96results about How to "Potential for industrial applications" patented technology

The invention discloses a method for producing fuel oil by biomass hydrothermal liquefaction. The method is characterized by comprising the following steps: fully mixing a biomass raw material and solvent water (or adding catalyst water solution) to prepare seriflux, wherein a mass ratio of a catalyst to the raw material is 1:10 to 1:50, and the mass ratio of the raw material to water is 1:2 to 1:8; performing a reaction in a slurry bed reactor under a reducing atmosphere; controlling a liquefaction reaction temperature between 300 and 450 DEG C, a reaction pressure between 5 and 30 MPa and reaction time between 5 and 40 minutes; after the reaction, separating a product to obtain the fuel oil, solid residue, water, gas and the like. The method has the advantages of achieving complete biomass conversion, high oil product productivity, obtaining an oil product with a heat value equal to that of standard oil, relieving dependence on fossil energy, contributing to environment protection, lowering production cost and achieving good social and economic benefits.

The invention relates to alginate lyases, and in particular, relates to coding genes and applications of three algin-degrading alginate lyases. The alginate lyases comprise alginate lyases AlgAT0, AlgAT1 and AlgAT5 respectively. The coding genes of the alginate lyases have the base sequences of SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively. The alginate lyase genes are cloned into escherichia coli and pichia pastoris by a genetic engineering method. The AlgAT0, AlgAT1 and AlgAT5 are cloned into an expression vector of pichia pastoris; after fermentation condition optimization, fermentation is performed for 120 h, the concentrations of extracellular proteins are 0.312 g / L, 1 g / L and 9.39 g / L respectively, and the enzyme activities are 64666.67 U / mL, 126666.67 U / mL and 136025.6 U / mLrespectively. An escherichia coli recombinant strain and a pichia pastoris strain capable of producing alginase are obtained. The recombinant enzymes have stable properties and can be used for algin conversion with high added value, the enzyme activities of the three enzymes are much higher than values those reported so far, and the three enzymes have quite good potential for industrial application.

The invention discloses three-dimensional functionalized graphene for supercapacitors and a preparation method of the three-dimensional functionalized graphene. Natural graphite powder is used as a raw material for preparing graphite oxide, hydrochloric acid assists in ultrasonic stripping so that oxidized graphene can be prepared, and the three-dimensional functionalized graphene is obtained through controllable thermal reduction. The three-dimensional functionalized graphene prepared through the method is of a controllable multistage mutual through hole structure and has functionalized surface functional groups. The three-dimensional functionalized graphene is made into electrodes suitable for the supercapacitors, the specific capacitance value of the electrodes in a water-based electrolytic solution reaches 508 F / g, energy densities of the supercapacitors are 15 Wh / kg and 66 Wh / kg, and corresponding power densities of the supercapacitors are 14 kW / kg and 52 kW / kg in a water-based electrolytic solution system and an ionic liquid-based electrolytic solution system. When the power density of the ionic liquid-based electrolytic solution system is 128 kW / kg, the corresponding energy density still keeps 56 Wh / kg accounting for 85% that of an initial value.

The invention provides a method for preparing polyoxymethylene dimethyl ether carboxylate and / or methyl methoxy acetate which serves as an intermediate for producing ethylene glycol. The method comprises the step of enabling a raw material, namely polyoxymethylene dimethyl ether or methylal, together with carbon monoxide and hydrogen gas to react in a dealuminzation modified acidic molecular sieve catalyst loaded reactor under appropriate reaction conditions without adding other solvents, so as to prepare corresponding products, wherein a reaction process is of gas-liquid-solid three-phase reaction. According to the method provided by the invention, the conversion ratio of the raw material polyoxymethylene dimethyl ether or methylal is high, the selectivity of each product is high, the service life of a catalyst is long, external solvents are not required to be used, reaction conditions are relatively mild, and continuous production can be carried out, so that the method has industrial application potential. Furthermore, the obtained products can be used for producing ethylene glycol through hydrolyzing after hydrogenating or hydrogenating after hydrolyzing.

