetration depth is established, and noninvasive depth positioning and imaging of a mouse living body are realized. The fluorescent nanoprobe can be used for depth positioning of blood vessels of the brain, the abdomen and the legs of a mouse and continuous monitoring of a dynamic migration process from the blood vessels to bones, and has a wide application prospect in the aspect of in-vivo ratio depth imaging.

The invention discloses a mouse cranial nerve cell axon segmentation method based on a frozen section technology. The method comprises the following steps: step (1) cell culture; (2) quickly freezing the sample and the cutter; (3) sample block trimming; (4) slicing the sample wafer; and (5) taking and transferring the wafer. According to the method, a Leica ultrathin slicing machine is used as processing equipment, a micro-nano slicing technology is combined with a freezing module, the temperature in a cavity is stabilized at-130 DEG C through liquid nitrogen and a temperature control system, a PDMS micro-fluidic chip containing mouse cerebral nerve cells is put into the cavity to be frozen, PDMS is converted into a glassy state easy to cut, and then the PDMS micro-fluidic chip containing mouse cerebral nerve cells is obtained. And cutting the part containing the cerebral nerve cell axon section in the sample into a film of 500 nm to 10 [mu] m by using a glass linear blade cutter as required, so as to realize normal form transformation from separation analysis to in-situ analysis.

The invention discloses a method for diagnosing and treating AD (Alzheimer's disease) by recognizing olfactory disorder through PET (Polyethylene Terephthalate). The method comprises the following steps: (1) observing olfactory and cognitive behavioral changes of AD mice, and evaluating olfactory and cognitive function conditions of the 3 * Tg mice by carrying out behavioral experiments on the 12-month-old 3 * Tg mice; and (2) quantifying A beta in the intracranial olfactory related area of the AD mouse through PET imaging, performing A beta-PET imaging on a 12-month-old 3xTg mouse, reconstructing a PET image through a PET / CT scanner, performing SUV quantification on the intracranial olfactory related area, and observing the deposition condition of the A beta in the olfactory related area. And (3) observing A beta and tau phosphorylation expression and distribution conditions of AD mice in pathology, observing A beta expression and distribution conditions in brains of 12-month-old 3xTg mice through immunofluorescence, dividing olfaction-related regions by referring to an Allen mouse brain map, observing expression conditions of A beta in the olfaction-related regions, and calculating average fluorescence intensity of each region for semi-quantitative analysis.

The invention discloses a method for detecting 19 amino acids in brain tissue and bone marrow of a mouse. The method comprises the following steps: step 1, preparing a standard substance; step 2, sampling brain tissues and bone marrow of the mouse; step 3, removing proteins in brain tissues and bone marrow samples; step 4, preparing a derivatization reagent; step 5, separating and determining 19 amino acids in the brain tissue and the bone marrow sample by HPLC-FLD; and 6, calculating a result. The method for detecting 19 amino acids in brain tissues and bone marrow of mice has the advantages of simple operation steps, high accuracy and good sensitivity, and can realize accurate detection of 19 amino acids in brain tissues and bone marrow samples.

The invention discloses a method for establishing an inflammation model of a Gga3 gene knockout mouse and application, and relates to the technical field of biological medicine, and the key point of the technical scheme is as follows: it is found that Gga3 gene knockout significantly reduces inflammation in the brain of the mouse caused by LPS, and it is prompted that GGA3 plays an important role in regulating and controlling neuroinflammation. The regulation mechanism of GGA3 in microglial cell mediated neuroinflammation can be better illuminated, and the GGA3 has a very important effect on finding drug targets of related neurodegenerative diseases.

The invention belongs to the field of biotechnology medicine, and particularly relates to a method for inducing astrocytes to be reprogrammed into neurons, which comprises the following steps: cutting mouse brain tissues into pieces, adding protease for digestion, filtering, inoculating, and culturing in a constant-temperature incubator. And after the cells reach the standard, carrying out passage, taking third-generation astrocytes, digesting and resuspending, carrying out virus infection, and recording infection conditions and MOI of a well-growing group. The method comprises the following steps of: performing cell starvation treatment, firstly adding a CMV-dcas9-VPR-mCherry virus, then adding a U6-gRNA-EGFP virus for overexpression of Ngn2, Prdx2 and Sod1 genes, and selecting a proper neuron culture solution for culture according to induction time; the overexpressed Ngn2 can convert astrocytes into mature neurons with normal electrophysiological functions. The additional overexpression of Prdx2 and Sod1 can improve the reprogramming efficiency, accelerate the speed and promote the maturation of neurons. The mechanism is that ferroptosis can be reduced along with ferroptosis Prdx2 and Sod1 in the process, so that the reprogramming efficiency and the long-term survival rate are improved.

