An endo-type bifunctional alginate lyase aly2 producing multiple monosaccharide products, its encoding gene and application

A technology of alginate lyase and dual function, which is applied in the field of endo-type bifunctional alginate lyase Aly2 and its coding gene, and can solve the problem that no endo-type alginate lyase has been found
CN108048435BActive Publication Date: 2021-05-25SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2021-05-25

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Abstract

The invention relates to an endo-type bifunctional alginate lyase Aly2 producing multiple monosaccharide products, its coding gene and application. The amino acid sequence of Aly2 is shown in SEQ ID NO.2, and the nucleotide sequence encoding Aly2 is shown in SEQ ID NO.1. The specific activities of the genetically engineered recombinant protein rAly2 prepared by the invention to alginate, polyguluronic acid and polymannuronic acid are 2025U / mg, 3672U / mg and 1324U / mg respectively, so it is a dual-functional enzyme. This enzyme is an endonuclease. When the alginate polysaccharide substrate is completely degraded, the final main products are unsaturated disaccharides and unsaturated trisaccharides; when degrading different oligosaccharide substrates, it can also produce saturated M and G or unsaturated Δ and other monosaccharide products. In addition, rAly2 has stable biochemical properties, so it is a potential tool enzyme, which can be used in the fields of medicine and food, and has broad application prospects.
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Description

technical field

[0001] The invention relates to an endo-type bifunctional alginate lyase Aly2 capable of producing various monosaccharide products, its coding gene and application, and belongs to the technical field of genetic engineering. Background technique

[0002] Alginate is an important acidic polysaccharide composed of β-D-mannuronic acid (β-D-Mannuronate, M) and α-L-guluronic acid (α-L-Guluronate, G) arranged alternately Linear anionic polysaccharides formed sequentially through β-1,4 glycosidic linkages. In the alginate molecule, these two sugar units are usually randomly arranged and combined into polymannuronate (PM), polyguluronate (PG) or glycerin / paleoalternating blocks [1] . Natural alginate mainly comes from macroalgae, sargassum, kelp and other large marine brown algae with the same source of medicine and food. As a structural component of marine brown algae, the content of alginate can reach more than 40% of the dry weight. In addition, some bacteria, ...

Claims

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