Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lycium barbarum polysaccharide, Lycium barbarum polysaccharide and Lycium barbarum polypeptide produced by simultaneous separation and preparation method thereof

A technology of Lycium barbarum polysaccharides and Lycium barbarum, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of waste of raw material residues, decline in extraction rate and purity, and reduce the interference of impurities , wide application prospects, and the effect of improving the value of use

Active Publication Date: 2021-08-06
百瑞源枸杞股份有限公司 +1
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In summary, in the prior art, the extraction of Lycium barbarum polysaccharides, polypeptides, and Lycium barbarum is often carried out using Lycium barbarum as the starting raw material. During the extraction process, interference from other impurities will inevitably be received, resulting in a decline in the extraction rate and extraction purity. In the process of extracting effective substances from wolfberry as raw material, a lot of waste of raw material residues is often caused

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0047] Experimental example 1 Determination method of Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide

[0048] In the present invention, the determination method of Lycium barbarum polysaccharide is as follows: the determination of polysaccharide content in the sample refers to the SN / T4260-2015 method; the method of Lycium barbarum is: the determination of Lycium barbarum content in the sample is determined according to the established HPLC method; the method of Lycium barbarum polypeptide For: determined by using graphite digestion-semi-automatic nitrogen determination method.

[0049] 1. Determination of Lycium barbarum polysaccharides: using the method for the determination of reducing sugar content. Prepare a buffer solution, and the solution formula is to add 13.4 g of potassium sodium tartrate, 3,5-dinitrosalicylic acid, 1.8 g of sodium hydroxide, and 0.7 g of phenol per 100 mL of water. Use the glucose standard to draw a standard curve, ac...

Embodiment 1

[0057] Embodiment 1 Preparation of Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide

[0058] Using dried Lycium barbarum as raw materials, Lycium barbarum polysaccharides, Lycium barbarum, and Lycium barbarum active peptides were prepared according to the following process:

[0059] Using dried wolfberry as raw material, weigh 30g wolfberry and add 300ml deionized water to soak for 2 hours, crush it, ultrasonically extract it for 20 minutes, and centrifuge it at 5000g for 10 minutes. The supernatant is used to prepare wolfberry polysaccharide, and the precipitate is used to extract wolfberry red pigment and wolfberry peptides.

[0060] (1) Preparation of wolfberry polysaccharide

[0061] Add 540 ml of 90% ethanol to 270 ml of supernatant, let stand at 4° C. for 6 hours, centrifuge at 3000 g for 10 minutes, precipitate and freeze-dry to obtain 1.60 g of Lycium barbarum polysaccharide with a purity of 50.73% and an extraction rate of 5.34%.

[0062]...

Embodiment 2

[0067] The preparation of embodiment 2 Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide

[0068] Using dried wolfberry as raw material, weigh 20g wolfberry and add 220ml of deionized water to soak for 5 hours, then pulverize, ultrasonically extract for 40 minutes, and centrifuge at 5000g for 15 minutes to obtain 200ml of supernatant for preparing wolfberry polysaccharide, and precipitate for extraction Lycium barbarum and Lycium barbarum peptides.

[0069] (1) Preparation of wolfberry polysaccharide

[0070] Add 1000ml of 80% ethanol to the supernatant, let stand at 4°C for 9 hours, centrifuge at 4000g for 15 minutes, freeze-dry the precipitate, and obtain Lycium barbarum polysaccharide with a purity of 51.67% and an extraction rate of 6.98%.

[0071] (2) Preparation of Lycium barbarum

[0072] Lyophilize 19.1 g of precipitate obtained from preliminary extraction of wolfberry, and grind again to obtain wolfberry powder. Add 324 ml of organic solv...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses wolfberry polysaccharide, wolfberry red pigment, wolfberry polypeptide and a preparation method thereof which are simultaneously separated and prepared, and belongs to the technical field of wolfberry deep processing. In the present invention, Lycium barbarum is used as a raw material, and Lycium barbarum polysaccharide is obtained by soaking in water and extracted with ethanol. At the same time, the prepared Lycium barbarum residue is further added with an organic solvent for extraction to obtain Lycium barbarum red pigment, and the residue obtained in the previous step is continuously used by adding water and probiotics. , the polypeptide is prepared by microbial fermentation. In order to solve the problems of raw material waste and the like, the present invention maximizes the utilization value of Lycium barbarum. By using Lycium barbarum as raw material, Lycium barbarum polysaccharide, Lycium barbarum polysaccharide, Lycium barbarum polypeptide products are successively obtained at one time, and at the same time, Lycium barbarum polysaccharide, Lycium barbarum polysaccharide, Lycium barbarum The high-efficiency synergy of the continuous extraction of polypeptides not only improves the extraction efficiency and purity of each, but also effectively avoids the interference of other components on the extraction of the target components during the extraction process, and can be widely used in industrial production.

Description

technical field [0001] The invention belongs to the technical field of deep processing of wolfberry, and in particular relates to a synchronous separation and preparation of wolfberry polysaccharide, wolfberry red pigment, wolfberry polypeptide and a preparation method thereof. Background technique [0002] Lycium barbarum is a plant in the family Solanaceae. Lycium barbarum fruit is a traditional medicine and food homologous medicinal material in China. Lycium barbarum has a long history of consumption in my country. The "Dictionary of Chinese Medicine" records that wolfberry fruit has the effects of nourishing the liver and kidney, benefiting energy and improving eyesight. Modern biological research shows that wolfberry has various biological activities such as anti-oxidation, blood fat reduction, anti-aging, liver protection, etc. With people's attention to health, the consumption of wolfberry is increasing year by year. In order to meet the needs of different groups of pe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07C403/24C09B61/00C09B67/54C12P21/00C12R1/125C12R1/225C12R1/46
CPCC07C403/24C08B37/00C08B37/0003C09B61/00C09B67/0096C12P21/00C07C2601/16
Inventor 靳艳庄晶玲叶明亮张金宏郝向峰杨丽丽马奇虎姬宏礼
Owner 百瑞源枸杞股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products