The invention discloses a thermophilic esterase derived from an Aquifex aeolicus strain and an expression thereof and belongs to the technical field of biomedical engineering. A novel thermophilic esterase gene is found, three expression systems are established, and efficient expression and enzymatic property research are achieved. A eukaryotic expression system is established, wherein a carrier pPIC9K is preferred for expression vector establishment, a pichia pastoris host is converted, GS115 is preferred, and efficient expression is achieved. A prokaryotic escherichia coli expression system is established, wherein a carrier MBP3 is preferred for expression vector establishment, an escherichia coli host is converted, BL21 and Origami2 are preferred, and efficient expression is achieved. A prokaryotic bacillus megatherium expression system is established, wherein a carrier pHIS1525 is preferred for expression vector establishment, a bacillus megatherium host is converted, YYBm1 is preferred, and efficient expression is achieved. The recombinant enzyme has the advantages of esterase activity, thermophily, thermal stability and the like and has great potential in industrial applications under the high temperature condition.