Thermophilic esterase derived from Aquifex aeolicus strain and expression thereof

Abstract

The invention discloses a thermophilic esterase derived from an Aquifex aeolicus strain and an expression thereof and belongs to the technical field of biomedical engineering. A novel thermophilic esterase gene is found, three expression systems are established, and efficient expression and enzymatic property research are achieved. A eukaryotic expression system is established, wherein a carrier pPIC9K is preferred for expression vector establishment, a pichia pastoris host is converted, GS115 is preferred, and efficient expression is achieved. A prokaryotic escherichia coli expression system is established, wherein a carrier MBP3 is preferred for expression vector establishment, an escherichia coli host is converted, BL21 and Origami2 are preferred, and efficient expression is achieved. A prokaryotic bacillus megatherium expression system is established, wherein a carrier pHIS1525 is preferred for expression vector establishment, a bacillus megatherium host is converted, YYBm1 is preferred, and efficient expression is achieved. The recombinant enzyme has the advantages of esterase activity, thermophily, thermal stability and the like and has great potential in industrial applications under the high temperature condition.

Description

technical field [0001] The invention relates to a thermophilic esterase derived from Aquifex aeolicus bacterial strain and its expression, belonging to the technical field of bioengineering. Background technique [0002] Extremophiles are the general term for microorganisms suitable for living in extreme environments, including thermophilic, psychrophilic, acidophilic, alkaliphilic, barophilic, halophilic, metal-loving, radiation-resistant, desiccation-resistant and extreme anaerobic and other types . Thermophilic bacteria (high-temperature bacteria, also known as thermophilic microorganisms) are a class of microorganisms that live in high-temperature environments above 50°C. Common sources of thermophilic microorganisms include hot springs, craters, marine sediments, waste water, geothermal soils, etc. In the past 30 years, after the discovery of bacteria that can live at and above the boiling point of water, the research on thermophilic microorganisms has been promoted. ...

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Problems solved by technology

However, most thermophilic bacteria grow slowly and the culture conditions are strict, so it is

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