Induced amplification method of CIK2 (NK NK-T) cells
A cell and immune cell technology, applied in the field of induction and expansion of CIK2 cells, can solve the problems of low expansion rate, difference in curative effect, and low content, and achieve the effect of high expression and strong efficacy
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[0060]Example 1
[0061](1) Induction and activation of immune cells (CIK2):
[0062]The induction and activation of immune cells CIK2 (NK NK-T) includes the following steps:
[0063]S01: Extract 50ml of anticoagulated peripheral blood steps: use 12,500 units of heparin sodium for 1ml anticoagulation, and exclude sodium citrate anticoagulation;
[0064]S02: Coat the T75 culture flask with 7ml of "CD16 monoclonal antibody (25-35μg), Herceptin (Trastuzumab 1-2mg) " inducer for 24 hours, and then aspirate the coating solution for later use;
[0065]S03: 50ml of peripheral blood is separated to obtain 30ml of plasma (without inactivation);
[0066]S04: Lymphocytes isolated from peripheral blood 3-5×107Place the coated T75 culture flask and add 30ml of complete culture medium: AIM-V serum-free medium contains recombinant human interferon-gamma (IFN-r) for injection and recombinant human interleukin-2 (IL-2) for injection , Recombinant human interleukin-15 (IL-15), recombinant human interleukin-21 (IL-21),...
Example Embodiment
[0082]Example 2
[0083](1) Induction and activation of immune cells (CIK2):
[0084]The induction and activation of immune cells CIK2 (NK NK-T) includes the following steps:
[0085]S01: Extract 50ml of peripheral blood for anticoagulation step: use 12,500 units of heparin sodium for 1ml anticoagulation, exclude sodium citrate anticoagulation;
[0086]S02: Coat the T75 culture flask with 7ml of "CD16 monoclonal antibody 25-35μg, Herceptin (trastuzumab) 1-2mg" inducer for 24 hours, and then aspirate the coating solution for later use;
[0087]S03: 50ml peripheral blood separated and plasma (without inactivation);
[0088]S04: Lymphocytes (3-5×107Place the coated T75 culture flask and add
[0089]Complete culture medium 30ml: AIM-V serum-free medium containing recombinant human interferon-γ (IFN-r) for injection, recombinant human interleukin-2 (IL-2) for injection, recombinant human interleukin-15 (IL-15) ), recombinant human interleukin-21 (IL-21), recombinant human stem cell growth factor (SCF), adeno...
Example Embodiment
[0105]Example 3
[0106](1) Induction and activation of immune cells (CIK2):
[0107]The induction and activation of immune cells CIK2 (NK NK-T) includes the following steps:
[0108]S01: Extract 50ml of peripheral blood for anticoagulation steps: use 12,500 units of heparin sodium for anticoagulation, and exclude sodium citrate for anticoagulation;
[0109]S02: Coat the T75 culture flask with 7ml of "CD16 monoclonal antibody 25-35μg, Herceptin (trastuzumab) 1-2mg" inducer for 24 hours, and then aspirate the coating solution for later use;
[0110]S03: 50ml peripheral blood separated and plasma (without inactivation);
[0111]S04: Lymphocytes (3-5×107Place the coated T75 culture flask and add 30ml of complete culture solution: AIM-V serum-free medium contains recombinant human interferon-γ IFN-r for injection, recombinant human interleukin-2 (IL-2) for injection, recombinant Human Interleukin-15 (IL-15), Recombinant Human Interleukin-21 (IL-21), Recombinant Human Stem Cell Growth Factor (SCF), Adenos...
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