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Electrochemical detection method for activating blood coagulation time

A detection method and technology of coagulation time, applied in the field of medical detection, can solve problems such as delay, heparinized coagulation, large operating room costs, etc., and achieve the effect of improving performance and optimizing circuit boards

Pending Publication Date: 2020-11-03
NANJING LANSION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Administering heparin with the diatomaceous earth ACT test is ineffective because ACT is greatly delayed in the presence of aprotinin, thereby risking insufficient heparinization and subsequent intraoperative clotting, and the time it takes to prolong ACT results is relatively Large operating room costs, should be avoided if possible

Method used

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  • Electrochemical detection method for activating blood coagulation time
  • Electrochemical detection method for activating blood coagulation time
  • Electrochemical detection method for activating blood coagulation time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1: The electrochemical detection method of the activated coagulation time specifically comprises the following steps:

[0082] S1: Fix the detection reagent on the electrode substrate, cut into strips after sticking the film to make the finished blood coagulation test card, and package it;

[0083] S11: The coagulation index detection reagent is fixed;

[0084] S111: Preparation of dispensing solution for blood coagulation index test;

[0085] In the step S111, five kinds of dispensing solutions for coagulation index tests are prepared, and the specific preparation methods of the five kinds of dispensing solutions for coagulation index tests are as follows:

[0086] S1111 Preparation of liquid A: Weigh disodium hydrogen phosphate dodecahydrate and sodium dihydrogen phosphate dihydrate, dissolve them in pure water so that the dissolved concentration is 8.4%, and obtain liquid A. The phosphoric acid dodecahydrate in liquid A The mass of sodium hydrogen disodi...

Embodiment 2

[0099] Embodiment 2: The difference from Embodiment 1 is that the composition ratio and drying temperature time of the dot sol formula in the step S11 are different, and the specific step S1 includes the following steps:

[0100] S1: Fix the detection reagent on the electrode substrate, cut into strips after sticking the film to make the finished blood coagulation test card, and package it;

[0101] S11: The coagulation index detection reagent is fixed;

[0102] S111: Preparation of dispensing solution for blood coagulation index test;

[0103] In the step S111, five kinds of dispensing solutions for coagulation index tests are prepared, and the specific preparation methods of the five kinds of dispensing solutions for coagulation index tests are as follows:

[0104]S1111 Preparation of liquid A: Weigh disodium hydrogen phosphate dodecahydrate and sodium dihydrogen phosphate dihydrate, dissolve in pure water to make the dissolved concentration 5%, and obtain liquid A, the pho...

Embodiment 3

[0112] Embodiment 3: The difference from Example 1 is that the composition ratio in the dot sol formula in the step S111 is different, and specifically the step S1 includes the following steps:

[0113] S1: Fix the detection reagent on the electrode substrate, cut into strips after sticking the film to make the finished blood coagulation test card, and package it;

[0114] S11: the coagulation index detection reagent is fixed;

[0115] S111: Preparation of dispensing solution for blood coagulation index test;

[0116] In the step S111, five kinds of dispensing solutions for coagulation index tests are prepared, and the specific preparation methods of the five kinds of dispensing solutions for coagulation index tests are as follows:

[0117] S1111 Preparation of liquid A: Weigh disodium hydrogen phosphate dodecahydrate and sodium dihydrogen phosphate dihydrate, dissolve in pure water to make the dissolved concentration 15%, and obtain liquid A, the phosphoric acid dodecahydrat...

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Abstract

The invention discloses an electrochemical detection method for activating blood coagulation time, which comprises the following specific steps: S1, fixing a detection reagent on an electrode substrate, pasting a membrane, cutting the electrode substrate into strips, and packaging a test card finished product; s2, during detection, inserting the test card into a coagulation analyzer, and adding ablood sample to be detected; s3, after the blood coagulation analyzer detects that the to-be-detected blood sample enters the reaction cavity of the test card, applying alternating voltage, starting aheating piece of the detection module for heating, wherein the temperature is controlled to be 37 DEG C + / -0.5 DEG C; s4, in the detection process, along with solidification of the blood sample, enabling a current signal in a reaction cavity of the test card to change, enabling a detection module of the coagulation analyzer to receive the current signal, converting the current signal into a voltage signal through an operational amplifier chip, carrying out data acquisition and processing, and finally, adopting a mathematical algorithm to obtain an inflection point of a coagulation reaction curve; and calibrating the inflection point to obtain a detection result. The electrochemical detection method of the activated blood coagulation time is simple and controllable to operate and can improve the detection accuracy and stability.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to an electrochemical detection method for activating coagulation time. Background technique [0002] At present, the activated clotting time (ACT) test of blood or plasma is a calibration index used at home and abroad to check the clotting time during extracorporeal circulation of blood. Monitoring "ACT" can determine the dose of heparin anticoagulant and protamine antagonist required for the patient's blood, which is an important measure to prevent postoperative bleeding and embolism. ACT test can be used in bypass surgery, PTCA, ICU / CCU, ECMO, hemofiltration, angioplasty, anticoagulant monitoring of patients. The ACT test is especially important for patients receiving heparin and aprotinin therapy. [0003] With the widespread application of cardiopulmonary bypass (CPB), bleeding complications during and after cardiac surgery have attracted increasing attention. Abno...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/327
CPCG01N27/3275G01N27/416
Inventor 许行尚杰弗瑞·陈
Owner NANJING LANSION BIOTECH CO LTD
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