Induced amplification method of CIK2 (NK NK-T) cells

A cell and immune cell technology, applied in the field of induction and expansion of CIK2 cells, can solve the problems of low expansion rate, difference in curative effect, and low content, and achieve the effect of high expression and strong efficacy
CN112359015AActive Publication Date: 2021-02-12上海映天生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
上海映天生物科技有限公司
Publication Date
2021-02-12

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Abstract

The invention discloses an induction and amplification method of CIK2 (NK NK-T) cells. The method comprises the steps of induction and activation of immune cells CIK2 (NK NK-T) and large-capacity culture and amplification of the immune cells CIK2 (NK NK-T). The method also comprises a step of detecting the immune cell CIK2 (NK NK-T). According to the induced amplification method of the CIK2 (NK NK-T) cells, the percentage of CD16<+> / 56<+> CD3 <-> cells (NK cells) and CD16<+> / 56 <+> CD3<+> cells (NK-like T cells) can be stably increased, and the total number of cells cultured and amplified by the cells can be stably increased. The immune cell provided by the invention retains the characteristic that the NK cell has stronger efficacy of killing tumor cells and virus infected cells than the CIK1 cell; the low amplification rate of the NK cells is compensated, and the new immune cells CIK2 are also named as NK NK-T cells.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for inducing and expanding CIK2 (NK NK-T) cells. Background technique

[0002] "Cytokine-induced killer cells" (CIK) are lymphocytes isolated from human peripheral blood that are induced and activated by CD3 monoclonal antibody, γ-interferon, interleukin-1, interleukin-2 and other cytokines, and have the ability to recognize and kill tumor cells, Inhibit the virus and regulate the body's immunity and can cultivate a large number of expanded immune cells. This activated immune cell has a strong proliferation ability, mainly composed of NK-like T cells (CD56 + CD3 + ), T(CD3 + CD8 + ) dominated cell population. It has the advantages of anti-tumor activity of T lymphocytes and non-MHC-limited tumor killing of NK cells. The cells can recognize and kill a variety of tumor cells without harming normal cells in the body. The effect of infusion after surgery or radiotherapy and...

Claims

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