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Application of chitosan oligosaccharide in improving lower limb ischemia caused by various diseases

A technology of chitosan oligosaccharides and diseases, applied in the field of pharmacy, can solve the problems of less research on lower limb ischemia and no patent reports, and achieve the effects of promoting capillary neogenesis, inhibiting tissue ulcers, and promoting expression

Active Publication Date: 2019-09-03
HUBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a functional food, chitosan oligosaccharide has good effects in anti-oxidation and anti-inflammation, and has the advantages of good water solubility, easy absorption and utilization, and no toxic and side effects. It has a very wide range of applications and development prospects. However, chitosan There are few related studies on oligosaccharides in lower extremity ischemia, and no related patents have been reported

Method used

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  • Application of chitosan oligosaccharide in improving lower limb ischemia caused by various diseases
  • Application of chitosan oligosaccharide in improving lower limb ischemia caused by various diseases
  • Application of chitosan oligosaccharide in improving lower limb ischemia caused by various diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Oligochitosan improves blood perfusion and promotes capillary regeneration in mice with acute lower limb ischemia

[0030] In this embodiment, in order to evaluate the regulating effect of oligochitosan on blood perfusion and capillary regeneration of lower limb ischemia mice, 24 four-week-old C57BL / 6 mice were randomly divided into 4 groups, 6 in each group, Corresponding to sham operation group (Control), lower limb ischemia group (Ischemia), sham operation and chitooligosaccharide combined group (COS), lower limb ischemia and chitooligosaccharide combined group (Ischemia-COS). Mice were raised in a constant environment of 23±2°C and 50±5%. After the environmental adaptation period, the sham operation group was given water after sham operation, and the lower limb ischemia group was given water after femoral artery ligation, sham operation and chitosan oligosaccharide The combination group was given chitosan oligosaccharide after sham operation, and the lower limb isch...

Embodiment 2

[0037] Oligochitosan regulates lower extremity ischemia induced by diabetes mellitus

[0038] In this example, in order to evaluate the regulatory effect of chitosan on lower limb ischemia mice caused by diabetes, 24 four-week-old C57BL / 6 mice were randomly divided into 4 groups, 6 in each group, corresponding to the sham operation group (Control), lower extremity ischemia group (Ischemia), diabetic lower extremity ischemia group (HFD-STZ-Ischemia), diabetic lower extremity ischemia plus chitooligosaccharide group (HFD-STZ-Ischemia-COS).

[0039] The mice were raised in an environment with constant temperature and humidity. After the environmental adaptation period, 12 mice in the diabetes group were fed with high-fat diet for 1 month, and at the same time, they were injected intraperitoneally with 85 mg / kg of streptozotocin (STZ) for one week to induce diabetes. model; while 12 rats in the normal group were fed with normal feed for 1 month. Mice with measured random blood gl...

Embodiment 3

[0044] Oligochitosan Alleviates APoE - / - Lower limb ischemia induced by atherosclerosis in mice

[0045] This embodiment is to evaluate chitosan oligosaccharide for APoE - / - Regulation of lower limb ischemia induced by atherosclerosis in mice.

[0046] Take 12 four-week-old wild-type C57BL / 6 mice and 12 four-week-old APoE mice - / - Mice were raised in an environment with constant temperature and humidity. After the environmental adaptation period, APoE - / - The 12 mice in the control group were fed with high-fat diet for 2 months to induce atherosclerosis model, and the 12 mice in the control group were fed with normal diet for 2 months.

[0047] According to the atherosclerosis index to determine the success of the modeling, the corresponding sham operation and femoral artery ligation were performed respectively. In the control group, 12 wild-type C57BL / 6 mice were randomly divided into 2 groups, 6 mice in each group, one group was sham operation, the other was Group arteri...

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Abstract

The invention discloses an application of chitosan oligosaccharide in improving lower limb ischemia caused by various diseases. The animal experiments show that chitosan oligosaccharide can restore blood perfusion of ischemic animals in lower limbs, promotes capillary angiogenesis, and inhibits the local inflammatory response. The application has positive value for improving limb ischemia, gangrene and inflammation caused by clinical lower limb ischemia.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and relates to the application of chitosan oligosaccharide in lower limb ischemia caused by various diseases. Background technique [0002] Lower extremity ischemia is a clinical syndrome. Patients experience chronic ischemic pain, ulcers, and gangrene in their lower extremities, which can lead to amputation. Among patients with severe lower extremity ischemia, 10% to 30% die within half a year, and 25% to 35% die within half a year. % required high amputation. The main causes of lower extremity ischemia are smoking, diabetes, hypertension, hyperlipidemia, hyperhomocysteinemia, etc. In recent years, with the acceleration of population aging and changes in people's diet structure, the incidence of lower limb ischemic diseases, mainly atherosclerotic obliterans, diabetic foot and thromboangiitis obliterans, has been increasing year by year in China,70 The incidence rate of people over the age of...

Claims

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Application Information

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IPC IPC(8): A61K31/722A61K31/702A61K31/7016A61K45/06A61P9/10A61P9/14
CPCA61K31/722A61K31/702A61K31/7016A61K45/06A61P9/10A61P9/14A61K2300/00
Inventor 刘洪涛朱琳郑军平杨化冰马骏孔明望姚小卫程雪
Owner HUBEI UNIV OF CHINESE MEDICINE
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