36 results about "Polyguluronic acid" patented technology

The invention discloses an alginate lyase SHA‑3 gene, the nucleotide sequence of which is shown in SEQ ID NO: 1. The invention constructs a prokaryotic expression vector of the alginate lyase SHA‑3 gene, which can be used in The expression product alginate lyase SHA‑3 was obtained in a short period of time, and it has a wide range of substrate specificity. It can use both polymannuronic acid PolyM and polyguluronic acid PolyG as substrates, and the enzyme activity reaches 12U / mg, is a bifunctional enzyme with broad application prospects, the prokaryotic expression vector and the overall expression system of the present invention are easy to operate, and are convenient for industrial production.

The invention provides a method for preparing alginate oligosaccharide monomers by using microwave radiation. The method comprises the following steps of: preparing polymannuronic acid or polyguluronic acid in algin, subjected to graded purification, into solutions or suspensions with different concentrations; performing microwave radiation in a microwave reaction system for degradation for 5 to 30 minutes; then cooling, filtering and freeze-drying the degradation product to obtain an oligosaccharide mixture; and separating and purifying the oligosaccharide mixture by using a gel column chromatography to obtain oligosaccharide monomers with different polymerization degrees. The method has the advantages that the degradation efficiency is high, the yield of oligosaccharide is high, acid or a buffer salt is not added during the degradation, few byproducts are generated, the after-treatment is simple and convenient, the product is easy to separate and purify, clean and environment-friendly effects are achieved, the cost is low, oligosaccharide mixtures with different molecular weight distributions and oligosaccharide monomers with different polymerization degrees can be prepared according to different aims and purposes, and the product is applied to various fields of foods, medicaments, chemical industry and the like.

The invention relates to gypsum tofu regulated by oligosaccharides and a preparation method thereof. The gypsum tofu comprises the following components: soybean milk, oligomeric guluronic acid and calcium sulfate; the molar concentration of oligomeric guluronic acid is 5- 75mmol / L, the molar concentration of calcium sulfate is 25-35mmol / L. The preparation method is as follows: heating soybean milk to denature protein, adding oligomeric guluronic acid and calcium sulfate in sequence after cooling down, stirring evenly, keeping warm, and then cooling down. Compared with the prior art, the oligomeric guluronic acid added in the present invention is an oligosaccharide, which can specifically bind calcium ions, regulate the texture properties of tofu, and as a water-soluble dietary fiber can promote excessive Calcium ions are excreted from the body, reducing the health risks caused by long-term intake of gypsum food; oligomeric guluronic acid also has excellent water holding capacity and biological activity, which can effectively improve the nutritional value of tofu, and the prepared gypsum tofu is better than traditional gypsum The tofu has high water content, low chewiness, simple preparation method and broad market prospect.