The invention discloses a polypeptide and plant composition for inhibiting melanin synthesis and a preparation method of the polypeptide and plant composition. The polypeptide and plant composition comprises palmitoyl pentapeptide-4, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, palmitoyl tripeptide-5, nonapeptide-1, L-carnosine, hexapeptide-9, acetyl hexapeptide-8, pentapeptide-3, a coix seed extract, a cynomorium songaricum extract, an astragalus membranaceus extract, a radix bupleuri extract and a liquorice root extract. According to the invention, the polypeptide and the plant composition are compounded, and different action mechanisms and sites of the polypeptide and the plant composition are utilized to perform whitening, repairing and anti-aging on the skin from multiple aspects. The polypeptide and the plant extract have a synergistic effect, and along with the increase of the variety of the polypeptide and the addition of the plant extract, the promotion effect on fibroblast proliferation is improved. Meanwhile, the polypeptide components are homologous with human bodies, so that human immune response and other side effects are not easily caused.