940 results about "In vitro diagnostic" patented technology

The invention relates to a mouse anti-PBP2a hybridoma cell strain, a monoclonal antibody and application of the mouse anti-PBP2a hybridoma cell strain, staphylococcus aureus PBP2a protein is extracted as an antigen, after the high-purity antigen is obtained, a good immunoreaction is aroused in a mouse body, then a hybridoma technology is adopted, two cell strains with high affinity and specificity are screened, and the corresponding antibody is prepared; the two antibodies are used as a gold-labeled antibody and a coating antibody in a colloidal gold immunoassay kit, and the kit can be used for detecting PBP2a protein in methicillin-resistant staphylococcus aureus and can be used for developing related in-vitro diagnostic reagents.

The invention belongs to the technical field of immunology and in vitro diagnosis, and particularly relates to a rabbit-derived recombinant monoclonal antibody for specific recognition of type II grass carp reovirus VP4 protein, an eukaryotic expression method and application. According to the invention, a target gene S6 is transfected to an HEK293 cell for expression and purification, a New Zealand white rabbit is immunized in cooperation with a Freund's adjuvant, and the rabbit-derived recombinant monoclonal antibody capable of specifically recognizing the GCRV-II VP4 protein is obtained by using ELISA, single B cell screening and eukaryotic expression technologies. The antibody has strong specificity, provides support for further establishment of a specific II-type grass carp reovirus diagnosis technology, and has great application value in development of GCRV-II related scientific research.

The invention discloses an in-vitro diagnosis liquid level detection method based on a 60GHz on-chip antenna millimeter wave radar, and relates to the technical field of liquid level detection in the IVD field, and the method comprises the steps: receiving a reflection signal through a sensor, and carrying out the preprocessing; extracting liquid level position data from the reflected signal through a machine learning algorithm; a temperature compensation and calibration mechanism is used for monitoring the temperature in real time and compensating and calibrating a measurement result; a friendly user interface is constructed and developed to feed back the system operation state in real time. The device can be accurate to a micron level, can accurately detect the liquid level height change in a micro cup or a reagent bottle, provides an extremely accurate reference for reagent addition, is greatly enhanced in reliability, is not influenced by factors such as liquid color, transparency, container material and shape, and can be used in a complex and changeable laboratory environment. The device can work stably and accurately under the conditions of temperature, humidity, electromagnetic interference and the like, and effectively avoids measurement errors caused by interference of external factors.

The invention belongs to the technical field of immunology and in vitro diagnosis, and relates to a monoclonal antibody aiming at bovine nodular skin disease virus (LSDV) L1R protein. According to the invention, the LSDV recombinant L1R protein with deletion of a transmembrane region (182-204aa) is obtained through expression; after a mouse is immunized by the protein, a monoclonal antibody 8D5 which is good in reactivity and specificity and aims at the LSDV L1R protein is screened out; heavy chain and light chain variable region sequences for coding the monoclonal antibody 8D5 are obtained; the monoclonal antibody 8D5 can be subjected to specific reaction with LSDV L1R protein, the recombinant protein L1R can be specifically recognized by bovine LSDV positive serum, meanwhile, the monoclonal antibody 8D5 can be used for establishing an LSDV ELISA antibody detection method, and a foundation is laid for LSDV diagnostic technology and vaccine research and development.

The invention provides a multi-channel one-core multi-detection integrated detection method, which is applied to the technical field of in-vitro diagnosis and comprises the following steps: acquiring time sequence original signals of blood routine, liver function and kidney function channels; obtaining a baseline stable primary signal through cross-channel baseline trend coupling and adaptive correction; adopting a machine learning model to identify interference components and performing component offset to obtain a decoupling signal; mapping the decoupling signal into a physiological parameter value and carrying out rationality test; when the low credible parameter exists, a rechecking mechanism is triggered; performing weighted fusion on the basis of the rationality test result and the recheck signal to obtain a comprehensive parameter set; and finally generating a comprehensive detection report containing the credibility score. Cooperative processing and fusion analysis of multi-channel detection signals are realized, and the accuracy and reliability of a detection result are remarkably improved through interference identification and a dynamic recheck mechanism.

