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Application of oligomeric guluronic acid in the preparation of drugs, health products or dietary supplements for the prevention and treatment of alcoholic liver injury

A technology for polyguluronic acid and alcoholic liver injury, which is applied in the field of marine medicine, can solve the problems that have not been seen before, achieves broad development and application prospects, is easy to industrialize, and improves the effect of GSH level.

Inactive Publication Date: 2016-04-13
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are domestic and foreign literature reports that oligoguluronic acid has various biological activities such as anti-oxidation, immune regulation, and regulation of mucosal protein function, but there is no report on its application in alcoholic liver injury

Method used

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  • Application of oligomeric guluronic acid in the preparation of drugs, health products or dietary supplements for the prevention and treatment of alcoholic liver injury
  • Application of oligomeric guluronic acid in the preparation of drugs, health products or dietary supplements for the prevention and treatment of alcoholic liver injury
  • Application of oligomeric guluronic acid in the preparation of drugs, health products or dietary supplements for the prevention and treatment of alcoholic liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Preparation of different molecular weight oligomeric guluronic acids

[0023] Prepare polyguluronic acid (Mw=12.6kD, ie G-12K) into suspensions of different concentrations with pure water, and sonicate for 1min to fully dissolve it. Put it into 8 microwave extraction tanks (25mL per tank), set the power at 800W, and microwave it for different times under different temperature conditions (reaction conditions are shown in Table 1). After the product is cooled, filter through double-layer filter paper and freeze-dry , to obtain the guluronic acid oligosaccharide mixture, freeze-drying to obtain oligomeric guluronic acid with different molecular weights, namely G-3K, G-4K, G-5K, G-6K.

[0024] The structural formula of the prepared oligomeric guluronic acid is as follows: in the formula, R=H or Na, Li, Ca, Mg and other medicinal salts.

[0025]

[0026] where n is 1-60

[0027] Using TOSOHTSKGEL3000PWXL column chromatography (7.8mm*300mm, 6μm) and dextran...

Embodiment 2

[0031] Example 2: Inhibitory effect of oligomeric guluronic acid on alcohol-induced increase in mouse liver body mass index

[0032] 84 Kunming mice of 18-22g were randomly divided into control group, model group and administration group (different weight-average molecular weight oligomeric guluronic acid, Mw=3kD, Mw=4kD, Mw=5kD, Mw=6kD , Mw=12kD), 12 in each group. Adaptive feeding was carried out for 5 days. During the experiment period, the mice were free to eat common feed and water, 200 mg / kg was given once, 4.8 g / kg alcohol was given once 2 hours later, and water was not allowed. After 16 hours, the mice were sacrificed, the liver was separated, rinsed twice with pre-cooled PBS (0.1M, pH=7) solution, and then the water on the surface of the organ was blotted dry with filter paper, weighed, and the organ index was calculated. The calculation formula is as follows: organ index (%)=organ weight (g) / body weight (g)*100%, and the results are shown in Table 2.

[0033] Table...

Embodiment 3

[0038] Example 3: Inhibitory effect of oligomeric guluronic acid on alcohol-induced increase in serum TG and TC levels in mice

[0039] 60 Kunming mice of 18-22g were randomly divided into control group, model group, and administration group (different weight-average molecular weight oligomeric guluronic acid, Mw=3kD, M-5kD, Mw=12kD), each group 12 only. Adaptive feeding for 5 days. During the experiment period, the mice were free to eat common feed and drinking water. 200mg / kg was given once, 4.8g / kg alcohol was given once 2h later, water was not allowed to eat, and blood was taken from the eyeball after 16h, prepared at 3000rpm×10min Serum, determination of TG, TC indicators. as shown in Table 3.

[0040] Table 3 Inhibitory effects of different molecular weight oligomeric guluronic acids on the increase of TC and TG induced by alcohol

[0041]

[0042] ##P<0.01, compared with normal control group; *P<0.05, **P<0.01, compared with model group; (n=12; X±SD)

[0043] The...

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Abstract

The invention provides application of guluronic acid oligomers in preparing medicines, health products or dietary supplements for preventing and treating alcoholic liver injury. Guluronic acid oligomers with different molecular weights, which are obtained by degrading polyguluronic acid graded and purified from algin, can lower the hepatosomatic index increase caused by alcoholic injury, resist triglyceride and cholesterol increase caused by alcohol, effectively lower glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase increase in serum caused by alcohol, enhance the total oxidation resistance, glutathione level and superoxide dismutase activity in the serum, inhibit the generation of malonaldehyde in the liver caused by alcohol, lower the mouse drunkenness rate, prolong the mouse drunkenness time, and shorten the sober-up time, thereby implementing the protective action and hangover alleviating function on alcoholic hepatic injury. The products provided by the invention are derived from marine natural products, have the advantages of abundant resources, high industrialization tendency, high safety, high effectiveness and the like, and have wide development and application prospects in the aspects of prevention and treatment of alcoholic hepatic injury, hangover alleviation and liver protection.

Description

technical field [0001] The invention belongs to the field of marine medicines, and in particular relates to the application of oligomeric guluronic acid in the preparation of medicines, health care products or dietary supplements for preventing and treating alcoholic liver damage. Background technique [0002] Liver damage caused by excessive alcohol consumption is a worldwide medical problem. Alcoholic fatty liver (ALD) is an early manifestation of alcohol-induced liver damage, which can develop into alcoholic hepatitis, liver fibrosis, liver cirrhosis and even liver cancer. In developed countries such as Europe and the United States, alcoholic liver disease and its complications are one of the important causes of death. In recent years, the incidence of ALD in my country has also been increasing year by year, the degree of liver damage is aggravating, and the number of damaged functional organs is increasing, which has caused systemic harm to the health of the Chinese pop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7016A61K31/702A61K31/734A61K31/715A61P1/16A23L33/00
Inventor 李春霞管华诗胡婷郝杰杰李全才辛萌
Owner OCEAN UNIV OF CHINA
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