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Double detection line SAA (Serum amyloid A protein) immunofluorescence chromatography quantitative detection reagent and preparation method thereof

A technology of immunofluorescence and quantitative detection, which is applied in the field of double detection line SAA immunofluorescence chromatography quantitative detection reagent and its preparation, and in the field of immunofluorescence chromatography quantitative detection reagent, which can solve the problems of poor repeatability of measured values ​​and large fluctuation of C value , to achieve the effect of improving accuracy, stabilizing fluorescence value and reducing error

Inactive Publication Date: 2016-07-20
GUANGZHOU WEIMI BIOLOGICAL SCI & TECH
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Problems solved by technology

[0011] Chinese patent 201510583004.1 discloses a CRP / SAA quantitative joint detection immunofluorescence chromatography test paper and its preparation method, and Chinese patent 201510014077.9 discloses a PCT / SAA combined rapid detection test strip and its preparation method, but for the rapid detection of SAA In immunochromatography, colloidal gold immunochromatography and fluorescence immunochromatography are generally coated with an anti-IgG monoclonal antibody or anti-IgG polyclonal antibody on the coating membrane 4 when setting the quality control line C. It is used to capture colloidal gold or fluorescent microsphere-labeled monoclonal antibodies, so the C value fluctuates greatly due to the influence of the chromatography effect during the measurement process, and the effect is only whether the test strip test results Effective, in the quantitative detection experiment, the repeatability of the measured value is poor

Method used

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  • Double detection line SAA (Serum amyloid A protein) immunofluorescence chromatography quantitative detection reagent and preparation method thereof
  • Double detection line SAA (Serum amyloid A protein) immunofluorescence chromatography quantitative detection reagent and preparation method thereof
  • Double detection line SAA (Serum amyloid A protein) immunofluorescence chromatography quantitative detection reagent and preparation method thereof

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Embodiment 1

[0056] A kind of double detection line SAA immunofluorescence chromatography quantitative detection reagent provided by the invention, its structure is as follows figure 1 As shown, it consists of a PVC base plate 1, a sample pad 2, a bonding pad 3, a coating film 4, and an absorbent pad 5 in sequence. The same fluorescent microsphere-labeled anti-SAA monoclonal antibody 1 and chicken IgY were sprayed on the binding pad 3; 1 Position coated with anti-SAA mAb 2, T 2 The position is coated with the antigen SAA protein, and the position of the C line is coated with goat anti-chicken IgY. Preparation method of SAA fluorescent microsphere immunochromatography detection reagent

[0057] (1) Preparation method of sample pad

[0058] Soak a sample pad with a size of 20cm×30cm in the sample pad buffer, take it out after 30 minutes, dry it at room temperature for 16-18 hours, and cut it into a sample pad with a size of 1.6cm×30cm after drying.

[0059] The buffer formulation of the ...

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Abstract

The invention discloses a double detection line SAA (Serum amyloid A protein) immunofluorescence chromatography quantitative detection reagent and a preparation method thereof. The double detection line SAA immunofluorescence chromatography quantitative detection reagent can simultaneously promote sensitivity and detection scope and can be applied to clinical detection for SAA level in patients of acute inflammation and chronic inflammation. According to the technical key points, the reagent comprises a base plate, a combining cushion, a coating film and an absorbing cushion, wherein a sample cushion is connected with the base plate; anti-SAA monoclonal antibody 1 and chick IgY are sprayed on the combining cushion and are marked with a same fluorescent microsphere; a detection line T1, a detection line T2 and a quality control C line are arranged on the coating film; anti-SAA monoclonal antibody 2 is coated with the detection line T1; antigen SAA protein is coated with the detection line T2; goat-anti-chick IgY is coated with the quality control C line. The reagent belongs to the technical field of in-vitro diagnostic reagents.

Description

technical field [0001] The invention provides a quantitative detection reagent of immunofluorescence chromatography, specifically, a double detection line SAA immunofluorescence chromatography quantitative detection reagent and a preparation method thereof; belonging to the technical field of in vitro diagnostic reagents. Background technique [0002] Serum amyloid A (Serumamyloid Aprotein, SAA), is a sensitive marker of acute inflammation, normal human serum SAA value is 0-0.78mg / l, when the body is stimulated by infection, inflammation, injury, tumor, etc., the liver The cells secrete a large amount of SAA. With the development of the body's inflammatory response, the level of SAA in the serum can rapidly increase by about 1000 times within 5-6 hours, and the SAA can reach the peak within 8-12 hours, and the increase can reach 2000 times the normal value. , in clinical diagnosis, when the SAA content> 10mg / l, it can be used as a sign of acute inflammation. When the bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/543G01N33/533
CPCG01N33/6893G01N33/533G01N33/54313G01N33/558G01N33/577G01N2333/47G01N2800/7095
Inventor 汤永平李之华张晓丽潘秀华解巧丽
Owner GUANGZHOU WEIMI BIOLOGICAL SCI & TECH
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