41 results about "Biological stain" patented technology

A firearm ammunition device includes a cartridge with a projectile that is loaded with a tracer agent. When the projectile is fired and strikes a game animal, the impact causes the release and dispersal of the tracer agent from the projectile. The dispersed tracer agent identifies an enhanced-visibility trail of the fleeing animal. In example embodiments, the cartridge is provided by a centerfire cartridge, a shotgun cartridge, and a muzzleloading cartridge. The tracer agent preferably comprises one or more compounds that produce visible light to the naked eye and / or under a black light source. Example tracer agents include biological stains that produce visible light upon contact with blood, luminol or another chemiluminescent compound that releases light by a chemical reaction such as may occur when contacting blood, a basic salt such as sodium carbonate or another effervescence-inducing agent, a phosphorescent compound, or a fluorescent compound.

A firearm ammunition device includes a cartridge with a projectile that is loaded with a tracer agent. When the projectile is fired and strikes a game animal, the impact causes the release and dispersal of the tracer agent from the projectile. The dispersed tracer agent identifies an enhanced-visibility trail of the fleeing animal. In example embodiments, the cartridge is provided by a centerfire cartridge, a shotgun cartridge, and a muzzleloading cartridge. The tracer agent preferably comprises one or more compounds that produce visible light to the naked eye and / or under a black light source. Example tracer agents include biological stains that produce visible light upon contact with blood, luminol or another chemiluminescent compound that releases light by a chemical reaction such as may occur when contacting blood, a basic salt such as sodium carbonate or another effervescence-inducing agent, a phosphorescent compound, or a fluorescent compound.

The invention provides type I unsymmetrical cyanine fluorescent dye, wherein X, n, R1, R2, R3, R4, R5 and Y<-> are defined as in the instruction book. The invention also provides the conjugate and the preparation method of the fluorescent dye, the combination containing the fluorescent dye and the biological dyeing method by using the fluorescent dye or the combination.

The invention discloses a preparation method of a metal-graphene composite coating. The preparation method comprises steps as follows: firstly, metal-graphene composite particles are prepared; a matrix is subjected to surface treatment, and the composite coating is prepared on the surface of the matrix with a thermal spraying technology. The preparation method of the metal-graphene composite coating is simple to operate, low in cost, high in efficiency and suitable for industrial production. The prepared metal-graphene composite coating has a compact structure, has good bonding property with the matrix, has the thickness of 10 mu m-10 mm and has corrosion resistance, friction resistance, high mechanical strength, anti-bacterial property and biological stain and damage prevention property.The metal-graphene composite coating has good application prospects and economic benefits in the fields of friction resistance, corrosion resistance and biological stain and damage prevention.

The invention provides a biological stain-resistant coating with long-acting super-hydrophilic performance and a preparation method thereof. The preparation method comprises the following steps that pre-treatment is conducted on the surface of a base material; the treated base material is placed in slightly acidic buffer liquid, and then a polyphenol compound, a polyamine-based compound and an oxidizing agent are added for reaction; a reaction product is immersed in deionized water for ultrasonic cleaning, and then under a nitrogen condition, drying is conducted to obtain the biological stain-resistant coating with the long-acting super-hydrophilic performance. The method is simple to operate and mild in reaction condition, a prepared coating material has the polyphenol compound and a large amount of functional groups of carboxyl, phenolic hydroxyl, quinonyl, amido and the like, and the surface of the coating shows the long-acting super-hydrophilic performance. The adhesiveness betweenthe modified coating prepared by adopting the method and the base material is excellent, and the super-hydrophilic performance of the surface of the coating makes the material have an excellent biological stain-resistant function, so that the biological stain-resistant coating with the long-acting super-hydrophilic performance can be used for preparing materials of implanted devices, medical diagnosis instruments, medical sensors and the like.

A bowhunting tracking-assist device on an arrow's shaft includes a tracer agent and an agent dispenser. The dispenser releases the agent when the arrow strikes a game animal, and the released agent identifies an enhanced-visibility trail of the fleeing animal. In an example embodiment, the dispenser has wells for holding the agent and a cover that displaces to release the agent when the arrow drives into the animal. The tracer agent preferably comprises one or more compounds that produce visible light to the naked eye and / or under a black light source. Example tracer agents include biological stains that produce visible light upon contact with blood, luminol or another chemiluminescent compound that releases light by a chemical reaction such as may occur when contacting blood, a basic salt such as sodium carbonate or another effervescence-inducing agent, a phosphorescent compound, or a fluorescent compound.

