Establishing and application of scale cancer cell line of purify species New Zealand mice oral cavaty and palatal surface parts

A cancer cell, face technology, applied in the field of biology, can solve the problem of not establishing stable large animal oral cancer cell lines and so on

Active Publication Date: 2006-11-15
郭伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no stable large animal oral cancer cell lines have been established

Method used

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  • Establishing and application of scale cancer cell line of purify species New Zealand mice oral cavaty and palatal surface parts
  • Establishing and application of scale cancer cell line of purify species New Zealand mice oral cavaty and palatal surface parts
  • Establishing and application of scale cancer cell line of purify species New Zealand mice oral cavaty and palatal surface parts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Establishment of pure New Zealand white rabbit oral and maxillofacial squamous cell carcinoma cell line

[0049] (a), material

[0050] 1. Experimental animals:

[0051] Purebred New Zealand white rabbits were purchased from Shanghai Chenhang Experimental Animal Breeding Factory, weighing 2-3 kg, male or female, and were raised in a clean animal room.

[0052] Nude mice were purchased from Shanghai Experimental Animal Center. The age of the mice was 4-5 weeks. They were both male and female. The experiments and feeding were carried out in an ultra-clean laminar flow frame under SPF conditions, and the sterilized water and feed were freely ingested by the animals.

[0053] 2. Tumor source:

[0054] Firstly, carcinogenic agent (DMBA) and oncovirus (rabbit papilloma virus) were used to induce oral and maxillofacial squamous cell carcinoma in purebred New Zealand white rabbits, and then the tumor tissue was surgically obtained from the animal with oral and maxillofacial s...

Embodiment 2

[0070] Biological characteristics of pure New Zealand white rabbit maxillofacial squamous cell carcinoma cell line

[0071] In this example, the biological characteristics of the purebred New Zealand white rabbit maxillofacial squamous cell carcinoma cell line with the deposit number CCTCC NO: C200504 were detected by conventional methods. Methods as below:

[0072] (a) Experimental method

[0073] 1. Morphology:

[0074] (1) General shape:

[0075] The surface morphology and activity of the transplanted tumor were observed and dissected. When the rabbit bearing the transplanted tumor was dying, the autopsy was performed. After the cultured cells were subcultured for 2-3 days, the growth morphology of the population cells was observed.

[0076] (2) light mirror:

[0077] Routine histopathological examinations were performed on induced tumors, transplanted tumors and transplanted tumors of each generation. The morphological structure and growth characteristics of cultured...

Embodiment 3

[0112] Establishment of a New Zealand White Rabbit Model of Oral and Maxillofacial Squamous Cell Carcinoma Using a Purebred New Zealand White Rabbit Maxillofacial Squamous Cell Cell Line

[0113] The pure New Zealand white rabbit maxillofacial squamous cell line CCTCC C200504 obtained in Example 1 was digested with digestive solution (0.25% trypsin, 0.02% EDTA, pH7.3), collected and counted, and counted as 1×10 8 or 1×10 9 The number of cells inoculated under the oral mucosa of 4 New Zealand white rabbits aged 2-3 months.

[0114] Between 24 and 36 days after inoculation, tumors appeared in the oral cavity of four New Zealand white rabbits. Carry out pathological detection with the same method of embodiment 2, confirm to induce the poorly differentiated maxillofacial squamous cell carcinoma on animal model [5] .

[0115] Culture preservation

[0116] The pure New Zealand white rabbit maxillofacial squamous cell carcinoma cell line of the present invention was preserved in ...

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Abstract

This invention supplies a pure oral and maxillofacial squamous-cell carcinoma cell system of the New Zealand rabbit and its construction method or application. The property of the talked maxillofacial squamous-cell carcinoma cell system is stable, and it can stably multiply many generations. It is suitable to construct model of the maxillofacial squamous-cell carcinoma cell system animal, and it is the ideal cell system of base and clinic early application of the maxillofacial squamous-cell carcinoma cell.

Description

technical field [0001] The invention relates to the field of biology, and more specifically relates to a pure New Zealand white rabbit oral and maxillofacial squamous cell line, its establishment method and application. Background technique [0002] The occurrence and development of various human diseases are very complicated, and in-depth exploration of the pathogenesis and therapeutic mechanism of various diseases cannot be carried out on patients. However, it is possible to follow the Animal Protection Law, conduct research on various diseases and life phenomena through animals, and then deduce it to human beings to explore the mysteries of human life and the laws of the occurrence and development of human diseases, so as to control the occurrence and development of human diseases, cure diseases and Reduce the suffering of human beings, improve the quality of life, and prolong the life of human beings. [0003] For a long time, people have found that it is difficult to p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
Inventor 郭伟金武龙周晓健任国欣张志愿陈万涛邱蔚六
Owner 郭伟
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