Alginate lyase SHA-2 gene and expression vector thereof

Abstract

The invention discloses an alginate lyase SHA-2 gene. A nucleotide sequence is as shown in SEQ ID NO:1. A prokaryotic expression vector of the alginate lyase SHA-2 gene is constructed; an expression product alginate lyase SHA-2 can be obtained by the prokaryotic expression vector within a relatively short period of time, and has wide substrate specificity; poly-mannuronate (PolyM) and poly-guluronate (PolyG) can also be utilized as substrates; the enzyme activity can reach 5.2U / mg; the alginate lyase SHA-2 gene is a bifunctional enzyme with a wide application prospect; the prokaryotic expression vector and an overall expression system are easy to operate; and industrialized production is facilitated.

Description

technical field

[0001] The invention belongs to the field of microbial genetic engineering, in particular to an alginate lyase SHA-2 gene and its prokaryotic expression vector pGEX-4T-1- SHA-2 , the vector highly expresses the alginate lyase protein SHA-2. Background technique

[0002] In recent years, the development and utilization of marine resources has gradually become a research hotspot. Because of its unique physical and chemical properties, alginic acid has broad application prospects in the fields of food, medicine and chemical industry. Alginate oligosaccharides have become the focus of new drug development because of their various physiological activities. At the same time, as one of the most abundant marine biomass, alginic acid has the following advantages: (1) high photosynthetic efficiency, fast growth, high yield, and abundant resources; (2) growth does not occupy arable land; (3) hardly contains wood The content of cellulose is low, the pretreatment is s...

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