The invention relates to the technical field of in-vitro diagnostic reagent chemiluminescence detection, in particular to a Christmas liquefied carbohydrate chain antigen chemiluminescence detection kit and a preparation method thereof. The chemiluminescence detection kit comprises the following components: a KL-6 calibrator, an amino magnetic bead coupled KL-6 monoclonal antibody, an alkaline phosphatase labeled KL-6 monoclonal antibody, an AMPPD rapid chemiluminescence substrate solution and a cleaning solution, and the chemiluminescence detection kit prepared from the components is simple in detection process, easy to operate, high in automation degree and high in detection sensitivity. The kit is high in sensitivity, good in specificity and good in stability, a result can be automatically obtained within 12.5 min, the requirement for clinical auxiliary diagnosis of interstitial lung diseases can be met, and the problem that in the prior art, a chemiluminescence detection kit prepared by adopting an acridinium ester magnetic particle chemiluminescence immunoassay method or a biotin-streptavidin detection system is poor in specificity and high in sensitivity is solved. The technical problems of low marking efficiency, high manufacturing cost and poor detection result reliability in the prior art are solved.

The invention relates to the technical field of in-vitro diagnosis automation equipment, and discloses a reaction container feeding device and diagnosis equipment, and the reaction container feeding device comprises a hopper, a partition plate, a driving assembly, a material pushing part and a feeding assembly; a feeding hole is formed in the top of the hopper, and an opening is formed in the bottom; the partition plate is fixedly arranged in the hopper and divides the opening into a first sub-opening and a second sub-opening; the driving assembly is arranged at the lower end of the hopper; the material pushing part and the feeding assembly are connected with the driving assembly, the driving assembly drives the material pushing part to move up and down and penetrate through the first sub-opening so as to jack up the reaction container, and the driving assembly drives the feeding assembly to move up and down and penetrate through the second sub-opening so as to receive the reaction container jacked up by the material pushing part. The driving assembly is adopted to drive the material pushing piece to jack up the reaction container to the feeding assembly, meanwhile, the feeding assembly is driven to move upwards to receive the reaction container, the feeding structure of the reaction container is simplified, the occupied space is small, and the reliability is high.

The invention belongs to the technical field of in-vitro diagnosis, and discloses a microbiological detection kit and a detection method. The kit comprises an elisa plate coated with a captured antibody and an HRP labeled antibody working solution, the capture antibody is a monoclonal antibody LG3A-1 specifically combined with a human giardia antigen, the heavy chain amino acid sequence of the capture antibody is as shown in SEQ ID NO.1, and the light chain amino acid sequence of the capture antibody is as shown in SEQ ID NO.2; the HRP labeled antibody is a monoclonal antibody FLSP4 specifically combined with human giardia antigen, the heavy chain amino acid sequence of the monoclonal antibody FLSP4 is as shown in SEQ ID NO.3, and the light chain amino acid sequence of the monoclonal antibody FLSP4 is as shown in SEQ ID NO.4. The microbiological detection kit provided by the invention can be used for detecting the content of giardia in human excrement, is high in specificity, high in sensitivity, good in stability and simple to operate, and has a very good application prospect.

The invention relates to the technical field of in-vitro diagnosis automation equipment, in particular to a pipe cap limiting block, a cap opening device and a using method of the cap opening device. And the number of the tube cover limiting blocks is multiple, and each tube cover limiting block comprises a limiting block bottom face connected with the tube cover bottom face in an abutting mode. And the plurality of test tubes are limited and fixed through the plurality of tube cover limiting blocks. Furthermore, the tube cover can be moved to the tube cover limiting block through one clamping jaw, and then the next tube cover is transferred through the clamping jaw, so that the opening and closing actions of the tube covers of a plurality of test tubes are realized. According to the structure, cover opening and closing actions are executed on a plurality of specific test tubes through one set of equipment, and the test tube cover opening and closing efficiency is high.

A computer implemented method for designing a complex part of an in-vitro diagnostic instrument is disclosed. The method includes retrieving at least one target constraint, retrieving at least one set of input parameters, determining at least one design solution of the complex part by using at least one iterative algorithm considering the input parameters, comparing the candidate complex part to the target constraint, and rating the candidate complex part depending on the comparison.

