41 results about "Coagulation analyzers" patented technology

The invention discloses a preparation method and application of D-dimer immuno latex microspheres. In the preparation method of D-dimer immuno latex microspheres, a chemical crosslinking method is optimized, the carboxyl of polystyrene microsphere is activated in a low-pH condition and then coupled with amino of an antibody dissolved in a high-pH condition, and the mixture of the two is neutral and is coupled for 12-24h at a low temperature, so that not only can the coupling efficiency of the antibody be guaranteed, but also the damage of the antibody activity caused by an activator is reduced to a great extent. A latex enhanced turbidimetric immunoassay D-dimer test kit prepared from the immuno latex microspheres prepared by the method has the characteristics of high sensitivity, accurate quantification, good repeatability and stable property, and the lowest detection limit can reach 0.01mg / L; the latex enhanced turbidimetric immunoassay D-dimer test kit can be applied to a full-automatic biochemical analyzer or coagulation analyzer, is quick and easy to operate, only needs 5-10 minutes from detection to result collection and has relatively good clinical application prospect.

The invention discloses a detection method for heparin content. The detection method comprises the steps of mixing a sample to be tested with an FXa activity detection reagent by a developing substrate method to obtain a mixture, incubating the mixture, and detecting the signal intensity of a developing substrate; and finally calculating signal intensity and comparing the calculated signal intensity with a standard curve to obtain the heparin content of the sample to be tested, wherein the developing substrate is a developing substrate of an activated factor X (FXa) and is selected from any one of the following substrates of Benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide.HCl, CH3O-CO-D-CHA-Gly-Arg-p-nitroanilide.AcOH, Acetyl-D-Arg-Gly-Arg-p-nitroanilide.2HCl and 4-Nz-D-Arg-Gly-Arg-p-nitroanilide.2HCl. The detection method disclosed by the invention can be used for detecting general non-graded heparin, low-molecular-weight heparin and fondaparinux; furthermore, according to the detection method, the detection stability and the repetitiveness are high, the heparin content can be reflected accurately, the sensitivity and the accuracy are extremely high, and the optimal dosage range of the heparin can be found out quickly, so that a patient does not need to be detected for multiple times. Moreover, the detection method can be also used on an automatic instrument such as a coagulation analyzer or a biochemical analyzer, so that automation is realized, and clinical popularization and application can be assisted; the detection method has the characteristics of simplicity in operation, high sensitivity and high repetitiveness.

One kind blood samples detection method using magnetic beads, completed with the following steps: (1) Get fresh clinical samples; (2)Put these specimen on coagulation analyzer, use magnetic beads and special adjustment reagents aiming at the whole blood specimens, measure four coagulations: Activated partial thromboplastin time (APTT), Prothrombin time (PT), Prothrombin time (TT), Fibrinogen (FIB). The advantages of this invention are: 1.The method is simple, time-saving and easy to technical staff. 2. Reduce costs and save centrifuges and other equipment, reduce errors; 3. It is efficient and suitable for the battlefield, natural disasters and remote mountainous areas, medical teams. Particularly, it's suitable for hemorrhagic diseases and natural disasters (such as hemophilia, etc.). 4. Save specimen volume. 5. Reflect the level of specimens of blood coagulation preciously and accurately.

The invention discloses a coagulation analyzer and a coagulation analysis method, which belong to the technical field of medical apparatus and instruments. The coagulation analyzer comprises an optical coagulation analysis module, a sample interference analysis module, a magnetic coagulation analysis module and a test transfer module; according to the coagulation analyzer, the sample interference analysis module is utilized for judging whether an interfering substance in a sample influences a coagulation detection result of the optical coagulation analysis module or not; if not, the optical coagulation analysis module is utilized for carrying out coagulation analysis and reporting a detection result; if yes, the test transfer module is utilized for transferring a test to the magnetic coagulation analysis module for carrying out coagulation analysis and reporting a detection result.

