136 results about "New Zealand white rabbit" patented technology

The invention discloses an antibody preparation method through which various organic phosphorus pesticide residues can be detected and the application, which belongs to the technical filed of agriculture. The antibody preparation method adopts the steps that firstly, O,O-diethylthiophosphoryl chloride is used to react with 4-aminobutyric acid to synthesize into general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP which comprises various organic phosphorus pesticide common structures-O, O-diethyl thiosulfate phosphoryl; secondly, the general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP is respectively coupled with bovine serum albumin BSA and chicken ovalbumin OVA by adopting carbodiimide method, to prepare general immunogen DENP-BSA and general coatingen DENP-OVA; and thirdly, multi-clonal antibody is prepared through immunized New Zealand white rabbits, the obtained antibody has obvious specificity to various organic phosphorus pesticides, such as thimet, demeton, disulfoion, omethoate, phoxim and quinalphos and can be used for the immune detection of the organophosphorus pesticides.

The invention discloses a diethoxy thiophosphate organophosphorus pesticide antibody and a preparation method and the application thereof. The antibody of the invention is prepared by introducing appropriate active arms to a diethoxy thiophosphate structure to prepare immunity hapten, coupling the hapten with bovine serum albumin to prepare immunity antigen and immunizing the originally immune New Zealand white rabbits; and a multi-residue ELISA detection method of the diethoxy thiophosphate organophosphorus pesticide is established with coatingen. Based on the detection method established by the antibody, more than 12 types of the diethoxy thiophosphate organophosphorus pesticide can be detected simultaneously, the limit of detection is 0.0002-3.938mug/ml and the detection method is suitable for on-site supervision of pesticide residues and rapid screening with high flux.

The invention discloses preparation and application of a tumor marker calreticulin detection kit, and belongs to the fields of genetic engineering and clinical diagnosis. A monoclonal antibody and a polyclonal antibody of the CRT (calreticulin) are prepared by recombinant CRT genetic immune BALB/c mice and new Zealand white rabbits; and an ELISA (enzyme-linked immunosorbent assay) kit with high specificity and sensitivity of which the lowest CRT content is 0.3125ng/ml and the detection limit is 40-0.3125ng/ml is built. The collected clinical sample is detected through the prepared ELISA kit; the result shows that CRT in blood serum of a patient with lung cancer is obviously higher than that in normal blood serum; significant statistic difference exists (P is smaller than 0.01); and an experimental method is provided for diagnosis of the clinical patients with lung cancer.

The invention discloses an amantadine artificial hapten and an artificial antigen as well as a preparation method and application of the amantadine artificial hapten and the artificial antigen. The molecular structural formula of the amantadine artificial hapten is as shown in the formula (I), and the molecular structural formula of the amantadine artificial antigen is as shown in the formula (II). The application is to utilize the amantadine artificial antigen to prepare an amantadine antibody. The amantadine artificial hapten disclosed by the invention retains the feature structure of amantadine to the largest extent, comprises active groups capable of being linked and coupled with carrier protein as an antigen determinant; the prepared and obtained amantadine artificial antigen can be immunized to obtain the amantadine antibody with high affinity, high sensitivity and high specificity; the titer of immune serum obtained by immunizing a New Zealand white rabbit is up to 1:70000; the amantadine artificial hapten and the artificial antigen can be used for quick and accurate immunodetection and immunoassay to amantadine.

The invention relates to model preparation, particularly to a rabbit portal vein thrombosis model which establishes a rabbit portal vein thrombosis model by a portal vein ligation catheterization method. The experimental animal adopts a New Zealand white rabbit; the experimental process comprises the steps of preoperative preparation: a preoperative experimental rabbit fasts all night and freely drinks water; anaesthetization; operation method: make the preoperative preparations including shaving feathers at the operating area after anaesthetization, disinfection and the like, enter the belly layer by layer, separate a portal vein, insert a 2Fr internal jugular vein tube by a catheterization method, ligate the portal vein using the tube as the inner support, suture and fix, lead to outside of body, and then conducting firm fixing. The model of the invention causes partial blood stasis, annular oppressed damage of blood vessel endothelium by ligating the portal vein, simultaneously carries out tube stimulation, starts intrinsic and extrinsic coagulation processes, increases the incidence of thromboembolism, and provides passages for the later partially collecting blood specimen, dynamic venogram, partial injection and the like; the first PVT occurrence time is about 30 min after the model is found via the portal vein radiography, and the model formation rate is higher than 75%.

