37 results about "Lupus anticoagulant" patented technology

The invention relates to an in vitro diagnostic kit for the determination of activated partial thromboplatin time (APTT) in clinical test. The invention consists of a partial thromboplatin reagent and a calcium chloride solution, which is used for the detection of the defects of the intrinsic coagulation pathway factors and the screening test of the related inhibitors, and the invention is also a primary means for the current coagulation factor and heparin anticoagulant treatment and the detection of lupus anticoagulant. The invention has the advantages of long stability time and good repeatability of the partial thromboplatin reagent after a re-dissolution, at the same time, the invention has better consistency of the measurement results of a blood coagulation analyzer by using an optical method and a magnetic bead method, therefore, the invention is applicable to large, medium and small hospitals, and the test results of different hospitals have comparability, therefore the invention has important meaning for implementing the one general report of test reports, provides the reliable experimental data for the clinical diagnosis and the treatment of diseases and improves the efficiency and value of the basic studies of thrombosis and hemostasis.

The invention discloses a lumbricus polypeptide having anticoagulant and thrombolytic effects, and an enzymatic hydrolysis preparation method and an application thereof. The preparation method comprises the steps: cleaning and drying lumbricus dried bodies, and crushing into a powder; adding water, stirring, and centrifuging to obtain a protein precipitate; adding water to dissolve the protein, adding an alkaline protease with the ratio of the enzyme volume to the protein weight of 0.1-3 mL:100 g, and stirring; exterminating enzyme activity, adding trypsin with the ratio of the enzyme mass to the protein weight of 0.5-5 g:100 g, and stirring at the temperature of 25-60 DEG C; allowing the enzymatic hydrolysate to pass through an anion exchange column, collecting a part of eluate; and then passing through a macroporous adsorption resin, carrying out separation and purification, and collecting and drying to obtain a lumbricus anticoagulant peptide powder. A new source is added for high-activity anticoagulant active substances, the product activity is high, the preparation process is convenient and feasible, and the environment is protected; and the lumbricus polypeptide can be used as a raw material of foods for thrombosis prevention, thrombolysis and blood consistency reduction, is supplemented with lactose, mannitol and the like, and is made into a powder, a tablet, a capsule or an oral liquid.

Disclosed is a method for measuring clotting time including: preparing a measurement sample by mixing a blood specimen suspected to contain a lupus anticoagulant, an activator, and a divalent-metal-ion-producing compound for facilitating formation of a phospholipid-containing complex; and measuring a clotting time after adding, to the measurement sample, an aqueous solution of a calcium salt as a coagulation initiator.

The invention discloses a reagent for screening and determining lupus anticoagulant (LA) in blood plasma by a dilution viper venom test method. The LA screening and determining reagent comprises viper venom blood clotting factor X activating agents, rapeseed phosphatide and calcium ions. The rapeseed phosphatide replaces the animal cephalin in the prior art, the proper addition quantity is low, and the rapeseed phosphatide has higher specificity and sensitivity for LA. The LA screening and determining reagent has the advantages of good specificity, high sensitivity, high stability and high detection feasibility degree.

Disclosed is a blood sample determination method including: emitting light to a measurement specimen prepared by mixing a clotting time measuring reagent and a blood sample suspected to be derived from a subject having lupus anticoagulant or a coagulation factor inhibitor, to obtain optical information about an amount of light from the measurement specimen; obtaining at least one parameter regarding derivative of clot waveform, based on the obtained optical information; and determining, based on a value of the obtained parameter, whether the blood sample is suspected to be a sample derived from a subject having lupus anticoagulant or is suspected to be a sample derived from a subject having a coagulation factor inhibitor.

The present invention provides a reagent kit for detecting LA which includes a first clotting time-measuring reagent containing manganese salt and a second clotting time-measuring reagent which contains manganese salt at a concentration lower than that of the first clotting time-measuring reagent or does not contain manganese salt and a method of determining the presence or absence of LA using the kit.

A reagent kit capable of distinguishing between a blood sample containing lupus anticoagulant and blood samples from individuals having other anticoagulant diseases is disclosed. The reagent kit comprises two coagulation time reagents containing phosphatidylserine in a different phosphatidylserine content ratio to the total content of phospholipids from each other, threreby giving different coagulation times with use of corresponding coagulation time reagent.

The invention belongs to the technical field of biological detection, and discloses a lupus anticoagulant detection reagent as well as a preparation method and a use method thereof. The lupus anticoagulant detection reagent mainly comprises a screening reagent, a confirming reagent and a compounding region, wherein the screening reagent is prepared from blood coagulation factor activators with thevolume percentage being 13mg/L, 65mmol/L of buffer solution with the pH being 7.5, 55mg/L of synthetic phospholipids, 75g/L of protein protection agents, 0.06 percent of preservatives, 100mmol/L of ion intensity regulators and 8g/L of blood coagulation factor protection agents; the conforming agent has high content of synthetic phospholipids. The reagent is a liquid reagent; the preparation process is simple and convenient; the cost is relatively low; through the addition of the protein protection agents, the stability of the liquid reagent is effectively ensured; the false positive rate andfalse negative rate in the detection result are effectively reduced; different synthetic phospholipids are respectively used on screening reagents and conforming reagents, so that the LA detection sensitivity is improved; the detection test accuracy is improved.

