A kind of retinoid nanoparticles and preparation method thereof

A technology of nano-particles and retinoids, which is applied in the field of new dosage forms and preparations of protein drugs, can solve the problems such as that the nano-particles of retinoids have not been reported in the literature, and achieves the effects of easy and stable preservation, high safety and good resolubility.

Active Publication Date: 2020-08-14
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the research on rehmannia glutinin nanoparticles has not been reported in the literature at home and abroad.

Method used

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  • A kind of retinoid nanoparticles and preparation method thereof
  • A kind of retinoid nanoparticles and preparation method thereof
  • A kind of retinoid nanoparticles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the extraction of rehmanniacin DHP1

[0032] Fresh rehmannia glutinosa is ground by a juicer, mixed with 0.2 mol / L, pH 7.0 phosphate buffer at a ratio of solid to liquid 1:3, placed at 4 °C, stirred and extracted for 24 hours; the obtained rehmannia glutinosa is filtered through 8 layers of gauze Extract the liquid, discard the filter residue, centrifuge the filtrate at 15,000 rpm for 20 min at 4°C, and collect the supernatant; under the condition of magnetic stirring at 4°C, slowly add anhydrous ammonium sulfate to the rehmannia extract to make the solution salt When the concentration reaches 20%, after it is completely dissolved, let stand at 4°C for 4 h, centrifuge at 12,000 rpm for 20 min, and collect the supernatant; continue to slowly add anhydrous ammonium sulfate to the collected supernatant until the saturation is 40%, After completely dissolving, collect the supernatant in the same steps as before; continue to add ammonium sulfate to the supernat...

Embodiment 2

[0035] Embodiment 2: the extraction of rehmanniacin DHP2

[0036] Rehmannia glutinosa is ground by a high-speed pulverizer, the ratio of solid to liquid is 1:3, and 0.2 mol / L, pH 7.0 phosphate buffer is added to mix evenly, placed at 4 °C, stirred and extracted for 24 hours; the obtained rehmannia glutinosa is filtered through 8 layers of gauze Extract the liquid, discard the filter residue, centrifuge the filtrate at 15,000 rpm for 20 min at 4°C, and collect the supernatant; under the condition of magnetic stirring at 4°C, slowly add anhydrous ammonium sulfate to the rehmannia extract to make the solution salt When the concentration reaches 20%, after it is completely dissolved, let stand at 4°C for 4 h, centrifuge at 12,000 rpm for 20 min, and collect the supernatant; continue to slowly add anhydrous ammonium sulfate to the collected supernatant until the saturation is 40% , after being completely dissolved, collect the supernatant in the same steps as before; continue to ad...

Embodiment 3

[0039] Embodiment 3: the preparation of rehmanniacin nanoparticles

[0040] The rehishin was prepared as a 1 mg / mL salt solution at pH 6.5, heated at 70°C for 1 hour, and the unreacted rehmanin was removed by TSK gelG6000PW gel chromatography to obtain rehmanin nanoparticles. The properties of rehmannia nanoparticle were studied by using dynamic light scattering technology, dynamic light scattering, multi-angle laser light scattering and scanning electron microscope. See the attachment for electron microscope observation pictures of rehmannia glutin nanoparticles Figure 9 .

[0041] The particle size and surface potential were measured with a laser particle size analyzer, and the average particle size was measured to be 120.61±0.46 nm, and the surface charge was negative, ranging from -18±5mV. Figure 10 .

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Abstract

The invention discloses radix rehmanniae proteins and a preparation method of radix rehmanniae protein nano-particles. Each particle is composed of two correspondingly proteins, wherein the N-end primary structural sequences of the two radix rehmanniae proteins are SEQ ID NO:1: R-E-K-D-I-A-G-A-V-Q-T-V and SEQ ID NO:2: R-E-K-D-I-V-X correspondingly. The radix rehmanniae proteins are extracted and separated from fresh radix rehmanniae, or raw radix rehmanniae or prepared radix rehmanniae and stably exist in the radix rehmanniae raw materials which are used traditionally. No cross-linking agent is involved in the preparation method of the radix rehmanniae protein nano-particles, finished products of the radix rehmanniae proteins are widely used in various decoctions prepared from the raw materials of the radix rehmanniae through a traditional hot processing technique, wherein the decoctions are taken by a large number of people for many years and are high in safety. The radix rehmanniae protein nano-particles are safe and efficient, the efficacy of the radix rehmanniae protein nano-particles is achieved safely and efficiently, and in addition, the radix rehmanniae protein nano-particles are expected to be combined with other raw materials, so that the effects are achieved efficiently and collaboratively.

Description

technical field [0001] The invention relates to a new dosage form and preparation technology of protein medicine in the technical field of medicine, in particular to a rehmannia protein nanoparticle and a preparation method thereof. Background technique [0002] In recent decades, nanoparticle research has been extensive. After nanomaterials, the physical and chemical properties (optical, electrical, surface activity, etc.) of nanomaterials will be completely different from those of the original materials, with targeting and penetrating properties. At the same time, it also exposes the disadvantages in biocompatibility, cytotoxicity and degradability. There are no natural research models for biological macromolecules (such as serum albumin, silk protein, etc.) currently used in the research of nano-drug carriers, and long-term observations are required to obtain the physicochemical properties of nanoparticles and their related biological safety. Comprehensive assessment. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61K9/19A61P39/06C07K14/415C07K1/36C07K1/30C07K1/20C07K1/18C07K1/16G01N30/02A61K36/804
CPCA61K9/19A61K36/804A61K38/168C07K14/415G01N30/02
Inventor 丁伟柯李晶周建武饶平凡
Owner ZHEJIANG GONGSHANG UNIVERSITY
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