A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.

A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.

The invention discloses a waterborne polyurethane leather coating agent with a biological stain and damage prevention function. According to a preparation method, firstly, through sulfydryl-double-bond clicking reaction, isoborneol monomers containing di-hydroxyls are synthesized; then, through the addition polymerization reaction, the isoborneol monomers are introduced into the polyurethane main chains; further, through the chain expansion reaction and the neutralization reaction, the waterborne polyurethane leather coating agent with the biological stain and damage prevention function is obtained. The isoborneol monomers have good biological stain and damage prevention performance; when the molecular weight is greater, the biological stain and damage prevention performance is better. Through the generation of addition polymerization reaction by diisocyanate and di-hydroxyls in the structure, the waterborne polyurethane leather coating agent can be introduced onto the polyurethane main chain in the molecule level. Good water-resistant performance and film forming performance of the polyurethane leather coating agent are maintained; meanwhile, the durable biological stain and damage prevention performance is realized.

The invention relates to an improved periodic acid Schiff (PAS) rapid staining kit. The kit comprises: a staining liquid, shedding-proof films, and cover slips. The staining liquid is a mixture of active fuchsine liquid and active fast green liquid, which is diluted with 95% of ethanol and 0.5% of sodium binitrite solution. The kit is advantaged in that: fuchsine and fast green are firstly prepared into a rapid biological rapid staining liquid; the staining process is fast, wherein the staining process takes 10 seconds; the detection process is simple, wherein a result can be observed directly, and washing is not required; the application scope is wide, wherein the kit can be used for staining various clinic specimens such as scurf, mucous and vaginal secretion. Periodic acid is attached on object slides, such that packing materials of the product is saved, and the operation process is simplified. The kit is especially suitable for a large clinic specimen amount. Because periodic acid, which is a flammable and combustible product, is attached to the object slides, periodic acid turns from liquid into a dried status. Therefore, danger of periodic acid is greatly reduced, and a standardized operation is easy to achieve. Poor repeatability due to personal operation errors can be avoided.

The invention provides a conjugate of a polymer and a biological stain and a preparation method and application of the conjugate. The conjugate has the formula G-X-B, wherein the polymer G is selected from the group consisting of glucans, fructosans, polymannuronic acid, polyguluronic acid, heteropolysaccharides, mono- or hetero-polysaccharides, mono- or hetero-polyuronic acid polysaccharides, mono- or hetero-polyuronic acid sulfate esters, hydrophilic alcohol high polymers, polylysine, polyglutamic acid and polyaspartic acid; the linking group X is a C1-C10 organic group; and the biological stain or a derivative B thereof may be toluidine blue, methylene blue, Evans blue, neutral red, Janus green or bright cresol purple. The conjugate can stain bilateral neck and submaxillary or submental lymphatic vessels and lymph nodes in New Zealand white rabbits by indirect lymph staining method, without diffusion in tongue body. No obvious toxic and adverse effects appear in all experimental animals in each group, and no obvious pathological changes appear in histological examination of internal organs.

The invention relates to a liquid-dropping type automatic biological staining instrument; the top end of a machine frame is provided with a loading port; a vertical track extends downward to the interior of the machine frame from the loading port; a slide box is arranged at the loading port, can rise and fall up and down along the vertical track, and is internally provided with slides for bacteria staining; a transverse track is transversely arranged inside the machine frame; the rear end of a manipulator is in sliding connection on the transverse track, and the front end of the manipulator is used for clamping the slides in the slide box; a liquid-dropping type staining and cleaning device is arranged at the top end of the machine frame; a drying device is arranged inside the machine frame; a waste liquid cup is arranged at the bottom end of the machine frame and is used for containing a waste liquid of a staining liquid and a cleaning waste liquid; a control module is arranged on the side face of the machine frame, and is electrically connected with the manipulator, the liquid-dropping type staining and cleaning device and the drying device. Compared with the prior art, the liquid-dropping type automatic biological staining instrument has the advantages of simple operation, reliable method and stable performance, and can meet various staining ways.