This invention discloses a method for preparing Huangda tea polysaccharide L2-1 and its use in preventing and improving Alzheimer's disease. Huangda tea polysaccharide L2-1 has a total sugar content of 91.89%, a protein content of 1.19%, and a uronic acid content of 13.18%. The monosaccharide composition and its molar ratio are arabinose:rhamnose:mannose:glucose:galacturonic acid = 0.169:0.256:0.464:1:1.396. The Huangda tea polysaccharide L2-1 prepared by this invention can significantly improve the survival rate of L-Glu-induced PC12 cells and restore cell morphology, enhance the learning and memory abilities of APP / PS1 transgenic mice, inhibit the excessive activation of astrocytes in mouse brain tissue, and reduce neuroinflammation in the mouse brain. Huangda tea polysaccharide L2-1 has the function of improving / treating Alzheimer's disease.

The invention discloses a probe molecule capable of selectively binding / marking amyloid protein, a preparation method and application of the probe molecule in brain slices of mice suffering from neurodegenerative diseases, and the probe molecule has a structure as shown in a formula I. In the formula I, the value range of n is 0 or 1; r is selected from at least one of p-aminobenzene, p-dimethylaminobenzene, julolidine and 2-dimethylaminonaphthalene. The invention designs a series of photosensitive labeling probes with amyloid variant protein binding capacity. The probe can generate a large amount of active oxygen under the condition of illumination. A marking substrate with nucleophilicity is added into a reaction system, and the probe can realize selective marking of amyloid protein. The probe can realize component analysis of amyloidosis protein in brain slices of mice suffering from Alzheimer's disease and component comparison of amyloidosis protein of mice with different neurodegenerative disease models.

The invention discloses bacoportulacin 2 targeting an AQP1 target spot after mouse cerebral apoplexy and application of bacoportulacin 2 in cerebral apoplexy intervention, and relates to the technical field of biological medicines, and the action mechanism of the medicine is targeted inhibition of expression and / or functions of aquaporin 1; according to the invention, the application of bacoportulacin 2 is associated with a highly expressed AQP1 target in astrocytes after specific inhibition of cerebral apoplexy, and it is further revealed that the bacoportulacin 2 can play a role by regulating the pathogenicity interaction between AQP1 and downstream protein 4EBP2. The dual-action mechanism of'upstream target inhibition-downstream interaction interference 'provides a new thought and a clear intervention node for developing a collaborative treatment drug aiming at a complex pathological network of ischemic brain injury, and surpasses a traditional strategy of single target inhibition.

The invention relates to the technical field of plastic brain toxicity detection, and discloses a plastic brain toxicity detection method, which comprises the following steps: giving a preset number of food particles and water to mice in a target environment, and randomly dividing the mice into groups; after the mice are weighed, MNP with a preset concentration is administered to the experimental group regularly every day, normal saline is administered to the control group, 3D behavioral recording is conducted on the mice after several days of continuous administration, and behavioral analysis is conducted according to the recording result; and after behavioral analysis, performing injection anesthesia on the mice in each group, sampling brain tissues of the mice in each group, and performing brain cell analysis based on the brain tissues of the mice so as to detect the plastic brain toxicity. The toxicity of micro-plastic or nano-plastic can be efficiently and accurately detected through systematic experiment steps.

The invention discloses a method for establishing a model for inflammation of mouse brain microvascular endothelial cells infected by haemophilus parasuis, which is used for simulating the process of porcine meningitis caused by haemophilus parasuis infection by using the mouse brain microvascular endothelial cells infected by haemophilus parasuis and researching the pathogenesis of the porcine meningitis caused by haemophilus parasuis. The optimal MOI of mouse brain microvascular endothelial cells infected by haemophilus parasuis is 1, the optimal infection time is 6 h, under the condition, the inflammatory factor level of the cells is remarkably increased, and the success rate of the cell inflammation model construction method is higher.

The invention discloses siRNA of targeted neutrophil HMGB2, a delivery system and a construction method and application of the delivery system, and belongs to the technical field of biological medicine. The siRNA of the targeted neutrophil HMGB2 comprises a positive-sense strand and an antisense strand, and the sequences of the positive-sense strand and the antisense strand are respectively SEQ ID NO: 1 and SEQ ID NO: 2. The siRNA expressed by HMGB2 target genes is prepared, lipid nanoparticles with a neutrophil targeting function are further provided to wrap the siRNA to serve as a delivery system, HMGB2 gene expression in neutrophil in the brain of a mouse after stroke is remarkably inhibited, brain injury of the mouse after stroke is relieved, and a new potential medicine is provided for treatment of stroke.