The invention relates to the technical field of in-vitro diagnosis automation equipment, and discloses a feeding device cup arranging structure, a feeding device and diagnosis device.The feeding device cup arranging structure comprises a containing channel, a moving assembly, a base and a driving part; a feeding hole is formed in the top of the accommodating channel; the moving assembly is arranged in the containing channel, can move in the vertical direction of the containing channel and is used for bearing the reaction cup; the base is arranged at the lower end of the containing channel, the driving piece is arranged on the base, the moving assembly is connected with the driving piece through the connecting piece, and the driving piece is used for driving the moving assembly to move up and down along the containing channel. The moving assembly is arranged in the containing channel, the reaction cups collide with the inner wall of the containing channel in the falling process, under the action of gravity, the opening ends of the reaction cups are upwards arranged at the moving assembly, the cup clamping and cup transverse phenomena are avoided, and the reliability is high.

The invention provides a chemically modified tetanus toxin C fragment as well as preparation and application thereof. The method for chemically modifying the tetanus toxin C fragment comprises the following steps: 1) reacting the tetanus toxin C fragment with a reducing agent to reduce a disulfide bond in the tetanus toxin C fragment; and 2) carrying out a reaction on the reduced tetanus toxin C fragment and an alkylation reagent, and carrying out modification. The recombinant tetanus toxin C fragment prepared by the method disclosed by the invention not only meets the quality standard required by pharmacopeia, but also has the advantages of stable conformation, high yield, easiness in amplification and low cost, and has a wide application prospect in the preparation of human vaccines, veterinary vaccines and antibody drugs and the development process of in-vitro diagnostic reagents.

The invention provides a micro-fluidic chip and a preparation method and application thereof, and belongs to the field of in vitro diagnose.The micro-fluidic chip comprises aptamer magnetic beads, a reactor and multivalent DNA nanowire reaction liquid, the aptamer magnetic beads comprise aptamer magnetic beads labeled by biotinylated staphylococcus aureus aptamers and aptamer magnetic beads labeled by biotinylated escherichia coli aptamers; the aptamer magnetic beads comprise aptamer magnetic beads labeled by biotinylated staphylococcus aureus aptamers and aptamer magnetic beads labeled by biotinylated escherichia coli aptamers; the reactor comprises two sample micro-chambers, two magnetic suction chambers, two snakelike mixing channels, two detection chambers and a sample outlet; the multivalent DNA nanowire reaction solution is a mixed solution comprising a multivalent DNA nanowire, heme and Tris-HCl. The micro-fluidic chip provided by the invention has the advantages of high sensitivity, strong specificity, low cost and simplicity in operation.

The invention relates to the technical field of in-vitro diagnostic kits, and provides an apolipoprotein E4 detection kit and application thereof. The immunochromatography test paper comprises detection immunochromatography test paper, the detection immunochromatography test paper comprises a bottom plate, a sample pad, a combination pad, an NC membrane and a water absorption pad, the combination pad and the sample pad are sequentially arranged on one side of the NC membrane, and the water absorption pad is arranged on the other side of the NC membrane; the combination pad is marked with gold manganese oxide nanometer developing particles and an apolipoprotein APOE4 monoclonal antibody. According to the technical scheme, the problems of complicated operation, high cost, low detection sensitivity and weak specificity of apolipoprotein E4 detection in related technologies are solved.

The invention relates to the technical field of in-vitro diagnostic kits, in particular to carboxyl polystyrene microspheres, a preparation method and application of the carboxyl polystyrene microspheres in a kit for quantitatively detecting MxA. The preparation method of the carboxyl polystyrene microspheres comprises the steps that firstly, polystyrene microspheres with the surface rich in aldehyde groups are prepared through a soap-free emulsion polymerization method; then carrying out a Schiff alkali reaction with 2-aminoethyl methacrylate hydrochloride to carry out double bond modification on the surface of the microsphere, and meanwhile, improving the hydrophilicity of the microsphere by an ester group and an imino group; and finally, carrying out sulfydryl-double bond addition reaction on the polystyrene microspheres and mercaptosuccinic acid to generate the polystyrene microspheres rich in carboxyl on the surfaces. The carboxyl polystyrene microspheres prepared by the invention can improve the binding efficiency of latex and an MxA antibody, so that the detection sensitivity is improved, the purpose of high-sensitivity quantitative detection of MxA protein can be achieved, and the detection time is greatly shortened.

