Study and application of quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting trace multicomponent food additives quickly on site

A technology for molecularly imprinted microspheres and food additives, applied in measurement devices, fluorescence/phosphorescence, color/spectral property measurement, etc., can solve the problems of large amount of reagents, false positive results, low detection sensitivity, etc. Sensitivity, multi-component rapidity, and simple detection steps

A technology for molecularly imprinted microspheres and food additives, applied in measurement devices, fluorescence/phosphorescence, color/spectral property measurement, etc., can solve the problems of large amount of reagents, false positive results, low detection sensitivity, etc. Sensitivity, multi-component rapidity, and simple detection steps

CN102012358AInactive Publication Date: 2011-04-13UNIV OF JINAN
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  • Study and application of quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting trace multicomponent food additives quickly on site
  • Study and application of quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting trace multicomponent food additives quickly on site

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preparation example Construction

[0044] A method for preparing a quantum dot molecularly imprinted quartz fluorescent sensor for detecting trace amounts of food additives is characterized by comprising the following steps:

[0045] (1) Quantum dots with different particle sizes with high quantum yields are prepared, and purified respectively to prepare monodisperse solutions.

[0046] (2) Select functional monomers that can synthesize molecularly imprinted polymers with food additives;

[0047] (2) According to a certain molar ratio, the template molecule of the food additive is uniformly mixed with the quantum dots of the corresponding functional monomer, cross-linking agent and initiator with a specific particle size to make quantum dot molecularly imprinted microspheres;

[0048] (3) Design and manufacture a comb-shaped multi-branched quartz plate and a sealed quartz fluorescence excitation dish, in which a saturated fluorescent reagent for short-wave emission is housed;

[0049] (4) Using the layer-by-la...

Embodiment 1

[0067] Example 1 (colorants such as sunset yellow, erythrosine and carmine)

[0068] A method for preparing a quantum dot molecularly imprinted quartz fluorescent sensor for simultaneous detection of sunset yellow, erythrosine and carmine, comprising the following steps:

[0069] (1) Select the functional monomers phenyltrimethoxysilane (PTMOS) and 3-aminopropyltriethoxysilane (APTS) that can synthesize molecular imprinting with sunset yellow; select the function that can synthesize molecular imprinting with erythrosine Monomer 3-aminopropyltriethoxysilane (APTS) diphenyldiethoxysilane (DEOS); functional monomers octyltrimethoxysilane (OTES) and benzene selected for molecular imprinting synthesis with carmine Vinylethyltrimethoxysilane (SETS);

[0070] (2) Preparation of three kinds of CdSe quantum dot solutions with different particle sizes: in N 2 Under protection, dissolve Se powder in TOP to prepare Se precursor, dissolve S powder in TOP to prepare S precursor, and then ...

Embodiment 2

[0076] Example 2 (preservatives such as benzoic acid, sorbic acid and propylparaben)

[0077] A method for preparing a quantum dot molecularly imprinted quartz fluorescent sensor for simultaneous detection of benzoic acid, sorbic acid and propyl p-hydroxybenzoate, comprising the following steps:

[0078] (1) Select functional monomers phenyltrimethoxysilane (PTMOS) and 3-aminopropyltriethoxysilane (APTS) that can synthesize molecular imprinting with benzoic acid; select functional monomers that can synthesize molecular imprinting with sorbic acid Body 3-aminopropyltriethoxysilane (APTS) and propyltriethoxysilane (TEOPS); select the functional monomer methyltriethoxysilane ( MTEOS), phenyltriethoxysilane (PTEOS);

[0079] (2) Preparation of three kinds of CdTe quantum dot solutions with different particle sizes: in N 2 Under protection, dissolve Se powder in TOP to prepare Te precursor, dissolve S powder in TOP to prepare S precursor, and then combine with the prepared Cd(OA)...

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Abstract

The invention discloses a quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting multicomponent food additives simultaneously and a method for detecting the food additives by the quantum dot molecular imprinting microsphere quartz fluorescent sensor. A method for preparing a quantum dot molecular imprinting microsphere comb quartz plate comprises the following steps of: selecting functional monomers corresponding to the food additives; preparing quantum dots and preparing quantum dot molecular imprinting microspheres according to the literature; and modifying thesurfaces of different probes of the comb quartz plate with the imprinting microspheres of different food additives by utilizing layer-upon-layer accumulated surface modification technology. The method for detecting the trace multicomponent food additives simultaneously (which is shown as the figure) comprises the following steps of: immersing the modified quartz plate into simply-pulpified food solution, arranging the quartz plate on a sealing quartz vessel, and detecting the food additives in a sample. The quantum dot molecular imprinting microsphere quartz fluorescent sensor has high specificity and sensitivity, short detection time and low cost; and in a method for detecting pesticide residues by fluorescent light, the operation is simple and quick, and reactions and results are completed and recorded automatically by instruments.

Description

technical field [0001] The present invention relates to the technical field of on-site rapid high-throughput food additive detection, more specifically to the preparation of a quantum dot molecularly imprinted microsphere quartz fluorescent sensor for detecting trace food additives. The present invention also relates to the use of the quantum dot molecule Method for on-site detection of trace food additives in food, wine and beverage samples by imprinted microsphere quartz fluorescent sensor. Background technique [0002] Food additives mainly include antioxidants, colorants, color protectants, flavor enhancers, preservatives, sweeteners, emulsifiers, thickeners, etc. according to the different uses of the additives. These food additives, which are permitted by regulations and considered safe, do not guarantee absolute safety in fact, and may cause physical and health damage to some people under certain conditions. For example, aspartame is considered the ideal sweetener fo...

Claims

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Application Information

Patent Timeline
13 Apr 2011
Publication
CN102012358A
IPC
G01N21/01; G01N21/64; G01N21/31; C09K11/88; C09K11/06
Inventors
于京华; 葛磊