10results about How to "Conformational stability" patented technology

The invention relates to a mutant of an RSV pre-fusion conformation F protein, the mutant comprises amino acid mutation relative to an amino acid sequence of a wild type RSV F protein, the amino acid mutation is that amino acids at positions 392 and 493 or near the 493 are substituted by C, and a disulfide bond is formed between mutated positions 392C and 493C or near the 493C. The invention also provides a related DNA (deoxyribonucleic acid) coding sequence, a recombinant plasmid, an mRNA (messenger ribonucleic acid) sequence and a corresponding RSV vaccine. By introducing disulfide bonds at proper positions and combining with cavity filling, electrostatic mutation, proline mutation and the like, the expression and stability of the F protein with conformations before and after fusion and trimerization conformations are remarkably improved, and the F protein is remarkably inhibited from being converted into conformations after fusion. The mutant is also applied to preparation of RSV vaccines and detection of respiratory syncytial virus infection.

The invention provides collagen modified superoxide dismutase and a preparation method and application thereof, the preparation method comprises the following steps: dissolving collagen in water, sequentially adding superoxide dismutase, EDC and NHC into the water, and stirring in a water bath heat preservation state to obtain a flowable gel sample; diluting the obtained gel sample with water, centrifuging, and collecting a lower-layer viscous liquid; and adding the centrifuged viscous liquid into a PBS (phosphate buffer solution), and dialyzing to obtain the collagen modified superoxide dismutase. Trehalose and mannitol are added into the collagen modified superoxide dismutase, the mixture is stirred and mixed evenly, the mixture is poured into a mold to be freeze-dried and extruded to be flat, and the repairing patch is obtained. The crosslinking modified superoxide dismutase obtained in the invention has good stability, and the obtained repair patch is convenient to use, can promote wound healing, and has wide application prospects and practical significance.

The invention belongs to the field of vaccines, and provides an adjuvant composition containing MF59 and CpG. The invention discloses a full-liquid preparation RSV vaccine containing the adjuvant composition. The vaccine contains RSV pre-F protein. The preparation method of the all-liquid preparation RSV vaccine comprises the operation of carrying out compatibility on the RSV pre-F protein and the adjuvant composition. According to the adjuvant composition and the full-liquid preparation RSV vaccine based on the adjuvant composition, the conformation of pre-F protein before fusion can be kept stable in a liquid dosage form, the preparation cost of the vaccine can be greatly reduced, and meanwhile the cellular immune effect and the humoral immune effect of the vaccine are improved.

The invention relates to the technical field of coatings, and discloses a quaternary ammonium salt-attapulgite modified epoxy acrylic resin and a quaternized imidazoline type corrosion inhibitor which can show good corrosion inhibition performance when metal is eroded. When the corrosion inhibitor is used, carbon-nitrogen double bonds on an imidazoline ring and lone pair electrons on nitrogen atoms generate corresponding covalent adsorption with a metal surface, after the corrosion inhibitor is grafted with the attapulgite, agglomeration is avoided, fine particles of the nano attapulgite are filled into matrix network gaps, so that the viscosity of a system is increased, the compatibility between the corrosion inhibitor and a matrix is enhanced, and the corrosion inhibitor has good corrosion resistance. When the corrosion inhibitor is applied to metal, a layer of protective film is formed on the surface of the metal to improve the mechanical property, a long-chain alkane chain in the corrosion inhibitor has hydrophobicity, a hydrophobic layer is formed on the surface of the metal to hinder migration of corrosion reactants, the corrosion resistance of compounds is promoted, and the modified coating has excellent corrosion resistance and mechanical property.

The invention discloses an RSV pre-fusion F protein mutant, nucleic acid and an mRNA vaccine. The mutant is obtained by mutating the full length or partial region of a wild type RSV pre-fusion F protein. The RSV pre-fusion F protein mutant disclosed by the invention can ensure stable conformation before fusion, and is beneficial to inducing generation of a higher-level neutralizing antibody; the respiratory syncytial virus mRNA vaccine can effectively induce generation of a neutralizing antibody aiming at RSV pre-fusion F protein, and after being immunized by the respiratory syncytial virus mRNA vaccine, a host can generate extremely high respiratory syncytial virus F protein specific T cell immune response; the antigen compatibility of the combined mRNA vaccine is good, and high titer aiming at the respiratory syncytial virus and the new crown mutant strain EG5.1 can be realized at the same time.