The invention relates to a polyclonal antibody of dimethachlon and preparation and application thereof. The polyclonal antibody is prepared by the following steps: coupling hapten and bovine serum albumin BSA to prepare immune antigen; and immunizing New Zealand white rabbits. The polyclonal antibody is characterized in that: the hapten is dimethachlon-2-glutaric acid semi-ester; the dimethachlon-2-glutaric acid semi-ester is coupled with bovine serum albumin by an active ester method to synthesize the immune antigen; and the immune antigen is used for the New Zealand white rabbits so as to obtain the polyclonal antibody. The polyclonal antibody detects the residue of dimethachlon pesticide in a specimen by adopting an indirect competitive ELISA method; and envelope antigen used in the detection is a conjugate of the hapten and ovalbumin by the active ester method. The invention has the advantages that: the preparation method for the polyclonal antibody of the dimethachlon is simple; the polyclonal antibody can be used for quickly detecting the residue of the dimethachlon pesticide in the specimen; and compared with the prior equipmental analysis, the detection method has the advantages of simpleness, quickness, low detection cost, suitability for quickly screening a great number of samples, and the like.

The invention discloses a chlorothalonil antigen, an antibody preparation method and a residual chlorothalonil ELISA (Enzyme-Linked Lmmuno Sorbent Assay) detection method. Chlorothalonil is used as an initial reactant; an amino group is used for replacing a 4-bite chlorine atom; an artificial antigen is prepared by using a 1'1-carbonyl diimidazole method; a rabbit-resistant chlorothalonil polyclonal antibody can be obtained by an immune New Zealand white rabbit; the antiserum titer can be determined to be 1:5.12*10<4> by using the ELISA; and on the basis, the residual chlorothalonil ELISA detection method can be established; and the lowest detection concentration by using the method is 0.383ng/mL.

The invention discloses an Enzyme-linked immunosorbent assay (ELISA) method for egg allergen ovalbumin in foods, which belongs to the technical field of immunoassay. In the invention, a polyclonal antibody is obtained by immunizing a healthy New Zealand white rabbit with high-purity ovalbumin, and an indirect ELISA method for ovalbumin in foods is established by using the antibody as a detecting agent and the high-purity ovalbumin as a standard product and a coating antigen. In the invention, a quick and high-efficiency detection means is provided for detecting the ovalbumin content of the foods is provided; due to the adoption of the polyclonal antibody, the cost is low and the stability and the repeatability are high; the sensitivity is 1ppm, the linear range is 1 to 128ppm; and the high specificity and affinity of an immunoreactions ensure that the ELISA has extremely high selectivity and sensitivity.

The invention relates to a competitive inhibition enzyme-linked immunoassay (ELISA) method of grass carp interleukin 10 (IL-10). The method comprises the following steps of: immunizing a New Zealand white rabbit by utilizing grass carp interleukin 10 proteins which is expressed in a recombined way as an immunogen to obtain a polyclonal antibody; marking the antibody by utilizing horseradish peroxidase; and finally, establishing the competitive inhibition ELISA method by taking the recombined grass carp interleukin IL-10 protein as a covered antigen, wherein the competitive inhibition ELISA method can be used for the protection detection of the grass carp interleukin 10. The detection range of the ELISA method is 0.1-100 ng/mL. By utilizing the polyclonal antibody and the competitive inhibition ELISA method, the cost is low, and the stability and the repeatability are good. The conventional method is that the competitive inhibition ELISA method is firstly established to detect protection expression of the grass carp interleukin 10.

