Method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots

A technology of American ginseng polysaccharides and American ginseng, which is applied in the field of extraction, can solve the problems of low extraction rate of active ingredients, cumbersome operation, and low yield, and achieve the effect of improving extraction rate and yield, simple process, and improving extraction rate

Active Publication Date: 2017-02-15
宁波中药制药股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on the extraction process of American ginseng polysaccharides is mainly based on traditional extraction methods such as hot water extraction, water extraction and alcohol precipitation. After investigation, the existing Chinese patent No. CN201110157090.1 "Preparation and Production Method of American Ginseng Polysaccharide Peptides" , including the following steps: using American ginseng stems, leaves, and fibrous roots as raw materials, extracting with water, the ratio of solid to liquid is 1:10, at 60°C, the extraction time is 2h, twice, filtering and combining the filtrates, vacuum concentrating and filtering to a concentration of 10:1, Precipi

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] After pulverizing 100g of dried American ginseng fibrous roots with a pulverizer, pass through a 40-mesh sieve for later use. According to the material-liquid ratio of 1:25 (g:ml), add 3% sodium hydroxide at 90°C to soak for 3 hours, filter the filter residue, and evaporate the filter residue; The mass fraction of the enzyme bottom is 1% compound enzyme (cellulase:papain=1:1), and it is enzymolyzed on a constant temperature oscillator at 50°C for 1 hour; the filter residue of enzymolysis is 1:28 (g:ml) ) into distilled water, 60W ultrasonic extraction three times, each time for 20min, and centrifuge to collect the filtrate; the obtained filtrate material is concentrated under reduced pressure, the temperature of the reduced pressure concentration is 60°C, the pressure is 0.07~0.09MPa, and the concentrated relative density is 1.25; Add absolute ethanol after liquid cooling, the final volume fraction of ethanol reaches 80%, leave standstill for 12 hours, collect precipita...

Embodiment 2

[0033] After pulverizing 100g of dried American ginseng fibrous roots with a pulverizer, pass through a 40-mesh sieve for later use. According to the material-liquid ratio of 1:20 (g:ml), add 2% sodium hydroxide at 90°C to soak for 2 hours, filter the filter residue, and evaporate the filter residue; the obtained filter residue is added according to the material-liquid ratio of 1:8 (g:ml). The mass fraction of the enzyme base is 1% compound enzyme (pectinase:papain=1:1), and it is enzymolyzed on a constant temperature oscillator at 45°C for 1 hour; the filter residue of enzymolysis is 1:25 (g:ml) ) into distilled water, 45W ultrasonic extraction three times, 15min each time, and centrifuge to collect the filtrate. The obtained filtrate is concentrated under reduced pressure, the temperature of the reduced pressure concentration is 50°C, the pressure is 0.07-0.09MPa, and the concentrated relative density is 1.30; absolute ethanol is added after the concentrated solution is cool...

Embodiment 3

[0035] After pulverizing 100g of dried American ginseng fibrous roots with a pulverizer, pass through a 40-mesh sieve for later use; add 4% sodium hydroxide at 90°C to soak for 3 hours according to a material-liquid ratio of 1:23 (g:ml), and filter to obtain a filter residue, which is evaporated. dry; the obtained filter residue is 1:12 (g:ml) according to the ratio of material to liquid, and the mass fraction of enzyme base is 1% compound enzyme (cellulase: bromelain=1:1), and the enzyme is fermented on a constant temperature oscillator at 50°C Solution for 1 hour. Add distilled water to the enzymolyzed filter residue according to the ratio of material to liquid 1:30 (g:ml), 50W ultrasonic extraction three times, each time for 25min, and centrifuge to collect the filtrate; It is 0.07~0.09MPa, and the concentrated relative density is 1.25; add absolute ethanol after cooling the concentrate, the final volume fraction of ethanol reaches 75%, let it stand for 12 hours, and collec...

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Abstract

The invention relates to a method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots. The method utilizes the panax quinquefolius fibrous roots as raw materials for extraction and comprises sequential steps as follows: grinding, alkaline leaching, enzymolysis with compound enzyme, extraction with an ultrasonic method, concentration, ethanol precipitation and vacuum drying. According to the method, a traditional water or ethanol extraction method for the panax quinquefolius polysaccharides is broken through, an alkaline solution with certain concentration is utilized to promote the physicochemical reaction in panax quinquefolius polysaccharide grains or among the panax quinquefolius polysaccharide grains for alkaline leaching extraction, the extraction is assisted with composite enzymatic hydrolysis and ultrasonic wave technologies through combination of a wall-breaking effect of cellulase on plant cells and a specific degradation effect of protease, the process is simple and easy to operate, the extraction rate and the yield of the panax quinquefolius polysaccharides are increased remarkably, and theoretical reference bases are provided for industrial production of the extraction of the panax quinquefolius polysaccharides.

Description

technical field [0001] The invention relates to an extraction method, in particular to a method for extracting American ginseng polysaccharides from American ginseng fibrous roots. Background technique [0002] American ginseng (Panax quinquefolius L.) is the dried root of Araliaceae Panax, a perennial herb, also known as American ginseng, American ginseng, American ginseng, Cantonese ginseng, etc., native to Canada, the United States, France and other places. Since the 1980s, large-scale introductions have been made in my country. At present, the plantings are mainly concentrated in the three northeastern provinces, Shandong, and Beijing. At present, my country has become the third largest producer of American ginseng in the world, and is also the largest consumer of American ginseng in the world. American ginseng has similar functions to ginseng, but it is different from ginseng because of its cool nature. American ginseng polysaccharide (PPQ) is the most abundant substan...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 欧文静王萌盛雷安亚楠
Owner 宁波中药制药股份有限公司
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