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A stirred bioreactor for sonic cultivation of microorganisms

A technology of bioreactors and microorganisms, which is applied in the direction of specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, method for stimulating microbial growth by stress, etc. Stirred bioreactors and other issues to achieve the effects of accelerated metabolic activities, increased yield, and good cultivation effects

Inactive Publication Date: 2016-05-18
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have shown that sound waves can promote the growth of microorganisms, but there is no special fermentation culture bioreactor for sonic culture, especially no stirred bioreactor that can obtain ideal culture effects

Method used

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  • A stirred bioreactor for sonic cultivation of microorganisms
  • A stirred bioreactor for sonic cultivation of microorganisms
  • A stirred bioreactor for sonic cultivation of microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Application of Stirred Bioreactor for Microorganism Acoustic Cultivation in Increasing the Production of Microbial Metabolites and Comparison of Production Results

[0028] Microbial material: liquid strain of Streptomyces natalis

[0029] Medium: 1. Plate and slant medium g / L: glucose 10, wort juice 3, yeast extract 3, peptone 5, agar 20, pH7.0~pH7.3; 2. Seed medium g / L: glucose 20 , soybean peptone 10, yeast extract 6, sodium chloride 10, pH7.3; 3. Fermentation medium g / L: soybean cake powder 40, glucose 20, soluble starch 40, yeast extract 7, pH7.0.

[0030]Operation steps: Divide the microorganism Streptomyces natalis liquid bacteria into two groups, the first group adopts the traditional culture method to cultivate; the second group adopts the stirred bioreactor for the sonic culture of microorganisms of the present invention to cultivate, the specific operation The method is as follows: first pass steam through the air inlet 14 to sterilize the empty ta...

Embodiment 2

[0034] Example 2, the application of the stirred bioreactor for the sonic culture of microorganisms in accelerating the growth rate of microorganisms and the comparison of the cultivation effect

[0035] Microbial material: Bacillus subtilis strain

[0036] Medium: cornstarch 15g / L, bean cake powder 10g / L, NH 42 SO 4 6g / L, corn steep liquor 3g / L, bran 5g / L, MgSO 4 1g / L, K 2 HPO 4 2g / L,MnSO 4 0.05g / L, CaCO 3 0.4g / L, pH7.0, 25% dissolved oxygen level, 42°C, 3 days.

[0037] Set a group of Bacillus subtilis cultured according to the conventional culture method; the operation of the stirred bioreactor adopting the microbial acoustic wave culture of the present invention is: first pass through the air inlet 14, feed steam into the empty tank of the bioreactor to sterilize. Then the sterilized culture medium is injected into the tank body 1 through the feed port 20 . When the temperature drops to the inoculation temperature, the liquid strain of Bacillus subtilis is inserted...

example 3

[0041] Example 3: Application of a stirred bioreactor for microbial sonic culture in increasing microbial oil production and comparison of production results

[0042] Microbial material: Rhodotorula viscosus CICC31596 strain

[0043] Medium: glucose 80g / L, ammonium sulfate 1.11g / L, potassium dihydrogen phosphate 2.5g / L, peptone 1.8g / L initial, pH 5.8, 40mL fermentation broth in 250mL Erlenmeyer flask.

[0044] The microorganism Rhodotorula viscosus CICC31596 bacterium is divided into two groups, and the first group adopts the traditional culture method to cultivate; the second group adopts the stirring type bioreactor of the present invention for microbial sonic cultivation to cultivate, first through the air inlet 13, Sterilize the empty tanks of the bioreactor by passing steam. Then the sterilized culture medium is injected into the tank body 1 through the feed port 16 . When the temperature drops to the inoculation temperature, the liquid strain of Bacillus coagulans is i...

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Abstract

The invention relates to an agitating type bioreactor for culturing microorganisms by sound waves. The bioreactor comprises a tank and an agitating device; the inner wall of the tank is provided with a sound generating mechanism; the sound generating mechanism comprises 4 signal generating units and 8 groups of sound generating rods arranged on the inner wall of the tank; each group of sound generating rods comprises an ultrasound generating rod and an audible sound generating rod; the 8 groups of sound generating rods are arranged in suitable positions on the inner wall of the tank; each signal generating unit is connected with two groups of sound generating rods through cables; the signal generating units generate high-frequency signals through MAX038-function sound generators, and the high-frequency signals drive the sound generating rods to generate sound after being amplified; the 8 groups of sound generating rods are arranged in different positions on the inner wall of the tank and can generate audible sound waves and ultrasound waves simultaneously, and the sound waves coordinate mutually to stimulate and culture the microorganisms in the tank so as to achieve an unexpected expect and greatly improve the microorganism fermentation efficiency. The bioreactor provided by the invention has good industrial application prospects.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a stirred bioreactor used for sonic culture of microorganisms. Background technique [0002] In terms of bioreactor design, traditional microbial fermentation conditions mainly consider factors such as medium composition, pH value, temperature, dissolved oxygen, ion concentration, and redox electricity. And the increase in the production of secondary metabolites has played a very large role in promoting. At present, studies have shown that sound waves can promote the growth of microorganisms, but there is no special fermentation culture bioreactor for sonic culture, especially no stirred bioreactor that can obtain ideal culture effects. Contents of the invention [0003] The purpose of the present invention is to provide a stirring bioreactor that can realize the optimal sonic culture of microorganisms and can promote growth, metabolism and synthesis by coupling acoustic effects on the basis of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12M1/02
CPCC12M27/02C12M35/04C12M41/12C12M41/24C12M41/40
Inventor 古绍彬吴影张永柱乔苏瑜李市场王大红孙建瑞
Owner HENAN UNIV OF SCI & TECH
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