Neutral low-temperature xylanase CaXyn10A, and gene and application thereof

A technology of xylanase and low temperature, applied in the field of genetic engineering
CN105950591AActive Publication Date: 2016-09-21INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Publication Date
2016-09-21

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Abstract

The invention relates to the field of genetic engineering, in particular to a neutral low-temperature xylanase CaXyn10A, and a gene and application thereof. The amino acid sequence of the neutral low-temperature xylanase CaXyn10A is shown as SEQ ID NO. 1. The xylanase is characterized in that the optimal pH is 6.0-6.5, the optimal temperature is 40 DEG C, over 20% of enzyme activity is kept at the temperature of 0 DEG C, and the specific activity is 453U / mg; the xylanase has activities of xylanase, glucanase and cellulose; industrialized fermentation production is facilitated. The neutral low-temperature xylanase CaXyn10A serving as a novel broad-spectrum preparation can be widely applied to aquatic feed, food, papermaking, energy industry and the like.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a neutral low-temperature xylanase CaXyn10A and its gene and application. Background technique

[0002] Xylan (xylan) is the main component of plant biomass, its content in nature is second only to cellulose (Lyndet al. Microbiology and Molecular Biology Review 2002,66:506–577), and has potential use in bioenergy value. Existing biotechnology can efficiently degrade the cellulose component in plants and convert it into fermentable glucose, which is produced by yeast to produce bioethanol. High-component xylan not only limits the combination reaction of cellulase and cellulose, inhibits the catalytic reaction, but also discharges into nature as a by-product, which can cause nutrient enrichment and damage the ecosystem (Polizeli et al. Applied Microbiology and Biotechnology. 2005, 67:577-591). Therefore, in the process of biomass bioconversion, xylanase ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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