The invention discloses a preparing method and a detecting method for MRJR1 antibody pairs in honey and a kit. The amino acid sequence of the specific polypeptide M4 of MRJR1 is IKEALPHVPIFD, the IKEALPHVPIFD is shown in SEQ ID NO:1; the amino acid sequence of the specific polypeptide M5 of the MRJR1 is SGEYDYKNNYPSDID, and the SGEYDYKNNYPSDID is shown in SEQ ID NO:2; New Zealand white rabbits are immunized. An ELLSA double-antibody sandwich method includes the following steps that a caught antibody SP-1 is coated, sealed and washed, an antigen is added, an enzyme-labeled detecting antibody HRP-SP-2 of an is added, washing is carried out, a color rendering substrate is added, a color rendering stopping solution is added, and the light absorption value of 450 nm-630 nm of a sample is measured through an enzyme-labeling instrument. The antigen is to-be-detected honey or pure honey of a known honey source, and the quality of the to-be-detected honey is judged by comparing the light absorption value of the MRJR1 of the to-be-detected honey and the light absorption value of the MRJR1 of the pure honey of the known honey source. According to the preparing method, the detecting method and the kit, a new reliable rapid detecting method is provided for the quality of the honey and detection of adulteration amount, and the detecting method can be used for honey-product-quality control of honey-product-quality supervision departments and honey-product-quality control of trade processing enterprises.

The invention belongs to a preparation method of an antigen and an antibody, which is specially used for recognizing the specificity of inedible pigment basic orange and developing a rapid test immunoassay kit for the basic orange. The invention includes the synthesis of basic orange artificial antigen and a preparation method for an antibody thereof, which mainly comprises: taking glutaric dialdehyde as a coupling agent to synthesize an immunizing antigen and an envelope antigen respectively with bovine serum albumin and ovalbumin, injecting the immunizing antigen to a New Zealand white rabbit to prepare the basic orange antibody with high titer. The antibody has good stability and high titer, and solves the technical difficulty for developing the basic orange enzyme-linked immunoassay kit. The method has the advantages of strong practicality, no need of expensive equipment in the preparation process of the antigen and the antibody and strong operability, thus being suitable for large-scale production.

The invention provides an immunogen for obtaining an Nrf1D protein antibody, the Nrf1D protein antibody and an Elisa detection kit. The immunogen is obtained as follows: polypeptide with the amino acid sequence shown in SEQ ID NO.1 is synthesized firstly, Sulfo-SMCC is used as a coupling agent, the polypeptide and KLH (keyhole limpet hemocyanin) are coupled; the antibody is obtained as follows: immunogen is taken as an antigen, the antigen is emulsified with FCA (freund's complete adjuvant) to immunize a New Zealand white rabbit, blood of the immunized New Zealand white rabbit is centrifuged, and a supernatant after centrifugation is collected; the Elisa detection kit comprises components including an ELISA plate, an enveloping solution, a blocking solution, an ELISA secondary antibody, a TMB color developing solution, a stop solution, a detection antigen and the Nrf1D protein antibody, the detection antigen is the polypeptide with the amino acid sequence shown in SEQ ID NO.1, glutaradehyde is used as a coupling agent, and the polypeptide and BSA bovine serum albumin are coupled.

The invention relates to the field of biotechnology and aims at providing an application of AIF1 (Allograft Inflammatory Factor 1) protein and antibody to preparing a southern oyster anti-infectious immune preparation. The amino acid sequence of the protein is shown in SEQ ID NO.2; the open reading frame nucleotide sequence of the protein is shown in SEQ ID NO.1; and the application of the protein to preparing the southern oyster anti-infectious immune preparation is as follows: immunizing a New Zealand white rabbit by use of the protein so as to obtain polyclonal antiserum. According to the invention, the open reading frame complete sequence of a southern oyster AIF1 gene is obtained; and by virtue of constructing a prokaryotic expression vector, a soluble AIF1 protein product is expressed and purified, and the polyclonal antiserum is prepared; and the polyclonal antiserum has an effect of restraining inflammatory reactions caused by a southern oyster pathogeny RLO (Rickettsia-Like Organism) and gram-negative bacterium LPS (Lipopolysaccharide), as well as cytoclasis and apoptosis.

