Amantadine artificial hapten and artificial antigen as well as preparation method and application of amantadine artificial hapten and artificial antigen

An artificial hapten, amantadine technology, applied in the preparation of carboxylic acid amides, chemical instruments and methods, preparation of organic compounds, etc., can solve the problems of artificial hapten and artificial antigen without amantadine, and achieve immunogen Good sex, strong specificity, good specificity effect

Active Publication Date: 2015-03-11
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the prior art, there are no related reports

Method used

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  • Amantadine artificial hapten and artificial antigen as well as preparation method and application of amantadine artificial hapten and artificial antigen
  • Amantadine artificial hapten and artificial antigen as well as preparation method and application of amantadine artificial hapten and artificial antigen
  • Amantadine artificial hapten and artificial antigen as well as preparation method and application of amantadine artificial hapten and artificial antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This implements a kind of preparation method of amantadine artificial antigen (reaction process is as follows: figure 1 ), including the following steps:

[0056] (1) Preparation of artificial hapten:

[0057] ① Weigh 200mg of amantadine (1.322mmol), 198mg of succinic anhydride (1.980mmol) in a 100ml single-necked round bottom flask, add 10ml of pyridine, add a stirrer, heat the oil bath to 100°C and stir under reflux for 16 hours; After the end, the solvent was evaporated to dryness under reduced pressure, the residue was dissolved with 15ml of double distilled water, the aqueous solution was extracted with 3×15ml of dichloromethane, the organic phases were combined and washed with 15ml of double distilled water, the organic phase was dried over anhydrous magnesium sulfate, filtered, and transferred to After drying, 252 mg of product A was obtained as a colorless oil;

[0058] The colorless oily product A is detected by TLC, the chromatographic solution is an aqueous...

Embodiment 2

[0075] This implements a kind of preparation method of amantadine artificial antigen (reaction process is as follows: figure 1 ), including the following steps:

[0076] (1) Preparation of artificial hapten:

[0077] ① Weigh 200mg of amantadine (1.322mmol) and 145.6mg of succinic anhydride (1.455mmol) into a 100ml single-necked round bottom flask, add 10ml of pyridine, add a stirrer, heat the oil bath to 105°C and stir under reflux for 17 hours; After the reaction was completed, the solvent was evaporated to dryness under reduced pressure, the residue was dissolved with 15ml of double distilled water, the aqueous solution was extracted with 3×15ml of dichloromethane, the organic phases were combined and washed with 15ml of double distilled water, the organic phase was dried over anhydrous magnesium sulfate, filtered, After drying, 251.5 mg of product A was obtained as a colorless oil;

[0078] The colorless oily product A is detected by TLC, and the chromatographic solution ...

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Abstract

The invention discloses an amantadine artificial hapten and an artificial antigen as well as a preparation method and application of the amantadine artificial hapten and the artificial antigen. The molecular structural formula of the amantadine artificial hapten is as shown in the formula (I), and the molecular structural formula of the amantadine artificial antigen is as shown in the formula (II). The application is to utilize the amantadine artificial antigen to prepare an amantadine antibody. The amantadine artificial hapten disclosed by the invention retains the feature structure of amantadine to the largest extent, comprises active groups capable of being linked and coupled with carrier protein as an antigen determinant; the prepared and obtained amantadine artificial antigen can be immunized to obtain the amantadine antibody with high affinity, high sensitivity and high specificity; the titer of immune serum obtained by immunizing a New Zealand white rabbit is up to 1:70000; the amantadine artificial hapten and the artificial antigen can be used for quick and accurate immunodetection and immunoassay to amantadine.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to an amantadine artificial hapten, artificial antigen and a preparation method and application thereof. Background technique [0002] Amantadine can inhibit the virus from penetrating into the host cell, affect the uncoating of the virus, and inhibit its reproduction. It is clinically used to prevent and treat human influenza virus infection. It has the characteristics of low price and obvious drug effect. Drugs for the treatment of Parkinson's disease. However, the accumulation of amantadine can cause various adverse reactions such as drowsiness, insomnia, dizziness, depression, nausea, and loss of appetite to the human body, and has certain toxic and side effects. Long-term use of amantadine can also cause the virus to develop drug resistance to varying degrees and induce new drug-resistant viruses. [0003] However, in November 2012, the media revealed...

Claims

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Application Information

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IPC IPC(8): C07C233/47C07C231/02C07K14/47C07K16/44G01N33/53
Inventor 杨华邵越水张巧艳钱鸣蓉汪建妹吴俐勤肖英平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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