Method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots

Abstract

The invention relates to a method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots. The method utilizes the panax quinquefolius fibrous roots as raw materials for extraction and comprises sequential steps as follows: grinding, alkaline leaching, enzymolysis with compound enzyme, extraction with an ultrasonic method, concentration, ethanol precipitation and vacuum drying. According to the method, a traditional water or ethanol extraction method for the panax quinquefolius polysaccharides is broken through, an alkaline solution with certain concentration is utilized to promote the physicochemical reaction in panax quinquefolius polysaccharide grains or among the panax quinquefolius polysaccharide grains for alkaline leaching extraction, the extraction is assisted with composite enzymatic hydrolysis and ultrasonic wave technologies through combination of a wall-breaking effect of cellulase on plant cells and a specific degradation effect of protease, the process is simple and easy to operate, the extraction rate and the yield of the panax quinquefolius polysaccharides are increased remarkably, and theoretical reference bases are provided for industrial production of the extraction of the panax quinquefolius polysaccharides.

Description

technical field [0001] The invention relates to an extraction method, in particular to a method for extracting American ginseng polysaccharides from American ginseng fibrous roots. Background technique [0002] American ginseng (Panax quinquefolius L.) is the dried root of Araliaceae Panax, a perennial herb, also known as American ginseng, American ginseng, American ginseng, Cantonese ginseng, etc., native to Canada, the United States, France and other places. Since the 1980s, large-scale introductions have been made in my country. At present, the plantings are mainly concentrated in the three northeastern provinces, Shandong, and Beijing. At present, my country has become the third largest producer of American ginseng in the world, and is also the largest consumer of American ginseng in the world. American ginseng has similar functions to ginseng, but it is different from ginseng because of its cool nature. American ginseng polysaccharide (PPQ) is the most abundant substan...

AI Technical Summary

Problems solved by technology

[0003] At present, the research on the extraction process of American ginseng polysaccharides is mainly based on traditional extraction methods such as hot water extraction, water extraction and alcohol precipitation. After investigation, the existing Chinese patent No. CN201110157090.1 "Preparation and Production Method of American Ginseng Polysaccharide Peptides" , including the following steps: using American ginseng stems, leaves, and fibrous roots as raw materials, extracting with water, the ratio of solid to liquid is 1:10, at 60°C, the extraction time is 2h, twice, filtering and combining the filtrates, vacuum concentrating and filtering to a concentration of 10:1, Precipitate with 75% alcohol, centrifuge the supernatant to recover alcohol and extract saponin, then elute the precipitate with 95% edible alcohol, and recover alcohol; dissolve the precipitate with water at 1:10, add 1% flavor protease, at 55°C, PH 5.6, hydrolyzed for 4 hours, inactivated enzymes, filtered and concentrated in vacuum, and spray-dried to make off-white American ginseng polysaccharide peptides. This method adopts the traditional water extraction and alcohol precipitation extraction method and single enzymatic hydrolysis auxiliary process, and the extraction rate of active ingredients is low, Disadvantages such as low yield, cumbersome operation, and long time consumption