41 results about "Biological stain" patented technology

The invention provides type I unsymmetrical cyanine fluorescent dye, wherein X, n, R1, R2, R3, R4, R5 and Y<-> are defined as in the instruction book. The invention also provides the conjugate and the preparation method of the fluorescent dye, the combination containing the fluorescent dye and the biological dyeing method by using the fluorescent dye or the combination.

The invention provides a biological stain-resistant coating with long-acting super-hydrophilic performance and a preparation method thereof. The preparation method comprises the following steps that pre-treatment is conducted on the surface of a base material; the treated base material is placed in slightly acidic buffer liquid, and then a polyphenol compound, a polyamine-based compound and an oxidizing agent are added for reaction; a reaction product is immersed in deionized water for ultrasonic cleaning, and then under a nitrogen condition, drying is conducted to obtain the biological stain-resistant coating with the long-acting super-hydrophilic performance. The method is simple to operate and mild in reaction condition, a prepared coating material has the polyphenol compound and a large amount of functional groups of carboxyl, phenolic hydroxyl, quinonyl, amido and the like, and the surface of the coating shows the long-acting super-hydrophilic performance. The adhesiveness betweenthe modified coating prepared by adopting the method and the base material is excellent, and the super-hydrophilic performance of the surface of the coating makes the material have an excellent biological stain-resistant function, so that the biological stain-resistant coating with the long-acting super-hydrophilic performance can be used for preparing materials of implanted devices, medical diagnosis instruments, medical sensors and the like.

The invention discloses a waterborne polyurethane leather coating agent with a biological stain and damage prevention function. According to a preparation method, firstly, through sulfydryl-double-bond clicking reaction, isoborneol monomers containing di-hydroxyls are synthesized; then, through the addition polymerization reaction, the isoborneol monomers are introduced into the polyurethane main chains; further, through the chain expansion reaction and the neutralization reaction, the waterborne polyurethane leather coating agent with the biological stain and damage prevention function is obtained. The isoborneol monomers have good biological stain and damage prevention performance; when the molecular weight is greater, the biological stain and damage prevention performance is better. Through the generation of addition polymerization reaction by diisocyanate and di-hydroxyls in the structure, the waterborne polyurethane leather coating agent can be introduced onto the polyurethane main chain in the molecule level. Good water-resistant performance and film forming performance of the polyurethane leather coating agent are maintained; meanwhile, the durable biological stain and damage prevention performance is realized.

The invention relates to an improved periodic acid Schiff (PAS) rapid staining kit. The kit comprises: a staining liquid, shedding-proof films, and cover slips. The staining liquid is a mixture of active fuchsine liquid and active fast green liquid, which is diluted with 95% of ethanol and 0.5% of sodium binitrite solution. The kit is advantaged in that: fuchsine and fast green are firstly prepared into a rapid biological rapid staining liquid; the staining process is fast, wherein the staining process takes 10 seconds; the detection process is simple, wherein a result can be observed directly, and washing is not required; the application scope is wide, wherein the kit can be used for staining various clinic specimens such as scurf, mucous and vaginal secretion. Periodic acid is attached on object slides, such that packing materials of the product is saved, and the operation process is simplified. The kit is especially suitable for a large clinic specimen amount. Because periodic acid, which is a flammable and combustible product, is attached to the object slides, periodic acid turns from liquid into a dried status. Therefore, danger of periodic acid is greatly reduced, and a standardized operation is easy to achieve. Poor repeatability due to personal operation errors can be avoided.

The invention relates to a liquid-dropping type automatic biological staining instrument; the top end of a machine frame is provided with a loading port; a vertical track extends downward to the interior of the machine frame from the loading port; a slide box is arranged at the loading port, can rise and fall up and down along the vertical track, and is internally provided with slides for bacteria staining; a transverse track is transversely arranged inside the machine frame; the rear end of a manipulator is in sliding connection on the transverse track, and the front end of the manipulator is used for clamping the slides in the slide box; a liquid-dropping type staining and cleaning device is arranged at the top end of the machine frame; a drying device is arranged inside the machine frame; a waste liquid cup is arranged at the bottom end of the machine frame and is used for containing a waste liquid of a staining liquid and a cleaning waste liquid; a control module is arranged on the side face of the machine frame, and is electrically connected with the manipulator, the liquid-dropping type staining and cleaning device and the drying device. Compared with the prior art, the liquid-dropping type automatic biological staining instrument has the advantages of simple operation, reliable method and stable performance, and can meet various staining ways.