The invention relates to a method of preparing polyaryletherketone. The method comprises the following steps: enabling a bisphenol monomer and a salt-forming agent to react to generate a salt of bisphenol; and then, enabling the salt and 4,4'-dihalide diphenylketone to carry out polycondensation reaction to obtain the polyaryletherketone. The method is characterized in that the reaction of the formed salt of the bisphenol and the polycondensation reaction are carried out in the presence of an ionic liquid of a component (B) or composite ionic liquid containing a component (A) and the component (B), which is taken as a solvent, wherein the ionic liquid of the component (A) is selected from amphoteric ionic liquid of at least one compound with formulas I and II; and the ionic liquid of the component (B) is selected from ionic liquid of at least one compound with formulas III and IV; and each variable is defined in the specification of the application. By virtue of the method disclosed by the invention, not only can preparation conditions of polyaryletherketone be gentle, especially can reaction time (including water-carrying time) at a salt-forming stage be shortened, but also a great quantity of organic solvents do not need to be used for lowering viscosity.

The invention discloses a method for preparing bio oil through delignification pretreatment of biomass and liquefaction. In the method, biomass is subjected to delignification pretreatment and then liquefaction reaction in the presence of a solvent and a catalyst so as to prepare the bio oil. The method comprises the following steps of: performing delignification pretreatment on the biomass by using a chemical delignification agent or a biological method or mechanically performing delignification pretreatment on the biomass; and performing high-pressure liquefaction reaction on the biomass subjected to delignification pretreatment in the presence of a solvent and a catalyst or in the absence of the catalyst to prepare the bio oil. Compared with a method for preparing the bio oil by liquefying the biomass not subjected to delignification pretreatment, the method for preparing the bio oil by performing delignification pretreatment on the biomass and liquefying under the equal condition has the advantage that the yield of the bio oil is improved obviously.

The invention relates to incision-type sodium alginate lyase as well as an encoding gene and application thereof. The amino acid sequence of the incision-type sodium alginate lyase AlgL-5 is as shown in SEQ ID NO.2. A gene for encoding the incision-type sodium alginate lyase AlgL-5 is as shown in SEQ ID NO.1. Main oligosaccharide products generated in a process of degrading sodium alginate by using the incision-type sodium alginate lyase AlgL-5 include unsaturated disaccharide, unsaturated trisaccharide, unsaturated tetrasccharide and unsaturated pentasaccharide. Pentasaccharide is the smallest unsaturated oligosaccharide substrate of lyase, and disaccharide is the smallest unsaturated oligosaccharide product of lyase. The recombinant enzyme is stable in property and has certain industrial application potential.

The invention relates to an excision-type agarose, coding gene and application thereof. The excision-type agarase AgaO has an amino acid sequence shown as SEQ ID NO.2. The gene coding the excision-type agarase AgaO has nucleotide sequence shown as SEQ ID NO.1. In the degradation process of the agarose AgaO, the oligosaccharide main product is always noagarobiose; and the agarose has stable property and the potential for industrial application.

The invention discloses a method for producing fuel oil from biomass through hydro-thermal liquefaction. The method is characterized by comprising the following steps: fully mixing a biomass raw material with the solvent water (or further with an aqueous solution of a catalyst) to form slurry, wherein a mass ratio of the catalyst to the raw material is in a range of 1: 10 to 1: 50, and a mass ratio of the catalyst to water is in a range of 1: 2 to 1: 8; carrying out a reaction in a slurry bed reactor in a reducing atmosphere, wherein a liquefaction reaction temperature is controlled to be 300to 450 DEG C, a reaction pressure is controlled to be 5 to 30 MPa, and reaction time is controlled to be 5 to 40 min; and after completion of the reaction, carrying out separation on a product so as to obtain fuel oil, solid residues, water, gas, etc. According to the invention, the conversion of biomass is thorough; the yield of an oil product is high; the calorific value of the prepared oil product is equivalent to the calorific value of standard oil; and the method can alleviate dependence on fossil energy, is beneficial for environmental protection and low in production cost, and has goodsocial and economic benefits.

The invention relates to the field of genetic engineering technology, especially to alginate lyase and a preparation method and application thereof. Maturation protein of the alginate lyase contains an amino acid sequence as shown in the SEQ ID NO. 1, and the precursor protein contains an amino acid sequence as shown in the SEQ ID NO.4. The alginate lyase has stable properties, and specific enzyme activity reaches up to 4600 U / mL and above. Alginate can be subjected to enzymolysis to obtain micromolecular alginate-derived oligosaccharides with special biological activity. The product has the potential for industrial application.