The invention provides asymmetrical cyanine fluorescent dye expressed by formula I, wherein X, n, R1, R2, R3, R4 and Y are defined as a specification. The dye can be used as excellent nucleic acid staining agent, and the spectrum of the dye is in a near infrared region of 600 to 900 nanometers and has no interference of background fluorescence. Simultaneously, the dye can utilize a small red semiconductor laser as a light source (633 nanometers). The invention also provides a composition comprising the fluorescent dye and a method for performing biological stain by using the fluorescent dye or the composition thereof.

The invention discloses a polymeric compound coating and a preparation method and an application thereof. The polymeric compound coating disclosed by the invention has excellent corrosion resistant performance and biological stain resistance, and has a wide-spectral, efficient and lasting antibacterial effect. The preparation method disclosed by the invention is simple in process, few in environmental pollution and high in efficiency, spraying is not limited by dimension and shape of a workpiece, and the coating is low in cost and suitable for being prepared in a large area. The polymeric compound coating disclosed by the invention has good application prospect and economical benefit in the field of corrosion resistance and marine stain prevention.

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays, systems, methods and kits for the age determination of an individual from bloodstains or samples of unknown origin. The methodology is based on gene expression profiling analysis in which novel human newborn fetal specific genes are identified by detecting the presence of appropriate messenger RNA species.

The invention discloses 2, 5-di (rhodamine B-9'-(2''-benzoyl) radical)-1, 3, 4-diazole fluorescent stain compound I and a preparation method and application thereof. The compound I has excellent dissolubility in water, emits strong fluorescent light and can be applied to being biological stain, namely cell stain, and water solution of the compound I cannot suddenly extinct in fluorescence spectrum in a range of the pH (potential of hydrogen) value from 2 to 10. The compound I can be prepared from rhodamine B and phosphorus oxychloride directly or can be prepared through intermediate compound IIN and N'-double-rhodamine B hydrazine. The invention further discloses the application of the structural formula I serving as biological stain, namely cell stain.

The invention discloses preparation methods of prodigiosin and prodigiosin-containing biological stain as well as an application of the prodigiosin-containing biological stain in fabric. The preparation method of the prodigiosin comprises the following steps: (1), red pigment stains with the preservation number of CGMCC (China General Microbiological Culture Collection Center) 4074 are cultured in a microbial culture solution, and prodigiosin-containing fermentation broth is obtained; (2), the prodigiosin-containing fermentation broth is subjected to centrifugation and supernatant removal to obtain a precipitate, an organic solvent is added to the precipitate, the mixture is subjected to centrifugation, the organic solvent in supernatant is evaporated, and prodigiosin solids are obtained. The special microbial culture solution can improve the output of the prodigiosin, and industrial waste protein products can be adopted as raw materials to replace imported peptone used for fermentation production of the prodigiosin; according to a preferable technical scheme, the output of the prodigiosin can be further increased with adoption of mixed culture, and the antibacterial property is improved.

The invention discloses a water quality sensor. The sensor comprises a shell and an optical window arranged in the shell. The water quality sensor also comprises an ultraviolet light emitting source and a medium part which is used as the ultraviolet light transmitting medium. The ultraviolet light emitting source and the medium part are configured so as to make at least part of the ultraviolet lights emitted by the ultraviolet light emitting source are completely internally reflected in the optical window. The water quality sensor is provide with an ultraviolet light emitting source, which can emit ultraviolet lights to prevent and eliminate biological stains that may attach on the optical window. Besides, the ultraviolet lights are all confined in the optical window because of the light complete internal reflection, thus the total radiation strength is enhanced, so the water quality sensor can prevent biological pollution for a longer time, and the on-site continuous operation time of the water quality sensor is increased. The water quality sensor has the advantages of simple structure and low cost.

The invention belongs to the field of biomedical materials, and discloses moxifloxacin drug derivatives as well as a preparation method and application thereof. The moxifloxacin drug derivative has astructure shown as a formula I, wherein R is independently an organic cation of triphenylphosphine salt, pyridinium salt and quaternary ammonium salt; x is a monovalent anion; and n is any integer from 1 to 17. The invention also discloses a preparation method of the moxifloxacin drug derivative. The moxifloxacin drug derivative disclosed by the invention has an aggregation-induced emission property, can realize rapid staining recognition on microorganisms, and shows the characteristics of specificity, high efficiency and sensitivity. The moxifloxacin drug derivative disclosed by the inventionhas an excellent antibacterial effect. The moxifloxacin drug derivative disclosed by the invention is used for preparing reagents for specific dyeing of mitochondria and dyeing of microorganisms withnegative charges on the surfaces, and is also used for preparing reagents and antibacterial agents for distinguishing dead and living states of microorganisms.

