Method for producing yeast single cell protein by blue algae fermentation

A single-cell protein and cyanobacteria technology, applied in the field of cyanobacteria treatment, can solve the problems of environmental pollution, high cost of burning cyanobacteria, and difficulty in cyanobacteria disposal.

Inactive Publication Date: 2009-03-18
JIANGNAN UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

After a large number of blue-green algae were salvaged, due to the limited land resources in Wuxi, it was difficult to dispose of blue-green algae, the utilization rate of biogas produced by fermentation of blue-green algae was low, and the cost of burning blue-green algae was high and polluted the environment

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  • Method for producing yeast single cell protein by blue algae fermentation
  • Method for producing yeast single cell protein by blue algae fermentation
  • Method for producing yeast single cell protein by blue algae fermentation

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Experimental program
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Embodiment 1

[0018] Embodiment 1: Determination of the best carbon source

[0019] The concentration of cyanobacteria salvaged from Taihu Lake is 2% (dry weight), and it is made into a culture medium after breaking the wall at high temperature. optimization. The carbon-nitrogen ratio in cyanobacteria is 5.5 / 1, and the carbon-nitrogen ratio required for yeast growth is 11.4 / 1, so 0.9% glucose can be added to 1% (dry weight) cyanobacteria medium to adjust the carbon-nitrogen ratio. The residual sugar content of 1% cyanobacteria medium was 0.26g, 0.172g after 12h fermentation, 0.075g after 16h fermentation, 0.069g after 20h fermentation, and 0.057g after 24h fermentation. Therefore, glucose can be added after 20 hours of cultivation. The amount of glucose added is 0%, 2%, 4%, and 6%. , the pH is 6, and the inoculum size is 10%. Adding 4% glucose has the highest yeast biomass, and the yeast cell mass can reach 3.8g / L when cultured for 36 hours. At this time, the residual sugar is 0.169g, an...

Embodiment 2

[0020] Embodiment 2: the determination of vitamins, trace elements and mineral elements in the culture medium

[0021] On the basis of carbon source optimization, vitamin mother liquor, trace element mother liquor and mineral element mother liquor were added to the medium to investigate their effects on fermentation. The results showed that the yeast yield reached the maximum after 36 hours of fermentation. The results are shown in Tables 1, 2, and 3. , it can be seen from the table that adding 1% of vitamin mother solution can significantly increase the biomass. After 36 hours of cultivation, the yeast yield reached 5g / L, but the addition of trace elements and mineral elements had no obvious effect on the yeast biomass.

[0022] Vitamin Mother Solution (g / L): Vitamin B 1 1. Nicotinic acid 0.4, pyridoxine 0.4, biotin 0.02, calcium pantothenate 2, riboflavin 0.2, inositol 1, p-aminobenzoic acid 0.2;

[0023] Table 1 Effects of different vitamin mother liquor additions on yeas...

Embodiment 3

[0029] Embodiment 3: Determination of optimal culture conditions

[0030] On the basis of medium optimization, the effects of fermentation temperature, initial pH value of medium and inoculation amount on fermentation results were investigated, and the results are shown in Tables 4, 5, and 6. It can be seen from the table that the optimum temperature for fermentation is 28°C, the initial pH value of the optimum medium is 6, and the optimum inoculum size is 15%.

[0031] Table 4 Effect of temperature on yeast biomass

[0032]

[0033] Table 5 Effect of pH value on yeast biomass

[0034]

[0035] Table 6 Effects of different inoculum sizes on yeast biomass

[0036]

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Abstract

The invention relates to a method for fermentation production of yeast single cell protein by utilization of blue algae, which belongs to the technical field of blue algae treatment. The method adopts Saccharomyces cerevisiae as a fermentation strain and takes blue algae raw materials as a nitrogen source to perform fermentation production of the yeast single cell protein, researches the influence of a carbon source, vitamin, trace elements, mineral elements, culture temperature, inoculum concentration, initial pH, dissolved oxygen and the like on the yield of yeast, and optimizes the fermentation condition, so as to finally ensure that the yeast yield reaches 6.2 grams per liter with 1 percent (dry weight) of a blue algae culture medium. The invention provides a novel method for utilizing the blue algae in the Taihu Lake with industrial application potential.

Description

technical field [0001] The invention relates to a method for fermenting and producing yeast single-cell protein by using cyanobacteria raw materials in Taihu Lake, and belongs to the technical field of cyanobacteria treatment. Background technique [0002] Blue algae (blue algae) is a kind of prokaryote with a long history of evolution, Gram-negative, no flagella, containing chlorophyll a, not forming chloroplast, and capable of aerobic photosynthesis. In recent years, as a large amount of pollutants have flowed into Taihu Lake, there have been frequent outbreaks of cyanobacteria in Taihu Lake. After a large number of blue-green algae were salvaged, due to the limited land resources in Wuxi, it was difficult to dispose of blue-green algae, the utilization rate of biogas produced by the fermentation of blue-green algae was low, the cost of burning blue-green algae was high, and it polluted the environment. Because cyanobacteria are rich in organic substances, which contain 5...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12R1/865
Inventor 杨海麟李克朗王武张玲
Owner JIANGNAN UNIV
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