The present invention discloses an aromatic amine compound and an organic electroluminescent device comprising the same. The structure of the aromatic amine compound is shown in the general formula (1): The compound of the present invention has excellent hole transport ability and exciton blocking ability. When the aromatic amine compound of the present invention is used to form the light-emitting layer auxiliary layer material of the organic electroluminescent device, the device efficiency can be improved and the life can be extended at the same time, especially the device efficiency can be improved.

The invention relates to the technical field of bio-enzyme catalysts, in particular to a bio-enzyme catalyst for preparing mussel mucin and a preparation method of the bio-enzyme catalyst, and the preparation method comprises the following steps: S1, adding N-isopropylacrylamide and vinyl imidazole into a reactor, adding azodiisobutyronitrile, and reacting to obtain a copolymer; s2, adding chitin into concentrated sulfuric acid, dropwise adding fuming sulfuric acid, and reacting to obtain a chitin derivative; and S3, adding transglutaminase and tyrosinase into a phosphate buffer solution, adding the copolymer and the chitin derivative, adjusting reaction conditions, stirring at room temperature, standing, centrifuging or filtering and separating to obtain the bio-enzyme catalyst. The bio-enzyme catalyst prepared by the invention can improve the purification efficiency and dopa content in the preparation of the mussel mucin, and promotes the efficient purification of the recombinant mussel mucin.

This invention provides a modified hemagglutinin (HA) protein for preparing a broad-spectrum vaccine against H9 avian influenza, a broad-spectrum vaccine based on the HA protein, a preparation method, and applications. The modified HA protein is obtained through the following strategy: consensus sequences are constructed as immunogenic backbones for the HA sequences of H9 avian influenza viruses at different epidemic periods; based on this, two types of functional mutations are directionally introduced: one type involves transplanting key epitope residues targeted by known broad-spectrum antibodies, and the other type involves neutralizing epitope residues predicted based on viral evolutionary patterns. The modified HA protein of this invention can maintain a highly similar overall fold and trimer interface to the wild type, can be efficiently expressed in eukaryotic systems, and can induce high levels of neutralizing antibodies with broad-spectrum cross-protective effects in target animals. It provides a core antigen candidate for the development of a new generation of H9 broad-spectrum vaccines and has broad application prospects.

The invention discloses F protein with stable pre-fusion conformation, nano-lipid particles and application of the F protein and the nano-lipid particles. The amino acid sequence of the respiratory syncytial virus F protein is subjected to point mutation, so that the F protein is stabilized at the F protein before fusion, the expression level of the obtained mutant F protein is high, and the humoral immunity and cellular immunity response level of a vaccine in an animal body is further improved.

The invention discloses a photochromic rare earth helicoid as well as a preparation method and application thereof, belongs to the technical field of photochromic rare earth complex materials, and particularly relates to a photochromic rare earth helicoid as well as a preparation method and application thereof. The invention aims to solve the problems that the existing anti-counterfeiting technology is easy to decode, the production period is long and the cost is high. A supramolecular spiral body constructed by four-strand ligands and metal ions is innovatively designed and synthesized, and the spiral body can effectively solve the problem that the molecular motion space of an off-color material is limited in a solid state, so that the off-color performance of the off-color material in a film-forming material is improved. The spiral structure of the photochromic rare earth spiral body has flexibility and variability, so that the conformation of the material can be rapidly adjusted when the material is subjected to photostimulation, and therefore, rapid color change of the photochromic rare earth spiral body material is realized, and the color generation rate of the photochromic rare earth spiral body material in a strong light environment is 1t; 3s. In addition, the synthesis method of the photochromic rare earth supramolecular spiral body is simple and convenient, the synthesis cost is low, and the possibility of large-scale industrial application is achieved.