The invention provides a blood virus RAN protective agent and a blood sampling tube. The blood virus RAN protective agent is prepared by dissolving the following components in DEPC (diethyl pyrocarbonate) solution 100-500mg/ml glycine, 1-5mg/ml aprotinin, 1-5mg/ml wortmannin, 10-40mg/ml glutathione, 100-500mg/ml sodium thiocyanate, 1-3mg/ml anticoagulant and 5-20mg/ml membrane protective agent. The blood virus RAN protective agent provided by the invention is capable of supplying long-term protection to virus RNA in blood under room temperature, preventing degradation and pollution of virus RNA and effectively guaranteeing accuracy and validity of blood sample.

In order to provide a reagent kit for detecting LA which can clearly separate a lupus anticoagulant (LA)-positive specimen group from an LA-negative specimen group, it is configured that the reagent kit for detecting LA contains a first clotting time-measuring reagent and a second clotting time-measuring reagent and at least one of the first clotting time-measuring reagent and the second clotting time-measuring reagent contains alkali metal salt. The presence or absence of LA can be determined using the kit.

The invention relates to the technical field of clinical diagnosis, and particularly discloses a lupus anticoagulant confirmation reagent based on a dRVVT method. The confirmation reagent comprises abuffer solution, soyabean lecithin, viper venom activator RVV-X, calcium, a heparin neutralizer and a stabilizer. The lupus anticoagulant confirmation reagent (kit) of the coagulation method is provided on the basis of the principle of the dRVVT method, and comprises a buffer solution, soybean phospholipid, viper venom activator RVV-X, calcium, a heparin neutralizer, a preservative, a stabilizer and Zn<2+> salt or Mn <2+> salt. By adding metal ions Zn<2+> and Mn<2+> into the confirmation reagent, the sensitivity of the confirmation reagent is improved, so that the detection accuracy of the reagent is improved.

The invention relates to application of ginsenoside in the pharmacy field, in particular to application of ginsenoside in preparing antiphospholipid syndrome molecular targeting treatment medicine. In a laboratory antiphospholipid syndrome mouse model, the compound can effectively lower the level of anticardiolipin antibodies, anti-beta2-GP1 antibodies and lupus anticoagulants in blood; meanwhile, in a pregnant mouse model, the compound effectively lower aCL activation, shortens partial thromboplastin time and reduces the blood platelet count and the abortion rate; safety study shows that the compound is high in safety, has no influence on the central nervous system, the cardiovascular system and the respiratory system and has no mutagenic action. It is indicated that the compound has high safety and a good inhibiting effect on the antiphospholipid syndrome at the test dose and can be used for treating the antiphospholipid syndrome.

The invention relates to the technical field of biology, in particular to a lupus anticoagulant confirmation reagent and a preparation method thereof. The confirmation reagent comprises tris(hydroxymethyl) aminomethane, a blood coagulation factor X activator, rabbit cephalin, gelatin, glycine, trehalose, sodium merthiolate and calcium chloride. According to the lupus anticoagulant confirmation kit, by adjusting the concentration and components of the stabilizer, the lupus anticoagulant confirmation kit is good in stability and high in interference resistance, the reagent can be placed stably for 10 days after being redissolved at the temperature of 2-8 DEG C, and the freeze-dried powder can be placed stably for 2 years at the temperature of 2-8 DEG C. The stability of the reagent is superior to that of common commercially available products, reagents are fully utilized, reagent waste is reduced, and cost is well saved for hospitals.

The invention discloses a lupus anticoagulant detection reagent which includes a screening reagent and a diagnosis reagent. Both of the two reagents include a buffer solution, an activating agent silica, and synthetic phospholipid, wherein the content of the synthetic phospholipid is low in the screening reagent but is high in the diagnosis reagent. The synthetic phospholipid is composed of phosphatidyl serine, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl glycerol and cholesterol according to the mass ratio of 5:30:40:15:10. The lupus anticoagulant detection reagent is high in sensitivity and is a liquid reagent; compared with a DRVVT method, the lupus anticoagulant detection reagent is very small in inter-batch difference and is more easy to produce.

The invention relates to substituted polycyclic aryl and heteroaryl pyrimidinone compounds useful as inhibitors of serine proteases of the coagulation cascade and compounds, compositions and methods for anticoagulant therapy for the treatment and prevention of a variety of thrombotic conditions including coronary artery and cerebrovascular diseases.