Simultaneous imprinting of polymer microspheres with various protein peptides and its preparation and application

Abstract

The invention relates to a molecular imprinting microsphere material for selective recognition of multiple target proteins and a preparation process of molecular imprinting microsphere material. According to the molecular imprinting microsphere material, specific peptide fragments corresponding to the multiple target proteins are used as template molecules, a high-molecular polymer is used as a matrix, and the molecular imprinting microsphere material is prepared by a polymer phase inversion method. The molecular imprinting microsphere material is used for selective recognition of standard target proteins.

Description

technical field [0001] The invention belongs to the preparation technology of polymer materials and its application in protein recognition, and specifically relates to a reverse molecular imprinted microsphere material based on polyprotein peptide segments. Background technique [0002] Molecular imprinting technology is a technology for preparing materials with high selective recognition ability for target molecules by imitating the molecular recognition process in the life process and using artificial synthesis methods. Since Klaus Mosbach et al. published a report on the synthesis of theophylline molecularly imprinted polymers using non-covalent bonds in "Nature" in 1993, molecularly imprinted materials, as a synthetic highly selective material, have rapidly entered the field of vision of many scientists around the world. At present, great progress has been made in the imprinting of small organic molecules and metal ions, and the experimental methods and evaluation standa...

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Problems solved by technology

Since the polymerization reaction often involves conditions such as heat, ultraviolet light, and strong redox reactions that easily denature protein molecules, the specific recognition of imprinted polymers is reduced.

In addition, proteins are often used as template molecules in these blots, but because protein template molecules are difficult to obtain and expensive, the use of western blotting materials is limited (Sellergren, B. Nature Chemistry, 2, 2010, 7)

[0003] At the same time, for the analysis and detection of multi-target protein systems, single-template molecular imprinting materials that can only recognize a single protein can no longer meet the needs of multi-protein simultaneous detection, so it is necessary to prepare multi-protein recognition materials that can simultaneously recognize multiple target proteins

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