The invention, relating to the field of cell manufacturing and energy storage, discloses a surface modification method for raising the activity of an electrode material of a vanadium cell, comprising the following steps: firstly cleaning the electrode material of the vanadium cell to remove surface impurities, then carrying out modification treatment of the electrode material by using plasma, reacting the gas which generates plasma with the electrode surface under ionization state to generate polar functional groups; and finally carrying out ultrasonic cleaning on the modified electrode material for 5-30 min and drying at 60-120 DEG C. According to the invention, by using the method of the invention to carry out surface modification on the electrode material of the vanadium cell, the hydrophilcity of the electrode surface is enhanced, partial polar functional groups has good catalytic influence on electrode reaction, and the energy storage efficiency of the cell is expected to increase. The method can be used for processing material surfaces with various morphologies and can keep mechanical properties and the like of material matrixes, the experiment condition are easy to control and the method has no pollution to environment. The method is efficient and environmentally friendly.

The present invention provides a method for preparing biological carbon through high pressure liquefaction of biomass. The method comprises that: the biomass and solvents are mixed and subjected to a liquefaction reaction for 0-80 minutes in the protection of inert atmosphere to obtain a liquefied product, wherein the reaction temperature is controlled to 180-450 DEG C, the reaction pressure is controlled to 1-20 MPa; the liquefied product is subjected to extraction and separation to respectively obtain the water-soluble organic product and the insoluble product; the insoluble product is subjected to extraction and separation to obtain the solid product, which is the biological carbon. The liquefaction reaction can be performed in the presence of a catalyst, wherein the catalyst comprises an acidic catalyst, a basic catalyst, a salt catalyst and a molecular sieve catalyst. The calorific value of the prepared biological carbon provided by the present invention is up to 20-33 MJ / Kg, and is equivalent to the calorific value of coal, such that the prepared biological carbon provided by the present invention can be adopted as a substitute of the natural coal.

The invention discloses a photothermal-chemotherapy combined therapeutic agent based on Prussian blue and a preparation method of the therapeutic agent. The therapeutic agent is characterized in that the therapeutic agent is of a liposome-like structure, Prussian blue nanoparticles are used as a core, a medicine-carrying bimolecular layer is formed outside the core through hydrophobic-hydrophobic interaction between two different amphipathic surfactants, and anticancer medicine is encapsulated in the medicine-carrying bimolecular layer. The therapeutic agent combines Prussian blue with the anticancer medicine to realize photothermal-chemotherapy synergistic treatment, so that the problem that chemotherapy medicine is low in specificity and human body utilization rate is solved, the defect that photothermal treatment is incomplete is overcome, treatment efficiency is improved greatly, and the therapeutic agent has good clinical application prospect.

The invention relates to a method for treating incineration fly ash by using a plasma fluidized bed. The method comprises the following steps: putting incineration fly ash to be treated into a plasma device which comprises the plasma fluidized bed and a high-voltage power supply; introducing carrier gas through a gas inlet; starting the high-voltage power supply; and forming disruptive discharge in a minimum-distance part between electrodes by virtue of knife-shaped stainless steel electrodes in the presence of high-voltage power, driving the disruptive discharge to quickly slide downstream along the surfaces of the electrodes by the carrier gas to form non-thermal arc plasma, converting the carrier gas into active gas containing a large quantity of charged particles, excited species, free radicals, electrons and photons, driving the incineration fly ash in a stainless steel mesh to be in a fluidized state by the active gas passing through the conical stainless steel mesh so as to form the plasma fluidized bed, and degrading and mineralizing persistent organic pollutants in the incineration fly ash to form inorganic matter by processes of diffusing, adsorbing, migrating and chemically reacting plasma by virtue of the active gas, thereby achieving the purpose of treating the incineration fly ash.

The invention discloses a method for preparing chiral monohydroxy derivatized 1,2-alcohol compounds by biocatalysis of a carbonyl reductase. Specifically, the carbonyl reductase ChKRED12, a substrate,a cofactor, glucose dehydrogenase, glucose and a buffer solution are mixed in a certain proportion and subjected to a reaction at pH of 7-8 and temperature of 20-40 DEG C, and (2S, 3S)-configurationproducts can be obtained. Compared with the prior art, the adopted method has the advantages of easy-to-prepare catalyst, mild reaction conditions, simple reaction system, no side reaction and high optical purity of the obtained products, and has great industrial application potential.