The invention relates to a blood coagulation factor and fibrinolysis measuring method, which is used for testing by using a semi-automatic blood coagulation analyzer. The blood coagulation factor and fibrinolysis measuring method comprises the following testing steps of: collecting a patient blood sample and separating blood plasma out; injecting the blood plasma into a testing cup by using a sample feeding gun; putting the testing cup in a blood coagulation factor measuring device, adding a reagent, starting a timing button of the blood coagulation factor testing device, and testing the blood plasma in the testing cup by the blood coagulation factor testing device according to a double magnetic circuit magnetic bead method; and injecting the blood plasma into a fibrinolysis testing cup by the sample feeding gun, putting the fibrinolysis testing cup in a fibrinolysis testing device, adding a reagent, starting a timing button of the fibrinolysis testing device, testing the blood plasmain the fibrinolysis testing cup by the fibrinolysis testing device according to a photoelectric turbidimetry, and printing and outputting fibrinolysis data after the testing by the fibrinolysis testing device is ended. The blood coagulation factor and fibrinolysis measuring method disclosed by the invention is simple and convenient and greatly reduced in cost.

The invention relates to a measurement and analysis method for a coagulation analyzer, and the coagulation analyzer. The measurement and analysis method comprises the steps of acquiring reaction data in a reaction solution, comparing differences to find the maximum difference value, determining a reaction point corresponding to the maximum difference value as the highest reaction point, setting an initial reaction difference value or an end reaction difference value according to the maximum difference value, forward or backward searching points matching with the initial reaction difference value or the end reaction difference value according to the highest reaction point, determining a corresponding reaction point as an initial reaction point or an end reaction point; and performing subsequent analysis according to the initial reaction point and the end reaction point. The measurement and analysis method for the coagulation analyzer and the coagulation analyzer determine the initial reaction difference value or the end reaction difference value according to the highest reaction point, finds the initial reaction point and the end reaction point corresponding to the initial reaction difference value or the end reaction difference value respectively by forward or backward searching according to reaction time, thereby reducing influences on sample reaction violence degree of external environment, so that stability and accuracy of the measurement are increased and reproducibility of the sample analysis is relatively good.

The invention provides cleaning liquid of a blood coagulation analysis instrument. The cleaning liquid is prepared from sodium hypochlorite with the mass concentration being 1 percent, sodium chloride with the mass concentration being 0.6 percent, alkaline protease with the mass concentration being 0.3 to 0.5 percent, a stabilizing agent with the mass concentration being 1 percent, a surfactant with the mass concentration being 0.5 percent to 1.0 percent, layered crystal sodium disilicate with the mass concentration being 0.05 to 0.5 percent and the balance water. The invention also provides a preparation method of the cleaning liquid of the blood coagulation analysis instrument. In the cleaning liquid provided by the invention, the stabilizing agent, the surfactant and the layered crystal sodium disilicate are used in a matched way, so that the sodium hypochlorite and the alkaline protease can stably exist; in addition, the cleaning effect of the cleaning liquid is improved; in addition, the detection equipment pipeline is effectively cleaned; the accuracy of the detection result is improved. In addition, pH value regulation by phosphates is avoided, so that the environment pollution is avoided.

The invention provides a thrombelastograph coagulation analyzer and an application method thereof. The thrombelastograph coagulation analyzer comprises a movable supporting part, a fixed supporting part, a connecting part, a rotary shaft, a working magnetic body, a Hall element and a processing unit, wherein one end of the movable supporting part is fixedly connected with the rotary shaft and the other end of the movable supporting part is connected with the fixed supporting part through the connecting part; the movable supporting part is fixedly connected with the working magnetic body; the rotary shaft has rotation relative to the fixed supporting part under a driving effect of detected blood; the rotary shaft drives the movable supporting part to rotate; the movable supporting part drives the working magnetic body to move; the Hall element is connected with the processing unit; the Hall element is used for outputting a detection electric signal according to changes of a magnetic field of the working magnetic body; the processing unit is used for determining blood coagulation data of the detected blood according to the detection electric signal. According to the thrombelastograph coagulation analyzer and the application method thereof, provided by the invention, the accuracy of detecting the detected blood can be improved.