The invention discloses a stable biological stain and a preparation method of same. The biological stain is composed of, by mass, 1-15% of trinitrophenol, 2-30% of sodium persulfate, 3-25% of acrylate, 1-15% of medical-grade acetic acid, 0.5-15% of 5,6,7,8-tetrahydrofolic acid, 0.1-5% of horseradish peroxidase, 1-10% of formalin, 1-30% of ethanol, and the balanced being deionized water. The preparation method includes the steps of: 1) fully mixing the deionized water with the trinitrophenol, the sodium persulfate and the medical-grade acetic acid, allowing the mixture to stand for precipitating, and collecting a supernatant; 2) mixing the supernatant with the acrylate, the 5,6,7,8-tetrahydrofolic acid, the horseradish peroxidase and the ethanol, and heating the mixture at 30-80 DEG C in water bath for 5-50 min, during the water bath heating process, the formalin is added; and 3) uniformly stirring the mixture to obtain the biological stain. The biological stain is low in usage amount,has good staining effect, can form clear colors, is easy to elute in later processes, is free of changing hardness of a sample and is high in sample completeness. The biological stain allows the sample to be quickly, high-effectively and accurately displayed in anatomy of biomedicine.

A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.

The invention discloses a method for measuring the thickness of trunk water diversion sapwood. The method for measuring the thickness of the trunk water diversion sapwood comprises the following steps: using a growth cone to drill a small hole at a position 10cm below the measuring position of trunk, removing the wooden core inside, and sealing the mouth with a rubber plug; putting a biological staining reagent in a sealed plastic bag, and using a medical hose and plastic needle to introduce the staining reagent into the small hole through the rubber stopper to ensure that the staining agent fills the entire small hole; and using the growth cone to drill the wood core at a position 10 cm above the small hole vertically after 2 to 3 hours of dyeing, so that the part stained with the staining reagent is the active water diversion sapwood, and the length of the part is measured to be the width of the water diversion sapwood. According to the invention, the tree core is drilled and stainedin the wild; the operation is simple and easy; the damage to the tree is small; the accuracy is high; and the accuracy of sapwood thickness measurement is greatly improved.

The invention relates to a dye and a preparation method thereof. The fluorescent dye is a series of novel dye which takes PnO2-PODIPY / PnO2-azaPODIPY developed from dipyrrin / aza-dipyrromethene complexing PnO2 (Pn-P, As, Sb, Bi) as a parent nucleus, and the general formula of the fluorescent dye is as shown in I / II. The fluorescent dye disclosed by the invention has the advantages of spectral property and the like of the classic traditional dye BODIPY / azaBODIPY. Although a central atom Pn is a sexadentate, the fluorescent dye is stable without being decomposed in air and a water solution. The compound has the outstanding characteristics of excellent water solubility and certain cell membrane permeability and can be used for biological dyeing. The novel dye disclosed by the invention has a novel spectral characteristic and can be applied to the aspect of fluorescence imaging.

The invention discloses a fibrilia biological dyeing technology based on laccase catalytic polymerization reaction. The fibrilia biological dyeing technology comprises the following steps: A, carryingout singeing treatment on fibrilia; B, immersing and treating singed fibrilia into an alkaline solution, and removing surface impurities and residual slurry; C, putting and repeatedly washing the fibrilia after alkaline treatment in clear water, and putting and drying the fibrilia in a low-temperature drying oven; D, immersing the fibrilia after the drying treatment in a buffer solution containing a phenols monomer and laccase, and carrying out biological dyeing; E, carrying out color fixing and steaming treatment on the fibrilia after the biological dyeing; F, finally carrying out soap boiling and water washing on the fibrilia. The fibrilia biological dyeing technology adopted by the invention is simple in operation and low in cost, is environment-friendly and pollution-free and is capable of improving dyeing and color fixing effects of the fibrilia.

The invention provides an asymmetric cyanine fluorescent dye expressed in the formula I (please see the formula in the specification). X, n, R1, R2, R3, R4 and Y are defined in the specification. The dye can be used as an excellent nucleic acid dyeing agent, the spectrum of the dye is in a near infrared region of 600 nm to 900 nm, and interference of background fluorescence does not exist. Meanwhile, according to the dye, a small red semiconductor laser can be used as a light source (633 nm). The invention further provides a composition containing the fluorescent dye and a method for using the fluorescent dye or the composition of the fluorescent dye in biological dyeing.

