A kind of alginate lyase sha-2 gene and its expression vector

A technology of alginate lyase and gene, which is applied in the direction of genetic engineering, plant gene improvement, and the introduction of foreign genetic material using vectors, etc. It can solve the problems of low enzyme production, difficulty in meeting application requirements, and high cost of wild-type alginate-decomposing bacteria. problems, achieve broad substrate specificity, facilitate industrial production, and be easy to operate
CN104878031BActive Publication Date: 2018-07-24KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2018-07-24

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Abstract

The invention discloses an alginate lyase SHA‑2 gene, the nucleotide sequence of which is shown in SEQ ID NO: 1; the invention constructs a prokaryotic expression vector of the alginate lyase gene SHA‑2, the prokaryotic expression vector The expression product alginate lyase SHA‑2 can be obtained in a short period of time, and it has a wide range of substrate specificity, and can utilize both polymannuronate (Poly‑mannuronate, PolyM) and polyguluronic acid (Poly-guluronate, PolyG) is the substrate, and the enzyme activity reaches 5.2U / mg. It is a bifunctional enzyme with wide application prospects. The prokaryotic expression vector and the overall expression system of the present invention are easy to operate and convenient for industrial production.
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Description

technical field

[0001] The invention belongs to the field of microbial genetic engineering, in particular to an alginate lyase SHA-2 gene and its prokaryotic expression vector pGEX-4T-1- SHA-2 , the vector highly expresses the alginate lyase protein SHA-2. Background technique

[0002] In recent years, the development and utilization of marine resources has gradually become a research hotspot. Because of its unique physical and chemical properties, alginic acid has broad application prospects in the fields of food, medicine and chemical industry. Alginate oligosaccharides have become the focus of new drug development because of their various physiological activities. At the same time, as one of the most abundant marine biomass, alginic acid has the following advantages: (1) high photosynthetic efficiency, fast growth, high yield, and abundant resources; (2) growth does not occupy arable land; (3) hardly contains wood The content of cellulose is low, the pretreatment is sim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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