The invention discloses a coagulation analyzer based on multiple methodologies which comprises a rack module, the rack module comprises a bottom-layer installation part and an upper-layer equipment cavity, and the upper-layer equipment cavity is divided into a sample injection area and a detection area which are communicated with each other. A sample introduction frame module, a puncture arm module, a transfer trolley module and a hopper module are sequentially mounted in the sample introduction area from the first end to the second end, and a sample separation arm module is mounted between the sample introduction frame module and the transfer trolley module; an emergency trolley module, a reagent disc module, a detection module, a grabbing arm module and a light source module are sequentially mounted in the detection area from the first end to the second end, and a reagent arm module is mounted at the top of the detection area; and the light source module is connected with the detection module through an optical fiber. The coagulation analyzer has the advantages of high sensitivity, high automation degree on a reaction cup supply and detection channel module and good compatibility.

The invention provides a thrombin-like enzyme titer determination method. According to the invention, blood coagulation quality control plasma is adopted as a substrate, and determination is carried out by using a blood coagulation analyzer. With the thrombin-like enzyme, fibrinogen in the plasma can be directly hydrolyzed into fibrin, such that the plasma is coagulated. With a principle that plasma coagulation time is inversely proportional to the thrombin-like enzyme titer within a certain thrombin-like enzyme concentration range, the titer of the thrombin-like enzyme is obtained. With the method provided by the invention, product titer differences caused by different criteria of surveyors upon coagulation terminal points and differences in human-citric acid plasma are avoided. Therefore, highly efficient and accurate determinations upon thrombin-like enzyme are realized.

The invention provides a coagulation analyzer, a sample conveying device and a cup conveying method. The sample cup conveying device comprises a sample cup feeding mechanism, a sample cup bearing mechanism and a sample cup conveying mechanism, wherein the sample cup feeding mechanism is used for supplying sample cups with magnetic beads to loading positions; the sample cup bearing mechanism comprises a feeding block used for bearing the sample cups and a magnetic piece arranged on the feeding block, the feeding block has a bearing cavity, the bearing cavity is used for accommodating the samplecups supplied by the sample cup feeding mechanism at the loading position, and the magnetic piece is used for attracting magnetic beads of the sample cup located in the bearing cavity; and the samplecup conveying mechanism is used for enabling the sample cup bearing mechanism to move between the loading position and an unloading position so as to convey the sample cups from the loading positionto the unloading position. In the sample cup conveying process, the magnetic part can attract the magnetic beads in the sample cup in the bearing cavity, so that the positions of the magnetic beads are fixed, the magnetic beads are prevented from moving along the arc-shaped bottom part of the sample cup, the magnetic beads are prevented from falling off, and the detection accuracy rate is improved.

The invention discloses a scheduling method of a full-automatic coagulation analyzer. The scheduling method comprises the steps of S1 initializing a system; S2 whether a reaction cup exists in an incubation area or not is judged, if not, sending incubation information to a gripper, enabling the gripper to grip the cup from a track area to the incubation area, and executing the step S4, and if yes,executing the step S3; S3 judging whether the reaction cup exists in the detection area or not, if not, waiting until the detection information is received, grabbing the cup to the detection area from the incubation area by the gripper, and entering the step S5 after the gripper leaves the detection area; if so, entering the step S6; S4 after incubation is completed, sending out detection information, and executing the step S3 again; S5 sending reagent needle transferring information; and S6 after the system waits for receiving the cup loss information, carrying out two operations at the sametime, and after the step is completed, executing the step S2 again.

The invention discloses an electrochemical detection method for activating blood coagulation time, which comprises the following specific steps: S1, fixing a detection reagent on an electrode substrate, pasting a membrane, cutting the electrode substrate into strips, and packaging a test card finished product; s2, during detection, inserting the test card into a coagulation analyzer, and adding ablood sample to be detected; s3, after the blood coagulation analyzer detects that the to-be-detected blood sample enters the reaction cavity of the test card, applying alternating voltage, starting aheating piece of the detection module for heating, wherein the temperature is controlled to be 37 DEG C + / -0.5 DEG C; s4, in the detection process, along with solidification of the blood sample, enabling a current signal in a reaction cavity of the test card to change, enabling a detection module of the coagulation analyzer to receive the current signal, converting the current signal into a voltage signal through an operational amplifier chip, carrying out data acquisition and processing, and finally, adopting a mathematical algorithm to obtain an inflection point of a coagulation reaction curve; and calibrating the inflection point to obtain a detection result. The electrochemical detection method of the activated blood coagulation time is simple and controllable to operate and can improve the detection accuracy and stability.

