Application of AIF1 (Allograft Inflammatory Factor 1) protein and antibody to preparing southern oyster anti-infectious immune preparation

Abstract

The invention relates to the field of biotechnology and aims at providing an application of AIF1 (Allograft Inflammatory Factor 1) protein and antibody to preparing a southern oyster anti-infectious immune preparation. The amino acid sequence of the protein is shown in SEQ ID NO.2; the open reading frame nucleotide sequence of the protein is shown in SEQ ID NO.1; and the application of the protein to preparing the southern oyster anti-infectious immune preparation is as follows: immunizing a New Zealand white rabbit by use of the protein so as to obtain polyclonal antiserum. According to the invention, the open reading frame complete sequence of a southern oyster AIF1 gene is obtained; and by virtue of constructing a prokaryotic expression vector, a soluble AIF1 protein product is expressed and purified, and the polyclonal antiserum is prepared; and the polyclonal antiserum has an effect of restraining inflammatory reactions caused by a southern oyster pathogeny RLO (Rickettsia-Like Organism) and gram-negative bacterium LPS (Lipopolysaccharide), as well as cytoclasis and apoptosis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the effect of AIF1 gene, protein and antibody in preparing oyster anti-infection immune preparation. Background technique [0002] Allograft inflammatory factor AIF-1 (Allograft inflammatory factor-1) is a calcium-binding protein with a molecular weight of about 17kD that can be induced by interferon-γ. At present, AIF1 has been reported in many species, its amino acid sequence is highly conserved, and its structure contains a conserved EF-hand domain. Studies have proved that AIF1 is an immune regulatory factor in the process of macrophage activation, which can promote the proliferation and migration of macrophages, and has multiple biological functions, participating in the body's immune rejection, immune inflammatory response, auto-inflammatory and non-inflammatory damage , and is associated with the occurrence and development of many diseases. [0003] Oyster is an important econ...

AI Technical Summary

Problems solved by technology

The rapid development of oyster aquaculture has made important contributions to my country's agricultural economy and greatly improved the living standards of coastal fishermen. The limitations of the farming model and the frequent occurrence of oyster farming diseases have caused huge economic losses, greatly damaged the enthusiasm of local fishermen, and limited the further development of the oyster industry

Images