A kind of alginate lyase sha-3 gene and its prokaryotic expression vector

Abstract

The invention discloses an alginate lyase SHA‑3 gene, the nucleotide sequence of which is shown in SEQ ID NO: 1. The invention constructs a prokaryotic expression vector of the alginate lyase SHA‑3 gene, which can be used in The expression product alginate lyase SHA‑3 was obtained in a short period of time, and it has a wide range of substrate specificity. It can use both polymannuronic acid PolyM and polyguluronic acid PolyG as substrates, and the enzyme activity reaches 12U / mg, is a bifunctional enzyme with broad application prospects, the prokaryotic expression vector and the overall expression system of the present invention are easy to operate, and are convenient for industrial production.

Description

technical field

[0001] The invention belongs to the field of microbial genetic engineering, in particular to an alginate lyase SHA-3 gene and its prokaryotic expression vector pGEX-4T-1- SHA-3 , the vector highly expresses the alginate lyase protein SHA-3. Background technique

[0002] In recent years, the development and utilization of marine resources has gradually become a research hotspot. Because of its unique physical and chemical properties, alginic acid has broad application prospects in the fields of food, medicine and chemical industry. Alginate oligosaccharides have become the focus of new drug development because of their various physiological activities. At the same time, as one of the most abundant marine biomass, alginic acid has the following advantages: (1) high photosynthetic efficiency, fast growth, high yield, and abundant resources; (2) growth does not occupy arable land; (3) hardly contains wood The content of cellulose is very small, the pretreatment...

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