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Reagent kit for detecting lupus anticoagulant and method of determining presence or absence of lupus anticoagulant

a technology of anticoagulant and kit, which is applied in the field ofreagent kit for detecting lupus anticoagulant and method of determining presence or absence of lupus anticoagulant, can solve the problems of difficult to clearly separate la-positive specimen groups from la-negative specimen groups, and achieve the effect of accurately determining the suppression of la clotting time and prolonging the clotting tim

Active Publication Date: 2014-06-24
SYSMEX CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]The present inventors have found that the prolongation of the clotting time by LA can be suppressed by using a clotting time-measuring reagent containing alkali metal salt so that the LA-positive specimen group can be clearly separated from the LA-negative specimen group and the present invention has been completed.
[0023]According to the reagent kit for detecting LA of the present invention, prolongation of the clotting time by LA is suppressed so that the LA-positive specimen group can be clearly separated from the LA-negative specimen group. According to the method of determining the presence or absence of LA of the present invention, it can be accurately determined whether LA is contained in the specimen obtained from the subject.

Problems solved by technology

Thus, it is difficult to clearly separate an LA-positive specimen group from an LA-negative specimen group.

Method used

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  • Reagent kit for detecting lupus anticoagulant and method of determining presence or absence of lupus anticoagulant
  • Reagent kit for detecting lupus anticoagulant and method of determining presence or absence of lupus anticoagulant
  • Reagent kit for detecting lupus anticoagulant and method of determining presence or absence of lupus anticoagulant

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examples

[0073]It was examined whether the LA-positive and LA-negative specimens can be clearly separated with a reagent for detecting LA containing alkali metal salt.

[0074]Reagents for detecting LA (two reagent systems) based on the principle of an activated partial thromboplastin time were prepared. As for the reagents for detecting LA, a reagent containing alkali metal salt and phospholipid is referred to as “LA-H” and a reagent containing phospholipid at a concentration lower than that of LA-H is referred to as “LA-L”. The reagent LA-H is composed of the first partial reagent and the second partial reagent and the reagent LA-L is composed of the third partial reagent and the fourth partial reagent.

[0075]The first partial reagent contained 50 mM of HEPES (Kishida Chemical Co., Ltd.), 0.1 mM of ellagic acid (Kishida Chemical Co., Ltd.), 25 mM of Tris (Kishida Chemical Co., Ltd.), 60 μg / ml PE (Nacalai Tesque, Inc.), 120 μg / ml PC (Nacalai Tesque, Inc.), and 20 μg / ml PS (Nacalai Tesque, Inc.)...

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Abstract

In order to provide a reagent kit for detecting LA which can clearly separate a lupus anticoagulant (LA)-positive specimen group from an LA-negative specimen group, it is configured that the reagent kit for detecting LA contains a first clotting time-measuring reagent and a second clotting time-measuring reagent and at least one of the first clotting time-measuring reagent and the second clotting time-measuring reagent contains alkali metal salt. The presence or absence of LA can be determined using the kit.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a reagent kit for detecting lupus anticoagulant which is one of the antibodies responsible for antiphospholipid syndrome and further relates to a method of determining the presence or absence of lupus anticoagulant in a specimen obtained from a subject.BACKGROUND[0002]Antiphospholipid syndrome (hereinafter, referred to as “APS”) is a general term for a group of diseases which have antiphospholipid antibodies in the blood and present clinical symptoms such as arteriovenous thrombosis and habitual abortion (see Mika Yoshida et al., The Japanese Society for Laboratory Hematology, 2008, 9 (1):69-76). Antiphospholipid antibodies (hereinafter, referred to as “aPL”) is a general term for autoantibodies which are bound to phospholipids or a complex of phospholipids and proteins (see Tatsuya Atsumi et al., The Japanese Society on Thrombosis and Hemostasis, 2008, 19 (3):329-332).[0003]There are various antibodies aPL and the name of...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G01N33/86G01N33/48
CPCG01N33/86G01N33/564G01N33/92G01N2405/04
Inventor OKUDA, MASAHIROYOSHIDA, KAZUYOKUMANO, OSAMU
Owner SYSMEX CORP
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