The embodiment of the invention discloses a sample detection method, a coagulation analyzer and a storage medium. The method comprises the following steps: adding a reagent into an original sample toobtain a detection sample; within a set duration after the reagent is added, applying a light beam to irradiate the detection sample to obtain first optical information of scattered light and / or transmitted light after the light beam irradiates the detection sample; and obtaining the condition of the interferent in the original sample according to the first optical information. The set duration inthe process can be understood as the period of time before the fibrin-bound fibrin polymer is formed after a reagent (such as a diluent, a mixed reagent or a trigger reagent) is added, and the set duration can be preset according to experience. According to the sample detection method, a buffer reaction cup is not needed, and the user detection cost is reduced.

The invention discloses a detection method of a semi-automatic coagulation analyzer. The detection method includes the steps of: 1) starting the analyzer and entering main menu; 2) selecting detection object and preparing a reagent and a sample; 3) sucking the reagent into a detection hole and pre-heating the reagent; 4) performing zero point tracing, adding blood plasma into the detection hole filled with the reagent, and sending a measurement command to a measurement part; 5) after the measurement is completed, reading a measurement result by a host computer; 6) printing the result by a printer; 7) selecting whether the measurement is carried out continuously or not by the host computer; and 8) finishing measurement, wherein a measurement step includes the following operations: a) initializing the system when the measurement part receives the measurement command; b) waiting the compound whether the measurement is carried out by the host computer; c) regulating a signal zero point before every time that the blood plasma is added into the detection hole filled with the reagent by means of a zero point automatic set circuit, and converting a voltage signal into a digital signal through an ADC unit; and d) reading the digital signal by a MCU and performing data analysis by the MCU to obtain measurement data and diagnosis report. The method is simple in operation, has high efficiency and detection precision and is reduced in cost.

The invention provides a waste liquor discharge control method and a coagulation analyzer. A buffer storage function of a waste liquor buffer container is used, and thus when replacement or removal ofwaste liquor elimination is conducted on a waste liquor containing container, there is no need to pause the online of a new sample is not needed to be paused, so that the measurement efficiency is improved. The method comprises the steps that through a cleaning solution container, a cleaning solution is obtained through a cleaning solution container; the cleaning solution is used for cleaning a sample needle and / or an agent needle are / is used for cleaning the cleaning solution, so that waste liquor is generated; the waste liquor is controlled to be discharged into the waste liquor buffer container through a pipeline; the waste liquor of the waste liquor buffer container is controlled to be discharged into the waste liquor containing container.

The invention provides an acceleration sensor signal compensation method for a thrombelastograph coagulation analyzer. Interference of sampling noise signals is eliminated through N-point mean filtering; meanwhile, a piezoelectric sensor technology is used, a vibration signal digital model is analyzed, vibration noise caused by vibration is eliminated, and effective dynamic signals are reserved; the frequency and amplitude of vibration have periodicity and randomness, and a good effect cannot be achieved under special conditions completely through a digital signal processing technology when the vibration is overlapped with effective signals, so that when feedback signals of a piezoelectric sensor are highly related with the vibration noise, the feedback signals of the piezoelectric sensorare input to compensate the dynamic signals to achieve a better filtering effect.

The invention discloses an automatic leveling method for a thrombelastograph coagulation analyzer and a system thereof. The method specifically comprises the following steps: a three-dimensional coordinate system is established with point O as the origin, the plane of a placement platform of the thrombelastograph coagulation analyzer is recorded as the plane OAB, an objective plane is recorded asa plane OA'B', a plane XOY is recorded as the horizontal plane, and the plane OAB and the plane OA'B' are positioned inside the three-dimensional coordinate system, wherein the included angle betweena line segment OA and the horizontal plane is beta, the included angle between a line segment OB and the horizontal plane is theta, and the included angle between the plane OAB and the horizontal plane is delta; successively detecting values of theta, beta and delta, judging the relation between theta and beta, compensating different angle values for the plane OAB according to different relations,and automatically completing leveling of the plane OAB. By the automatic leveling means, misoperation is avoided, and accuracy and efficiency are enhanced.