The invention discloses a colorimetric-fluorescent bimodal nanoprobe for detecting cMyBP-C and a micro-fluidic chip, and belongs to the technical field of biomedical detection and in-vitro diagnosis. The colorimetric-fluorescent bimodal nanoprobe which takes UCNPs as a core and MOF as a shell and is loaded with horse radish peroxidase and a labeled antibody is prepared by providing a layer-by-layer self-assembly technology; the micro-fluidic chip is prepared according to the probe, the micro-fluidic chip can realize cMyBP-C colorimetric and fluorescence signal bimodal detection, and the technical problems of limited sensitivity, susceptibility to background interference, limited linear range, photobleaching phenomenon, signal instability and the like in single signal detection are solved.

The invention belongs to the technical field of in-vitro diagnosis, and particularly relates to a detection kit and a detection method for a pretumor marker. The kit comprises an elisa plate coated with a captured antibody, an HRP labeled antibody, an antigen standard substance, a washing solution, a developing solution and a stop solution, the capture antibody and the HRP labeled antibody are both prepared from a monoclonal antibody of the anti-SLC31A1 protein. According to the detection method of the kit for detecting the pretumor marker, the SLC31A1 protein content in human serum is detected through the kit, the specificity is high, a large batch of samples can be rapidly detected in a high-throughput mode, the cancer risk of a to-be-detected crowd can be effectively judged, and therefore relevant patients can find and treat the pretumor marker in advance, treatment prognosis is improved, and the death rate is reduced.

The invention relates to a biotechnology, and particularly discloses a soluble fibrous protein (SF) antibody and an application of the soluble fibrous protein antibody. According to the present invention, the single B cell antibody preparation technology and the flow cytometry sorting method are firstly applied to the blood coagulation in vitro diagnosis fibrinolysis system detection antibody development, and the mammalian cell expression system (293F) is adopted to successfully express and obtain the three strains of the SF monoclonal antibodies (the antibody numbers are SF-1, SF-2 and SF-3) with the biological activity; the three antibodies have specific reactions with SF, have no cross reactions with primary fibrinolytic substances (X, Y and D), can be applied to the fields of SF indirect ELISA and Western blotting detection and screening, make up the vacancy of domestic SF antibodies in the market, and realize fibrinolytic system detection integrity.

The invention belongs to the field of in-vitro diagnosis and detection, and particularly relates to a primer pair, a probe, a kit and a detection method for norovirus detection. The norovirus detection primer pair provided by the invention comprises a primer pair for detecting a norovirus GI type, a primer pair for detecting a norovirus GII type and a primer pair for detecting a norovirus GIV type, and specific primers and probes are designed on the basis of a norovirus GI / GII / GIV type gene conserved region; and a plurality of pairs of primers or probes are introduced, and degenerate primers and degenerate probes are introduced, so that the existing norovirus subtypes are better matched, the detection coverage rate is increased, and the omission ratio is reduced.

The application belongs to the technical field of immunology and in vitro diagnosis, and particularly relates to a rabbit-derived recombinant monoclonal antibody specifically recognizing type II grass carp reovirus VP4 protein, a eukaryotic expression method and application. The S6 gene in GCRV-II encodes VP4 protein, the application transfects the target gene S6 into HEK293 cells for expression and purification, cooperates with Freund's adjuvant to immunize New Zealand white rabbits, and uses ELISA, single B cell screening and eukaryotic expression technology to obtain a rabbit-derived recombinant monoclonal antibody specifically recognizing GCRV-II VP4 protein. The antibody has strong specificity, provides support for further establishment of specific type II grass carp reovirus diagnosis technology, and has great application value for development of GCRV-II related scientific research.

The invention is suitable for the field of in vitro diagnostic equipment, and discloses a sample analyzer and a control method of an ultrasonic transducer. A control device of the sample analyzer is configured to control an ultrasonic transducer to execute frequency locking operation in a uniform mixing period of a target liquid, and before a preset condition is not met, if the driving frequency of the ultrasonic transducer is adjusted to exceed a first preset frequency range, the target liquid is subjected to frequency locking operation; if yes, judging whether the accumulated parameter of the frequency locking operation in the single mixing period is greater than or equal to a first preset value or whether the remaining parameter capable of performing the frequency locking operation in the single mixing period is smaller than a second preset value or whether the remaining duration in the single mixing period is smaller than or equal to a third preset value, and if yes, judging that the frequency locking fails; and if not, restarting the ultrasonic transducer to carry out frequency locking operation again. The frequency locking success rate is increased through multiple times of frequency locking in the uniform mixing period, and unnecessary waste of samples and reagents is reduced.