The invention relates to incision difunctional alginate lyase Aly2 generating various monosaccharide products, an encoding gene of the Aly2 and an application of the Aly2. The amino acid sequence of the Aly2 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the encoded Aly2 is as shown in SEQ ID NO. 1. The specific activity of prepared gene engineering recombinant protein rAly2 for alginate, polyguluronic acid and polymannuronic acid is 2025U / mg, 3672U / mg and 1324U / mg, so that the gene engineering recombinant protein rAly2 is a difunctional enzyme. The enzyme is an incision enzyme, and final main products are unsaturated disaccharide and unsaturated trisaccharide when an alginate polysaccharide substrate is thoroughly degraded. When different oligosaccharide substrates are degraded, various monosaccharide products such as saturated M and G or unsaturated delta can be generated. Besides, the rAly2 is stable in biochemical property, is a potential tool enzyme, can be applied tothe field of medicines, food and the like and has a wide application prospect.

The invention provides a recombinant escherichia coli for synthesizing 2'-fucosyllactose and construction method thereof, and the recombinant Escherichia coli is obtained by knocking out a beta-galactosidase gene in the genome of Escherichia coli, synthesizing a key enzyme gene of colanic acid from guanosine diphosphate fucose, enhancing expression key enzyme genes of lactose permease genes and a guanylate diphosphate fucose denovo synthesis route and expressing exogenous fucosyltransferase genes. According to the scheme, lactose hydrolysis can be reduced, and the rate of transferring exogenous lactose into cells is increased, so that the concentration of lactose in the cells is effectively increased; meanwhile, the consumption of the guanosine diphosphate fucose is reduced, and the concentration of the guanosine diphosphate fucose in cells is effectively improved; through codon optimization, the expression of an exogenous fucosyltransferase gene is improved, and synthesizing of the 2'-fucosyllactose is effectively promoted.

The invention relates to an M-preferred monosaccharide exo-algin lyase Aly-6 as well as a coding gene and application thereof. An amino acid sequence of the M-preferred monosaccharide exo-algin lyaseAly-6 is shown in SEQ ID NO. 2; and a nucleotide sequence for encoding the Aly-6 is shown in SEQ ID NO. 1. The invention discloses for the first time that an M-preferred monosaccharide exo-algin lyaseAly-6 is obtained from a genome of the flammeovirga yaeyamensis MY04. The enzyme is a monosaccharide exo-algin lyase and has an obvious M-preferred feature; the enzyme Aly-6 can continuously degradethe guluronic acid fragment from the algin in a monosaccharide excision manner, and can also continuously degrade the mannuronic acid fragment in a monosaccharide excision manner; and the enzyme Aly-6is easier to degrade the mannosolic acid fragment. The sequence characteristics, substrate degradation pattern and oligosaccharide formation characteristics of the enzyme are significantly differentfrom the sequence characteristics, substrate degradation pattern and oligosaccharide formation characteristics of the existing known algin lyase. The physical and chemical properties are stable, the activity is high, and the potential for industrial application is appreciable.

The invention relates to a method for fermentation production of yeast single cell protein by utilization of blue algae, which belongs to the technical field of blue algae treatment. The method adopts Saccharomyces cerevisiae as a fermentation strain and takes blue algae raw materials as a nitrogen source to perform fermentation production of the yeast single cell protein, researches the influence of a carbon source, vitamin, trace elements, mineral elements, culture temperature, inoculum concentration, initial pH, dissolved oxygen and the like on the yield of yeast, and optimizes the fermentation condition, so as to finally ensure that the yeast yield reaches 6.2 grams per liter with 1 percent (dry weight) of a blue algae culture medium. The invention provides a novel method for utilizing the blue algae in the Taihu Lake with industrial application potential.

The invention discloses two pseudomonas rhodesiae engineering bacteria and application thereof in preparation of 2, 5-furandicarboxylic acid. The 2, 5-furandicarboxylic acid is prepared by constructing a mixed bacteria multi-cell catalytic system by constructing genetic engineering bacteria of pseudomonas rhodesiae and oxidizing 5-hydroxymethylfurfural as a biocatalyst. The pseudomonas rhodesiae engineering bacteria and the application thereof in preparation of the 2, 5-furandicarboxylic acid have the advantages that (1) the mixed bacteria multi-cell catalytic system is adopted for the first time, and FDCA is synthesized by a one-pot method; and (2) the FDCA synthesis process is simple and controllable, three-step continuous oxidation reaction is carried out in the same system, the reaction system is simple, the reaction conditions are mild, expensive cofactors are not needed, intermediate oxidation products are not accumulated, the yield and the productivity of the FDCA are high, and the industrial application potential is achieved.

