Lycium barbarum polysaccharide, Lycium barbarum polysaccharide and Lycium barbarum polypeptide produced by simultaneous separation and preparation method thereof
A technology of Lycium barbarum polysaccharides and Lycium barbarum, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of waste of raw material residues, decline in extraction rate and purity, and reduce the interference of impurities , wide application prospects, and the effect of improving the value of use
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experiment example 1
[0047] Experimental example 1 Determination method of Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide
[0048] In the present invention, the determination method of Lycium barbarum polysaccharide is as follows: the determination of polysaccharide content in the sample refers to the SN / T4260-2015 method; the method of Lycium barbarum is: the determination of Lycium barbarum content in the sample is determined according to the established HPLC method; the method of Lycium barbarum polypeptide For: determined by using graphite digestion-semi-automatic nitrogen determination method.
[0049] 1. Determination of Lycium barbarum polysaccharides: using the method for the determination of reducing sugar content. Prepare a buffer solution, and the solution formula is to add 13.4 g of potassium sodium tartrate, 3,5-dinitrosalicylic acid, 1.8 g of sodium hydroxide, and 0.7 g of phenol per 100 mL of water. Use the glucose standard to draw a standard curve, ac...
Embodiment 1
[0057] Embodiment 1 Preparation of Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide
[0058] Using dried Lycium barbarum as raw materials, Lycium barbarum polysaccharides, Lycium barbarum, and Lycium barbarum active peptides were prepared according to the following process:
[0059] Using dried wolfberry as raw material, weigh 30g wolfberry and add 300ml deionized water to soak for 2 hours, crush it, ultrasonically extract it for 20 minutes, and centrifuge it at 5000g for 10 minutes. The supernatant is used to prepare wolfberry polysaccharide, and the precipitate is used to extract wolfberry red pigment and wolfberry peptides.
[0060] (1) Preparation of wolfberry polysaccharide
[0061] Add 540 ml of 90% ethanol to 270 ml of supernatant, let stand at 4° C. for 6 hours, centrifuge at 3000 g for 10 minutes, precipitate and freeze-dry to obtain 1.60 g of Lycium barbarum polysaccharide with a purity of 50.73% and an extraction rate of 5.34%.
[0062]...
Embodiment 2
[0067] The preparation of embodiment 2 Lycium barbarum polysaccharide, Lycium barbarum, Lycium barbarum polypeptide
[0068] Using dried wolfberry as raw material, weigh 20g wolfberry and add 220ml of deionized water to soak for 5 hours, then pulverize, ultrasonically extract for 40 minutes, and centrifuge at 5000g for 15 minutes to obtain 200ml of supernatant for preparing wolfberry polysaccharide, and precipitate for extraction Lycium barbarum and Lycium barbarum peptides.
[0069] (1) Preparation of wolfberry polysaccharide
[0070] Add 1000ml of 80% ethanol to the supernatant, let stand at 4°C for 9 hours, centrifuge at 4000g for 15 minutes, freeze-dry the precipitate, and obtain Lycium barbarum polysaccharide with a purity of 51.67% and an extraction rate of 6.98%.
[0071] (2) Preparation of Lycium barbarum
[0072] Lyophilize 19.1 g of precipitate obtained from preliminary extraction of wolfberry, and grind again to obtain wolfberry powder. Add 324 ml of organic solv...
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