The invention provides asymmetrical cyanine fluorescent dye expressed by formula I, wherein X, n, R1, R2, R3, R4 and Y are defined as a specification. The dye can be used as excellent nucleic acid staining agent, and the spectrum of the dye is in a near infrared region of 600 to 900 nanometers and has no interference of background fluorescence. Simultaneously, the dye can utilize a small red semiconductor laser as a light source (633 nanometers). The invention also provides a composition comprising the fluorescent dye and a method for performing biological stain by using the fluorescent dye or the composition thereof.

The invention discloses a polymeric compound coating and a preparation method and an application thereof. The polymeric compound coating disclosed by the invention has excellent corrosion resistant performance and biological stain resistance, and has a wide-spectral, efficient and lasting antibacterial effect. The preparation method disclosed by the invention is simple in process, few in environmental pollution and high in efficiency, spraying is not limited by dimension and shape of a workpiece, and the coating is low in cost and suitable for being prepared in a large area. The polymeric compound coating disclosed by the invention has good application prospect and economical benefit in the field of corrosion resistance and marine stain prevention.

The invention discloses 2, 5-di (rhodamine B-9'-(2''-benzoyl) radical)-1, 3, 4-diazole fluorescent stain compound I and a preparation method and application thereof. The compound I has excellent dissolubility in water, emits strong fluorescent light and can be applied to being biological stain, namely cell stain, and water solution of the compound I cannot suddenly extinct in fluorescence spectrum in a range of the pH (potential of hydrogen) value from 2 to 10. The compound I can be prepared from rhodamine B and phosphorus oxychloride directly or can be prepared through intermediate compound IIN and N'-double-rhodamine B hydrazine. The invention further discloses the application of the structural formula I serving as biological stain, namely cell stain.

The invention discloses preparation methods of prodigiosin and prodigiosin-containing biological stain as well as an application of the prodigiosin-containing biological stain in fabric. The preparation method of the prodigiosin comprises the following steps: (1), red pigment stains with the preservation number of CGMCC (China General Microbiological Culture Collection Center) 4074 are cultured in a microbial culture solution, and prodigiosin-containing fermentation broth is obtained; (2), the prodigiosin-containing fermentation broth is subjected to centrifugation and supernatant removal to obtain a precipitate, an organic solvent is added to the precipitate, the mixture is subjected to centrifugation, the organic solvent in supernatant is evaporated, and prodigiosin solids are obtained. The special microbial culture solution can improve the output of the prodigiosin, and industrial waste protein products can be adopted as raw materials to replace imported peptone used for fermentation production of the prodigiosin; according to a preferable technical scheme, the output of the prodigiosin can be further increased with adoption of mixed culture, and the antibacterial property is improved.

The invention discloses a water quality sensor. The sensor comprises a shell and an optical window arranged in the shell. The water quality sensor also comprises an ultraviolet light emitting source and a medium part which is used as the ultraviolet light transmitting medium. The ultraviolet light emitting source and the medium part are configured so as to make at least part of the ultraviolet lights emitted by the ultraviolet light emitting source are completely internally reflected in the optical window. The water quality sensor is provide with an ultraviolet light emitting source, which can emit ultraviolet lights to prevent and eliminate biological stains that may attach on the optical window. Besides, the ultraviolet lights are all confined in the optical window because of the light complete internal reflection, thus the total radiation strength is enhanced, so the water quality sensor can prevent biological pollution for a longer time, and the on-site continuous operation time of the water quality sensor is increased. The water quality sensor has the advantages of simple structure and low cost.

The invention belongs to the field of biomedical materials, and discloses moxifloxacin drug derivatives as well as a preparation method and application thereof. The moxifloxacin drug derivative has astructure shown as a formula I, wherein R is independently an organic cation of triphenylphosphine salt, pyridinium salt and quaternary ammonium salt; x is a monovalent anion; and n is any integer from 1 to 17. The invention also discloses a preparation method of the moxifloxacin drug derivative. The moxifloxacin drug derivative disclosed by the invention has an aggregation-induced emission property, can realize rapid staining recognition on microorganisms, and shows the characteristics of specificity, high efficiency and sensitivity. The moxifloxacin drug derivative disclosed by the inventionhas an excellent antibacterial effect. The moxifloxacin drug derivative disclosed by the invention is used for preparing reagents for specific dyeing of mitochondria and dyeing of microorganisms withnegative charges on the surfaces, and is also used for preparing reagents and antibacterial agents for distinguishing dead and living states of microorganisms.