The invention is applicable to the technical field of medical instruments, and provides a magnetic bead analysis cup, a cup grabbing system and method and a coagulation analyzer. The magnetic bead analysis cup is used for a cup grabbing system which is used in cooperation with an optical cup, and comprises a cup body, the bottom of the cup body is provided with an arc channel in which particles can be driven by a magnetic force to roll back and forth, the cup body is provided with a to-be-clamped part which is grabbed by the cup grabbing system, and the to-be-clamped part includes a protrudingpart matching a grabbing part of the cup grabbing system. The magnetic bead analysis cup and the optical cup can match the same cup grabbing system, an original specific cup grabbing system of the magnetic bead analysis cup can be omitted, the structure of the coagulation analyzer is simplified, control of the cup grabbing system is less complex, and the fault rate is reduced; and in addition, the magnetic bead analysis cup is low in production cost, easier to process and product and capable of batch production.

The invention provides a dual-wavelength multi-point test structure and method for an automatic coagulation analyzer. Compared with the existing test mechanism of the multi-channel automatic coagulation analyzer, the test modules in the invention do not need to be installed together to facilitate installation due to multiple fixed points; the adopted dual-wavelength multi-point metering is suitable for testing test discs of multiple test positions without limiting the volume of the test discs to improve the test efficiency; the first fixed metering point, the second fixed metering point, and the third fixed metering point all use the light of 450nm wavelength, and the fourth fixed metering point uses the light of 650nm wavelength, to apply more resources to projects with higher frequency of use, which makes resource allocation more reasonable, and further improves the detection efficiency.

The invention relates to a blood coagulation factor and fibrinolysis measuring method, which is used for testing by using a semi-automatic blood coagulation analyzer. The blood coagulation factor and fibrinolysis measuring method comprises the following testing steps of: collecting a patient blood sample and separating blood plasma out; injecting the blood plasma into a testing cup by using a sample feeding gun; putting the testing cup in a blood coagulation factor measuring device, adding a reagent, starting a timing button of the blood coagulation factor testing device, and testing the blood plasma in the testing cup by the blood coagulation factor testing device according to a double magnetic circuit magnetic bead method; and injecting the blood plasma into a fibrinolysis testing cup by the sample feeding gun, putting the fibrinolysis testing cup in a fibrinolysis testing device, adding a reagent, starting a timing button of the fibrinolysis testing device, testing the blood plasmain the fibrinolysis testing cup by the fibrinolysis testing device according to a photoelectric turbidimetry, and printing and outputting fibrinolysis data after the testing by the fibrinolysis testing device is ended. The blood coagulation factor and fibrinolysis measuring method disclosed by the invention is simple and convenient and greatly reduced in cost.

Provided are a method and an apparatus that enable rapid and automatic determination of the coagulant injection rate in a process of water treatment through coagulation and sedimentation. Using a coagulation analyzer comprising sample tanks 1 A to 1 D each for keeping a predetermined amount of raw water, a water supply pump 7 , water supply / discharge valves 4, 6 for raw water and washing water, mixers 3 A to 3 D, a coagulant injection unit 21 , and a detector 30 for determining the particle size and the particle number of flocs, the time within which the coagulant 20 injected into the sample tanks is dispersed by mixing and the particles begin to agglomerate (agglomeration start time) is determined, and based on the thus-determined agglomeration start time, the coagulant injection rate is determined, or the amount of the coagulant to be injected is controlled.

The invention provides a thrombin-like enzyme titer determination method. According to the invention, blood coagulation quality control plasma is adopted as a substrate, and determination is carried out by using a blood coagulation analyzer. With the thrombin-like enzyme, fibrinogen in the plasma can be directly hydrolyzed into fibrin, such that the plasma is coagulated. With a principle that plasma coagulation time is inversely proportional to the thrombin-like enzyme titer within a certain thrombin-like enzyme concentration range, the titer of the thrombin-like enzyme is obtained. With the method provided by the invention, product titer differences caused by different criteria of surveyors upon coagulation terminal points and differences in human-citric acid plasma are avoided. Therefore, highly efficient and accurate determinations upon thrombin-like enzyme are realized.

