Preparation and application of adipose-derived stem cell exosome in facial rejuvenation

An adipose stem cell and exosome technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as low fat survival rate

Inactive Publication Date: 2018-08-07
王丽丽
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the fat survival rate is low, and the effect can only be seen after multiple operations

Method used

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Embodiment 1

[0052]A preparation and application of adipose stem cell exosomes in facial rejuvenation, characterized in that: it includes adipose stem cell separation, exosome extraction and adipose stem cell exosome-nano-collagen coating;

[0053] The fat stem cell separation comprises the following steps:

[0054] S1. Take the adipose tissue from the groin of New Zealand white rabbits, put it into Duchenne's phosphate buffer solution containing penicillin and streptomycin to wash away the blood stains, transfer it to a new petri dish, shred it fully, add digestive juice, and pipette Stir well until the tissue can enter and exit the suction nozzle smoothly;

[0055] Wherein, the digestive juice includes 10% fetal bovine serum, 0.09% collagenase I and the rest is DMEM / F12 in terms of mass percentage.

[0056] S2. Transfer the adipose tissue into a centrifuge tube, digest at 37°C for 1 hour, and shake regularly to fully mix the tissue and digestive juice. After digestion, repeatedly pipett...

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Abstract

The invention provides a preparation and application of an adipose-derived stem cell exosome in facial rejuvenation. The preparation method comprises the following steps: separation of adipose-derivedstem cell, extraction of the exosome and coating of adipose-derived stem cell exosome-nano collagen. By carrying out facial microscopic carving and face-lifting by virtue of the adipose-derived stemcell exosome-nano collagen, the superiorities of the adipose-derived stem cell exosome-nano collagen, compared with adipose-derived stem cell autoplastic transplantation, exosome injection or pure nano collagen, in skin reconstructive repairing and micro-face lifting are observed. Particularly, adipose-derived stem cells are separated, the exosomes of the adipose-derived stem cells are extracted,the nano collagen is fused with the separated exosome to form an adipose-derived stem cell exosome-nano collagen combined preparation, the combined preparation is injected into the skin an injured part of a New Zealand white rabbit, the promotion effect of the adipose-derived stem cell exosome-nano collagen, compared with a pure adipose-derived stem cell transplantation group, an exosome group andpure nano collagen, in the regeneration of injured skin is observed, and meanwhile, a user can observe whether the adipose-derived stem cell exosome is harmful to a human body.

Description

technical field [0001] The invention relates to the field of cosmetic plastic surgery, in particular to the preparation and application of adipose stem cell exosomes in facial rejuvenation. Background technique [0002] Traditional autologous fat filling is just to inject purified autologous fat into the desired parts of the face. However, the fat survival rate is low, and the effect can only be seen after multiple operations. Fat is prone to necrosis or hollowness, which makes the face look uneven, and more and more studies have found that it is not fat stem cells that really have a therapeutic effect, but the exosomes secreted by them are achieved through the regulation of the local tissue microenvironment. "Nano Rejuvenating Face" breaks through the purification technology of autologous adipose stem cells. The purified high-concentration autologous adipose stem cells have a high regeneration ability and are used in the field of facial micro-sculpture. For women with unc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61L27/38A61L27/24A61L27/50A61L27/60
CPCA61L27/24A61L27/3834A61L27/50A61L27/60A61L2400/06A61L2400/12A61L2430/40C12N5/0667C12N2509/10
Inventor 王丽丽
Owner 王丽丽
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