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Method for producing organic acid by anaerobic fermentation of aquatic plant

An aquatic plant, anaerobic fermentation technology, applied in the direction of fermentation, can solve problems such as high energy consumption and unstable fermentation process, and achieve the effects of simple and easy method, important industrial application potential, and important ecological benefits

Active Publication Date: 2014-11-05
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, high-temperature fermentation consumes a lot of energy, and the fermentation process is unstable

Method used

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  • Method for producing organic acid by anaerobic fermentation of aquatic plant
  • Method for producing organic acid by anaerobic fermentation of aquatic plant
  • Method for producing organic acid by anaerobic fermentation of aquatic plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1: Preparation of fermented sludge.

[0092] The fermented sludge used in the experiment was taken from the dehydrated residual sludge of a sewage treatment plant.

[0093] Prepare acclimatization medium with the following components: glucose 15g / L, NaNO 3 3.04g / L, KH 2 PO 4 0.44g / L, MgSO 4 ·7H 2 O 0.96g / L, CaCl 2 0.72g / L, NaHCO 3 0.96g / L, MnCl 2 0.11g / L. Then put 2.5kg of dehydrated residual sludge from the sewage treatment plant into a 5L fermenter, add 4L of acclimatization medium, adjust the pH to 7.4, acclimate at 28°C for one week, and monitor and control the pH every day.

[0094] The activated sludge in the following examples were all prepared according to the above method.

Embodiment 2

[0095] Example 2: Influence of wetland plant type on fermentation effect.

[0096] Weigh a certain amount (according to 30g dry weight) of fresh cattail stalks, Zailihua stalks, bitter grass, and weed grass, put them into a 500ml Erlenmeyer flask after crushing, add 300ml of domesticated fermented sludge (sludge concentration MLSS10000mg / L), add 200ml of water, adjust the pH value to 7-8, and place it in a thermostat at 37°C for fermentation. The test lasted for 2 weeks. On days 0, 1, 2, 4, ..., 14, take 15ml of fermentation broth, centrifuge at 12000rmp for 5 minutes, take the supernatant, and measure TN and NH in part 3 -N, TP, COD Cr , pH, and another 4ml was passed through a 0.22μm water filter membrane to measure volatile fatty acids (VFAs).

[0097] The release of nitrogen and carbon sources during the fermentation process of four constructed wetland plants, Cattail, Zailihua, Erythrophyllum, and Smilax, is as follows: Figure 1 ~ Figure 3 As shown, the maximum outpu...

Embodiment 3

[0105] Embodiment 3: the influence of temperature on the fermentation effect of Smilax chinensis.

[0106] The experimental method refers to Example 2, the difference is that, according to the results of fermentation experiments of different types of wetland plants, the submerged water plant Smilax chinensis with the best fermentation effect is selected as the object, and the effects of different temperatures (10°C, 25°C, 37°C) on fermentation are investigated. The impact of the effect.

[0107] At three fermentation temperatures of 10°C, 25°C, and 37°C, the TN, TP, and COD Cr and VFAs content changes with fermentation time as Figure 6-9 shown.

[0108] Depend on Figure 9 It can be seen that the fermentation temperature has a significant effect on the nitrogen release rate and release amount of Smilax. The order of release rate and release amount of Nitrogenin from large to small under the three fermentation temperatures was 37℃>25℃>10℃. At 37°C, the release of nitrogen...

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Abstract

The invention discloses a method for producing an organic acid by anaerobic fermentation of an aquatic plant. The method comprises the steps of cleaning the aquatic plant, drying to constant weight, pulverizing, then putting the treated aquatic plant into a fermentation tank, adding acclimated fermentation sludge, adding water, and performing constant temperature fermentation to obtain fermentation liquid containing the organic acid. The method has the advantages of simplicity in operation, wide raw material source, low price, good fermentation effect and the like, so that the method is the one for producing the organic acid by fermentation with important industrial application potential.

Description

technical field [0001] The invention belongs to the application field of waste biomass, and in particular relates to a method for producing organic acids by fermentation of aquatic plants. Background technique [0002] In recent years, studies have found that urban organic wastewater (such as brewing wastewater, molasses wastewater, starch wastewater, etc.) It is preferentially used by denitrifying microorganisms. [0003] Anaerobic fermentation refers to the process of biodegrading organic matter under the condition of being isolated from the air, relying on the biochemical action of facultative anaerobic bacteria and obligate anaerobic bacteria, also known as anaerobic biological treatment or anaerobic digestion. , It is mainly used to treat high-concentration organic industrial wastewater, sludge from urban sewage, animal and plant residues, and feces. The anaerobic digestion process of complex organic matter goes through several stages, which are successively completed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12P7/52C12P7/54
Inventor 杨柳燕陈乾坤焦一滢张权李丽陈旭
Owner NANJING UNIV
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