The invention discloses a preparation method and application of D-dimer immuno latex microspheres. In the preparation method of D-dimer immuno latex microspheres, a chemical crosslinking method is optimized, the carboxyl of polystyrene microsphere is activated in a low-pH condition and then coupled with amino of an antibody dissolved in a high-pH condition, and the mixture of the two is neutral and is coupled for 12-24h at a low temperature, so that not only can the coupling efficiency of the antibody be guaranteed, but also the damage of the antibody activity caused by an activator is reduced to a great extent. A latex enhanced turbidimetric immunoassay D-dimer test kit prepared from the immuno latex microspheres prepared by the method has the characteristics of high sensitivity, accurate quantification, good repeatability and stable property, and the lowest detection limit can reach 0.01mg / L; the latex enhanced turbidimetric immunoassay D-dimer test kit can be applied to a full-automatic biochemical analyzer or coagulation analyzer, is quick and easy to operate, only needs 5-10 minutes from detection to result collection and has relatively good clinical application prospect.

The invention provides an acceleration sensor signal compensation method for a thrombelastograph coagulation analyzer. Interference of sampling noise signals is eliminated through N-point mean filtering; meanwhile, a piezoelectric sensor technology is used, a vibration signal digital model is analyzed, vibration noise caused by vibration is eliminated, and effective dynamic signals are reserved; the frequency and amplitude of vibration have periodicity and randomness, and a good effect cannot be achieved under special conditions completely through a digital signal processing technology when the vibration is overlapped with effective signals, so that when feedback signals of a piezoelectric sensor are highly related with the vibration noise, the feedback signals of the piezoelectric sensorare input to compensate the dynamic signals to achieve a better filtering effect.

The invention relates to a fully-automatic coagulation analyzer, which comprises a shell. The shell is internally provided with a reaction cup feeding system, a sample and reagent conveying system, adetection system, a sample position, a reagent position, a waste cup storage mechanism, a scanning system and a host system; the reaction cup feeding system receives reaction cups from the outside through cup inlets of the shell, and thus the reaction cups are conveyed to the detection system; the sample and reagent conveying system is arranged to convey samples and reagents from the sample position and the reagent position to the detection system; the waste cup storage mechanism is arranged to receive the detected reaction cups from the detection system; and the host system comprises a human-computer interaction module and a control module. The various functional systems are reasonably partitioned, operation of all the systems is integrally controlled by a single host, a computer does notneed to be additionally configured, the space is effectively utilized, automatic detection operation is smoother, the detection speed is high, operation is easy and quick, the size is small, and thefailure rate is low.

The present invention provides an automatic loading mechanism of a fully automatic coagulation analyzer. A positioning mechanism is arranged under a disc mechanism, so that the positioning precision is greatly improved; a stepping motor is used to control an eccentric block, and a positioning link is connected to control the movement of a positioning sleeve; a piston structure is formed between the positioning sleeve and an annular cushion, and the rise and fall time of the positioning link is short, so that the time for falling into the disc is greatly saved; the loading and unloading work ofthe test disc is completed by using the loading mechanism, the height of the mechanical arm in the Z direction is completed by using the screw motor, and by using the drive motor and the belt drive to realize the rotation of the mechanical arm, angle adjustment between the mechanical arm and the test disc is completed to prepare for the gripping action, so that the whole operation process is simple and convenient; opening and closing of the first mechanical arm and the second mechanical arm are adjusted by using the electromagnet and the electromagnet rod, so that the clamping and loosening of the test disc can be completed; and the cooperation of the ball stud and the circular arc groove ensures that the test disc can be smoothly loaded.

The invention relates to a detector for a ten-channel automatic coagulation analyzer, which belongs to the technical field of medical devices. A bracket is provided, a detection board is provided on the bracket, a motor is provided at the bottom of the bracket, a plurality of V-shaped grooves are provided on the front of the detection board, and the left and right planes of the V-shaped grooves are respectively provided with The emitting light channel and the receiving light channel, the upper plane of the detection board is provided with a plurality of detection channels, and the detection board is also provided with a plurality of preheating channels. The advantages of the present invention are: a detector is provided, the detector is provided with a V-shaped groove, and an emission light channel and a light reception channel are provided, and optical methods are used for detection, and a pre-warming channel is provided on the detector to ensure blood coagulation The detection temperature control requirements of the analyzer, the invention has a simple structure and a reasonable design, and the detection channels of the detector are designed to ten channels, which improves the detection accuracy and the working efficiency of the detector, and reduces the detection cost and equipment manufacturing cost.