Alginate lyase SHA-3 gene and prokaryotic expression vector thereof

A technology of prokaryotic expression of alginate lyase, which is applied in the field of microbial genetic engineering, can solve the problems of difficulty in meeting application requirements, high cost, and low enzyme production of wild-type alginate-decomposing bacteria, and achieve broad substrate specificity, Easy to operate and convenient for industrial production
CN104878030AActive Publication Date: 2015-09-02KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2015-09-02

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Abstract

The invention discloses an alginate lyase SHA-3 gene with a nucleotide sequence as shown in SEQ ID NO:1. The invention establishes a prokaryotic expression vector of the alginate lyase SHA-3 gene. The vector can obtain an expression product, namely alginate lyase SHA-3 within relatively-short time, has wide substrate specificity, can take polymannuronic acid PolyM or polyguluronic acid PolyG as a substrate, and has the enzyme activity of 12U / mg so as to be a difunctional enzyme with a wide application prospect. The prokaryotic expression vector and a whole expression system are easily operated and are conveniently used for realizing industrial production.
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Description

Technical field

[0001] The invention belongs to the field of microbial genetic engineering, which involves a pGEx-4T-3 gene and its primary expression carrier PGEx-4T-3 SHA-3 , This carrier expresses the alginic acid pest enzymes SHA-3. Background technique

[0002] In recent years, the development and utilization of marine resources has gradually become a hotspot of research. Because of its unique physical and chemical properties, sea alginic acid has wide application prospects in food, medicine and chemical industry.For alginate oligosaccharides, because of a variety of physiological activity, it has become a focus point for developing new drugs.At the same time, as one of the richest marine biomass, sea alginic acid has the following advantages: (1) High photosynthetic efficiency, fast growth, high yield, and rich resources; (2) growth does not occupy cultivated land;Vegetarian, the content of cellulose is very small, the pre -processing is simple, which is convenient for the ...

Claims

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