Rabbit portal vein thrombosis model

Abstract

The invention relates to model preparation, particularly to a rabbit portal vein thrombosis model which establishes a rabbit portal vein thrombosis model by a portal vein ligation catheterization method. The experimental animal adopts a New Zealand white rabbit; the experimental process comprises the steps of preoperative preparation: a preoperative experimental rabbit fasts all night and freely drinks water; anaesthetization; operation method: make the preoperative preparations including shaving feathers at the operating area after anaesthetization, disinfection and the like, enter the belly layer by layer, separate a portal vein, insert a 2Fr internal jugular vein tube by a catheterization method, ligate the portal vein using the tube as the inner support, suture and fix, lead to outside of body, and then conducting firm fixing. The model of the invention causes partial blood stasis, annular oppressed damage of blood vessel endothelium by ligating the portal vein, simultaneously carries out tube stimulation, starts intrinsic and extrinsic coagulation processes, increases the incidence of thromboembolism, and provides passages for the later partially collecting blood specimen, dynamic venogram, partial injection and the like; the first PVT occurrence time is about 30 min after the model is found via the portal vein radiography, and the model formation rate is higher than 75%.

Description

technical field [0001] The invention relates to model preparation, in particular to a rabbit portal vein thrombosis model. Background technique [0002] Portal vein thrombosis (portal vein thrombosis, PVT) refers to the thrombus that occurs in the main portal vein, superior mesenteric vein, inferior mesenteric vein or splenic vein. It is one of the serious complications after splenectomy (or combined vascular devascularization)[1]. Okuda reported the autopsy results of 247,728 patients from the Japanese Pathological Association, the incidence of portal vein thrombosis was 0.55%, the incidence of portal vein thrombosis in patients with liver cirrhosis was 6.59%, and the incidence of PVT after splenectomy was 37.5% to 43.5% [2]. Once PVT forms, it can lead to hepatic hypoperfusion and aggravated gastrointestinal congestion, and acute mesenteric venous thrombosis can lead to intestinal necrosis; extensive portal thrombosis will seriously affect the success rate of later liver ...

AI Technical Summary

Problems solved by technology

[0003] CN101961465A (2011-2-2) discloses a traditional Chinese medicine for treating portal vein thrombosis, but the traditional Chinese medicine needs to be taken for a long time and has little effect on preventing portal vein thrombosis

According to comprehensive literature reports, the number of rabbit venous thrombosis models is far less than that of rat venous thrombosis models, and the production of rabbit portal vein thrombosis models is even rarer, which may be related to the complicated production methods, high costs, and difficult management after modeling.

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