The invention belongs to a method for preparing antigens and antibodies, which is particularly used for the specific recognition of inedible pigment rhodamine B and the development of a speed-measuring immune reagent kit of the rhodamine B. The method comprises the synthesis of an artificial antigen of the rhodamine B and a method for preparing an antibody of the rhodamine B. Rhodamine 123 substitutes the rhodamine B to synthesize an immune antigen and cover the antigen, and injects the immune antigen into the body of a New Zealand white rabbit to prepare the high titer antibody of the rhodamine B, and the antibody has good stability and high titer, and solves technical difficulties for the development of an nzyme-linked immune reagent kit of the rhodamine B. The method has strong practicability, does not need high-value equipment during the preparation process of the antigen and the antibody, has strong maneuverability, and is suitable for mass production.

The invention relates to a methoxy-organophosphorus pesticide broad-spectrum specific polyclonal antibody and the application; the invention is characterized in that the antibody is obtained through the following steps: phosphate is taken as the semi-antigen and coupled with bovine serum albumin BSA to prepare an antigen, and immunise New Zealand white rabbits to prepare the polyclonal antibody. The polyclonal antibody is used to detect the methoxy-organophosphorus pesticide residue through indirect competitive ELISA; the coating antigen used in the detection is a conjugate between hapten and OVA through active ester or mixed anhydride method. The methoxy-organophosphorus pesticide broad-spectrum specific polyclonal antibody has the advantages that the broad-spectrum specific antibody can detect a variety of methoxy-organophosphorus pesticide in vegetables and other foods at the same time so as to significantly save the sample testing time and cost, thereby fully demonstrating the rapid and low-cost characteristics of immunoassay technology.

The invention discloses an ELISA (Enzyme Linked Immunosorbent Assay) test kit for testing difenoconazole residue and a test method. Chloroethane is utilized to substitute hydrogen atoms of three sites on a benzene ring in a difenoconazole molecular structure with ethyl groups, which are oxidized into carboxyl groups, afterwards, the mixed anhydride method is utilized to connect a hapten onto a carrier, so that an artificial antibody is formed, the carrier is bovine serum albumin, an artificial difenoconazole antigen is utilized to immunize New Zealand white rabbits to prepare an antibody, and along with related agents, such as standard difenoconazole, washing concentrate, substrate color-developing solution and stop solution, the antibodies compose the ELISA test kit. The main steps of a test include: sample preprocessing, kit testing, and test result analysis. Compared with a variety of current conventional test methods applied widely, the enzyme linked immunosorbent assay method disclosed by the invention has advantages, such as low cost and high testing sensitivity, and an effective method is provided for difenoconazole residue testing.

The invention discloses a novel anti-staphylococcus aureus polyclonal antibody for treating staphylococcus aureus infection. The antibody is obtained by immunizing a New Zealand white rabbit with staphylococcus aureus MntC protein as a major immunizing antigen. The novel anti-staphylococcus aureus polyclonal antibody has an immune regulation effect and is capable of promoting the phagocytosis of the organism to the staphylococcus aureus so as to achieve the effect of treating the staphylococcus aureus infection.

The invention discloses a coupling matter of terbutaline in general formula (I), constituted in coupling by terbutaline hapten and carrier matter generating immunogenicity, which is bovine serum albumin or ovalbumin. Whereinto, n is the number of terbutaline molecules combined with a bovine serum albumin molecule. Said n is integer from 1 to 15. cBSA is activated bovine serum albumin and the range of molecular weight is 66KDa-69KDa. The invention also discloses the preparation method of said coupling matter. Terbutaline is connected with carrier matter generating immunogenicity to combine the coupling matter which can induce animal immune system to generate antibody. By immunizing New Zealand white rabbit the coupling matter of terbutaline in invention prepares antiserum with value of more than 1:50,000. The lowest measuring limitation is 0.3ppb and IC50 is 9.25ppb. The invention is convenient, quick, special and accurate. It provides a foundation for preparing ELISA kit for detecting of terbutaline.

The invention provides a meat rabbit breeding method. The meat rabbit breeding method comprises the following steps: (1) selecting a New Zealand white rabbit as a male parent and a California rabbit as a female parent, and carrying out artificial insemination to obtain a first-filial generation G1; (2) selecting a female rabbit of the first filial generation G1 obtained in the step (1) as a femaleparent and a chekered giant rabbit as a male parent, and carrying out artificial insemination to obtain a second-filial generation G2; (3) backcrossing the hybridized second-filial generation G2 obtained in the step (2) with a New Zealand white rabbit to obtain a hybridized third-filial generation G3; and (4) selecting high-quality meat rabbits from the third-filial generation G3 obtained in thestep (3), and performing cross fixation to obtain high-generation hybrid meat rabbits. The new variety has the advantages of three rabbits, is high in growth speed, high in meat yield, delicious in meat, obviously enhanced in epidemic disease resistance and low in feed requirement, and the feeding cost can be reduced.