The invention discloses a stable biological stain and a preparation method of same. The biological stain is composed of, by mass, 1-15% of trinitrophenol, 2-30% of sodium persulfate, 3-25% of acrylate, 1-15% of medical-grade acetic acid, 0.5-15% of 5,6,7,8-tetrahydrofolic acid, 0.1-5% of horseradish peroxidase, 1-10% of formalin, 1-30% of ethanol, and the balanced being deionized water. The preparation method includes the steps of: 1) fully mixing the deionized water with the trinitrophenol, the sodium persulfate and the medical-grade acetic acid, allowing the mixture to stand for precipitating, and collecting a supernatant; 2) mixing the supernatant with the acrylate, the 5,6,7,8-tetrahydrofolic acid, the horseradish peroxidase and the ethanol, and heating the mixture at 30-80 DEG C in water bath for 5-50 min, during the water bath heating process, the formalin is added; and 3) uniformly stirring the mixture to obtain the biological stain. The biological stain is low in usage amount,has good staining effect, can form clear colors, is easy to elute in later processes, is free of changing hardness of a sample and is high in sample completeness. The biological stain allows the sample to be quickly, high-effectively and accurately displayed in anatomy of biomedicine.

The invention relates to a dye and a preparation method thereof. The fluorescent dye is a series of novel dye which takes PnO2-PODIPY/PnO2-azaPODIPY developed from dipyrrin/aza-dipyrromethene complexing PnO2 (Pn-P, As, Sb, Bi) as a parent nucleus, and the general formula of the fluorescent dye is as shown in I/II. The fluorescent dye disclosed by the invention has the advantages of spectral property and the like of the classic traditional dye BODIPY/azaBODIPY. Although a central atom Pn is a sexadentate, the fluorescent dye is stable without being decomposed in air and a water solution. The compound has the outstanding characteristics of excellent water solubility and certain cell membrane permeability and can be used for biological dyeing. The novel dye disclosed by the invention has a novel spectral characteristic and can be applied to the aspect of fluorescence imaging.

The invention discloses a fibrilia biological dyeing technology based on laccase catalytic polymerization reaction. The fibrilia biological dyeing technology comprises the following steps: A, carryingout singeing treatment on fibrilia; B, immersing and treating singed fibrilia into an alkaline solution, and removing surface impurities and residual slurry; C, putting and repeatedly washing the fibrilia after alkaline treatment in clear water, and putting and drying the fibrilia in a low-temperature drying oven; D, immersing the fibrilia after the drying treatment in a buffer solution containing a phenols monomer and laccase, and carrying out biological dyeing; E, carrying out color fixing and steaming treatment on the fibrilia after the biological dyeing; F, finally carrying out soap boiling and water washing on the fibrilia. The fibrilia biological dyeing technology adopted by the invention is simple in operation and low in cost, is environment-friendly and pollution-free and is capable of improving dyeing and color fixing effects of the fibrilia.

The invention discloses a preparation method and a microscopic examination method of chitosan-embedded calcium alginate microcapsules. The preparation method comprises the following steps: mixing active microorganisms and a biological coloring agent according to a preset ratio to obtain a dyed active microorganism liquid; mixing soluble alginate, soluble carbonate, soluble calcium salt, water and the dyed lactic acid bacteria liquid according to a preset ratio, and performing primary embedding to obtain dyed calcium alginate microcapsule particles; adding the obtained dyed calcium alginate microcapsule particles into a chitosan solution to obtain a chitosan-embedded calcium alginate microcapsule subjected to secondary embedding; compared with the current situation that the chitosan-embedded calcium alginate microcapsule prepared by the existing method has higher requirements on the sample form and equipment, the chitosan-embedded calcium alginate microcapsule prepared by the preparation method has a certain color development effect, can be clearly observed under an optical microscope, has low requirements on the microscopic examination sample form, is low in microscopic examination equipment cost, and is suitable for industrial production. The microscopic examination process is fast and convenient, and the microscopic examination result is accurate.