The invention discloses an
electrochemical detection method for activating
blood coagulation time, which comprises the following specific steps: S1, fixing a detection
reagent on an
electrode substrate, pasting a membrane,
cutting the
electrode substrate into strips, and packaging a
test card finished product; s2, during detection, inserting the
test card into a coagulation analyzer, and adding ablood sample to be detected; s3, after the
blood coagulation analyzer detects that the to-be-detected blood sample enters the reaction cavity of the
test card, applying alternating
voltage, starting aheating piece of the detection module for heating, wherein the temperature is controlled to be 37 DEG C + / -0.5 DEG C; s4, in the detection process, along with solidification of the blood sample, enabling a current
signal in a reaction cavity of the test card to change, enabling a detection module of the coagulation analyzer to receive the current
signal, converting the current
signal into a
voltage signal through an
operational amplifier chip, carrying out
data acquisition and
processing, and finally, adopting a mathematical
algorithm to obtain an inflection point of a coagulation
reaction curve; and calibrating the inflection point to obtain a detection result. The
electrochemical detection method of the activated
blood coagulation time is simple and controllable to operate and can improve the detection accuracy and stability.