The invention provides a brachypodium distachyon functional centromere histone CENH3 and application. Firstly, the nucleotide sequence of a brachypodium distachyon functional centromere histone CENH3 gene is predicted through the bioinformatics sequence comparison method, a polypeptide is synthesized according to a segment of specific sequence of the N end of an amino acid sequence encoded by the nucleotide sequence, New Zealand white rabbits are conventionally immuned through the polypeptide, antiserums are prepared, and a CENH3 protein antibody is obtained through antigen affinity purification. The CENH3 antibody can be used for immunostaining experiments, physical positioning of centromere and chromatin immunoprecipitation (ChIP) experiments, the functional DNA sequence of a brachypodium distachyon functional centromere is obtained, positioning of centromere genome genetic maps and physical maps is carried out, and important tools and information are provided for research of centromere formation and function exercise mechanisms and building of artificial chromosomes.

The invention provides pineapple function centromere antigenic polypeptide and application thereof, an amino acid sequence is as shown in SEQ ID NO.2, one polypeptide is synthesized according to a specific sequence at an N terminal of the amino acid sequence, a New Zealand white rabbit is conventionally immunized by using the polypeptide, antiserum is prepared, an antibody of CENH3 protein is obtained by affinity purification of an antigen, an immunofluorescence test of the antibody is performed, the antibody of the pineapple CENH3 protein can be used for cytological localization of position of pineapple function centromere, also can be used for ChIP-seq combined with a chromatin immunoprecipitation technology of the pineapple, the position, size and sequence information of function centromere on chromosome of the pineapple are determined, the location of a centromere genome genetic map and a physical map is performed, and important tools and information are provided for formation of the centromere and study of a function performing mechanism and the construction of artificial chromosome.

The invention discloses a valnemulin artificial antigen and a preparation method and application thereof. The valnemulin artificial antigen is a compound as shown in a formula I, wherein n denotes a natural number from 1 to 25; and K denotes carrier protein. When the artificial antigen is used to immunize a New Zealand white rabbit, an antibody with specific reaction to valnemulin can be obtained. According to ELISA experiments, the titer of the obtained antiserum reaches 1:102400. The invention provides a wide application and development space for the development of detection reagent used in the enzyme-linked immunoassay method of the valnemulin and the preparation of the enzyme-linked immunoassay reagent kit of the valnemulin. When the antibody obtained by using the artificial antigen is used for the detection of the valnemulin, the operation is simple and convenient, the specificity is good and the accuracy is high. The antibody can be used for the rapid and high-efficiency screening of the valnemulin in animal-based foods and a technical foundation is laid for the rapid detection of the valnemulin. The formula I is as shown is the accompanying drawing.

The invention discloses a stress phosphorylation antibody aiming at a human Tudor-SN protein T103 site and a preparation method thereof. The preparation method adopts a multifunctional human Tudor-SN protein as a research object and comprises the following steps of determining if threonine at a 103 site of a human Tudor-SN protein under external environment oxidation stress is subjected to phosphorylation modification by near-infrared double-color laser imaging and mass spectrometry, synthesizing a T103 phosphorylation site-containing semiantigen polypeptide according to the secondary structure prediction result, coupling the T103 phosphorylation site-containing semiantigen polypeptide and a carrier protein (KLH) into a complete antigen, carrying out immunization on SPF-level New Zealand white rabbit by the complete antigen combined with an adjuvant four times and collecting, purifying and identifying a T103 specific stress phosphorylation polyclonal antibody. In practical application, Tudor-SN protein epigenetic phosphorylation modification in different stress environments can be detected, the effect mechanism of the Tudor-SN protein epigenetic phosphorylation modification in cell stress tumor propagation and migration can be discussed and a latent action target point for clinical tumor disease diagnosis and treatment is provided.