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Lupus anticoagulant detection kit

A technology for detection kits and lupus anticoagulants, applied in the biological field, can solve the problems of unfavorable use and storage of reagents, insufficient anti-heparin interference ability, expensive kits, etc., so as to reduce the detection cost, prolong the coagulation time, and reduce the burden. Effect

Inactive Publication Date: 2022-07-29
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stability of the lupus anticoagulant detection kit (SCT method) needs to be improved, and the validity period is not long enough, which is not conducive to customer use and reagent storage. Therefore, it is very necessary to improve the stability of the reagent
The existing lupus anticoagulant detection kit (SCT method) has insufficient anti-heparin interference ability, and the results obtained are unreliable when encountering heparin-containing samples. Therefore, it is urgent to improve the anti-heparin interference ability of the existing kits
Most of the lupus anticoagulant detection kits (SCT method) are imported from abroad, the kits are expensive, the transportation time is long, and the arrival cycle is long, which has caused a certain degree of impact on hospitals and patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A lupus anticoagulant detection kit, including SCT-S screening reagent, SCT-C confirmation reagent and SCT-CaCl 2 Solution; specific components are shown in the table below:

[0047] Table 1. Recipe of each component of the kit of Example 1

[0048]

[0049] Note: Tris buffer can be replaced with any one or more of PBS buffer, Hepes buffer, MES buffer, MOPS buffer, citrate buffer; Alanine, polyethylene glycol 6000, Tween 20 can be Replace with any one or more of span-80, BSA, HSA, gelatin, trehalose, glucose, β-cyclodextrin and mannitol; Proclin-300 can be replaced with sodium benzoate, sodium azide, gentamicin Any one or more of nitrite and nitrite; " / " means that this ingredient is not included.

Embodiment 2

[0051] A lupus anticoagulant detection kit, including SCT-S screening reagent, SCT-C confirmation reagent and SCT-CaCl 2 Solution; specific components are shown in the table below:

[0052] Table 2. Recipe of each component of the kit of Example 2

[0053]

[0054] Note: " / " means that this ingredient is not included.

Embodiment 3

[0056] A lupus anticoagulant detection kit, including SCT-S screening reagent, SCT-C confirmation reagent and SCT-CaCl 2 Solution; specific components are shown in the table below:

[0057] Table 3. Recipe of each component of the kit of Example 3

[0058]

[0059] Note: " / " means that this ingredient is not included.

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Abstract

The invention discloses a lupus anticoagulant detection kit. The lupus anticoagulant detection kit comprises an SCT-S screening reagent, an SCT-C confirmation reagent and an SCT-CaCl2 solution, the SCT-S screening reagent comprises purified silica sol, recombinant phospholipid, a first heparin neutralizer, a first buffer solution and a first auxiliary material; the SCT-C confirmation reagent comprises purified silica sol, recombinant phospholipid, a second heparin neutralizer, a second buffer solution and a second auxiliary material; the SCT-CaCl2 solution is prepared from calcium ions; the SCT-S screening reagent, the SCT-C confirmation reagent and the SCT-CaCl2 solution are in a liquid state; the content of recombinant phospholipid in the SCT-S screening reagent is lower than that of recombinant phospholipid in the SCT-C confirmation reagent; the purified silica sol and the recombinant phospholipid are matched, the reagent sensitivity is high, the positive detection rate is increased, and false negative is reduced; comprising a heparin neutralizer to eliminate the influence of heparin and improve the accuracy; the reagent is liquid, convenient to use and good in stability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a lupus anticoagulant detection kit. Background technique [0002] Lupus anticoagulant (LA) is a group of immunoglobulins that can directly bind to negatively charged phospholipids or to phospholipid protein complexes, belonging to antiphospholipid antibodies (APL). Persistent LA-positive patients are considered to have a higher risk of thrombosis and recurrence, and are also a danger signal for unexplained habitual abortion, stillbirth, thrombophilia, and certain autoimmune diseases; LA also enhances platelet aggregation and inhibits fibrinolytic activity Therefore, LA-positive patients are prone to thrombotic complications. [0003] At present, the commonly used methods for the detection of LA are activated partial thromboplastin time (APTT), modified dilute Russell viper venom time (dRVVT) and silica clotting time (SCT) assays. , the presence or absence of LA is usually judged b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/86G01N33/92
CPCG01N33/6854G01N33/92G01N33/86G01N2800/226G01N2800/36G01N2800/24G01N2800/50G01N2800/54G01N2800/56
Inventor 曹佳强胡彦勇蔡晓霞赵伟
Owner SHENZHEN DYMIND BIOTECH
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