The invention discloses a preparation method of a high temperature heat storage material for solar photo-thermal power generation, which comprises the following steps of: with polyaluminum chloride as a main raw material, heating and stirring to dissolve out fused salt and form a precursor of the heat storage material, drying, roasting and cooling to obtain the heat storage material. The latent heat of phase change of the material is 35.5-80.7 J.g-1; and the phase-transition temperature range of the material is 492-797 DEG C. The preparation method of the heat storage material is characterized by simple process, simple and easy available raw materials, in-situ preparation and the like and provides the candidate heat storage material with industrial application potential for the solar photo-thermal power generation.

The invention relates to a device for treating organic contaminated soil on a plasma fluidized bed. The device comprises the plasma fluidized bed and a high voltage power supply, wherein the plasma fluidized bed consists of an air inlet, a quartz container, a tetrafluoroethylene base, knife-shaped stainless steel electrodes, a cone-shaped stainless steel net, the organic contaminated soil, a quartz cover and an exhaust port; the cone-shaped stainless steel net is arranged at the central axis at the top of the quartz container, and is fixed directly above the electrodes. The organic contaminated soil to be treated is put in the cone-shaped stainless steel net, a carrier gas is introduced, the high voltage power supply is started, and a non-thermal electric arc plasma is quickly formed between the electrodes under the driving of the carrier gas, so that the carrier gas is converted into active gas containing active species, the organic contaminated soil is driven to form a fluidization state, the plasma fluidized bed is formed, organic pollutants in the soil are degraded and mineralized through a plasma chemical reaction, and the purpose of repairing the soil is reached. The device is combined with double advantages of the plasma and the fluidized bed, and is a new technique with industrial application potential.

The invention relates to chondroitin sulfate lyase and an encoding gene and application thereof. The chondroitin sulfate lyase enCSase is separated from Photobacterium sp. QA16, an amino acid sequenceis shown as SEQ ID NO.2, and a nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The chondroitin sulfate lyase enCSase is a novel CSase ABC enzyme, has relatively high enzyme activity on chondroitin sulfate, dermatan sulfate and hyaluronic acid, and is in an incision enzyme mode when being used for degrading chondroitin sulfate, dermatan sulfate and hyaluronic acid; and the chondroitin sulfate lyase can be applied to preparation of chondroitin sulfate oligosaccharides, dermatan sulfate oligosaccharides and hyaluronic acid oligosaccharides, and has a wide application prospect inthe fields of medicines, foods and the like.

The invention discloses a method for producing an organic acid by anaerobic fermentation of an aquatic plant. The method comprises the steps of cleaning the aquatic plant, drying to constant weight, pulverizing, then putting the treated aquatic plant into a fermentation tank, adding acclimated fermentation sludge, adding water, and performing constant temperature fermentation to obtain fermentation liquid containing the organic acid. The method has the advantages of simplicity in operation, wide raw material source, low price, good fermentation effect and the like, so that the method is the one for producing the organic acid by fermentation with important industrial application potential.

The invention provides a Fe-based multifunctional catalyst. The Fe-based multifunctional catalyst is formed by coupling a Na-ZnFe@C catalyst and a K-CuZnAl catalyst, wherein the Na-ZnFe@C catalyst is a carbonized Fe-based metal organic framework material (ZnFe-MOFs) loaded with sodium ions, and the K-CuZnAl catalyst is a CuZnAl catalyst which is loaded with potassium ions. According to the method, the coupling mode of different catalytic active components of the multifunctional catalyst is optimized, reverse water-gas conversion and Fischer-Tropsch synthesis carbon chain growth in the reaction process are considered, high-selectivity synthesis of ethanol through CO2 one-step hydrogenation is achieved, the CO2 conversion rate reaches up to 39.2%, the ethanol selectivity is 35.0%, and the olefin selectivity is 33.0%; The efficient synergistic effect among different catalytic active components is realized, meanwhile, the invention develops a new catalytic reaction path for preparing ethanol by CO2 hydrogenation, and has higher economic value and social benefit.