To provide a stainproof member capable of exhibiting both low protein adsorption performance and service durability.SOLUTION: Provided is a stainproof member with a membrane disposed on a substrate. The membrane has a condensate of a hydrolysate of a silane coupling agent or a condensate of a phosphonic acid derivative. The membrane has no halogen atoms, and in water at pH=7, a zeta potential of the membrane is -10.0 mV or more and 10.0 mV or less. When an amount of C atoms (atomic%) / an amount of atoms of an element X that is most abundant on the surface of the substrate (atomic%) is defined as A in an X-ray photoelectron spectroscopy analysis (ESCA measurement) on the substrate, and an amount of C atoms (atomic%) / an amount of atoms of the element X (atomic%) is defined as B in the X-ray photoelectron spectroscopy analysis (ESCA measurement) on the membrane of the substrate is defined as B, the value of B / A is 2.0 or more and 5.0 or less.SELECTED DRAWING: None

The invention relates to the technical field of reagent cold-chain transportation monitoring, and particularly discloses a dynamic monitoring and adjusting system for cold-chain transportation of in-vitro diagnostic reagents, which is used for solving the problem that the temperatures of some regions cannot reach or keep a set value in time because local hot spots or cold regions are formed due to non-uniform stacking of the existing in-vitro diagnostic reagents. According to the method, the global temperature data and the reference three-dimensional temperature data in the warehouse are acquired, the first temperature abnormal fluctuation value is calculated, the abnormal deviation between the local temperature data and the global data is automatically identified, the consistency of the temperature data is ensured, the temperature gradient is quantitatively calculated from the global and local angles, and the accuracy of the temperature gradient is improved. Temperature distribution characteristics in the warehouse can be captured in detail, temperature gradient difference values are calculated by comparing different data sources, a reasonable threshold value is set, and automatic early warning is achieved, so that the stability of a temperature control environment is guaranteed, and the quality of an in-vitro diagnostic reagent is protected.

The invention discloses a magnetic porous microsphere for in-vitro diagnosis and a preparation method of the magnetic porous microsphere, and belongs to the technical field of preparation of micro-nano materials. According to the invention, styrene is used as a polymerization monomer, and a dispersion polymerization method is adopted to prepare monodisperse polystyrene microspheres; ferric chloride and ferrous sulfate are selected as precursors, magnetic nanoparticles are synthesized through a coprecipitation method under the alkaline condition, the initial loading capacity is changed, the magnetic nanoparticles are loaded on the surfaces and in pore channels of polymer porous microspheres through a multi-step seed growth method, and the magnetic nanoparticles are prepared. The composite magnetic polystyrene microspheres with high magnetic content, good magnetic responsiveness and superparamagnetism are synthesized; finally, functional groups are introduced to the surfaces of the magnetic spheres by monomers containing the functional groups through a monomer swelling polymerization method, and the prepared magnetic porous microspheres have the advantages of uniform particle size, small coefficient of variation and large specific surface area, and can be better combined with antigens and antibodies, so that in-vitro diagnostic function detection such as nucleic acid extraction and chemiluminescence is carried out.

The invention relates to the technical field of automatic equipment for in-vitro diagnosis, in particular to a method for cleaning a reaction cup, a mode selection method and a chemiluminescence immunoassay analyzer. The method for cleaning the reaction cup comprises the following steps that the first gripper is driven to move on the gripper movement axis in the linear direction, the reaction cup in a reaction cup hole in a first target is grabbed, and the cup throwing action is completed; meanwhile, the second gripper is driven to move on the gripper movement axis in the linear direction, the reaction cup in the reaction cup hole in the second target is grabbed, and the cup throwing action is completed. The reaction cups in the reaction cup holes can be cleaned through linear motion of the grippers, and the multiple grippers work separately. The problems that in the prior art, cup grabbing and cup throwing actions can be achieved only by moving cup grabbing hands for a long distance, avoiding control logic is lacked between the cup grabbing hands, position interference is prone to occurring, and the cup cleaning efficiency of an analytical instrument is affected are effectively solved.

