Polystyrene fluorescent nanometer particle as well as preparation method and applications thereof
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A fluorescent nanoparticle, polystyrene nanotechnology, applied in the direction of luminescent materials, chemical instruments and methods, instruments, etc.
Inactive Publication Date: 2013-06-26
SUZHOU SYM BIO LIFESCI CO LTD
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Harma H, Ullman EF etc. reported the application of organic polymer fluorescent nanoparticles in conventional heterogeneous immunoassay and homogeneous immunoassay (Ullman EF, Kirakossian H, Singh S, et al.Proc.Natl.Acad.Sci. USA.1994, 91, 5426-5430; Harma H, Soukka T and Lovgren T, Clinical Chemistry, 2001, 47, 561-568), but there is no report on the application of this type of fluorescent nanoparticles in immunochromatography
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Embodiment 1
[0102] Example 1: Preparation of polystyrene-acrylic acid nanoparticles by emulsion polymerization
[0103] Proceed as follows:
[0104] (1) Add 10ml of deionized water containing 0.4mmol SDS (sodium dodecylsulfonate) to a round bottom flask with a stirring bar, and protect with nitrogen gas;
[0105] (2) add 70mmol styrene (remove polymerization inhibitor) and 10mmol acrylic acid monomer (remove polymerization inhibitor) under stirring;
[0106] (3) Gradually increase the reaction temperature to 70° C., add 5 mmol of potassium persulfate (after recrystallization), and stir for 10 hours;
[0107] (4) reaction system is cooled to room temperature;
[0108] (5) Filter the product in a 0.45-micron syringe filter, centrifuge in an ultracentrifuge at 60,000 rpm for 50 minutes, dissolve the precipitate with 100 mM sodium bicarbonate buffer (pH 9.0), and store at 4°C.
[0109] The polystyrene-acrylic acid nanoparticles synthesized in this example have a particle size of 10-100 nm. ...
Embodiment 2
[0110] Example 2: Preparation of polystyrene-methacrylic acid nanoparticles by emulsion polymerization
[0111] Carry out according to the method for embodiment 1, but change the acrylic acid monomer (remove polymerization inhibitor) wherein into 3mmol methacrylic acid (remove polymerization inhibitor) monomer, change temperature of reaction into 68 ℃, stir reaction into 6 hours, With the rest unchanged, polystyrene-methacrylic acid nanoparticles were obtained.
Embodiment 3
[0112] Example 3: Preparation of polystyrene-acrylamide nanoparticles by emulsion polymerization
[0113] Proceed according to the method of Example 1, but change the acrylic acid monomer (removal of the polymerization inhibitor) into 6mmol acrylamide, change the reaction temperature to 65°C, change the stirring reaction to 8 hours, and keep the rest unchanged to obtain polystyrene- Acrylamide nanoparticles.
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Abstract
The invention discloses a polystyrene fluorescent nanometer particle as well as a preparation method and applications of the polystyrene fluorescent nanometer particle. The particle is the polystyrene fluorescent nanometer particle with a fluorescent material, wherein the fluorescent material is a chelate compound formed by the reaction of rare earth elements, beta-diketone substances and tri-n-octylphosphine oxide. The preparation method of the polystyrene fluorescent nanometer particle comprises the following steps of: (1), synthesizing the polystyrene fluorescent nanometer particle through an emulsion polymerization method, a seed emulsion polymerization method or a non-soap emulsion polymerization method; and (2), adding the polystyrene nanometer particle which is swelled by the organic solvent to the organic solvent dissolved with the fluorescent substances, wherein the organic solvent is removed after the fluorescent substances are diffused to the inner part of the polystyrene nanometer particle. The fluorescent nanometer particle has good mono-dispersion and suspension stability and is very suitable for being applied to immune-chromatographic assay.
Description
technical field [0001] The invention relates to a polystyrene nano fluorescent particle and its preparation and application, in particular to a long-lived polystyrene fluorescent nano particle and its preparation and application in immunochromatography. Background technique [0002] How to achieve rapid and accurate quantitative analysis through simple operations without relying on large-scale equipment has always been a research topic in the field of immunodiagnosis. This demand has prompted various medical testing (POCT) products that can be performed around patients roll out. [0003] Currently, conventional immunoassays mainly include various luminescent immunoassays (LIA), radioimmunoassays (RIA) and enzyme-labeled immunoassays (EIA). Both RIA and EIA are mature and stable immunoassay methods. Until now, RIA and EIA still occupy an important position in the field of immunodiagnosis. However, the markers used by RIA (commonly 125 I) has limited detectability and is di...
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