The invention discloses application of a two-dimensional MXene film in separating water from isopropyl alcohol and belongs to the technical fields of preparation of two-dimensional nanofilms and liquid separation. The application comprises the following steps: firstly, putting the two-dimensional MXene film into a pervaporation device and adding an isopropanol aqueous solution into the raw material side; secondly, vacuumizing the penetration side by adopting a vacuum pump and introducing penetrated vapor into a cold trap for condensing; thirdly, collecting penetrating fluid in the cold trap and detecting the concentration of the penetrating fluid by using gas chromatography. According to the application of the two-dimensional MXene film in separating the water from the isopropyl alcohol, disclosed by the invention, the two-dimensional MXene film which has good mechanical properties and excellent separation properties is put into the pervaporation device for separating the water from the isopropyl alcohol; the two-dimensional MXene film has high selectivity and good chemical stability as well as industrial application potential in the field of liquid separation.

The invention relates to a method for modifying diatomite and immobilization of glucose isomerase containing cells. The method comprises the steps of performing fermentation culture on genetically engineered bacteria containing glucose isomerase genes to obtain wet cell masses, and performing suspension with a buffer solution to prepare bacterial suspension; and adding the modified diatomite to the bacterial suspension, performing uniform stirring and mixing, adding polyethyleneimine, then adding glutaraldehyde, performing cross-linking, performing stirring at 15-35 DEG C for cross-linking for0.5-5h, then performing sucking filtration, washing filtration cakes with distilled water, and then performing crushing into granules so as to obtain an immobilization glucose isomerase preparation containing glucose isomerase. The immobilization method provided by the invention has the advantages of being low in cost of immobilization materials, simple to operate, and high in mechanical strength, prepared immobilization recombinant escherichia coli whole cells generating the glucose isomerase are used for catalyzing D-glucose to generate D-levulose, and under the condition of 60 DEG C, a reaction in batches is performed, and after 40 batches are reused, 86% or above of enzyme activity also exits. Through a continuous reaction for 603h, the time and space yield is 3.8kg / L / d, the average conversion rate is greater than 42%, and the method has favorable industrial application prospects.

The invention relates to a preparing method of a flexible carbon nanotube / metal composite film applied to the electromagnetic shielding field, and relates to a method for improving the existing nanotube film electromagnetic shielding effectiveness by growing metal particles on the carbon nanotube film surface. The preparing method sequentially comprises the following steps that firstly, the surface morphology of the carbon nanotube film is constructed; secondly, electrodeposition liquid is prepared; thirdly, a cathode deposition component is assembled; and fourthly, the metal particles are grown on the surface of the carbon nanotube film. The carbon nanotube film obtained by the method has high conductivity, excellent flexibility and light weight, and the adopted method and equipment are simple, cost is low, pollution is avoided, repeated use can be achieved, and the electrodeposition process can achieve the characteristics of industrialization and the like.

The invention relates to analdo-keto reductase, and application of recombinant expression transformant of the aldo-keto reductaseas a biocatalyst in asymmetric reduction of 2-carbonyl-4-phenylbutanoate to synthesis (R)-2-hydroxyl-4-phenylbutyrate, and belongs to the technical field of bioengineering. The amino acid sequence of the aldo-keto reductase is shown in SEQ ID NO.1. The encodinggene the aldo-keto reductase corresponds to the nucleotide sequence of the amino acid sequence of the SEQ ID NO.1 and is shown in SEQ ID NO.2. The novel aldo-keto reductaseis high in catalytic performance.

The invention relates to an endo-beta-manna hydrolase Man01929 and a method for mutating the endo-beta-manna hydrolase Man01929 into glycosyl transferase and application of the endo-beta-manna hydrolase Man01929; an amino acid sequence of the endo-beta-manna hydrolase Man01929 is shown by SEQ ID NO.2; and a nucleotide sequence encoding the Man01929 is shown by SEQ ID NO.1. Site-directed mutation is performed on the hydrolase, and a result shows that 118, 119, 124 and 323 sites of the amino acid sequence play an important role in the substrate selective action process of recognition, binding, degradation and the like of the Man01929 on galactomannan, and the shown result has reference value for promoting research of the substrate selectivity mechanism of GH5 family beta-mannase. In addition, the invention further obtains a mutant D124Y with transglycosylation ability, and thus, an important reference is provided for revealing the transformation mechanism of the catalysismechanism of transforming the hydrolase into the glycosyl transferase.