Dilution being capable of maintaining high stability of enzyme marker solution

A technology of high stability and diluent, which is applied in the field of diluent of new enzyme markers, can solve the problems of short shelf life, increased user workload, inconvenient experiment development, etc., and achieve the effect of improving protection and maintaining stability

Active Publication Date: 2009-10-21
福建省洪诚生物药业有限公司
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AI Technical Summary

Problems solved by technology

[0016] At present, many in vitro diagnostic kits often use concentrated and refrigerated enzyme markers. The concentrated enzyme markers are quite stable. They are often stored at -10°C by adding 30-40% glycerol, but this requires users to reconstitute or dilute them. Prepare your own working solution, which not only increases the workload of the user, but may also cause new operational errors; at the same time, the enzyme marker after simple dilution can only be prepared and used immediately, and the shelf life is short, which is very difficult for research. The work brings a lot of inconvenience; due to the inevitable need to repeatedly freeze and thaw the concentrated enzyme markers during use and storage, in order to solve the problem of enzyme marker stability and i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Diluents for enzyme-conjugated monoclonal antibodies:

[0051] Buffer system: use 0.01~0.1M PH7.2PBS (phosphate saline buffer);

[0052] Protein matrix solution: 5-50% calf serum;

[0053] Amino acid and sugar solution: compound amino acids, mainly: 0.05-0.3M glycine, lysine, arginine; 0.1-2% xylose, glucosamine, sucrose;

[0054] Special effect additives: 0.1-2% plant extract, 0.1-10% rat serum, 0.1-2% potassium gluconate, 0.05-0.1% sodium octanoate; 0.05-0.1% Tween-20;

[0055] Preservative: 0.05-0.1% PROCLIN300 diagnostic reagent preservative;

[0056] The dilution method is as follows:

[0057] Add 0.05% PROCLIN300 diagnostic reagent preservative and 1% xylose, 1% glucosamine and 1% sucrose, and 0.1M glycine, 0.1M lysine and 0.1M essence to 0.05M PH7.2PBS Add 1% aloe vera extract, 5% mouse serum, 1% potassium gluconate, 0.05% sodium octanoate and 0.05% Tween-20; finally add 20% calf serum, mix well That's it. The aloe extract can be prepared from commercially ...

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Abstract

The invention relates to a diluent being capable of maintaining the high stability of enzyme marker solution and also relates to a method for preparing the diluent and applications thereof. The diluent comprises buffer solution, protein matrix solution, amino acid solution, sugar solution, a specific additive and a preservative. The diluent is a novel enzyme marker solution developed based on the characteristics of antibody protein and enzyme protein. With the adoption of the dilution, the stability of enzyme markers in storage and application can be effectively maintained. Tests show that the enzyme marker diluent can be stored at temperature of 37 DEG C for more than one week to fully meet the requirements for the quality of in-vitro diagnostic reagent kits. The diluent can be directly applied to the preparation of the in-vitro diagnostic reagent kits and meet the increasingly growing market demand for the in-vitro diagnostic reagent kits.

Description

technical field [0001] The invention relates to a novel diluent of an enzyme marker. The diluent is used to prepare an enzyme marker solution, which can effectively maintain the stability of the enzyme marker during storage and use, and fully meet the quality requirements of an in vitro diagnostic kit. At the same time, it also relates to the preparation method and application of the diluent of the enzyme marker. Background technique [0002] The immunoassay technique using labeled antigen or antibody is an in vitro ultra-trace analysis technique widely used in modern medicine, biology and other fields. It can accurately measure 10-9-10-15g trace substances, thus solving the problem that other methods cannot The problems solved have greatly promoted the rapid development of various research work and clinical tests in the fields of biology and medicine, and made extraordinary contributions to human scientific progress and health. [0003] Since the invention of radioimmunoas...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/576G01N33/577
Inventor 高淑舫
Owner 福建省洪诚生物药业有限公司
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