The invention discloses an automatic cleaning device for ship biofouling, which comprises a mounting frame, a cover plate, a moving mechanism, a lifting mechanism, a base plate and a high-pressure water gun. A cover plate is fixedly installed in the mounting frame, and the cover plate A moving mechanism is installed between the surface and the mounting frame, and a lifting mechanism is fixedly installed on the upper surface of the cover plate. The end of the lifting mechanism away from the cover plate is fixedly connected with a base plate, and the end of the base plate away from the lifting mechanism is installed with a high-pressure water gun. The operation of the automatic removal device for ship biofouling provided by the present invention is realized through mechanical transmission, which can greatly reduce the working intensity of the staff, and does not require too many personnel to participate in the automatic operation of the biofouling at the bottom of the ship, and the barnacle fragments produced are not It will cause the danger of scratches to the staff. The use of the eccentric wheel structure makes the spray head of the high-pressure water gun can be controlled to rotate reciprocally within a certain range, and the cleaning effect is more comprehensive.

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays, systems, methods and kits for the age determination of an individual from bloodstains or samples of unknown origin. The methodology is based on gene expression profiling analysis in which novel human newborn fetal specific genes are identified by detecting the presence of appropriate messenger RNA species.

The invention discloses a waterborne polyurethane leather coating agent with a biological stain and damage prevention function. According to a preparation method, firstly, through sulfydryl-double-bond clicking reaction, isoborneol monomers containing di-hydroxyls are synthesized; then, through the addition polymerization reaction, the isoborneol monomers are introduced into the polyurethane main chains; further, through the chain expansion reaction and the neutralization reaction, the waterborne polyurethane leather coating agent with the biological stain and damage prevention function is obtained. The isoborneol monomers have good biological stain and damage prevention performance; when the molecular weight is greater, the biological stain and damage prevention performance is better. Through the generation of addition polymerization reaction by diisocyanate and di-hydroxyls in the structure, the waterborne polyurethane leather coating agent can be introduced onto the polyurethane main chain in the molecule level. Good water-resistant performance and film forming performance of the polyurethane leather coating agent are maintained; meanwhile, the durable biological stain and damage prevention performance is realized.

The invention discloses a preparation method and a microscopic examination method of chitosan-embedded calcium alginate microcapsules. The preparation method comprises the following steps: mixing active microorganisms and a biological coloring agent according to a preset ratio to obtain a dyed active microorganism liquid; mixing soluble alginate, soluble carbonate, soluble calcium salt, water and the dyed lactic acid bacteria liquid according to a preset ratio, and performing primary embedding to obtain dyed calcium alginate microcapsule particles; adding the obtained dyed calcium alginate microcapsule particles into a chitosan solution to obtain a chitosan-embedded calcium alginate microcapsule subjected to secondary embedding; compared with the current situation that the chitosan-embedded calcium alginate microcapsule prepared by the existing method has higher requirements on the sample form and equipment, the chitosan-embedded calcium alginate microcapsule prepared by the preparation method has a certain color development effect, can be clearly observed under an optical microscope, has low requirements on the microscopic examination sample form, is low in microscopic examination equipment cost, and is suitable for industrial production. The microscopic examination process is fast and convenient, and the microscopic examination result is accurate.

The invention relates to a potential type anti-biofouling sensor, in particular to a polymer film potential type anti-biofouling sensor based on a graphene oxide antifouling layer and an application thereof. The polymer film potential type anti-biofouling sensor is composed of a conventional disc electrode, a polymer sensitive film and a graphene oxide layer; one end of the conventional disc electrode is adhered to the polymer sensitive film and the graphene oxide layer in sequence. The polymer membrane potential type anti-biofouling sensor based on the graphene oxide antifouling layer of the present invention can improve the hydrophilicity and killing properties of the polymer sensitive membrane by means of the graphene oxide material, so as to prevent the formation of biological adhesion and biofilm Function, realize the anti-biofouling performance of the polymer membrane potential sensor, so as to solve the biofouling problem that the polymer membrane potential sensor often faces when it is applied in complex water bodies. The polymer membrane potential type anti-biofouling sensor based on the graphene oxide antifouling layer of the present invention has the advantages of simple preparation and long duration of antifouling performance.