The invention relates to the technical field of in-vitro diagnosis, in particular to a new bunyavirus antibody and application thereof. The invention provides a novel bunyavirus antibody and application thereof. The new bunyavirus antibody obtained by screening can be specifically combined with different mutation types of new bunyavirus, is high in sensitivity and strong in specificity, and has a very good market application prospect. The detection sensitivity of the SFTSV is measured by using a double-antibody sandwich method, and the result shows that the antibody detection sensitivity of the novel bunyavirus provided by the invention is as low as 0.5 ng / mL, and the novel bunyavirus can be used for early diagnosis and detection of the SFTSV.

The invention relates to the field of monoclonal antibodies, in particular to a von Willebrand factor cleavage protease (ADAMTS13) monoclonal antibody as well as a preparation method and application thereof. The ADAMTS13 monoclonal antibody provided by the invention is high in sensitivity and strong in affinity and can effectively inhibit ADAMTS13 enzyme activity, in addition, the invention also develops an in-vitro diagnosis detection kit applying the antibody, and the kit has the advantages of wide detection range, few interfering substances, short detection time and good stability and repeatability. And a new thought is provided for treatment and research of diseases.

The invention relates to the technical field of biochemical immune in-vitro diagnostic reagents, in particular to a method for eliminating lipid interference, an immunoturbidimetric kit and application of the immunoturbidimetric kit. Surfactants are added into the kit, that is, one or more compound surfactants with low cost and good lipid solubility are added into a reagent I; therefore, the problem of lipid interference can be effectively solved, the lipid interference can be eliminated by adopting a low-cost, high-accuracy and simple-to-operate method for eliminating lipid interference, and the problems that when an existing kit uses enzyme substances to eliminate lipid interference, the reagent cost is greatly increased due to addition of the enzyme substances, and the detection sensitivity is high are solved. The technical problem that the cost pressure of a conventional biochemical immune reagent is increased is solved, and meanwhile, the method can be widely used in cooperation with various biochemical analyzers and has wider universality, so that the method can be widely applied to the lipid blood sample diagnosis process.

The invention discloses an IV-type collagen detection kit and a preparation method thereof, and relates to the technical field of in vitro diagnos.The kit comprises a reagent R1 and a reagent R2, and the volume ratio of the reagent R1 to the reagent R2 is 3: 1; through an elaborately designed reagent formula, especially the accurate proportion of all components in the reagent R1, such as ammonium chloride, sodium azide and Brij-35, the detection sensitivity and accuracy are improved, the stability and repeatability of the kit are also ensured, and particularly, the natural plant extract astragalus polysaccharide and the protease K inhibitor are introduced into the reagent R1 as key components, so that the detection sensitivity and accuracy are improved, and the detection sensitivity and repeatability are improved. According to the present invention, the unique preparation method and the activity promotion effect on the IV-type collagen hydrolase significantly enhance the biological activity of the kit, such that the detection result is reliable, and the two latex particles coated with the paired mouse monoclonal antibodies adopted in the reagent R2 have high specificity and affinity, such that the kit can be used for the detection of the IV-type collagen hydrolase. And the detection accuracy and sensitivity are further improved.

The invention relates to the field of in-vitro diagnostic equipment, and provides a pipetting system and an immunoassay analyzer, the pipetting system comprises a pipetting vertical driving assembly and a pipetting crevasse assembly, the pipetting vertical driving assembly comprises a first driving structure, a first ball screw structure, a first compression spring, a spring limiting sleeve, a nut adjusting mounting block, a guide adjusting pin, a first separation blade, a first sensor and a first mounting seat, and the first ball screw structure comprises a first screw rod, a first screw rod nut and a ball; the pipetting crevasse assembly comprises an injection mounting frame and an injection pump, and a pump head of the injection pump is used for loading a disposable TIP head. When the disposable TIP head is loaded, the loading force pressed on the disposable TIP head is buffered by the first compression spring, and the precise control of the loading force is completed through the cooperation of the first separation blade and the first sensor, so that the technical problem that in the prior art, the loading is unsuccessful due to the too small loading force or the disposable TIP head is crushed due to the too large loading force is solved.