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Human pancreatic cancer cell line and its application

A technology for pancreatic cancer cells and pancreatic cancer, applied in the field of cell lines, can solve the problems of low biodiversity and large differences in biological properties of pancreatic cancer, and achieve the effect of stable properties

Active Publication Date: 2011-11-23
开拓者医学研究(上海)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the existing human pancreatic cancer cell lines have low biodiversity, which is relatively different from the clinical biological characteristics of pancreatic cancer. Big deficiencies, providing a new human pancreatic cancer cell line and its use

Method used

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  • Human pancreatic cancer cell line and its application
  • Human pancreatic cancer cell line and its application
  • Human pancreatic cancer cell line and its application

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Experimental program
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Effect test

Embodiment 1

[0036] The preparation of embodiment 1PAXC-003 cell

[0037] Nude mice: 5 nude mice, female, weighing 16.0±1.0 g, aged 5 weeks, raised in SPF environment. Nude mice were provided by Shanghai Slack Experimental Animal Technology Co., Ltd.

[0038] Fresh clinical pancreatic cancer resection specimens (male, 62 years old, primary pancreatic cancer, pathological diagnosis: moderately differentiated adenocarcinoma (uncinate process of pancreas)) were obtained from Shanghai Changhai Hospital, and immediately immersed in pre-cooled sterile HBSS buffer solution (containing 500 U / ml penicillin G, 500 μg / ml streptomycin sulfate and 1.25 μg / ml amphotericin B). In the biosafety cabinet, wash the specimen with fresh sterile HBSS buffer solution, cut into small pieces of 20-50 mg, and puncture and inoculate subcutaneously in the axillary back of nude mice. After inoculation, the animals were in good health and behaved normally. 40 days after inoculation, small tumor nodules were found un...

Embodiment 2

[0042] Biological characteristics and application of embodiment 2PAXC-003 cells

[0043] In the present invention, RPMI 1640 culture solution containing fetal bovine serum and insulin is used to cultivate PAXC-003 cells, so that the PAXC-003 cells can grow long-term in vitro and pass stably. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. Through experimental observation and verification, PAXC-003 cells grown in vitro have a typical epithelial-like morphology, lose contact growth inhibition, and show malignant growth. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors. The PAXC-003 cells can form tumors in nude mice and have tumorigenicity. The correspon...

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Abstract

The invention provides a human pancreatic cancer cell line and its preparation method and application. The human pancreatic cancer cell line is preserved in the China Center for Type Culture Collection and the preservation number is CCTCC C201007. The human pancreatic cancer cell line can be utilized for producing human pancreas cancer in mammals, preparing human pancreas cancer models and screening candidate drugs for treating human pancreas cancer. The human pancreatic cancer cell line has stable characters and is subcultured multiple times. Characters are stably maintained in 20th to 50th generation obtained by subculture in vitro. The human pancreatic cancer cell line has clinical biological characters of human pancreas cancer and provides an experimental material for pancreas cancer research, wherein biological characteristics of the experimental material are close to clinical tumor biological characteristics. Compared with a subcultured parent tumor in a nude mouse, the human pancreatic cancer cell line can be utilized for analysis of correlation of tolerance and susceptibility to drugs in vivo or vitro thus can establish two associated anti-pancreatic cancer drug screening platforms in vitro and in vivo.

Description

technical field [0001] The invention belongs to the field of cell lines, in particular to a human pancreatic cancer cell line and application thereof. Background technique [0002] Pancreatic cancer is a highly malignant disease. Although the relative incidence rate is generally 9-10 / 100,000, it is still the disease with the highest tumor-related mortality in Western countries; the incidence rate in my country is also increasing year by year. trend. Pancreatic cancer is not easy to detect at an early stage. No specific clinical manifestations and tumor markers have been found so far, and imaging features are not typical. Moreover, due to the anatomical and biological characteristics of the pancreas, it is easy to invade surrounding organs and metastasize. At that time, the disease is already in the advanced stage and has metastasized to the liver, lung, spine, kidney or adrenal gland and other organs, so only exploratory or palliative surgery can be performed, but the long-t...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027A61K49/00C12R1/91
Inventor 闻丹忆秦宵然胡刚欧阳可栋朱明华谢付波
Owner 开拓者医学研究(上海)有限公司
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